UniProtKB - Q12933 (TRAF2_HUMAN)
Protein
TNF receptor-associated factor 2
Gene
TRAF2
Organism
Homo sapiens (Human)
Status
Functioni
Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.17 Publications
Catalytic activityi
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
Activity regulationi
Has very low E3 ubiquitin ligase activity in the absence of sphingosine-1-phosphate. E3 ubiquitin ligase activity is strongly activated by cytoplasmic sphingosine-1-phosphate.1 Publication
Pathwayi: protein ubiquitination
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Zinc fingeri | 34 – 73 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 | |
| Zinc fingeri | 124 – 180 | TRAF-type 1PROSITE-ProRule annotationAdd BLAST | 57 | |
| Zinc fingeri | 177 – 233 | TRAF-type 2PROSITE-ProRule annotationAdd BLAST | 57 |
GO - Molecular functioni
- CD40 receptor binding Source: BHF-UCL
- enzyme binding Source: BHF-UCL
- identical protein binding Source: IntAct
- mitogen-activated protein kinase kinase kinase binding Source: Ensembl
- protein-containing complex binding Source: Ensembl
- protein kinase binding Source: ParkinsonsUK-UCL
- protein phosphatase binding Source: UniProtKB
- sphingolipid binding Source: UniProtKB
- thioesterase binding Source: UniProtKB
- tumor necrosis factor receptor binding Source: UniProtKB
- ubiquitin protein ligase binding Source: UniProtKB
- ubiquitin-protein transferase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- activation of cysteine-type endopeptidase activity involved in apoptotic process Source: Reactome
- activation of NF-kappaB-inducing kinase activity Source: UniProtKB
- cellular protein-containing complex assembly Source: BHF-UCL
- cellular response to nitric oxide Source: Ensembl
- death-inducing signaling complex assembly Source: Reactome
- I-kappaB kinase/NF-kappaB signaling Source: Reactome
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: Reactome
- negative regulation of glial cell apoptotic process Source: Ensembl
- negative regulation of neuron death Source: ParkinsonsUK-UCL
- positive regulation of DNA-binding transcription factor activity Source: BHF-UCL
- positive regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
- positive regulation of I-kappaB phosphorylation Source: Ensembl
- positive regulation of interleukin-2 production Source: UniProtKB
- positive regulation of JUN kinase activity Source: UniProtKB
- positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
- positive regulation of protein homodimerization activity Source: UniProtKB
- positive regulation of T cell cytokine production Source: UniProtKB
- positive regulation of tumor necrosis factor-mediated signaling pathway Source: Ensembl
- programmed necrotic cell death Source: Ensembl
- protein autoubiquitination Source: UniProtKB
- protein catabolic process Source: Ensembl
- protein-containing complex assembly Source: ProtInc
- protein deubiquitination Source: Reactome
- protein heterooligomerization Source: Ensembl
- protein homotrimerization Source: UniProtKB
- protein K63-linked ubiquitination Source: UniProtKB
- regulation of apoptotic process Source: UniProtKB
- regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: Reactome
- regulation of immunoglobulin secretion Source: Ensembl
- regulation of tumor necrosis factor-mediated signaling pathway Source: Reactome
- response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
- signal transduction Source: ProtInc
- tumor necrosis factor-mediated signaling pathway Source: UniProtKB
Keywordsi
| Molecular function | Transferase |
| Biological process | Apoptosis, Ubl conjugation pathway |
