UniProtKB - Q12918 (KLRB1_HUMAN)
Protein
Killer cell lectin-like receptor subfamily B member 1
Gene
KLRB1
Organism
Homo sapiens (Human)
Status
Functioni
Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.3 Publications
GO - Molecular functioni
- carbohydrate binding Source: ProtInc
- transmembrane signaling receptor activity Source: ProtInc
GO - Biological processi
- cell surface receptor signaling pathway Source: ProtInc
- regulation of immune response Source: Reactome
Keywordsi
Molecular function | Receptor |
Ligand | Lectin |
Enzyme and pathway databases
PathwayCommonsi | Q12918 |
Reactomei | R-HSA-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell |
Names & Taxonomyi
Protein namesi | Recommended name: Killer cell lectin-like receptor subfamily B member 1Alternative name(s): C-type lectin domain family 5 member B HNKR-P1a Short name: NKR-P1A Natural killer cell surface protein P1A CD_antigen: CD161 |
Gene namesi | Name:KLRB1 Synonyms:CLEC5B, NKRP1A |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6373, KLRB1 |
MIMi | 602890, gene |
neXtProti | NX_Q12918 |
VEuPathDBi | HostDB:ENSG00000111796.3 |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass type II membrane protein Curated
Plasma Membrane
- plasma membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 45 | CytoplasmicSequence analysisAdd BLAST | 45 | |
Transmembranei | 46 – 66 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 67 – 225 | ExtracellularSequence analysisAdd BLAST | 159 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 3820 |
OpenTargetsi | ENSG00000111796 |
PharmGKBi | PA30162 |
Miscellaneous databases
Pharosi | Q12918, Tbio |
Genetic variation databases
BioMutai | KLRB1 |
DMDMi | 74722301 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000260000 | 1 – 225 | Killer cell lectin-like receptor subfamily B member 1Add BLAST | 225 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 94 ↔ 105 | PROSITE-ProRule annotation | ||
Disulfide bondi | 122 ↔ 210 | PROSITE-ProRule annotation | ||
Glycosylationi | 157 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 189 ↔ 202 | PROSITE-ProRule annotation |
Post-translational modificationi
N-glycosylated. Contains sialic acid residues.1 Publication
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MassIVEi | Q12918 |
PaxDbi | Q12918 |
PeptideAtlasi | Q12918 |
PRIDEi | Q12918 |
ProteomicsDBi | 59026 |
PTM databases
GlyGeni | Q12918, 1 site |
Expressioni
Tissue specificityi
Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.2 Publications
Inductioni
By IL12/interleukin-12 in NK cells.1 Publication
Gene expression databases
Bgeei | ENSG00000111796, Expressed in granulocyte and 146 other tissues |
Genevisiblei | Q12918, HS |
Organism-specific databases
HPAi | ENSG00000111796, Tissue enriched (blood) |
Interactioni
Subunit structurei
Homodimer; disulfide-linked.
Interacts with acid sphingomyelinase/SMPD1.
4 PublicationsBinary interactionsi
Q12918
With | #Exp. | IntAct |
---|---|---|
CLEC2D - isoform 1 [Q9UHP7-1] | 2 | EBI-2805465,EBI-13640978 |
Protein-protein interaction databases
IntActi | Q12918, 2 interactors |
STRINGi | 9606.ENSP00000229402 |
Miscellaneous databases
RNActi | Q12918, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q12918 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 101 – 211 | C-type lectinPROSITE-ProRule annotationAdd BLAST | 111 |
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4297, Eukaryota |
GeneTreei | ENSGT00940000154685 |
HOGENOMi | CLU_049894_8_2_1 |
InParanoidi | Q12918 |
OMAi | TEIRWIC |
OrthoDBi | 1341815at2759 |
PhylomeDBi | Q12918 |
TreeFami | TF337735 |
Family and domain databases
CDDi | cd03593, CLECT_NK_receptors_like, 1 hit |
Gene3Di | 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR016187, CTDL_fold IPR033992, NKR-like_CTLD |
Pfami | View protein in Pfam PF00059, Lectin_C, 1 hit |
SMARTi | View protein in SMART SM00034, CLECT, 1 hit |
SUPFAMi | SSF56436, SSF56436, 1 hit |
PROSITEi | View protein in PROSITE PS50041, C_TYPE_LECTIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12918-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDQQAIYAEL NLPTDSGPES SSPSSLPRDV CQGSPWHQFA LKLSCAGIIL
60 70 80 90 100
LVLVVTGLSV SVTSLIQKSS IEKCSVDIQQ SRNKTTERPG LLNCPIYWQQ
110 120 130 140 150
LREKCLLFSH TVNPWNNSLA DCSTKESSLL LIRDKDELIH TQNLIRDKAI
160 170 180 190 200
LFWIGLNFSL SEKNWKWING SFLNSNDLEI RGDAKENSCI SISQTSVYSE
210 220
YCSTEIRWIC QKELTPVRNK VYPDS
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_028981 | 168 | I → T1 PublicationCorresponds to variant dbSNP:rs1135816Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U11276 mRNA Translation: AAA21605.1 BC113997 mRNA Translation: AAI13998.1 BC114516 mRNA Translation: AAI14517.1 |
CCDSi | CCDS8601.1 |
PIRi | I38700 |
RefSeqi | NP_002249.1, NM_002258.2 |
Genome annotation databases
Ensembli | ENST00000229402; ENSP00000229402; ENSG00000111796 |
GeneIDi | 3820 |
KEGGi | hsa:3820 |
UCSCi | uc010sgt.3, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - Glycan Binding NKRP1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U11276 mRNA Translation: AAA21605.1 BC113997 mRNA Translation: AAI13998.1 BC114516 mRNA Translation: AAI14517.1 |
CCDSi | CCDS8601.1 |
PIRi | I38700 |
RefSeqi | NP_002249.1, NM_002258.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5MGR | X-ray | 1.80 | A/B | 90-225 | [»] | |
5MGS | X-ray | 1.90 | A/B/C/D/E/F/G/H | 90-225 | [»] | |
5MGT | X-ray | 1.90 | C/D/E/F | 90-225 | [»] | |
SMRi | Q12918 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q12918, 2 interactors |
STRINGi | 9606.ENSP00000229402 |
PTM databases
GlyGeni | Q12918, 1 site |
Genetic variation databases
BioMutai | KLRB1 |
DMDMi | 74722301 |
Proteomic databases
MassIVEi | Q12918 |
PaxDbi | Q12918 |
PeptideAtlasi | Q12918 |
PRIDEi | Q12918 |
ProteomicsDBi | 59026 |
Protocols and materials databases
Antibodypediai | 11606, 913 antibodies |
DNASUi | 3820 |
Genome annotation databases
Ensembli | ENST00000229402; ENSP00000229402; ENSG00000111796 |
GeneIDi | 3820 |
KEGGi | hsa:3820 |
UCSCi | uc010sgt.3, human |
Organism-specific databases
CTDi | 3820 |
DisGeNETi | 3820 |
GeneCardsi | KLRB1 |
HGNCi | HGNC:6373, KLRB1 |
HPAi | ENSG00000111796, Tissue enriched (blood) |
MIMi | 602890, gene |
neXtProti | NX_Q12918 |
OpenTargetsi | ENSG00000111796 |
PharmGKBi | PA30162 |
VEuPathDBi | HostDB:ENSG00000111796.3 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4297, Eukaryota |
GeneTreei | ENSGT00940000154685 |
HOGENOMi | CLU_049894_8_2_1 |
InParanoidi | Q12918 |
OMAi | TEIRWIC |
OrthoDBi | 1341815at2759 |
PhylomeDBi | Q12918 |
TreeFami | TF337735 |
Enzyme and pathway databases
PathwayCommonsi | Q12918 |
Reactomei | R-HSA-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell |
Miscellaneous databases
BioGRID-ORCSi | 3820, 6 hits in 978 CRISPR screens |
GeneWikii | KLRB1 |
GenomeRNAii | 3820 |
Pharosi | Q12918, Tbio |
PROi | PR:Q12918 |
RNActi | Q12918, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000111796, Expressed in granulocyte and 146 other tissues |
Genevisiblei | Q12918, HS |
Family and domain databases
CDDi | cd03593, CLECT_NK_receptors_like, 1 hit |
Gene3Di | 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR016187, CTDL_fold IPR033992, NKR-like_CTLD |
Pfami | View protein in Pfam PF00059, Lectin_C, 1 hit |
SMARTi | View protein in SMART SM00034, CLECT, 1 hit |
SUPFAMi | SSF56436, SSF56436, 1 hit |
PROSITEi | View protein in PROSITE PS50041, C_TYPE_LECTIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KLRB1_HUMAN | |
Accessioni | Q12918Primary (citable) accession number: Q12918 Secondary accession number(s): Q24K24 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 14, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human cell differentiation molecules
CD nomenclature of surface proteins of human leucocytes and list of entries - Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references