UniProtKB - Q12983 (BNIP3_HUMAN)
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
BNIP3
Functioni
Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.
2 PublicationsGO - Molecular functioni
- GTPase binding Source: BHF-UCL
- identical protein binding Source: IntAct
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- apoptotic process Source: MGI
- autophagic cell death Source: Ensembl
- autophagy of mitochondrion Source: ParkinsonsUK-UCL
- brown fat cell differentiation Source: Ensembl
- cardiac muscle cell apoptotic process Source: Ensembl
- cell death Source: UniProtKB
- cellular response to cobalt ion Source: BHF-UCL
- cellular response to hydrogen peroxide Source: Ensembl
- cellular response to hypoxia Source: BHF-UCL
- cellular response to mechanical stimulus Source: UniProtKB
- cerebral cortex development Source: Ensembl
- defense response to virus Source: UniProtKB
- granzyme-mediated programmed cell death signaling pathway Source: GO_Central
- intrinsic apoptotic signaling pathway in response to hypoxia Source: BHF-UCL
- mitochondrial fragmentation involved in apoptotic process Source: BHF-UCL
- mitochondrial outer membrane permeabilization Source: UniProtKB
- mitochondrial protein catabolic process Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of cell death Source: MGI
- negative regulation of membrane potential Source: UniProtKB
- negative regulation of mitochondrial fusion Source: BHF-UCL
- negative regulation of mitochondrial membrane permeability involved in apoptotic process Source: Ensembl
- negative regulation of mitochondrial membrane potential Source: Ensembl
- negative regulation of reactive oxygen species metabolic process Source: Ensembl
- neuron apoptotic process Source: UniProtKB
- oligodendrocyte differentiation Source: Ensembl
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of autophagy Source: UniProtKB
- positive regulation of autophagy of mitochondrion Source: Ensembl
- positive regulation of cardiac muscle cell apoptotic process Source: Ensembl
- positive regulation of macroautophagy Source: MGI
- positive regulation of mitochondrial calcium ion concentration Source: Ensembl
- positive regulation of mitochondrial fission Source: BHF-UCL
- positive regulation of necrotic cell death Source: Ensembl
- positive regulation of programmed cell death Source: UniProtKB
- positive regulation of protein-containing complex disassembly Source: BHF-UCL
- positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL
- reactive oxygen species metabolic process Source: UniProtKB
- regulation of aerobic respiration Source: Ensembl
- regulation of mitochondrial membrane permeability Source: UniProtKB
- regulation of programmed cell death Source: GO_Central
- response to axon injury Source: Ensembl
- response to bacterium Source: Ensembl
- response to hyperoxia Source: Ensembl
- response to hypoxia Source: UniProtKB
- response to oxygen-glucose deprivation Source: Ensembl
- toxin transport Source: Ensembl
Keywordsi
Biological process | Apoptosis, Host-virus interaction |
Enzyme and pathway databases
PathwayCommonsi | Q12983 |
SignaLinki | Q12983 |
SIGNORi | Q12983 |
Protein family/group databases
TCDBi | 1.A.20.1.1, the bcl2/adenovirus e1b-interacting protein 3 (bnip3) family |
Names & Taxonomyi
Protein namesi | Recommended name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 |
Gene namesi | Name:BNIP3 Synonyms:NIP3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1084, BNIP3 |
MIMi | 603293, gene |
neXtProti | NX_Q12983 |
VEuPathDBi | HostDB:ENSG00000176171 |
Subcellular locationi
Mitochondrion
Note: Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane.