| Ligand | Lipid-binding, Metal-binding, Zinc |
Enzyme and pathway databases
| Reactomei | R-HSA-140534 via Death Receptors in the presence of ligand R-HSA-3371378 Regulation by c-FLIP R-HSA-5213460 RIPK1-mediated regulated necrosis R-HSA-5218900 CASP8 activity is inhibited R-HSA-5357786 TNFR1-induced proapoptotic signaling R-HSA-5357905 Regulation of TNFR1 signaling R-HSA-5357956 TNFR1-induced NFkappaB signaling pathway R-HSA-5668541 TNFR2 non-canonical NF-kB pathway R-HSA-5675482 Regulation of necroptotic cell death R-HSA-5676594 TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway R-HSA-5689880 Ub-specific processing proteases R-HSA-69416 Dimerization of procaspase-8 R-HSA-75893 TNF signaling R-HSA-933541 TRAF6 mediated IRF7 activation R-HSA-933542 TRAF6 mediated NF-kB activation |
| SignaLinki | Q12933 |
| SIGNORi | Q12933 |
| UniPathwayi | UPA00143 |
Names & Taxonomyi
| Protein namesi | Recommended name: TNF receptor-associated factor 2 (EC:2.3.2.27)Alternative name(s): E3 ubiquitin-protein ligase TRAF2 RING-type E3 ubiquitin transferase TRAF2Curated Tumor necrosis factor type 2 receptor-associated protein 3 |
| Gene namesi | Name:TRAF2 Synonyms:TRAP3 |
| Organismi | Homo sapiens (Human) |
| Taxonomic identifieri | 9606 [NCBI] |
| Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
| Proteomesi |
|
Organism-specific databases
| EuPathDBi | HostDB:ENSG00000127191.17 |
| HGNCi | HGNC:12032 TRAF2 |
| MIMi | 601895 gene |
| neXtProti | NX_Q12933 |
Pathology & Biotechi
Mutagenesis
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Mutagenesisi | 11 | S → A: Reduces global phosphorylation. Partial reduction of TNF-dependent activation of NF-kappa-B and activation of JNK. 1 Publication | 1 | |
| Mutagenesisi | 11 | S → D: Slight increase of TNF-dependent activation of NF-kappa-B and activation of JNK. 1 Publication | 1 | |
| Mutagenesisi | 31 | K → R: Abolishes 'Lys-63'-linked polyubiquitination. | 1 | |
| Mutagenesisi | 117 | T → A: Loss of phosphorylation site. Abolishes activation of NF-kappa-B. 1 Publication | 1 | |
| Mutagenesisi | 285 | I → A: Strongly reduced interaction with BIRC3. 1 Publication | 1 | |
| Mutagenesisi | 288 | V → A: Strongly reduced interaction with BIRC3. 1 Publication | 1 | |
| Mutagenesisi | 292 | E → A: Strongly reduced interaction with BIRC3. 1 Publication | 1 |
Organism-specific databases
| DisGeNETi | 7186 |
| OpenTargetsi | ENSG00000127191 |
| PharmGKBi | PA164742666 |
Polymorphism and mutation databases
| BioMutai | TRAF2 |
| DMDMi | 23503103 |
PTM / Processingi
Molecule processing
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Initiator methioninei | RemovedCombined sources | |||
| ChainiPRO_0000056399 | 2 – 501 | TNF receptor-associated factor 2Add BLAST | 500 |
Amino acid modifications
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
| Modified residuei | 5 | PhosphoserineCombined sources | 1 | |
| Modified residuei | 7 | PhosphothreonineCombined sources | 1 | |
| Modified residuei | 11 | PhosphoserineCombined sources1 Publication | 1 | |
| Modified residuei | 22 | PhosphothreonineCombined sources | 1 | |
| Cross-linki | 31 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
| Modified residuei | 117 | Phosphothreonine; by PKC1 Publication | 1 |
Post-translational modificationi
Phosphorylated at several serine residues within the first 128 amino acid residues. Phosphorylated at Thr-117 in response to signaling via TNF and TNFRSF1A. Phosphorylation at Thr-117 is required for 'Lys-63'-linked polyubiquitination, but not for 'Lys-48'-linked polyubiquitination. Phosphorylation at Thr-117 is important for interaction with IKKA and IKKB, activation of IKK and subsequent activation of NF-kappa-B.1 Publication
