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Protein

G-rich sequence factor 1

Gene

GRSF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences.2 Publications

Miscellaneous

Depletion of GRSF1 by siRNA results in a combined OXPHOS assembly defect, with the prominent loss of complexes I, III, IV, and V. It also leads to altered steady-state levels of mitochondrial rRNAs and mRNAs.

GO - Molecular functioni

  • mRNA binding Source: ProtInc
  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, tRNA processing

Enzyme and pathway databases

ReactomeiR-HSA-192823 Viral mRNA Translation

Names & Taxonomyi

Protein namesi
Recommended name:
G-rich sequence factor 1
Short name:
GRSF-1
Gene namesi
Name:GRSF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000132463.13
HGNCiHGNC:4610 GRSF1
MIMi604851 gene
neXtProtiNX_Q12849

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Mitochondrion nucleoid

Pathology & Biotechi

Organism-specific databases

DisGeNETi2926
OpenTargetsiENSG00000132463
PharmGKBiPA29003

Polymorphism and mutation databases

BioMutaiGRSF1
DMDMi215274142

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 117MitochondrionSequence analysisAdd BLAST117
ChainiPRO_0000081597118 – 480G-rich sequence factor 1Add BLAST363

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei244PhosphoserineCombined sources1
Modified residuei335PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ12849
MaxQBiQ12849
PaxDbiQ12849
PeptideAtlasiQ12849
PRIDEiQ12849
ProteomicsDBi58982
58983 [Q12849-5]

PTM databases

iPTMnetiQ12849
PhosphoSitePlusiQ12849

Expressioni

Gene expression databases

BgeeiENSG00000132463 Expressed in 235 organ(s), highest expression level in female gonad
CleanExiHS_GRSF1
ExpressionAtlasiQ12849 baseline and differential
GenevisibleiQ12849 HS

Organism-specific databases

HPAiHPA036984
HPA036985

Interactioni

Subunit structurei

Monomer. Found in a complex with DDX28, DHX30, FASTKD2 and FASTKD5 (PubMed:25683715). Interacts with the mitochondrial RNase P complex subunit TRMT10C/MRPP1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PSMD10O758324EBI-1054150,EBI-752185

Protein-protein interaction databases

BioGridi109182, 90 interactors
CORUMiQ12849
IntActiQ12849, 12 interactors
STRINGi9606.ENSP00000254799

Structurei

Secondary structure

1480
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ12849
SMRiQ12849
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini122 – 246RRM 1PROSITE-ProRule annotationAdd BLAST125
Domaini250 – 326RRM 2PROSITE-ProRule annotationAdd BLAST77
Domaini401 – 480RRM 3PROSITE-ProRule annotationAdd BLAST80

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 111Ala-richAdd BLAST57

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiENOG410IQ3R Eukaryota
ENOG410YEKV LUCA
GeneTreeiENSGT00760000119102
HOGENOMiHOG000220896
HOVERGENiHBG055557
InParanoidiQ12849
OrthoDBiEOG091G0CTJ
PhylomeDBiQ12849
TreeFamiTF316157

