UniProtKB - Q13107 (UBP4_HUMAN)

BLAST

>sp|Q13107|UBP4_HUMAN Ubiquitin carboxyl-terminal hydrolase 4 OS=Homo sapiens OX=9606 GN=USP4 PE=1 SV=3
MAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVG
EHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRK
VVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERE
TRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSKSSTAPSRN
FTTSPKSSASPYSSVSASLIANGDSTSTCGMHSSGVSRGGSGFSASYNCQEPPSSHIQPG
LCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIK
QMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLEL
KDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLP
LPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAAENMVVADVYN
HRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKSRPSSTSSASAL
YGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGED
EEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADI
NSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVALRD
CIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDT
VVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDS
NVSLASEDQIVTKAAYVLFYQRRDDEFYKTPSLSSSGSSDGGTRPSSSQQGFGDDEACSM
DTN

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History
Entry version 175 (10 Oct 2018)
Sequence version 3 (17 Oct 2006)
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Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

USP4

Organism

Homo sapiens (Human)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER.5 Publications

Catalytic activityⁱ

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	311	Nucleophile	1
Metal bindingⁱ	461	Zinc	1
Metal bindingⁱ	464	Zinc	1
Metal bindingⁱ	799	Zinc	1
Metal bindingⁱ	802	Zinc	1
Active siteⁱ	881	Proton acceptorPROSITE-ProRule annotation	1

GO - Molecular functionⁱ

adenosine receptor binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 16339847
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 22001210
metal ion binding Source: UniProtKB-KW
thiol-dependent ubiquitin-specific protease activity
Source: UniProtKB
Inferred from direct assayⁱ
- 16316627
- 16472766
thiol-dependent ubiquitinyl hydrolase activity Source: Reactome

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

negative regulation of protein ubiquitination
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 16472766
protein deubiquitination
Source: UniProtKB
Inferred from direct assayⁱ
protein localization to cell surface
Source: UniProtKB
Inferred from direct assayⁱ
- 16339847
regulation of protein stability
Source: UniProtKB
Inferred from direct assayⁱ
- 16472766
spliceosomal tri-snRNP complex assembly
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 20595234
ubiquitin-dependent protein catabolic process Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protease, Thiol protease
Biological process	Ubl conjugation pathway
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-HSA-5357786 TNFR1-induced proapoptotic signaling R-HSA-5357905 Regulation of TNFR1 signaling R-HSA-5357956 TNFR1-induced NFkappaB signaling pathway R-HSA-5689880 Ub-specific processing proteases

Reactomeⁱ

R-HSA-5357786 TNFR1-induced proapoptotic signaling
R-HSA-5357905 Regulation of TNFR1 signaling
R-HSA-5357956 TNFR1-induced NFkappaB signaling pathway
R-HSA-5689880 Ub-specific processing proteases

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	C19.010

MEROPSⁱ

C19.010

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12) Alternative name(s): Deubiquitinating enzyme 4 Ubiquitin thioesterase 4 Ubiquitin-specific-processing protease 4 Ubiquitous nuclear protein homolog
Gene namesⁱ	Name:USP4 Synonyms:UNP, UNPH
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 3

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000114316.12
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:12627 USP4
MIMⁱ	603486 gene
neXtProtⁱ	NX_Q13107

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	311	C → A: Loss of activity. Its ubiquitination by TRIM21 is enhanced. 2 Publications	1
Mutagenesisⁱ	459 – 463	LVCPE → AVRPH: Reduces the interaction with RB1. 1 Publication	5
Mutagenesisⁱ	463	E → Q: Reduces the interaction with RB1. 1 Publication	1

Keywords - Diseaseⁱ

Proto-oncogene

Organism-specific databases

DisGeNET More...DisGeNETⁱ	7375
Open Targets More...OpenTargetsⁱ	ENSG00000114316
PharmGKBⁱ	PA37252

Chemistry databases

ChEMBLⁱ	CHEMBL2406900

ChEMBLⁱ

CHEMBL2406900

Polymorphism and mutation databases

BioMutaⁱ	USP4
DMDMⁱ	116242839

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000080621	1 – 963	Ubiquitin carboxyl-terminal hydrolase 4Add BLAST		963

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	655	PhosphoserineBy similarity	1
Modified residueⁱ	675	PhosphoserineBy similarity	1
Modified residueⁱ	680	PhosphoserineBy similarity	1

Post-translational modificationⁱ

Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated.2 Publications

Keywords - PTMⁱ

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q13107
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q13107
PaxDbⁱ	Q13107
PeptideAtlas More...PeptideAtlasⁱ	Q13107
PRIDEⁱ	Q13107
ProteomicsDBⁱ	59158 59159 [Q13107-2]

PTM databases

iPTMnetⁱ	Q13107
PhosphoSitePlusⁱ	Q13107

Expressionⁱ

Tissue specificityⁱ

Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung (at protein level). Expressed in the hippocampal neurons.2 Publications

Gene expression databases

Bgeeⁱ	ENSG00000114316 Expressed in 225 organ(s), highest expression level in testis
CleanExⁱ	HS_USP4
ExpressionAtlasⁱ	Q13107 baseline and differential
Genevisibleⁱ	Q13107 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA018499

HPAⁱ

HPA018499

Interactionⁱ

Subunit structureⁱ

Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with RBL1 and RBL2. Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with SART3; recruits USP4 to its substrate PRPF3.By similarity3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
itself		3	EBI-723290,EBI-723290
ADORA2A	P29274	4	EBI-723290,EBI-2902702
HUWE1	Q7Z6Z7	4	EBI-723290,EBI-625934
RIPK1	Q13546	4	EBI-723290,EBI-358507
TRIM21	P19474	3	EBI-723305,EBI-81290

GO - Molecular functionⁱ

adenosine receptor binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 16339847
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 22001210

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	113221, 116 interactors
IntActⁱ	Q13107, 32 interactors
Molecular INTeraction database More...MINTⁱ	Q13107
STRINGⁱ	9606.ENSP00000265560

Chemistry databases

BindingDBⁱ

Q13107

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	13 – 20	Combined sources	8
Helixⁱ	21 – 23	Combined sources	3
Beta strandⁱ	32 – 38	Combined sources	7
Helixⁱ	39 – 48	Combined sources	10
Helixⁱ	62 – 64	Combined sources	3
Turnⁱ	72 – 74	Combined sources	3
Beta strandⁱ	75 – 77	Combined sources	3
Turnⁱ	78 – 81	Combined sources	4
Turnⁱ	89 – 92	Combined sources	4
Beta strandⁱ	93 – 97	Combined sources	5
Helixⁱ	98 – 108	Combined sources	11
Beta strandⁱ	118 – 124	Combined sources	7
Beta strandⁱ	126 – 128	Combined sources	3
Beta strandⁱ	130 – 133	Combined sources	4
Beta strandⁱ	140 – 143	Combined sources	4
Beta strandⁱ	151 – 154	Combined sources	4
Beta strandⁱ	158 – 161	Combined sources	4
Helixⁱ	162 – 172	Combined sources	11
Beta strandⁱ	177 – 179	Combined sources	3
Beta strandⁱ	183 – 185	Combined sources	3
Beta strandⁱ	187 – 191	Combined sources	5
Beta strandⁱ	198 – 202	Combined sources	5
Turnⁱ	203 – 206	Combined sources	4
Helixⁱ	311 – 321	Combined sources	11
Helixⁱ	324 – 331	Combined sources	8
Helixⁱ	335 – 338	Combined sources	4
Helixⁱ	350 – 362	Combined sources	13
Beta strandⁱ	364 – 366	Combined sources	3
Beta strandⁱ	368 – 370	Combined sources	3
Helixⁱ	373 – 382	Combined sources	10
Helixⁱ	384 – 386	Combined sources	3
Beta strandⁱ	388 – 390	Combined sources	3
Helixⁱ	394 – 408	Combined sources	15
Helixⁱ	428 – 442	Combined sources	15
Helixⁱ	446 – 451	Combined sources	6
Beta strandⁱ	453 – 460	Combined sources	8
Turnⁱ	462 – 464	Combined sources	3
Beta strandⁱ	467 – 480	Combined sources	14
Helixⁱ	778 – 785	Combined sources	8
Beta strandⁱ	797 – 799	Combined sources	3
Turnⁱ	800 – 803	Combined sources	4
Beta strandⁱ	804 – 806	Combined sources	3
Beta strandⁱ	809 – 816	Combined sources	8
Beta strandⁱ	819 – 826	Combined sources	8
Beta strandⁱ	828 – 830	Combined sources	3
Beta strandⁱ	835 – 837	Combined sources	3
Helixⁱ	852 – 854	Combined sources	3
Beta strandⁱ	858 – 860	Combined sources	3
Beta strandⁱ	864 – 874	Combined sources	11
Beta strandⁱ	876 – 879	Combined sources	4
Beta strandⁱ	881 – 887	Combined sources	7
Turnⁱ	889 – 891	Combined sources	3
Beta strandⁱ	894 – 898	Combined sources	5
Beta strandⁱ	901 – 904	Combined sources	4
Helixⁱ	907 – 909	Combined sources	3
Beta strandⁱ	915 – 922	Combined sources	8
Helixⁱ	926 – 928	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	Q13107
SMRⁱ	Q13107
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q13107