Undergoes both 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination. Polyubiquitinated via 'Lys-63'-linked ubiquitin in response to TNF signaling; this requires prior phosphorylation at Thr-117. 'Lys-63'-linked polyubiquitination promotes TRAF2-mediated activation of NF-kappa-B. Can be polyubiquitinated at several Lys residues via 'Lys-48'-linked ubiquitin chains in response to TNF signaling, leading to proteasomal degradation. Autoubiquitinated, leading to its subsequent proteasomal degradation. Polyubiquitinated by BIRC2 and SIAH2, leading to its subsequent proteasomal degradation. Deubiquitinated by CYLD, a protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains.1 Publication
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
| EPDi | Q12933 |
| MaxQBi | Q12933 |
| PaxDbi | Q12933 |
| PeptideAtlasi | Q12933 |
| PRIDEi | Q12933 |
| ProteomicsDBi | 59035 59036 [Q12933-2] 59037 [Q12933-3] 59038 [Q12933-4] |
PTM databases
| iPTMneti | Q12933 |
| PhosphoSitePlusi | Q12933 |
Expressioni
Gene expression databases
| Bgeei | ENSG00000127191 Expressed in 156 organ(s), highest expression level in cerebellar hemisphere |
| CleanExi | HS_TRAF2 |
| ExpressionAtlasi | Q12933 baseline and differential |
| Genevisiblei | Q12933 HS |
Organism-specific databases
| HPAi | CAB004603 HPA009972 HPA010634 |
Interactioni
Subunit structurei
Homotrimer (PubMed:8069916). Heterotrimer with TRAF1 (PubMed:8069916). Heterotrimer with TRAF3 (via TRAF domain) (PubMed:15383523, PubMed:20447407). The domain containing the RING-type and the first TRAF-type zinc finger can also form homodimers (in vitro) (PubMed:19810754). Interacts with TNFRSF1B/TNFR2 (PubMed:7639698, PubMed:8069916, PubMed:10206649). Interacts with TNFRSF5/CD40 (PubMed:9718306). Interacts with TNFRSF4, TNFRSF7/CD27, TNFRSF8/CD30, TNFRSF9/CD137, TNFRSF11A/RANK, TNFRSF13B/TACI, TNFRSF14, TNFRSF16/NGFR, TNFRSF17/BCMA, TNFRSF18/AITR, TNFRSF19/TROY, TNFRSF19L/RELT, EDAR and IL15RA (PubMed:8627180, PubMed:9153189, PubMed:9692890, PubMed:9488716, PubMed:9774460, PubMed:9607925, PubMed:9418902, PubMed:10463949, PubMed:10037686, PubMed:10514511, PubMed:10809768, PubMed:10880535, PubMed:11035039, PubMed:10411888). Stimulation of TNF-alpha receptor TNFRSF1A leads to the formation of two distinct signaling complexes. Plasma membrane-bound complex I is composed of TNFRSF1A, TRADD, RIPK1, TRAF2 and BIRC2/c-IAP1 or BIRC3 which interacts with CHUCK/IKK-alpha, IKBKB/IKK-beta and IKBKG/IKK-gamma promoting cell survival (PubMed:21307340, PubMed:18981220). Subsequently, TRADD, RIPK1 and TRAF2 dissociate from TNFRSF1A and form cytoplasmic complex II with FADD and caspase CASP8 promoting cell apoptosis (PubMed:21307340). Interacts with TRADD (PubMed:10892748). Identified in a complex with TNFRSF1A, RIPK1 and IKBKB/IKK-beta (PubMed:18981220). Interacts with RIPK2 (PubMed:9705938). Interacts with BIRC2 and BIRC3 N-terminus; a single BIRC2 or BIRC3 molecule interacts with a heterotrimer formed by TRAF1 and TRAF2, or a TRAF2 homotrimer (PubMed:11907583, PubMed:19506082, PubMed:20447407, PubMed:20385093). Identified in a complex composed of TRAF2, TRAF3, BIRC2 and BIRC3 (By similarity). Interacts with BIRC2; the interaction promotes BIRC2 stability (PubMed:19506082). Interaction with BIRC2 and/or BIRC3 is essential for ubiquitination of IKBKE, degradation of NFKBIA and activation of NF-kappa-B (By similarity). Within complex I, phosphorylated TRAF2 interacts (via 'Lys-63'-linked polyubiquitin chains) with CHUCK/IKK-alpha, IKBKB/IKK-beta, IKBKG/IKK-gamma TAB2, TAB3 and TAK1 in response to TNF-alpha stimulation (PubMed:19150425). Within complex I, interacts with UXT isoform 1 (via TPQE motif); the interaction prevents the recruitment of FADD and CASP8/caspase 8 to complex I (PubMed:21307340). Forms a complex composed of TNFRSF8/CD30 or TNFRSF1B/TNFR2, and TRAF1, TRAF2 and E3 ligase TRAIP (PubMed:9104814). Within the complex, interacts with TRAIP; the interaction inhibits TRAF2-mediated NF-kappa B activation (PubMed:9104814). Component of a complex composed of TANK and TBK1 (PubMed:10581243). Interacts with TRPC4AP (By similarity). Interacts with MAP3K1/MEKK1, MAP3K5/ASK1 and MAP3K11/MLK3 in response to TNF-alpha stimulation; the interaction leads to JNK activation (PubMed:10346818, PubMed:19918265, PubMed:9774977). Component of a complex composed of MAP3K14/NIK BIRC3 and TRAF3; the interaction leads to BIRC2/3-mediated ubiquitination of TRAF3 upon CD40 engagement in a TRAF2-dependent manner (By similarity). Interacts with MAP3K14/NIK in response to TNF-alpha stimulation; the interaction leads to NF-kappa B activation (PubMed:9020361). Interacts with PEG3; the interaction may promote TRAF2-mediated NF-kappa B activation (By similarity). Interacts with HIVEP3; the interaction may inhibit TNF-alpha-TRAF2-mediated NF-kappa B and JNK activation (By similarity). Interacts with TANK/ITRAF; the interaction prevents interaction between TNFRSF1B/TNFR2 and TRAF2 (PubMed:8710854). Interacts with deubiquitinating enzyme CYLD; the interaction results in the deubiquitination and inactivation of TRAF2 (PubMed:12917691). Interacts with SIAH2; the interaction leads to TRAF2 ubiquitination and degradation (PubMed:12411493). Interacts with E2 conjugating enzyme UBE2N/Ubc13, E3 ligase ITCH and RNF11 in response to TNF-alpha stimulation (By similarity). Interacts with ubiquitin-editing enzyme TNFAIP3/A20 in response to TNF-alpha stimulation; the interaction promotes TRAF2 dissociation from UBE2N/Ubc13, ITCH, RNF11 and TAX1BP1 and prevents prolonged TRAF-2 ubiquitination (By similarity). Interacts with TAX1BP1 in response to TNF-alpha stimulation; the interaction promotes TRAF2 dissociation from UBE2N/Ubc13 and TNFAIP3/A20, and prevents prolonged TRAF-2 ubiquitination (By similarity). Interacts (via C-terminus) with EIF2AK2/PKR (via the kinase catalytic domain) (PubMed:15121867). Interacts with deubiquitinating enzyme USP48 (PubMed:16214042). Interacts with PTPN2; probably involved in TNF-mediated signaling (PubMed:15696169). Interacts with Toll-like receptor TLR4/3 adapter TICAM1/TRIF; the interaction may promotes TICAM1 ubiquitination (PubMed:20047764). Interacts with kinase/endoribonuclease ERN1/IRE1 and DAB2IP in response to ER stress; the interaction requires DAB2IP (By similarity). Interacts with ERN1/IRE1 and TAOK3 in response to ER stress; the interaction may promote TRAF2 phosphorylation (PubMed:11278723). Interacts (via zinc fingers) with DAB2IP (via C-terminus PER domain)in response to TNF-alpha stimulation (PubMed:15310755, PubMed:17389591). Interacts with CASP8AP2/FLASH (By similarity). Interacts with NFATC2IP; the interaction may repress IL-4 production in T cells (By similarity). Interacts with kinase CDK9 (PubMed:9827693). Interacts with sphingosine kinase 1 SPHK1 (PubMed:20577214). Interacts with kinase TNIK (PubMed:10521462). Interacts with TRAFD1 (By similarity). Interacts with DNA phosphodiesterase TDP2 (PubMed:10764746). Interacts with MAVS/IPS1 (PubMed:16153868). Interacts with CARD14 (PubMed:21302310). Interacts with Epstein-Barr virus LMP1/BNFL1 (PubMed:10411888). Interacts with GPS2 (By similarity). Interacts with XPNPEP3 (PubMed:25609706). Interacts with RIPK3 (PubMed:29883609). Interacts with RELL2 (PubMed:19969290).By similarity54 Publications
Binary interactionsi
GO - Molecular functioni
- CD40 receptor binding Source: BHF-UCL
- enzyme binding Source: BHF-UCL
- identical protein binding Source: IntAct
- mitogen-activated protein kinase kinase kinase binding Source: Ensembl
- protein kinase binding Source: ParkinsonsUK-UCL
- protein phosphatase binding Source: UniProtKB
- thioesterase binding Source: UniProtKB
- tumor necrosis factor receptor binding Source: UniProtKB
- ubiquitin protein ligase binding Source: UniProtKB
Protein-protein interaction databases
| BioGridi | 113038, 359 interactors |
| CORUMi | Q12933 |
| DIPi | DIP-6223N |
| ELMi | Q12933 |
| IntActi | Q12933, 361 interactors |
| MINTi | Q12933 |
| STRINGi | 9606.ENSP00000247668 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
| ProteinModelPortali | Q12933 |
| SMRi | Q12933 |
| ModBasei | Search... |
| MobiDBi | Search... |
Miscellaneous databases
| EvolutionaryTracei | Q12933 |
Family & Domainsi
Domains and Repeats
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Domaini | 351 – 496 | MATHPROSITE-ProRule annotationAdd BLAST | 146 |
Region
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Regioni | 283 – 293 | Important for interaction with BIRC2 and BIRC3By similarityAdd BLAST | 11 |
Coiled coil
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Coiled coili | 299 – 348 | 1 PublicationAdd BLAST | 50 |
Domaini
The coiled coil domain mediates homo- and hetero-oligomerization.1 Publication
The MATH/TRAF domain binds to receptor cytoplasmic domains.1 Publication
The RING-type zinc finger domain is essential for E3 ubiquitin-protein ligase activity. It is not essential for the stabilization of BIRC2, or for the ubiquitination of RIPK1 in response to TNFR1 signaling.1 Publication
Sequence similaritiesi
Zinc finger
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Zinc fingeri | 34 – 73 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 | |
| Zinc fingeri | 124 – 180 | TRAF-type 1PROSITE-ProRule annotationAdd BLAST | 57 | |
| Zinc fingeri | 177 – 233 | TRAF-type 2PROSITE-ProRule annotationAdd BLAST | 57 |
Keywords - Domaini
Coiled coil, Repeat, Zinc-fingerPhylogenomic databases
| eggNOGi | ENOG410ISDM Eukaryota ENOG4111M70 LUCA |
| GeneTreei | ENSGT00550000074359 |
| HOGENOMi | HOG000231558 |
| HOVERGENi | HBG058222 |
| InParanoidi | Q12933 |
| KOi | K03173 |
| OMAi | KFQDHVR |
| OrthoDBi | EOG091G0GHD |
| PhylomeDBi | Q12933 |
| TreeFami | TF321154 |
Family and domain databases
| CDDi | cd03778 MATH_TRAF2, 1 hit |
| Gene3Di | 2.60.210.10, 1 hit 3.30.40.10, 3 hits |
| InterProi | View protein in InterPro IPR002083 MATH/TRAF_dom IPR012227 TNF_rcpt--assoc_TRAF IPR008974 TRAF-like IPR027133 TRAF2 IPR037305 TRAF2_MATH IPR032070 TRAF_BIRC3-bd IPR018957 Znf_C3HC4_RING-type IPR001841 Znf_RING IPR013083 Znf_RING/FYVE/PHD IPR017907 Znf_RING_CS IPR001293 Znf_TRAF |
| PANTHERi | PTHR10131:SF21 PTHR10131:SF21, 1 hit |
| Pfami | View protein in Pfam PF16673 TRAF_BIRC3_bd, 1 hit PF00097 zf-C3HC4, 1 hit PF02176 zf-TRAF, 1 hit |
| PIRSFi | PIRSF015614 TRAF, 1 hit |
| SMARTi | View protein in SMART SM00061 MATH, 1 hit SM00184 RING, 1 hit |
| SUPFAMi | SSF49599 SSF49599, 1 hit |
| PROSITEi | View protein in PROSITE PS50144 MATH, 1 hit PS00518 ZF_RING_1, 1 hit PS50089 ZF_RING_2, 1 hit PS50145 ZF_TRAF, 2 hits |
Sequences (4+)i
Sequence statusi: Complete.
Sequence processingi: The displayed sequence is further processed into a mature form.
