UniProtKB - Q12837 (PO4F2_HUMAN)
POU domain, class 4, transcription factor 2
POU4F2
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 345 – 404 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: ParkinsonsUK-UCL
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: UniProtKB
- p53 binding Source: Ensembl
- promoter-specific chromatin binding Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- axon extension Source: Ensembl
- axon guidance Source: UniProtKB
- cell differentiation Source: GO_Central
- cellular response to cytokine stimulus Source: UniProtKB
- cellular response to estradiol stimulus Source: UniProtKB
- cellular response to insulin stimulus Source: Ensembl
- cellular response to oxygen levels Source: Ensembl
- dorsal root ganglion development Source: UniProtKB
- heart development Source: ARUK-UCL
- intracellular estrogen receptor signaling pathway Source: UniProtKB
- intrinsic apoptotic signaling pathway by p53 class mediator Source: UniProtKB
- MAPK cascade Source: UniProtKB
- negative regulation of adipose tissue development Source: Ensembl
- negative regulation of amacrine cell differentiation Source: UniProtKB
- negative regulation of cell differentiation Source: UniProtKB
- negative regulation of DNA-binding transcription factor activity Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: ParkinsonsUK-UCL
- neuromuscular process controlling balance Source: Ensembl
- neuron differentiation Source: UniProtKB
- positive regulation of axon extension Source: UniProtKB
- positive regulation of cardiac muscle cell apoptotic process Source: Ensembl
- positive regulation of cell differentiation Source: UniProtKB
- positive regulation of glucose import Source: Ensembl
- positive regulation of osteoclast differentiation Source: UniProtKB
- positive regulation of programmed cell death Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: ParkinsonsUK-UCL
- positive regulation of transcription regulatory region DNA binding Source: UniProtKB
- regulation of DNA-binding transcription factor activity Source: UniProtKB
- regulation of retinal ganglion cell axon guidance Source: UniProtKB
- regulation of signal transduction by p53 class mediator Source: Reactome
- retina development in camera-type eye Source: UniProtKB
- retinal ganglion cell axon guidance Source: Ensembl
- sensory perception of sound Source: Ensembl
- spermatogenesis Source: Ensembl
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding, Repressor |
Biological process | Apoptosis, Differentiation, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q12837 |
Reactomei | R-HSA-6804759, Regulation of TP53 Activity through Association with Co-factors |
SignaLinki | Q12837 |
SIGNORi | Q12837 |
Names & Taxonomyi
Protein namesi | Recommended name: POU domain, class 4, transcription factor 2CuratedAlternative name(s): Brain-specific homeobox/POU domain protein 3B Short name: Brain-3B Short name: Brn-3B |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000151615.3 |
HGNCi | HGNC:9219, POU4F2 |
MIMi | 113725, gene |
neXtProti | NX_Q12837 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
- Nucleus speckle 1 Publication
Other locations
- Cytoplasm By similarity
Nucleus
- nuclear chromatin Source: NTNU_SB
- nuclear euchromatin Source: Ensembl
- nuclear speck Source: UniProtKB
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- transcription regulator complex Source: ARUK-UCL
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 171 – 185 | Missing : Absent from nuclear speckle; no change in transcriptional activity. 1 PublicationAdd BLAST | 15 |
Organism-specific databases
DisGeNETi | 5458 |
OpenTargetsi | ENSG00000151615 |
PharmGKBi | PA33543 |
Miscellaneous databases
Pharosi | Q12837, Tbio |
Polymorphism and mutation databases
BioMutai | POU4F2 |
DMDMi | 290457652 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100740 | 1 – 409 | POU domain, class 4, transcription factor 2Add BLAST | 409 |
Proteomic databases
MassIVEi | Q12837 |
PaxDbi | Q12837 |
PeptideAtlasi | Q12837 |
PRIDEi | Q12837 |
ProteomicsDBi | 58978 |
PTM databases
iPTMneti | Q12837 |
PhosphoSitePlusi | Q12837 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000151615, Expressed in secondary oocyte and 30 other tissues |
Genevisiblei | Q12837, HS |
Organism-specific databases
HPAi | ENSG00000151615, Group enriched (brain, testis) |
Interactioni
Subunit structurei
Interacts with POU4F1; this interaction inhibits both POU4F1 DNA-binding and transcriptional activities.