EvolutionaryTraceⁱ

Q13107

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	11 – 122	DUSPPROSITE-ProRule annotationAdd BLAST	112
Domainⁱ	142 – 226	Ubiquitin-like 1Add BLAST	85
Domainⁱ	302 – 923	USPAdd BLAST	622
Domainⁱ	483 – 571	Ubiquitin-like 2Add BLAST	89

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	27 – 216	Necessary for interaction with SART31 PublicationAdd BLAST	190
Regionⁱ	405 – 407	Necessary for interaction with RBL2By similarity	3
Regionⁱ	459 – 463	Necessary for interaction with RB1 and RBL2By similarity	5

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	133 – 141	Nuclear export signalBy similarity		9
Motifⁱ	767 – 772	Nuclear localization signalBy similarity		6

Domainⁱ

The Ubiquitin-like domain 2 inserts into the catalytic domain and competes with the ubiquitin substrate, partially inhibiting DUB activity.1 Publication

Sequence similaritiesⁱ

Belongs to the peptidase C19 family. USP4 subfamily.Curated

Keywords - Domainⁱ

Repeat

Phylogenomic databases

eggNOGⁱ	KOG1870 Eukaryota COG5560 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00670000097750
HOGENOMⁱ	HOG000264375
HOVERGENⁱ	HBG000864
InParanoidⁱ	Q13107
KEGG Orthology (KO) More...KOⁱ	K11835
OMAⁱ	HVSMLQP
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0157
PhylomeDBⁱ	Q13107
TreeFamⁱ	TF106276

Family and domain databases

InterProⁱ	View protein in InterPro IPR035927 DUSP-like_sf IPR038765 Papain_like_cys_pep_sf IPR006615 Pept_C19_DUSP IPR001394 Peptidase_C19_UCH IPR028135 Ub_USP-typ IPR028134 USP4 IPR018200 USP_CS IPR028889 USP_dom
PANTHERⁱ	PTHR43913 PTHR43913, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06337 DUSP, 1 hit PF14836 Ubiquitin_3, 1 hit PF00443 UCH, 1 hit
SMARTⁱ	View protein in SMART SM00695 DUSP, 1 hit
SUPFAMⁱ	SSF143791 SSF143791, 1 hit SSF54001 SSF54001, 2 hits
PROSITEⁱ	View protein in PROSITE PS51283 DUSP, 1 hit PS00972 USP_1, 1 hit PS00973 USP_2, 1 hit PS50235 USP_3, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q13107-1) [UniParc]FASTA Add to basket

Also known as: UnpEL

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MAEGGGCRER PDAETQKSEL GPLMRTTLQR GAQWYLIDSR WFKQWKKYVG 
        60         70         80         90        100
FDSWDMYNVG EHNLFPGPID NSGLFSDPES QTLKEHLIDE LDYVLVPTEA 
       110        120        130        140        150
WNKLLNWYGC VEGQQPIVRK VVEHGLFVKH CKVEVYLLEL KLCENSDPTN 
       160        170        180        190        200
VLSCHFSKAD TIATIEKEMR KLFNIPAERE TRLWNKYMSN TYEQLSKLDN 
       210        220        230        240        250
TVQDAGLYQG QVLVIEPQNE DGTWPRQTLQ SKSSTAPSRN FTTSPKSSAS 
       260        270        280        290        300
PYSSVSASLI ANGDSTSTCG MHSSGVSRGG SGFSASYNCQ EPPSSHIQPG 
       310        320        330        340        350
LCGLGNLGNT CFMNSALQCL SNTAPLTDYF LKDEYEAEIN RDNPLGMKGE 
       360        370        380        390        400
IAEAYAELIK QMWSGRDAHV APRMFKTQVG RFAPQFSGYQ QQDSQELLAF 
       410        420        430        440        450
LLDGLHEDLN RVKKKPYLEL KDANGRPDAV VAKEAWENHR LRNDSVIVDT 
       460        470        480        490        500
FHGLFKSTLV CPECAKVSVT FDPFCYLTLP LPLKKDRVME VFLVPADPHC 
       510        520        530        540        550
RPTQYRVTVP LMGAVSDLCE ALSRLSGIAA ENMVVADVYN HRFHKIFQMD 
       560        570        580        590        600
EGLNHIMPRD DIFVYEVCST SVDGSECVTL PVYFRERKSR PSSTSSASAL 
       610        620        630        640        650
YGQPLLLSVP KHKLTLESLY QAVCDRISRY VKQPLPDEFG SSPLEPGACN 
       660        670        680        690        700
GSRNSCEGED EEEMEHQEEG KEQLSETEGS GEDEPGNDPS ETTQKKIKGQ 
       710        720        730        740        750
PCPKRLFTFS LVNSYGTADI NSLAADGKLL KLNSRSTLAM DWDSETRRLY 
       760        770        780        790        800
YDEQESEAYE KHVSMLQPQK KKKTTVALRD CIELFTTMET LGEHDPWYCP 
       810        820        830        840        850
NCKKHQQATK KFDLWSLPKI LVVHLKRFSY NRYWRDKLDT VVEFPIRGLN 
       860        870        880        890        900
MSEFVCNLSA RPYVYDLIAV SNHYGAMGVG HYTAYAKNKL NGKWYYFDDS 
       910        920        930        940        950
NVSLASEDQI VTKAAYVLFY QRRDDEFYKT PSLSSSGSSD GGTRPSSSQQ 
       960 
GFGDDEACSM DTN

Length:963

Mass (Da):108,565

Last modified:October 17, 2006 - v3

Checksum:ⁱ63055C9ADFE36713

Isoform 2 (identifier: Q13107-2) [UniParc]FASTA Add to basket

Also known as: UnpES

The sequence of this isoform differs from the canonical sequence as follows:
232-279: KSSTAPSRNFTTSPKSSASPYSSVSASLIANGDSTSTCGMHSSGVSRG → N

« Hide

        10         20         30         40         50
MAEGGGCRER PDAETQKSEL GPLMRTTLQR GAQWYLIDSR WFKQWKKYVG 
        60         70         80         90        100
FDSWDMYNVG EHNLFPGPID NSGLFSDPES QTLKEHLIDE LDYVLVPTEA 
       110        120        130        140        150
WNKLLNWYGC VEGQQPIVRK VVEHGLFVKH CKVEVYLLEL KLCENSDPTN 
       160        170        180        190        200
VLSCHFSKAD TIATIEKEMR KLFNIPAERE TRLWNKYMSN TYEQLSKLDN 
       210        220        230        240        250
TVQDAGLYQG QVLVIEPQNE DGTWPRQTLQ SNGSGFSASY NCQEPPSSHI 
       260        270        280        290        300
QPGLCGLGNL GNTCFMNSAL QCLSNTAPLT DYFLKDEYEA EINRDNPLGM 
       310        320        330        340        350
KGEIAEAYAE LIKQMWSGRD AHVAPRMFKT QVGRFAPQFS GYQQQDSQEL 
       360        370        380        390        400
LAFLLDGLHE DLNRVKKKPY LELKDANGRP DAVVAKEAWE NHRLRNDSVI 
       410        420        430        440        450
VDTFHGLFKS TLVCPECAKV SVTFDPFCYL TLPLPLKKDR VMEVFLVPAD 
       460        470        480        490        500
PHCRPTQYRV TVPLMGAVSD LCEALSRLSG IAAENMVVAD VYNHRFHKIF 
       510        520        530        540        550
QMDEGLNHIM PRDDIFVYEV CSTSVDGSEC VTLPVYFRER KSRPSSTSSA 
       560        570        580        590        600
SALYGQPLLL SVPKHKLTLE SLYQAVCDRI SRYVKQPLPD EFGSSPLEPG 
       610        620        630        640        650
ACNGSRNSCE GEDEEEMEHQ EEGKEQLSET EGSGEDEPGN DPSETTQKKI 
       660        670        680        690        700
KGQPCPKRLF TFSLVNSYGT ADINSLAADG KLLKLNSRST LAMDWDSETR 
       710        720        730        740        750
RLYYDEQESE AYEKHVSMLQ PQKKKKTTVA LRDCIELFTT METLGEHDPW 
       760        770        780        790        800
YCPNCKKHQQ ATKKFDLWSL PKILVVHLKR FSYNRYWRDK LDTVVEFPIR 
       810        820        830        840        850
GLNMSEFVCN LSARPYVYDL IAVSNHYGAM GVGHYTAYAK NKLNGKWYYF 
       860        870        880        890        900
DDSNVSLASE DQIVTKAAYV LFYQRRDDEF YKTPSLSSSG SSDGGTRPSS 
       910 
SQQGFGDDEA CSMDTN

Show »

Length:916

Mass (Da):103,958

Checksum:ⁱ86CBBDE6AA2AB04E

Isoform 3 (identifier: Q13107-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
233-313: SSTAPSRNFT...LGNLGNTCFM → VSFFLPRLEC...LLPQPPEWLG
314-963: Missing.

« Hide

        10         20         30         40         50
MAEGGGCRER PDAETQKSEL GPLMRTTLQR GAQWYLIDSR WFKQWKKYVG 
        60         70         80         90        100
FDSWDMYNVG EHNLFPGPID NSGLFSDPES QTLKEHLIDE LDYVLVPTEA 
       110        120        130        140        150
WNKLLNWYGC VEGQQPIVRK VVEHGLFVKH CKVEVYLLEL KLCENSDPTN 
       160        170        180        190        200
VLSCHFSKAD TIATIEKEMR KLFNIPAERE TRLWNKYMSN TYEQLSKLDN 
       210        220        230        240        250
TVQDAGLYQG QVLVIEPQNE DGTWPRQTLQ SKVSFFLPRL ECNGAILAHC 
       260        270        280        290        300
NFCLPGSSNS PASASRVAPS HLANFFFFEM ESHSVTKLEC GGAVSAYSRV 
       310 
QVMLLPQPPE WLG

Note: No experimental confirmation available.

Show »

Length:313

Mass (Da):35,661

Checksum:ⁱ7B2258A3A64AC0EB

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

H7C189	H7C189_HUMAN	Ubiquitin carboxyl-terminal hydrola... Ubiquitin carboxyl-terminal hydrolase 4	USP4	621	Annotation score:
C9JNU9	C9JNU9_HUMAN	Ubiquitin carboxyl-terminal hydrola... Ubiquitin carboxyl-terminal hydrolase 4	USP4	363	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	373	R → S in AAB72237 (PubMed:7784062).Curated		1
Sequence conflictⁱ	744	S → R in AAB72237 (PubMed:7784062).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_028180	620	Y → C. Corresponds to variant dbSNP:rs9311440Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_005258	232 – 279	KSSTA…GVSRG → N in isoform 2. 1 PublicationAdd BLAST	48
Alternative sequenceⁱVSP_044814	233 – 313	SSTAP…NTCFM → VSFFLPRLECNGAILAHCNF CLPGSSNSPASASRVAPSHL ANFFFFEMESHSVTKLECGG AVSAYSRVQVMLLPQPPEWL G in isoform 3. 1 PublicationAdd BLAST	81
Alternative sequenceⁱVSP_044815	314 – 963	Missing in isoform 3. 1 PublicationAdd BLAST	650

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U20657 mRNA Translation: AAB72237.1 AF017305 mRNA Translation: AAC27355.1 AF017306 mRNA Translation: AAC27356.1 AK291795 mRNA Translation: BAF84484.1 AC121247 Genomic DNA No translation available. BC068017 mRNA No translation available. BC125130 mRNA Translation: AAI25131.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS2793.1 [Q13107-1] CCDS2794.1 [Q13107-2] CCDS58832.1 [Q13107-3]
PIRⁱ	T09478
NCBI Reference Sequences More...RefSeqⁱ	NP_001238806.1, NM_001251877.1 [Q13107-3] NP_003354.2, NM_003363.3 [Q13107-1] NP_955475.1, NM_199443.2 [Q13107-2]
UniGeneⁱ	Hs.403828 Hs.77500

Genome annotation databases

Ensemblⁱ	ENST00000265560; ENSP00000265560; ENSG00000114316 [Q13107-1] ENST00000351842; ENSP00000341028; ENSG00000114316 [Q13107-2] ENST00000416417; ENSP00000400623; ENSG00000114316 [Q13107-3]
GeneIDⁱ	7375
KEGGⁱ	hsa:7375
UCSC genome browser More...UCSCⁱ	uc003cwq.3 human [Q13107-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q13107	Ubiquitin specific peptidase 4 (Proto-oncogene), isoform CRA_d		HUMAN		963	UniRef100_Q13107
Q13107-2	Ubiquitin specific peptidase 4 (Proto-oncogene), isoform CRA_b		HUMAN		916	UniRef100_A0A024R2X6

Protein	Similar proteins		Species	Score	Length	Source
Q13107	Ubiquitin carboxyl-terminal hydrolase 4		PONAB		963	UniRef90_Q13107
Ubiquitin carboxyl-terminal hydrolase 4		BOVIN		963
Ubiquitin specific peptidase 4 (Proto-oncogene), isoform CRA_d		HUMAN		963
ubiquitin carboxyl-terminal hydrolase 4		TARSY		975
Ubiquitin carboxyl-terminal hydrolase 4		CASCN		964
+133
Q13107-2	Ubiquitin specific peptidase 4 (Proto-oncogene), isoform CRA_b		HUMAN		916	UniRef90_A0A024R2X6
Uncharacterized protein		PROCO		918
ubiquitin carboxyl-terminal hydrolase 4 isoform X2		ODORO		917
ubiquitin carboxyl-terminal hydrolase 4 isoform X3		Enhydra lutris kenyoni		917
ubiquitin carboxyl-terminal hydrolase 4 isoform X2		NEOSC		917
+85
Q13107-3	Uncharacterized protein		NOMLE		313	UniRef90_Q13107-3
USP4 isoform 5		PANTR		312
Uncharacterized protein		PANPA		311
UPI000CEF6471		PONAB		304
UPI00062A80DA		NOMLE		304

Protein	Similar proteins		Species	Score	Length	Source
Q13107	Ubiquitin carboxyl-terminal hydrolase 4		BOVIN		963	UniRef50_Q13107
Ubiquitin carboxyl-terminal hydrolase 4		PONAB		963
Ubiquitin specific peptidase 4 (Proto-oncogene), isoform CRA_d		HUMAN		963
ubiquitin carboxyl-terminal hydrolase 4		TARSY		975
Uncharacterized protein		MUSPF		964
+206
Q13107-2	Ubiquitin carboxyl-terminal hydrolase 4	)	MOUSE		962	UniRef50_P35123
Ubiquitin carboxyl-terminal hydrolase 4		RAT		961
Uncharacterized protein		PROCO		965
Ubiquitin carboxyl-terminal hydrolase 4-like protein		CRIGR		964
ubiquitin carboxyl-terminal hydrolase 4		MESAU		963
+262
Q13107-3	Isoform 4 of Ubiquitin carboxyl-terminal hydrolase 15		HUMAN		235	UniRef50_Q9Y4E8-4
Uncharacterized protein		CALJA		247
USP15 isoform 12 (Fragment)		PANTR		239
USP15 isoform 12 (Fragment)		PONAB		239
Uncharacterized protein		MACNE		238
+119

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U20657 mRNA Translation: AAB72237.1 AF017305 mRNA Translation: AAC27355.1 AF017306 mRNA Translation: AAC27356.1 AK291795 mRNA Translation: BAF84484.1 AC121247 Genomic DNA No translation available. BC068017 mRNA No translation available. BC125130 mRNA Translation: AAI25131.1
CCDSⁱ	CCDS2793.1 [Q13107-1] CCDS2794.1 [Q13107-2] CCDS58832.1 [Q13107-3]
PIRⁱ	T09478
RefSeqⁱ	NP_001238806.1, NM_001251877.1 [Q13107-3] NP_003354.2, NM_003363.3 [Q13107-1] NP_955475.1, NM_199443.2 [Q13107-2]
UniGeneⁱ	Hs.403828 Hs.77500

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2Y6E	X-ray	2.40	A/B/C/D/E/F	296-490	[»]
				A/B/C/D/E/F	765-932	[»]
	5CTR	X-ray	3.01	C/D	1-230	[»]
ProteinModelPortalⁱ	Q13107
SMRⁱ	Q13107
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	113221, 116 interactors
IntActⁱ	Q13107, 32 interactors
MINTⁱ	Q13107
STRINGⁱ	9606.ENSP00000265560