This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q12933-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MAAASVTPPG SLELLQPGFS KTLLGTKLEA KYLCSACRNV LRRPFQAQCG
60 70 80 90 100
HRYCSFCLAS ILSSGPQNCA ACVHEGIYEE GISILESSSA FPDNAARREV
110 120 130 140 150
ESLPAVCPSD GCTWKGTLKE YESCHEGRCP LMLTECPACK GLVRLGEKER
160 170 180 190 200
HLEHECPERS LSCRHCRAPC CGADVKAHHE VCPKFPLTCD GCGKKKIPRE
210 220 230 240 250
KFQDHVKTCG KCRVPCRFHA IGCLETVEGE KQQEHEVQWL REHLAMLLSS
260 270 280 290 300
VLEAKPLLGD QSHAGSELLQ RCESLEKKTA TFENIVCVLN REVERVAMTA
310 320 330 340 350
EACSRQHRLD QDKIEALSSK VQQLERSIGL KDLAMADLEQ KVLEMEASTY
360 370 380 390 400
DGVFIWKISD FARKRQEAVA GRIPAIFSPA FYTSRYGYKM CLRIYLNGDG
410 420 430 440 450
TGRGTHLSLF FVVMKGPNDA LLRWPFNQKV TLMLLDQNNR EHVIDAFRPD
460 470 480 490 500
VTSSSFQRPV NDMNIASGCP LFCPVSKMEA KNSYVRDDAI FIKAIVDLTG
L
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket| B1AMX8 | B1AMX8_HUMAN | TNF receptor-associated factor 2 | TRAF2 | 193 | Annotation score: | ||
| B1AMX7 | B1AMX7_HUMAN | TNF receptor-associated factor 2 | TRAF2 | 171 | Annotation score: | ||
| B1AMY1 | B1AMY1_HUMAN | TNF receptor-associated factor 2 | TRAF2 | 121 | Annotation score: |
Experimental Info
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Sequence conflicti | 205 – 310 | Missing in BAB70792 (PubMed:14702039).CuratedAdd BLAST | 106 | |
| Sequence conflicti | 343 – 365 | LEMEA…FARKR → RPFQAQCGHRYCSFCLASIL RKL in AAA87706 (PubMed:7639698).CuratedAdd BLAST | 23 |
Alternative sequence
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Alternative sequenceiVSP_039687 | 53 – 63 | Missing in isoform 3. 1 PublicationAdd BLAST | 11 | |
| Alternative sequenceiVSP_007401 | 122 | E → EVKMPACGMVTEAPAVGSRP RSPSSYDLVLHVPLTGAEAC LMSVEEETELLLR in isoform 2. 1 Publication | 1 | |
| Alternative sequenceiVSP_039688 | 176 – 200 | Missing in isoform 4. 1 PublicationAdd BLAST | 25 |
Sequence databases
| Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U12597 mRNA Translation: AAA87706.1 AK054686 mRNA Translation: BAB70792.1 AK289722 mRNA Translation: BAF82411.1 AK298370 mRNA Translation: BAG60609.1 BX538160 mRNA Translation: CAD98040.1 AY623660 Genomic DNA Translation: AAT27320.1 AL355987 Genomic DNA No translation available. AL449425 Genomic DNA No translation available. CH471090 Genomic DNA Translation: EAW88299.1 BC032410 mRNA Translation: AAH32410.1 BC033810 mRNA Translation: AAH33810.1 BC043492 mRNA Translation: AAH43492.1 BC064662 mRNA Translation: AAH64662.1 |
| CCDSi | CCDS7013.1 [Q12933-1] |
| PIRi | S56163 |
| RefSeqi | NP_066961.2, NM_021138.3 [Q12933-1] XP_011517276.1, XM_011518974.2 [Q12933-1] XP_011517277.1, XM_011518975.2 XP_011517278.1, XM_011518976.2 [Q12933-1] XP_011517279.1, XM_011518977.2 [Q12933-1] XP_011517280.1, XM_011518978.2 XP_016870584.1, XM_017015095.1 |
| UniGenei | Hs.522506 |
Genome annotation databases
| Ensembli | ENST00000247668; ENSP00000247668; ENSG00000127191 [Q12933-1] |
| GeneIDi | 7186 |
| KEGGi | hsa:7186 |
| UCSCi | uc004cjv.4 human [Q12933-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
| SeattleSNPs |
Sequence databases
| Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U12597 mRNA Translation: AAA87706.1 AK054686 mRNA Translation: BAB70792.1 AK289722 mRNA Translation: BAF82411.1 AK298370 mRNA Translation: BAG60609.1 BX538160 mRNA Translation: CAD98040.1 AY623660 Genomic DNA Translation: AAT27320.1 AL355987 Genomic DNA No translation available. AL449425 Genomic DNA No translation available. CH471090 Genomic DNA Translation: EAW88299.1 BC032410 mRNA Translation: AAH32410.1 BC033810 mRNA Translation: AAH33810.1 BC043492 mRNA Translation: AAH43492.1 BC064662 mRNA Translation: AAH64662.1 |
| CCDSi | CCDS7013.1 [Q12933-1] |
| PIRi | S56163 |
| RefSeqi | NP_066961.2, NM_021138.3 [Q12933-1] XP_011517276.1, XM_011518974.2 [Q12933-1] XP_011517277.1, XM_011518975.2 XP_011517278.1, XM_011518976.2 [Q12933-1] XP_011517279.1, XM_011518977.2 [Q12933-1] XP_011517280.1, XM_011518978.2 XP_016870584.1, XM_017015095.1 |
| UniGenei | Hs.522506 |
3D structure databases
| Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
| 1CA4 | X-ray | 2.20 | A/B/C/D/E/F | 334-501 | [»] | |
| 1CA9 | X-ray | 2.30 | A/B/C/D/E/F | 310-501 | [»] | |
| 1CZY | X-ray | 2.00 | A/B/C | 334-501 | [»] | |
| 1CZZ | X-ray | 2.70 | A/B/C | 315-501 | [»] | |
| 1D00 | X-ray | 2.00 | A/B/C/D/E/F/G/H | 334-501 | [»] | |
| 1D01 | X-ray | 2.00 | A/B/C/D/E/F | 334-501 | [»] | |
| 1D0A | X-ray | 2.00 | A/B/C/D/E/F | 334-501 | [»] | |
| 1D0J | X-ray | 2.50 | A/B/C/D/E/F | 334-501 | [»] | |
| 1F3V | X-ray | 2.00 | B | 331-501 | [»] | |
| 1QSC | X-ray | 2.40 | A/B/C | 311-501 | [»] | |
| 3KNV | X-ray | 1.90 | A | 1-133 | [»] | |
| 3M06 | X-ray | 2.67 | A/B/C/D/E/F | 266-329 | [»] | |
| 3M0A | X-ray | 2.61 | A/B/C | 266-329 | [»] | |
| 3M0D | X-ray | 2.80 | A/B | 266-329 | [»] | |
| ProteinModelPortali | Q12933 | |||||
| SMRi | Q12933 | |||||
| ModBasei | Search... | |||||
| MobiDBi | Search... | |||||
Protein-protein interaction databases
| BioGridi | 113038, 359 interactors |
| CORUMi | Q12933 |
| DIPi | DIP-6223N |
| ELMi | Q12933 |
| IntActi | Q12933, 361 interactors |
| MINTi | Q12933 |
| STRINGi | 9606.ENSP00000247668 |
PTM databases
| iPTMneti | Q12933 |
| PhosphoSitePlusi | Q12933 |
Polymorphism and mutation databases
| BioMutai | TRAF2 |
| DMDMi | 23503103 |
Proteomic databases
| EPDi | Q12933 |
| MaxQBi | Q12933 |
| PaxDbi | Q12933 |
| PeptideAtlasi | Q12933 |
| PRIDEi | Q12933 |
| ProteomicsDBi | 59035 59036 [Q12933-2] 59037 [Q12933-3] 59038 [Q12933-4] |
Protocols and materials databases
| DNASUi | 7186 |
| Structural Biology Knowledgebase | Search... |
Genome annotation databases
| Ensembli | ENST00000247668; ENSP00000247668; ENSG00000127191 [Q12933-1] |
| GeneIDi | 7186 |
| KEGGi | hsa:7186 |
| UCSCi | uc004cjv.4 human [Q12933-1] |
Organism-specific databases
| CTDi | 7186 |
| DisGeNETi | 7186 |
| EuPathDBi | HostDB:ENSG00000127191.17 |
| GeneCardsi | TRAF2 |
| H-InvDBi | HIX0169360 |
| HGNCi | HGNC:12032 TRAF2 |
| HPAi | CAB004603 HPA009972 HPA010634 |
| MIMi | 601895 gene |
| neXtProti | NX_Q12933 |
| OpenTargetsi | ENSG00000127191 |