Interacts (C-terminus) with ESR1 (via DNA-binding domain); this interaction increases the estrogen receptor ESR1 transcriptional activity in a DNA- and ligand 17-beta-estradiol-independent manner.
Interacts (via C-terminus) with TP53 (via N-terminus).
Interacts with DLX1 (via homeobox DNA-binding domain); this interaction suppresses DLX1-mediated transcriptional activity in postnatal retina enhancing retinal ganglion cell (RGC) differentiation.
Interacts with DLX2 (via homeobox DNA-binding domain); this interaction enhances RGC differentiation.
Interacts (via C-terminus) with ISL1 (via C-terminus).
Interacts with ISL2.
Interacts with LHX2.
By similarityBinary interactionsi
Q12837
GO - Molecular functioni
- p53 binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 111454, 75 interactors |
IntActi | Q12837, 71 interactors |
STRINGi | 9606.ENSP00000281321 |
Miscellaneous databases
RNActi | Q12837, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 250 – 327 | POU-specificPROSITE-ProRule annotationAdd BLAST | 78 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 91 – 237 | Required for transcriptional activationBy similarityAdd BLAST | 147 | |
Regioni | 238 – 409 | Required for DNA-binding and transcriptional repressionBy similarityAdd BLAST | 172 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 110 – 119 | POU-IV box | 10 | |
Motifi | 171 – 185 | Nuclear speckle targeting signal1 PublicationAdd BLAST | 15 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 27 – 79 | Ser-richPROSITE-ProRule annotationAdd BLAST | 53 | |
Compositional biasi | 54 – 83 | Gly-richPROSITE-ProRule annotationAdd BLAST | 30 | |
Compositional biasi | 126 – 185 | His-richPROSITE-ProRule annotationAdd BLAST | 60 |
Domaini
Sequence similaritiesi
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG1168, Eukaryota |
GeneTreei | ENSGT00940000160339 |
HOGENOMi | CLU_013065_0_0_1 |
InParanoidi | Q12837 |
OMAi | THAPHMA |
OrthoDBi | 929123at2759 |
PhylomeDBi | Q12837 |
TreeFami | TF316413 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit cd00093, HTH_XRE, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR001387, Cro/C1-type_HTH IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMMMSLNSKQ AFSMPHGGSL HVEPKYSALH STSPGSSAPI APSASSPSSS
60 70 80 90 100
SNAGGGGGGG GGGGGGGGRS SSSSSSGSSG GGGSEAMRRA CLPTPPSNIF
110 120 130 140 150
GGLDESLLAR AEALAAVDIV SQSKSHHHHP PHHSPFKPDA TYHTMNTIPC
160 170 180 190 200
TSAASSSSVP ISHPSALAGT HHHHHHHHHH HHQPHQALEG ELLEHLSPGL
210 220 230 240 250
ALGAMAGPDG AVVSTPAHAP HMATMNPMHQ AALSMAHAHG LPSHMGCMSD
260 270 280 290 300
VDADPRDLEA FAERFKQRRI KLGVTQADVG SALANLKIPG VGSLSQSTIC
310 320 330 340 350
RFESLTLSHN NMIALKPILQ AWLEEAEKSH REKLTKPELF NGAEKKRKRT
360 370 380 390 400
SIAAPEKRSL EAYFAIQPRP SSEKIAAIAE KLDLKKNVVR VWFCNQRQKQ
KRMKYSAGI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 54 | G → GG in AAA16509 (PubMed:7691107).Curated | 1 | |
Sequence conflicti | 54 | G → GG in BAG37481 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 54 | G → GG in AAI36345 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 54 | G → GG in AAI36346 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 165 | S → C in AAA16509 (PubMed:7691107).Curated | 1 | |
Sequence conflicti | 182 | Missing in CAA50589 (PubMed:8332509).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_059321 | 40 | I → T. Corresponds to variant dbSNP:rs13152799Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_058837 | 1 – 144 | Missing in isoform 2. Add BLAST | 144 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U06233 mRNA Translation: AAA16509.1 AK314982 mRNA Translation: BAG37481.1 AC093887 Genomic DNA No translation available. BC136344 mRNA Translation: AAI36345.1 BC136345 mRNA Translation: AAI36346.1 EU439706 mRNA Translation: ACA49233.1 X71488 mRNA Translation: CAA50589.1 L20434 mRNA Translation: AAA36393.1 |
CCDSi | CCDS34074.1 [Q12837-1] |
PIRi | I38502 |
RefSeqi | NP_004566.2, NM_004575.2 [Q12837-1] |
Genome annotation databases
Ensembli | ENST00000281321; ENSP00000281321; ENSG00000151615 [Q12837-1] |
GeneIDi | 5458 |
KEGGi | hsa:5458 |
UCSCi | uc003ikv.4, human [Q12837-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U06233 mRNA Translation: AAA16509.1 AK314982 mRNA Translation: BAG37481.1 AC093887 Genomic DNA No translation available. BC136344 mRNA Translation: AAI36345.1 BC136345 mRNA Translation: AAI36346.1 EU439706 mRNA Translation: ACA49233.1 X71488 mRNA Translation: CAA50589.1 L20434 mRNA Translation: AAA36393.1 |
CCDSi | CCDS34074.1 [Q12837-1] |
PIRi | I38502 |
RefSeqi | NP_004566.2, NM_004575.2 [Q12837-1] |
3D structure databases
SMRi | Q12837 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 111454, 75 interactors |
IntActi | Q12837, 71 interactors |
STRINGi | 9606.ENSP00000281321 |
PTM databases
iPTMneti | Q12837 |
PhosphoSitePlusi | Q12837 |
Polymorphism and mutation databases
BioMutai | POU4F2 |
DMDMi | 290457652 |
Proteomic databases
MassIVEi | Q12837 |
PaxDbi | Q12837 |
PeptideAtlasi | Q12837 |
PRIDEi | Q12837 |
ProteomicsDBi | 58978 |
Protocols and materials databases
Antibodypediai | 16443, 165 antibodies |
Genome annotation databases
Ensembli | ENST00000281321; ENSP00000281321; ENSG00000151615 [Q12837-1] |
GeneIDi | 5458 |
KEGGi | hsa:5458 |
UCSCi | uc003ikv.4, human [Q12837-1] |
Organism-specific databases
CTDi | 5458 |
DisGeNETi | 5458 |
EuPathDBi | HostDB:ENSG00000151615.3 |
GeneCardsi | POU4F2 |
HGNCi | HGNC:9219, POU4F2 |
HPAi | ENSG00000151615, Group enriched (brain, testis) |
MIMi | 113725, gene |
neXtProti | NX_Q12837 |
OpenTargetsi | ENSG00000151615 |
PharmGKBi | PA33543 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1168, Eukaryota |
GeneTreei | ENSGT00940000160339 |
HOGENOMi | CLU_013065_0_0_1 |
InParanoidi | Q12837 |
OMAi | THAPHMA |
OrthoDBi | 929123at2759 |
PhylomeDBi | Q12837 |
TreeFami | TF316413 |
Enzyme and pathway databases
PathwayCommonsi | Q12837 |
Reactomei | R-HSA-6804759, Regulation of TP53 Activity through Association with Co-factors |
SignaLinki | Q12837 |
SIGNORi | Q12837 |
Miscellaneous databases
BioGRID-ORCSi | 5458, 3 hits in 865 CRISPR screens |
GeneWikii | POU4F2 |
GenomeRNAii | 5458 |
Pharosi | Q12837, Tbio |
PROi | PR:Q12837 |
RNActi | Q12837, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000151615, Expressed in secondary oocyte and 30 other tissues |
Genevisiblei | Q12837, HS |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit cd00093, HTH_XRE, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR001387, Cro/C1-type_HTH IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PO4F2_HUMAN | |
Accessioni | Q12837Primary (citable) accession number: Q12837 Secondary accession number(s): B1PJR6 Q14987 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | March 2, 2010 | |
Last modified: | December 2, 2020 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations