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Protein

Transcription initiation factor TFIID subunit 10

Gene

TAF10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors.

GO - Molecular functioni

  • DNA binding Source: Ensembl
  • enzyme binding Source: UniProtKB
  • estrogen receptor binding Source: UniProtKB
  • RNA polymerase binding Source: UniProtKB
  • thiol-dependent ubiquitinyl hydrolase activity Source: Reactome
  • transcription coactivator activity Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-3214847 HATs acetylate histones
R-HSA-5689880 Ub-specific processing proteases
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
SABIO-RKiQ12962

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 10
Alternative name(s):
STAF28
Transcription initiation factor TFIID 30 kDa subunit
Short name:
TAF(II)30
Short name:
TAFII-30
Short name:
TAFII30
Gene namesi
Name:TAF10
Synonyms:TAF2A, TAF2H, TAFII30
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000166337.9
HGNCiHGNC:11543 TAF10
MIMi600475 gene
neXtProtiNX_Q12962

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi189K → Q: Abolishes methylation. Does not affect interaction with LOXL2 but greatly reduces deamination by LOXL2. 2 Publications1

Organism-specific databases

OpenTargetsiENSG00000166337
PharmGKBiPA36318

Polymorphism and mutation databases

BioMutaiTAF10
DMDMi3024688

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001188972 – 218Transcription initiation factor TFIID subunit 10Add BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei44PhosphoserineCombined sources1
Modified residuei48PhosphothreonineCombined sources1
Modified residuei189Allysine; alternate1 Publication1
Modified residuei189N6,N6,N6-trimethyllysine; alternate3 Publications1

Post-translational modificationi

Monomethylated at Lys-189 by SETD7, leading to increased affinity for RNA polymerase II.2 Publications
Lysine deamination at Lys-189 to form allysine is mediated by LOXL2. Allysine formation by LOXL2 results in release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ12962
MaxQBiQ12962
PaxDbiQ12962
PeptideAtlasiQ12962
PRIDEiQ12962
ProteomicsDBi59056

PTM databases

iPTMnetiQ12962
PhosphoSitePlusiQ12962

Expressioni

Gene expression databases

BgeeiENSG00000166337 Expressed in 223 organ(s), highest expression level in right testis
CleanExiHS_TAF10
ExpressionAtlasiQ12962 baseline and differential
GenevisibleiQ12962 HS

Organism-specific databases

HPAiCAB022405
HPA004148

Interactioni

Subunit structurei

TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TBP is not part of TFTC. Component of the PCAF complex, at least composed of TADA2L/ADA2, TADA3L/ADA3, SUPT3H, TAF5L TAF6L, TAF9, TAF10, TAF12 and TRRAP (PubMed:9885574). Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H, TAF2, TAF4, TAF5, GCN5L2/GCN5, TAF10 and TRRAP (PubMed:10373431, PubMed:12601814). Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9 (PubMed:11564863, PubMed:18206972). The STAGA core complex is associated with a subcomplex required for histone deubiquitination composed of ATXN7L3, ENY2 and USP22. Interacts with TAF3 (PubMed:11438666). Interacts with LOXL2 (PubMed:25959397).8 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112744, 75 interactors
ComplexPortaliCPX-900 SAGA complex
CPX-903 TFTC histone acetylation complex
CPX-915 General transcription factor complex TFIID
CPX-930 General transcription factor complex TFIID, TAF4B variant
CPX-989 PCAF histone acetylase complex
CORUMiQ12962
DIPiDIP-297N
IntActiQ12962, 23 interactors
MINTiQ12962
STRINGi9606.ENSP00000299424

Structurei

Secondary structure

1218
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ12962
SMRiQ12962
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi187 – 189[KR]-[STA]-K motif1 Publication3

Domaini

The [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase.1 Publication

Sequence similaritiesi

Belongs to the TAF10 family.Curated

Phylogenomic databases

eggNOGiKOG3423 Eukaryota
COG5162 LUCA
GeneTreeiENSGT00390000009368
HOGENOMiHOG000285966
HOVERGENiHBG079227
InParanoidiQ12962
KOiK03134
OMAiCIANDNA
OrthoDBiEOG091G0UWA
PhylomeDBiQ12962
TreeFamiTF313156

Family and domain databases

CDDicd07982 TAF10, 1 hit
InterProiView protein in InterPro
IPR003923 TFIID_30kDa
PANTHERiPTHR21242 PTHR21242, 1 hit
PfamiView protein in Pfam
PF03540 TFIID_30kDa, 1 hit
PIRSFiPIRSF017246 TFIID_TAF10, 1 hit
PRINTSiPR01443 TFIID30KDSUB