| PharmGKBi | PA164742666 |
| GenAtlasi | Search... |
Phylogenomic databases
| eggNOGi | ENOG410ISDM Eukaryota ENOG4111M70 LUCA |
| GeneTreei | ENSGT00550000074359 |
| HOGENOMi | HOG000231558 |
| HOVERGENi | HBG058222 |
| InParanoidi | Q12933 |
| KOi | K03173 |
| OMAi | KFQDHVR |
| OrthoDBi | EOG091G0GHD |
| PhylomeDBi | Q12933 |
| TreeFami | TF321154 |
Enzyme and pathway databases
| UniPathwayi | UPA00143 |
| Reactomei | R-HSA-140534 via Death Receptors in the presence of ligand R-HSA-3371378 Regulation by c-FLIP R-HSA-5213460 RIPK1-mediated regulated necrosis R-HSA-5218900 CASP8 activity is inhibited R-HSA-5357786 TNFR1-induced proapoptotic signaling R-HSA-5357905 Regulation of TNFR1 signaling R-HSA-5357956 TNFR1-induced NFkappaB signaling pathway R-HSA-5668541 TNFR2 non-canonical NF-kB pathway R-HSA-5675482 Regulation of necroptotic cell death R-HSA-5676594 TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway R-HSA-5689880 Ub-specific processing proteases R-HSA-69416 Dimerization of procaspase-8 R-HSA-75893 TNF signaling R-HSA-933541 TRAF6 mediated IRF7 activation R-HSA-933542 TRAF6 mediated NF-kB activation |
| SignaLinki | Q12933 |
| SIGNORi | Q12933 |
Miscellaneous databases
| ChiTaRSi | TRAF2 human |
| EvolutionaryTracei | Q12933 |
| GeneWikii | TRAF2 |
| GenomeRNAii | 7186 |
| PROi | PR:Q12933 |
| SOURCEi | Search... |
Gene expression databases
| Bgeei | ENSG00000127191 Expressed in 156 organ(s), highest expression level in cerebellar hemisphere |
| CleanExi | HS_TRAF2 |
| ExpressionAtlasi | Q12933 baseline and differential |
| Genevisiblei | Q12933 HS |
Family and domain databases
| CDDi | cd03778 MATH_TRAF2, 1 hit |
| Gene3Di | 2.60.210.10, 1 hit 3.30.40.10, 3 hits |
| InterProi | View protein in InterPro IPR002083 MATH/TRAF_dom IPR012227 TNF_rcpt--assoc_TRAF IPR008974 TRAF-like IPR027133 TRAF2 IPR037305 TRAF2_MATH IPR032070 TRAF_BIRC3-bd IPR018957 Znf_C3HC4_RING-type IPR001841 Znf_RING IPR013083 Znf_RING/FYVE/PHD IPR017907 Znf_RING_CS IPR001293 Znf_TRAF |
| PANTHERi | PTHR10131:SF21 PTHR10131:SF21, 1 hit |
| Pfami | View protein in Pfam PF16673 TRAF_BIRC3_bd, 1 hit PF00097 zf-C3HC4, 1 hit PF02176 zf-TRAF, 1 hit |
| PIRSFi | PIRSF015614 TRAF, 1 hit |
| SMARTi | View protein in SMART SM00061 MATH, 1 hit SM00184 RING, 1 hit |
| SUPFAMi | SSF49599 SSF49599, 1 hit |
| PROSITEi | View protein in PROSITE PS50144 MATH, 1 hit PS00518 ZF_RING_1, 1 hit PS50089 ZF_RING_2, 1 hit PS50145 ZF_TRAF, 2 hits |
| ProtoNeti | Search... |
Entry informationi
| Entry namei | TRAF2_HUMAN | |
| Accessioni | Q12933Primary (citable) accession number: Q12933 Secondary accession number(s): A8K107 Q96NT2 | |
| Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
| Last sequence update: | September 19, 2002 | |
| Last modified: | November 7, 2018 | |
| This is version 224 of the entry and version 2 of the sequence. See complete history. | ||
| Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
| Annotation program | Chordata Protein Annotation Program | |
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM
