UniProtKB - Q13087 (PDIA2_HUMAN)
Protein disulfide-isomerase A2
PDIA2
Functioni
Catalytic activityi
- Catalyzes the rearrangement of -S-S- bonds in proteins. EC:5.3.4.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 71 | NucleophileBy similarity | 1 | |
Sitei | 72 | Contributes to redox potential valueBy similarity | 1 | |
Sitei | 73 | Contributes to redox potential valueBy similarity | 1 | |
Active sitei | 74 | NucleophileBy similarity | 1 | |
Sitei | 138 | Lowers pKa of C-terminal Cys of first active siteBy similarity | 1 | |
Active sitei | 418 | NucleophileBy similarity | 1 | |
Sitei | 419 | Contributes to redox potential valueBy similarity | 1 | |
Sitei | 420 | Contributes to redox potential valueBy similarity | 1 | |
Active sitei | 421 | NucleophileBy similarity | 1 | |
Sitei | 482 | Lowers pKa of C-terminal Cys of second active siteBy similarity | 1 |
GO - Molecular functioni
- disulfide oxidoreductase activity Source: ParkinsonsUK-UCL
- peptide disulfide oxidoreductase activity Source: UniProtKB
- protein disulfide isomerase activity Source: ProtInc
- steroid binding Source: UniProtKB-KW
GO - Biological processi
- protein folding Source: GO_Central
- protein folding in endoplasmic reticulum Source: ParkinsonsUK-UCL
- protein retention in ER lumen Source: ProtInc
- response to endoplasmic reticulum stress Source: GO_Central
Keywordsi
Molecular function | Chaperone, Isomerase |
Ligand | Lipid-binding, Steroid-binding |
Enzyme and pathway databases
BRENDAi | 5.3.4.1, 2681 |
PathwayCommonsi | Q13087 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein disulfide-isomerase A2 (EC:5.3.4.1)Alternative name(s): Pancreas-specific protein disulfide isomerase Short name: PDIp |
Gene namesi | Name:PDIA2 Synonyms:PDIP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000185615.15 |
HGNCi | HGNC:14180, PDIA2 |
MIMi | 608012, gene |
neXtProti | NX_Q13087 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen PROSITE-ProRule annotation
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- endoplasmic reticulum lumen Source: UniProtKB-SubCell
Keywords - Cellular componenti
Endoplasmic reticulumPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 18 | C → A: Impairs interchain disulfide bridge formation. 1 Publication | 1 | |
Mutagenesisi | 284 | N → Q: Increases formation of a highly stable disulfide-bonded PDIA2 dimer. 1 Publication | 1 | |
Mutagenesisi | 364 | C → A: No effect on interchain disulfide bridge formation. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 64714 |
OpenTargetsi | ENSG00000185615 |
PharmGKBi | PA33153 |
Miscellaneous databases
Pharosi | Q13087, Tbio |
Polymorphism and mutation databases
BioMutai | PDIA2 |
DMDMi | 21264492 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000034222 | 22 – 525 | Protein disulfide-isomerase A2Add BLAST | 504 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 18 | Interchain1 Publication | ||
Disulfide bondi | 71 ↔ 74 | Redox-activePROSITE-ProRule annotation | ||
Glycosylationi | 127 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 284 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 418 ↔ 421 | Redox-activePROSITE-ProRule annotation | ||
Glycosylationi | 516 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MassIVEi | Q13087 |
MaxQBi | Q13087 |
PaxDbi | Q13087 |
PeptideAtlasi | Q13087 |
PRIDEi | Q13087 |
ProteomicsDBi | 59143 [Q13087-1] 59144 [Q13087-2] |
PTM databases
GlyConnecti | 1655, 6 N-Linked glycans (1 site) |
GlyGeni | Q13087, 3 sites, 2 N-linked glycans (1 site) |
iPTMneti | Q13087 |
PhosphoSitePlusi | Q13087 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000185615, Expressed in pancreas and 131 other tissues |
ExpressionAtlasi | Q13087, baseline and differential |
Genevisiblei | Q13087, HS |
Organism-specific databases
HPAi | ENSG00000185615, Tissue enriched (pancreas) |
Interactioni
Subunit structurei
Monomer; predominantly as monomer under reducing conditions. Homodimer; disulfide-linked.
Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGGT1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.