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q12962-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSCSGSGADP EAAPASAASA PGPAPPVSAP AALPSSTAAE NKASPAGTAG
60 70 80 90 100
GPGAGAAAGG TGPLAARAGE PAERRGAAPV SAGGAAPPEG AISNGVYVLP
110 120 130 140 150
SAANGDVKPV VSSTPLVDFL MQLEDYTPTI PDAVTGYYLN RAGFEASDPR
160 170 180 190 200
IIRLISLAAQ KFISDIANDA LQHCKMKGTA SGSSRSKSKD RKYTLTMEDL
210
TPALSEYGIN VKKPHYFT
Length:218
Mass (Da):21,711
Last modified:November 1, 1996 - v1
Checksum:i032ED5CA97EBE411
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WWP5A0A087WWP5_HUMAN
Transcription initiation factor TFI...
TAF10
115Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01370692I → T1 PublicationCorresponds to variant dbSNP:rs3176311Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13991 mRNA Translation: AAA62230.1
U25816 Genomic DNA Translation: AAB61242.1
AF498312 Genomic DNA Translation: AAM14627.1
CR541943 mRNA Translation: CAG46741.1
CH471064 Genomic DNA Translation: EAW68687.1
BC012088 mRNA Translation: AAH12088.1
CCDSiCCDS7769.1
PIRiA57694
RefSeqiNP_006275.1, NM_006284.3
UniGeneiHs.5158

Genome annotation databases

EnsembliENST00000299424; ENSP00000299424; ENSG00000166337
GeneIDi6881
KEGGihsa:6881
UCSCiuc001mej.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13991 mRNA Translation: AAA62230.1
U25816 Genomic DNA Translation: AAB61242.1
AF498312 Genomic DNA Translation: AAM14627.1
CR541943 mRNA Translation: CAG46741.1
CH471064 Genomic DNA Translation: EAW68687.1
BC012088 mRNA Translation: AAH12088.1
CCDSiCCDS7769.1
PIRiA57694
RefSeqiNP_006275.1, NM_006284.3
UniGeneiHs.5158

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2F69X-ray1.30B186-195[»]
3M53X-ray1.85B186-195[»]
3M54X-ray1.60B186-195[»]
3M55X-ray1.55B186-195[»]
3M56X-ray1.65B186-195[»]
3M57X-ray1.70B186-195[»]
3M58X-ray1.40B186-195[»]
3M59X-ray1.70B186-195[»]
3M5AX-ray1.75B186-195[»]
4J7FX-ray1.59B186-195[»]
4J7IX-ray2.56B186-195[»]
4J83X-ray1.70B186-195[»]
4J8OX-ray1.63B186-195[»]
4WV4X-ray1.91A112-212[»]
5EG2X-ray1.55B186-195[»]
ProteinModelPortaliQ12962
SMRiQ12962
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112744, 75 interactors
ComplexPortaliCPX-900 SAGA complex
CPX-903 TFTC histone acetylation complex
CPX-915 General transcription factor complex TFIID
CPX-930 General transcription factor complex TFIID, TAF4B variant
CPX-989 PCAF histone acetylase complex
CORUMiQ12962
DIPiDIP-297N
IntActiQ12962, 23 interactors
MINTiQ12962
STRINGi9606.ENSP00000299424

PTM databases

iPTMnetiQ12962
PhosphoSitePlusiQ12962

Polymorphism and mutation databases

BioMutaiTAF10
DMDMi3024688

Proteomic databases

EPDiQ12962
MaxQBiQ12962
PaxDbiQ12962
PeptideAtlasiQ12962
PRIDEiQ12962
ProteomicsDBi59056

Protocols and materials databases

DNASUi6881
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299424; ENSP00000299424; ENSG00000166337
GeneIDi6881
KEGGihsa:6881
UCSCiuc001mej.3 human

Organism-specific databases

CTDi6881
EuPathDBiHostDB:ENSG00000166337.9
GeneCardsiTAF10
HGNCiHGNC:11543 TAF10
HPAiCAB022405
HPA004148
MIMi600475 gene
neXtProtiNX_Q12962
OpenTargetsiENSG00000166337
PharmGKBiPA36318
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3423 Eukaryota
COG5162 LUCA
GeneTreeiENSGT00390000009368
HOGENOMiHOG000285966
HOVERGENiHBG079227
InParanoidiQ12962
KOiK03134
OMAiCIANDNA
OrthoDBiEOG091G0UWA
PhylomeDBiQ12962
TreeFamiTF313156

Enzyme and pathway databases

ReactomeiR-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-3214847 HATs acetylate histones
R-HSA-5689880 Ub-specific processing proteases
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
SABIO-RKiQ12962

Miscellaneous databases

ChiTaRSiTAF10 human
GeneWikiiTAF10
GenomeRNAii6881
PROiPR:Q12962
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166337 Expressed in 223 organ(s), highest expression level in right testis
CleanExiHS_TAF10
ExpressionAtlasiQ12962 baseline and differential
GenevisibleiQ12962 HS

Family and domain databases

CDDicd07982 TAF10, 1 hit
InterProiView protein in InterPro
IPR003923 TFIID_30kDa
PANTHERiPTHR21242 PTHR21242, 1 hit
PfamiView protein in Pfam
PF03540 TFIID_30kDa, 1 hit
PIRSFiPIRSF017246 TFIID_TAF10, 1 hit
PRINTSiPR01443 TFIID30KDSUB
ProtoNetiSearch...

Entry informationi

Entry nameiTAF10_HUMAN
AccessioniPrimary (citable) accession number: Q12962
Secondary accession number(s): O00703, Q13175, Q6FH13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 193 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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