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Mitochondrion
- integral component of mitochondrial outer membrane Source: UniProtKB
- mitochondrial membrane Source: UniProtKB
- mitochondrial outer membrane Source: UniProtKB
- mitochondrion Source: UniProtKB
Nucleus
- nuclear envelope Source: UniProtKB
- nucleoplasm Source: UniProtKB
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- dendrite Source: UniProtKB
- postsynaptic density Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 229 – 249 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Organism-specific databases
DisGeNETi | 664 |
OpenTargetsi | ENSG00000176171 |
PharmGKBi | PA25394 |
Miscellaneous databases
Pharosi | Q12983, Tbio |
Genetic variation databases
BioMutai | BNIP3 |
DMDMi | 20532402 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064964 | 1 – 259 | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3Add BLAST | 259 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 119 | PhosphoserineCombined sources | 1 | |
Modified residuei | 131 | PhosphoserineCombined sources | 1 | |
Modified residuei | 151 | PhosphoserineCombined sources | 1 | |
Modified residuei | 157 | PhosphoserineBy similarity | 1 | |
Modified residuei | 160 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q12983 |
jPOSTi | Q12983 |
MassIVEi | Q12983 |
PaxDbi | Q12983 |
PeptideAtlasi | Q12983 |
PRIDEi | Q12983 |
ProteomicsDBi | 59081 |
PTM databases
iPTMneti | Q12983 |
PhosphoSitePlusi | Q12983 |
Expressioni
Gene expression databases
Bgeei | ENSG00000176171, Expressed in endothelial cell and 245 other tissues |
ExpressionAtlasi | Q12983, baseline and differential |
Genevisiblei | Q12983, HS |
Organism-specific databases
HPAi | ENSG00000176171, Tissue enhanced (pancreas, skeletal muscle) |
Interactioni
Subunit structurei
Homodimer. Binds to BCL2.
Interacts with BNIP3L and ACAA2.
Interacts (via BH3 domain) with SPATA18 (via coiled-coil domains).
Interacts with BOK; promotes BOK oligomerization (PubMed:15868100).
5 Publications(Microbial infection) Interacts with adenovirus E1B 19 kDa protein.
1 Publication(Microbial infection) Interacts with Epstein-Barr virus BHRF1.
1 PublicationBinary interactionsi
Q12983
GO - Molecular functioni
- GTPase binding Source: BHF-UCL
- identical protein binding Source: IntAct
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 107132, 94 interactors |
DIPi | DIP-34429N |
IntActi | Q12983, 67 interactors |
MINTi | Q12983 |
STRINGi | 9606.ENSP00000357625 |
Miscellaneous databases
RNActi | Q12983, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q12983 |
SMRi | Q12983 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q12983 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 20 – 78 | DisorderedSequence analysisAdd BLAST | 59 | |
Regioni | 117 – 167 | DisorderedSequence analysisAdd BLAST | 51 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 165 – 190 | BH3Add BLAST | 26 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 61 – 78 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 125 – 146 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 147 – 167 | Basic and acidic residuesSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QQ4B, Eukaryota |
GeneTreei | ENSGT00390000013415 |
InParanoidi | Q12983 |
OMAi | MYNGDME |
OrthoDBi | 1250566at2759 |
PhylomeDBi | Q12983 |
TreeFami | TF315424 |
Family and domain databases
InterProi | View protein in InterPro IPR010548, BNIP3 |
PANTHERi | PTHR15186, PTHR15186, 1 hit |
Pfami | View protein in Pfam PF06553, BNIP3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGDAAADPPG PALPCEFLRP GCGAPLSPGA QLGRGAPTSA FPPPAAEAHP
60 70 80 90 100
AARRGLRSPQ LPSGAMSQNG APGMQEESLQ GSWVELHFSN NGNGGSVPAS
110 120 130 140 150
VSIYNGDMEK ILLDAQHESG RSSSKSSHCD SPPRSQTPQD TNRASETDTH
160 170 180 190 200
SIGEKNSSQS EEDDIERRKE VESILKKNSD WIWDWSSRPE NIPPKEFLFK
210 220 230 240 250
HPKRTATLSM RNTSVMKKGG IFSAEFLKVF LPSLLLSHLL AIGLGIYIGR
RLTTSTSTF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6RP06 | F6RP06_HUMAN | BCL2/adenovirus E1B 19 kDa protein-... | BNIP3 | 219 | Annotation score: | ||
