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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36NAD; via amide nitrogenUniRule annotation1
Binding sitei63NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei127NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi156Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi171IronUniRule annotation1
Binding sitei224NAD; via amide nitrogenUniRule annotation1
Binding sitei234GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 56NADUniRule annotation2
Nucleotide bindingi154 – 156NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMBUR259564:G1G6A-2207-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:Mbur_2109
OrganismiMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Taxonomic identifieri259564 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides
Proteomesi
  • UP000001979 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002593801 – 258Thiamine thiazole synthaseAdd BLAST258

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi259564.Mbur_2109

Structurei

3D structure databases

ProteinModelPortaliQ12U93
SMRiQ12U93
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OMAiMWGGGMM
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

Q12U93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLDEVTISR AIIEEFSKVF LDYTDVDVAL VGGGPANLVA AKYLAEAGLK
60 70 80 90 100
TVIYEKKLAV GGGMWAGGMM FPRIVVQEDA LHILDEFGIS YHEYENGYYV
110 120 130 140 150
ANSIESVGKL ISGATSAGAE IFNLVNVEDV MIRENDEICG LVINWTAVEI
160 170 180 190 200
GKLHVDPLAI RSKVVVDGTG HPAVVCSTVQ RKVPGAKLGE LGVVGEKPMW
210 220 230 240 250
ADVGEKMLLD TTKEVYPNLY VAGMAANAVA GAPRMGPVFG GMLLSGKQVA

ELIIERLG
Length:258
Mass (Da):27,527
Last modified:August 22, 2006 - v1
Checksum:i2A22BE6F609CDBBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000300 Genomic DNA Translation: ABE52983.1
RefSeqiWP_011500122.1, NC_007955.1

Genome annotation databases

EnsemblBacteriaiABE52983; ABE52983; Mbur_2109
GeneIDi3998191
KEGGimbu:Mbur_2109

Similar proteinsi

Entry informationi

Entry nameiTHI4_METBU
AccessioniPrimary (citable) accession number: Q12U93
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: August 22, 2006
Last modified: July 18, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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