Family and domain databases

CDDicd12730 RRM1_GRSF1, 1 hit
cd12505 RRM2_GRSF1, 1 hit
cd12733 RRM3_GRSF1, 1 hit
Gene3Di3.30.70.330, 3 hits
InterProiView protein in InterPro
IPR034424 GRSF-1_RRM2
IPR033106 GRSF1
IPR034425 GRSF1_RRM1
IPR034426 GRSF1_RRM3
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR13976:SF42 PTHR13976:SF42, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 3 hits
SUPFAMiSSF54928 SSF54928, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 3 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q12849-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAGTRWVLGA LLRGCGCNCS SCRRTGAACL PFYSAAGSIP SGVSGRRRLL
60 70 80 90 100
LLLGAAAAAA SQTRGLQTGP VPPGRLAGPP AVATSAAAAA AASYSALRAS
110 120 130 140 150
LLPQSLAAAA AVPTRSYSQE SKTTYLEDLP PPPEYELAPS KLEEEVDDVF
160 170 180 190 200
LIRAQGLPWS CTMEDVLNFF SDCRIRNGEN GIHFLLNRDG KRRGDALIEM
210 220 230 240 250
ESEQDVQKAL EKHRMYMGQR YVEVYEINNE DVDALMKSLQ VKSSPVVNDG
260 270 280 290 300
VVRLRGLPYS CNEKDIVDFF AGLNIVDITF VMDYRGRRKT GEAYVQFEEP
310 320 330 340 350
EMANQALLKH REEIGNRYIE IFPSRRNEVR THVGSYKGKK IASFPTAKYI
360 370 380 390 400
TEPEMVFEEH EVNEDIQPMT AFESEKEIEL PKEVPEKLPE AADFGTTSSL
410 420 430 440 450
HFVHMRGLPF QANAQDIINF FAPLKPVRIT MEYSSSGKAT GEADVHFETH
460 470 480
EDAVAAMLKD RSHVHHRYIE LFLNSCPKGK
Length:480
Mass (Da):53,126
Last modified:November 25, 2008 - v3
Checksum:iE09EE4E70212A70F
GO
Isoform 2 (identifier: Q12849-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-162: Missing.

Note: No experimental confirmation available.
Show »
Length:318
Mass (Da):36,613
Checksum:iF0AB13A4797FFEBD
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y8R1H0Y8R1_HUMAN
G-rich sequence factor 1
GRSF1
417Annotation score:
H0YAK1H0YAK1_HUMAN
G-rich sequence factor 1
GRSF1
453Annotation score:
F5H5I6F5H5I6_HUMAN
G-rich sequence factor 1
GRSF1
424Annotation score:
H0YAM1H0YAM1_HUMAN
G-rich sequence factor 1
GRSF1
114Annotation score:

Sequence cautioni

The sequence AAC95399 differs from that shown. Unlikely isoform. Aberrant splice sites.Curated
The sequence AAH40485 differs from that shown. Intron retention.Curated
The sequence AAY40942 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAC03513 differs from that shown. Unlikely isoform. Aberrant splice sites.Curated
The sequence BAC86863 differs from that shown. Unlikely isoform. Aberrant splice sites.Curated
The sequence EAX05634 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti264K → E in BAC86863 (PubMed:14702039).Curated1
Sequence conflicti365 – 366DI → VF in AAC95399 (PubMed:8036161).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_047537277D → Y1 PublicationCorresponds to variant dbSNP:rs17854012Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0431181 – 162Missing in isoform 2. 1 PublicationAdd BLAST162

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07231 mRNA Translation: AAC95399.1 Sequence problems.
AK056891 mRNA Translation: BAG51822.1
AK090755 mRNA Translation: BAC03513.1 Sequence problems.
AK094806 mRNA Translation: BAG52934.1
AK095799 mRNA Translation: BAG53131.1
AK097055 mRNA Translation: BAG53414.1
AK127161 mRNA Translation: BAC86863.1 Sequence problems.
AC021989 Genomic DNA Translation: AAY40942.1 Sequence problems.
CH471057 Genomic DNA Translation: EAX05631.1
CH471057 Genomic DNA Translation: EAX05634.1 Sequence problems.
BC040485 mRNA Translation: AAH40485.1 Sequence problems.
CCDSiCCDS47069.1 [Q12849-1]
CCDS47070.1 [Q12849-5]
PIRiS48081
RefSeqiNP_001091947.1, NM_001098477.1 [Q12849-5]
NP_002083.3, NM_002092.3
XP_011530199.1, XM_011531897.2 [Q12849-5]
UniGeneiHs.309763

Genome annotation databases

EnsembliENST00000254799; ENSP00000254799; ENSG00000132463 [Q12849-1]
ENST00000502323; ENSP00000425430; ENSG00000132463 [Q12849-5]
GeneIDi2926
KEGGihsa:2926
UCSCiuc010iia.2 human [Q12849-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07231 mRNA Translation: AAC95399.1 Sequence problems.
AK056891 mRNA Translation: BAG51822.1
AK090755 mRNA Translation: BAC03513.1 Sequence problems.
AK094806 mRNA Translation: BAG52934.1
AK095799 mRNA Translation: BAG53131.1
AK097055 mRNA Translation: BAG53414.1
AK127161 mRNA Translation: BAC86863.1 Sequence problems.
AC021989 Genomic DNA Translation: AAY40942.1 Sequence problems.
CH471057 Genomic DNA Translation: EAX05631.1
CH471057 Genomic DNA Translation: EAX05634.1 Sequence problems.
BC040485 mRNA Translation: AAH40485.1 Sequence problems.
CCDSiCCDS47069.1 [Q12849-1]
CCDS47070.1 [Q12849-5]
PIRiS48081
RefSeqiNP_001091947.1, NM_001098477.1 [Q12849-5]
NP_002083.3, NM_002092.3
XP_011530199.1, XM_011531897.2 [Q12849-5]
UniGeneiHs.309763

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LMINMR-A140-245[»]
4QU6X-ray1.75A140-245[»]
4QU7X-ray2.50A/B/C/D400-480[»]
ProteinModelPortaliQ12849
SMRiQ12849
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109182, 90 interactors
CORUMiQ12849
IntActiQ12849, 12 interactors
STRINGi9606.ENSP00000254799

PTM databases

iPTMnetiQ12849
PhosphoSitePlusiQ12849

Polymorphism and mutation databases

BioMutaiGRSF1
DMDMi215274142

Proteomic databases

EPDiQ12849
MaxQBiQ12849
PaxDbiQ12849
PeptideAtlasiQ12849
PRIDEiQ12849
ProteomicsDBi58982
58983 [Q12849-5]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254799; ENSP00000254799; ENSG00000132463 [Q12849-1]
ENST00000502323; ENSP00000425430; ENSG00000132463 [Q12849-5]
GeneIDi2926
KEGGihsa:2926
UCSCiuc010iia.2 human [Q12849-1]

Organism-specific databases

CTDi2926
DisGeNETi2926
EuPathDBiHostDB:ENSG00000132463.13
GeneCardsiGRSF1
HGNCiHGNC:4610 GRSF1
HPAiHPA036984
HPA036985
MIMi604851 gene
neXtProtiNX_Q12849
OpenTargetsiENSG00000132463
PharmGKBiPA29003
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IQ3R Eukaryota
ENOG410YEKV LUCA
GeneTreeiENSGT00760000119102
HOGENOMiHOG000220896
HOVERGENiHBG055557
InParanoidiQ12849
OrthoDBiEOG091G0CTJ
PhylomeDBiQ12849
TreeFamiTF316157

Enzyme and pathway databases

ReactomeiR-HSA-192823 Viral mRNA Translation

Miscellaneous databases

ChiTaRSiGRSF1 human
GenomeRNAii2926
PROiPR:Q12849
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132463 Expressed in 235 organ(s), highest expression level in female gonad
CleanExiHS_GRSF1
ExpressionAtlasiQ12849 baseline and differential
GenevisibleiQ12849 HS

Family and domain databases

CDDicd12730 RRM1_GRSF1, 1 hit
cd12505 RRM2_GRSF1, 1 hit
cd12733 RRM3_GRSF1, 1 hit
Gene3Di3.30.70.330, 3 hits
InterProiView protein in InterPro
IPR034424 GRSF-1_RRM2
IPR033106 GRSF1
IPR034425 GRSF1_RRM1
IPR034426 GRSF1_RRM3
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR13976:SF42 PTHR13976:SF42, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 3 hits
SUPFAMiSSF54928 SSF54928, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGRSF1_HUMAN
AccessioniPrimary (citable) accession number: Q12849
Secondary accession number(s): B3KPW0
, Q4W5S5, Q6ZST3, Q8IWD6, Q8NBD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 25, 2008
Last modified: November 7, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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