1 PublicationBinary interactionsi
Hide detailsQ13087
With | #Exp. | IntAct |
---|---|---|
KRTAP12-1 [P59990] | 3 | EBI-1752525,EBI-10210845 |
KRTAP6-1 [Q3LI64] | 3 | EBI-1752525,EBI-12111050 |
NCK1 [P16333] | 3 | EBI-1752525,EBI-389883 |
Protein-protein interaction databases
BioGRIDi | 122240, 24 interactors |
IntActi | Q13087, 12 interactors |
MINTi | Q13087 |
STRINGi | 9606.ENSP00000219406 |
Miscellaneous databases
RNActi | Q13087, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 152 | Thioredoxin 1PROSITE-ProRule annotationAdd BLAST | 126 | |
Domaini | 367 – 496 | Thioredoxin 2PROSITE-ProRule annotationAdd BLAST | 130 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 522 – 525 | Prevents secretion from ERPROSITE-ProRule annotation | 4 |
Sequence similaritiesi
Keywords - Domaini
Redox-active center, Repeat, SignalPhylogenomic databases
eggNOGi | KOG0190, Eukaryota |
GeneTreei | ENSGT00940000161859 |
HOGENOMi | CLU_025879_1_0_1 |
InParanoidi | Q13087 |
OMAi | CKEMAQT |
OrthoDBi | 462118at2759 |
PhylomeDBi | Q13087 |
TreeFami | TF106381 |
Family and domain databases
Gene3Di | 3.40.30.10, 4 hits |
InterProi | View protein in InterPro IPR005792, Prot_disulphide_isomerase IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00085, Thioredoxin, 2 hits |
SUPFAMi | SSF52833, SSF52833, 4 hits |
TIGRFAMsi | TIGR01130, ER_PDI_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit PS00194, THIOREDOXIN_1, 2 hits PS51352, THIOREDOXIN_2, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSRQLLPVLL LLLLRASCPW GQEQGARSPS EEPPEEEIPK EDGILVLSRH
60 70 80 90 100
TLGLALREHP ALLVEFYAPW CGHCQALAPE YSKAAAVLAA ESMVVTLAKV
110 120 130 140 150
DGPAQRELAE EFGVTEYPTL KFFRNGNRTH PEEYTGPRDA EGIAEWLRRR
160 170 180 190 200
VGPSAMRLED EAAAQALIGG RDLVVIGFFQ DLQDEDVATF LALAQDALDM
210 220 230 240 250
TFGLTDRPRL FQQFGLTKDT VVLFKKFDEG RADFPVDEEL GLDLGDLSRF
260 270 280 290 300
LVTHSMRLVT EFNSQTSAKI FAARILNHLL LFVNQTLAAH RELLAGFGEA
310 320 330 340 350
APRFRGQVLF VVVDVAADNE HVLQYFGLKA EAAPTLRLVN LETTKKYAPV
360 370 380 390 400
DGGPVTAASI TAFCHAVLNG QVKPYLLSQE IPPDWDQRPV KTLVGKNFEQ
410 420 430 440 450
VAFDETKNVF VKFYAPWCTH CKEMAPAWEA LAEKYQDHED IIIAELDATA
460 470 480 490 500
NELDAFAVHG FPTLKYFPAG PGRKVIEYKS TRDLETFSKF LDNGGVLPTE
510 520
EPPEEPAAPF PEPPANSTMG SKEEL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0Y4J5 | H0Y4J5_HUMAN | Protein disulfide-isomerase A2 | PDIA2 | 227 | Annotation score: | ||
Q4TT65 | Q4TT65_HUMAN | Protein disulfide isomerase family ... | PDIA2 LA16c-314G4.2-007 | 154 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 96 | T → M in BAG58339 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 484 | L → Q in BAG58339 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048087 | 39 | P → S. Corresponds to variant dbSNP:rs45455191Ensembl. | 1 | |
Natural variantiVAR_048088 | 119 | T → R. Corresponds to variant dbSNP:rs45614840Ensembl. | 1 | |
Natural variantiVAR_048089 | 185 | E → K. Corresponds to variant dbSNP:rs419949Ensembl. | 1 | |
Natural variantiVAR_048090 | 286 | T → M. Corresponds to variant dbSNP:rs2685127Ensembl. | 1 | |
Natural variantiVAR_048091 | 382 | P → A. Corresponds to variant dbSNP:rs45529833Ensembl. | 1 | |
Natural variantiVAR_048092 | 388 | R → Q. Corresponds to variant dbSNP:rs400037Ensembl. | 1 | |
Natural variantiVAR_048093 | 502 | P → S3 PublicationsCorresponds to variant dbSNP:rs1048786Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_039292 | 181 – 183 | Missing in isoform 2. Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB127078 mRNA Translation: BAE48734.1 AE006463 Genomic DNA Translation: AAK61223.1 Z69667 Genomic DNA Translation: CAI95586.1 Z69667 Genomic DNA Translation: CAO78188.1 CH471112 Genomic DNA Translation: EAW85838.1 BC000537 mRNA Translation: AAH00537.2 BC075029 mRNA Translation: AAH75029.1 Sequence problems. U19948 mRNA Translation: AAC50401.1 Sequence problems. AK295383 mRNA Translation: BAG58339.1 Different initiation. |