Chemistry databases

BindingDBⁱ	Q13107
ChEMBLⁱ	CHEMBL2406900

Protein family/group databases

MEROPSⁱ	C19.010

MEROPSⁱ

C19.010

PTM databases

iPTMnetⁱ	Q13107
PhosphoSitePlusⁱ	Q13107

Polymorphism and mutation databases

BioMutaⁱ	USP4
DMDMⁱ	116242839

Proteomic databases

EPDⁱ	Q13107
MaxQBⁱ	Q13107
PaxDbⁱ	Q13107
PeptideAtlasⁱ	Q13107
PRIDEⁱ	Q13107
ProteomicsDBⁱ	59158 59159 [Q13107-2]

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000265560; ENSP00000265560; ENSG00000114316 [Q13107-1] ENST00000351842; ENSP00000341028; ENSG00000114316 [Q13107-2] ENST00000416417; ENSP00000400623; ENSG00000114316 [Q13107-3]
GeneIDⁱ	7375
KEGGⁱ	hsa:7375
UCSCⁱ	uc003cwq.3 human [Q13107-1]

Organism-specific databases

CTDⁱ	7375
DisGeNETⁱ	7375
EuPathDBⁱ	HostDB:ENSG00000114316.12
GeneCardsⁱ	USP4
HGNCⁱ	HGNC:12627 USP4
HPAⁱ	HPA018499
MIMⁱ	603486 gene
neXtProtⁱ	NX_Q13107
OpenTargetsⁱ	ENSG00000114316
PharmGKBⁱ	PA37252
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG1870 Eukaryota COG5560 LUCA
GeneTreeⁱ	ENSGT00670000097750
HOGENOMⁱ	HOG000264375
HOVERGENⁱ	HBG000864
InParanoidⁱ	Q13107
KOⁱ	K11835
OMAⁱ	HVSMLQP
OrthoDBⁱ	EOG091G0157
PhylomeDBⁱ	Q13107
TreeFamⁱ	TF106276

Enzyme and pathway databases

Reactomeⁱ	R-HSA-5357786 TNFR1-induced proapoptotic signaling R-HSA-5357905 Regulation of TNFR1 signaling R-HSA-5357956 TNFR1-induced NFkappaB signaling pathway R-HSA-5689880 Ub-specific processing proteases

Reactomeⁱ

R-HSA-5357786 TNFR1-induced proapoptotic signaling
R-HSA-5357905 Regulation of TNFR1 signaling
R-HSA-5357956 TNFR1-induced NFkappaB signaling pathway
R-HSA-5689880 Ub-specific processing proteases

Miscellaneous databases

ChiTaRSⁱ	USP4 human
EvolutionaryTraceⁱ	Q13107
GeneWikiⁱ	USP4
GenomeRNAiⁱ	7375
Protein Ontology More...PROⁱ	PR:Q13107
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000114316 Expressed in 225 organ(s), highest expression level in testis
CleanExⁱ	HS_USP4
ExpressionAtlasⁱ	Q13107 baseline and differential
Genevisibleⁱ	Q13107 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR035927 DUSP-like_sf IPR038765 Papain_like_cys_pep_sf IPR006615 Pept_C19_DUSP IPR001394 Peptidase_C19_UCH IPR028135 Ub_USP-typ IPR028134 USP4 IPR018200 USP_CS IPR028889 USP_dom
PANTHERⁱ	PTHR43913 PTHR43913, 1 hit
Pfamⁱ	View protein in Pfam PF06337 DUSP, 1 hit PF14836 Ubiquitin_3, 1 hit PF00443 UCH, 1 hit
SMARTⁱ	View protein in SMART SM00695 DUSP, 1 hit
SUPFAMⁱ	SSF143791 SSF143791, 1 hit SSF54001 SSF54001, 2 hits
PROSITEⁱ	View protein in PROSITE PS51283 DUSP, 1 hit PS00972 USP_1, 1 hit PS00973 USP_2, 1 hit PS50235 USP_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UBP4_HUMAN
Accessionⁱ	Q13107Primary (citable) accession number: Q13107 Secondary accession number(s): A8K6Y0 , C9IY91, O43452, O43453, Q08AK8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	October 17, 2006
	Last modified:	October 10, 2018
	This is version 175 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Peptidase families
Classification of peptidase families and list of entries
SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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UniProtKB - Q13107 (UBP4_HUMAN)

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Ubiquitin carboxyl-terminal hydrolase 4

USP4

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytosol

Lysosome

Nucleus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Keywords - Diseasei

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