A0A0J9YW18 | A0A0J9YW18_HUMAN | BCL2/adenovirus E1B 19 kDa protein-... | BNIP3 | 243 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 68 | Q → E in AAC00022 (PubMed:7954800).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY886764 Genomic DNA Translation: AAW62256.1 Different initiation. AL162274 Genomic DNA No translation available. BC009342 mRNA No translation available. BC021989 mRNA Translation: AAH21989.1 Different initiation. AF002697 mRNA Translation: AAC16738.1 Different initiation. U15174 mRNA Translation: AAC00022.1 Different initiation. |
PIRi | I38865 |
RefSeqi | NP_004043.3, NM_004052.3 |
Genome annotation databases
Ensembli | ENST00000368636; ENSP00000357625; ENSG00000176171 |
GeneIDi | 664 |
KEGGi | hsa:664 |
MANE-Selecti | ENST00000368636.9; ENSP00000357625.6; NM_004052.4; NP_004043.4 |
UCSCi | uc001lkv.2, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY886764 Genomic DNA Translation: AAW62256.1 Different initiation. AL162274 Genomic DNA No translation available. BC009342 mRNA No translation available. BC021989 mRNA Translation: AAH21989.1 Different initiation. AF002697 mRNA Translation: AAC16738.1 Different initiation. U15174 mRNA Translation: AAC00022.1 Different initiation. |
PIRi | I38865 |
RefSeqi | NP_004043.3, NM_004052.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2J5D | NMR | - | A/B | 211-255 | [»] | |
2KA1 | NMR | - | A/B | 219-253 | [»] | |
2KA2 | NMR | - | A/B | 219-253 | [»] | |
BMRBi | Q12983 | |||||
SMRi | Q12983 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107132, 94 interactors |
DIPi | DIP-34429N |
IntActi | Q12983, 67 interactors |
MINTi | Q12983 |
STRINGi | 9606.ENSP00000357625 |
Protein family/group databases
TCDBi | 1.A.20.1.1, the bcl2/adenovirus e1b-interacting protein 3 (bnip3) family |
PTM databases
iPTMneti | Q12983 |
PhosphoSitePlusi | Q12983 |
Genetic variation databases
BioMutai | BNIP3 |
DMDMi | 20532402 |
Proteomic databases
EPDi | Q12983 |
jPOSTi | Q12983 |
MassIVEi | Q12983 |
PaxDbi | Q12983 |
PeptideAtlasi | Q12983 |
PRIDEi | Q12983 |
ProteomicsDBi | 59081 |
Protocols and materials databases
Antibodypediai | 31, 601 antibodies from 35 providers |
DNASUi | 664 |
Genome annotation databases
Ensembli | ENST00000368636; ENSP00000357625; ENSG00000176171 |
GeneIDi | 664 |
KEGGi | hsa:664 |
MANE-Selecti | ENST00000368636.9; ENSP00000357625.6; NM_004052.4; NP_004043.4 |
UCSCi | uc001lkv.2, human |
Organism-specific databases
CTDi | 664 |
DisGeNETi | 664 |
GeneCardsi | BNIP3 |
HGNCi | HGNC:1084, BNIP3 |
HPAi | ENSG00000176171, Tissue enhanced (pancreas, skeletal muscle) |
MIMi | 603293, gene |
neXtProti | NX_Q12983 |
OpenTargetsi | ENSG00000176171 |
PharmGKBi | PA25394 |
VEuPathDBi | HostDB:ENSG00000176171 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QQ4B, Eukaryota |
GeneTreei | ENSGT00390000013415 |
InParanoidi | Q12983 |
OMAi | MYNGDME |
OrthoDBi | 1250566at2759 |
PhylomeDBi | Q12983 |
TreeFami | TF315424 |
Enzyme and pathway databases
PathwayCommonsi | Q12983 |
SignaLinki | Q12983 |
SIGNORi | Q12983 |
Miscellaneous databases
BioGRID-ORCSi | 664, 122 hits in 1042 CRISPR screens |
ChiTaRSi | BNIP3, human |
EvolutionaryTracei | Q12983 |
GeneWikii | BNIP3 |
GenomeRNAii | 664 |
Pharosi | Q12983, Tbio |
PROi | PR:Q12983 |
RNActi | Q12983, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000176171, Expressed in endothelial cell and 245 other tissues |
ExpressionAtlasi | Q12983, baseline and differential |
Genevisiblei | Q12983, HS |
Family and domain databases
InterProi | View protein in InterPro IPR010548, BNIP3 |
PANTHERi | PTHR15186, PTHR15186, 1 hit |
Pfami | View protein in Pfam PF06553, BNIP3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BNIP3_HUMAN | |
Accessioni | Q12983Primary (citable) accession number: Q12983 Secondary accession number(s): O14620, Q96GP0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | June 7, 2017 | |
Last modified: | February 23, 2022 | |
This is version 199 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families