CCDSi | CCDS42089.1 [Q13087-1] |
RefSeqi | NP_006840.2, NM_006849.2 [Q13087-1] |
Genome annotation databases
Ensembli | ENST00000219406; ENSP00000219406; ENSG00000185615 [Q13087-1] ENST00000404312; ENSP00000384410; ENSG00000185615 [Q13087-2] |
GeneIDi | 64714 |
KEGGi | hsa:64714 |
UCSCi | uc002cgo.2, human [Q13087-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB127078 mRNA Translation: BAE48734.1 AE006463 Genomic DNA Translation: AAK61223.1 Z69667 Genomic DNA Translation: CAI95586.1 Z69667 Genomic DNA Translation: CAO78188.1 CH471112 Genomic DNA Translation: EAW85838.1 BC000537 mRNA Translation: AAH00537.2 BC075029 mRNA Translation: AAH75029.1 Sequence problems. U19948 mRNA Translation: AAC50401.1 Sequence problems. AK295383 mRNA Translation: BAG58339.1 Different initiation. |
CCDSi | CCDS42089.1 [Q13087-1] |
RefSeqi | NP_006840.2, NM_006849.2 [Q13087-1] |
3D structure databases
SMRi | Q13087 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 122240, 24 interactors |
IntActi | Q13087, 12 interactors |
MINTi | Q13087 |
STRINGi | 9606.ENSP00000219406 |
PTM databases
GlyConnecti | 1655, 6 N-Linked glycans (1 site) |
GlyGeni | Q13087, 3 sites, 2 N-linked glycans (1 site) |
iPTMneti | Q13087 |
PhosphoSitePlusi | Q13087 |
Polymorphism and mutation databases
BioMutai | PDIA2 |
DMDMi | 21264492 |
Proteomic databases
MassIVEi | Q13087 |
MaxQBi | Q13087 |
PaxDbi | Q13087 |
PeptideAtlasi | Q13087 |
PRIDEi | Q13087 |
ProteomicsDBi | 59143 [Q13087-1] 59144 [Q13087-2] |
Protocols and materials databases
Antibodypediai | 22619, 363 antibodies |
DNASUi | 64714 |
Genome annotation databases
Ensembli | ENST00000219406; ENSP00000219406; ENSG00000185615 [Q13087-1] ENST00000404312; ENSP00000384410; ENSG00000185615 [Q13087-2] |
GeneIDi | 64714 |
KEGGi | hsa:64714 |
UCSCi | uc002cgo.2, human [Q13087-1] |
Organism-specific databases
CTDi | 64714 |
DisGeNETi | 64714 |
EuPathDBi | HostDB:ENSG00000185615.15 |
GeneCardsi | PDIA2 |
HGNCi | HGNC:14180, PDIA2 |
HPAi | ENSG00000185615, Tissue enriched (pancreas) |
MIMi | 608012, gene |
neXtProti | NX_Q13087 |
OpenTargetsi | ENSG00000185615 |
PharmGKBi | PA33153 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0190, Eukaryota |
GeneTreei | ENSGT00940000161859 |
HOGENOMi | CLU_025879_1_0_1 |
InParanoidi | Q13087 |
OMAi | CKEMAQT |
OrthoDBi | 462118at2759 |
PhylomeDBi | Q13087 |
TreeFami | TF106381 |
Enzyme and pathway databases
BRENDAi | 5.3.4.1, 2681 |
PathwayCommonsi | Q13087 |
Miscellaneous databases
BioGRID-ORCSi | 64714, 3 hits in 839 CRISPR screens |
GenomeRNAii | 64714 |
Pharosi | Q13087, Tbio |
PROi | PR:Q13087 |
RNActi | Q13087, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000185615, Expressed in pancreas and 131 other tissues |
ExpressionAtlasi | Q13087, baseline and differential |
Genevisiblei | Q13087, HS |
Family and domain databases
Gene3Di | 3.40.30.10, 4 hits |
InterProi | View protein in InterPro IPR005792, Prot_disulphide_isomerase IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00085, Thioredoxin, 2 hits |
SUPFAMi | SSF52833, SSF52833, 4 hits |
TIGRFAMsi | TIGR01130, ER_PDI_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit PS00194, THIOREDOXIN_1, 2 hits PS51352, THIOREDOXIN_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDIA2_HUMAN | |
Accessioni | Q13087Primary (citable) accession number: Q13087 Secondary accession number(s): A6ZJ64 Q9BW95 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | May 27, 2002 | |
Last modified: | December 2, 2020 | |
This is version 193 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations