UniProtKB - Q12836 (ZP4_HUMAN)
Protein
Zona pellucida sperm-binding protein 4
Gene
ZP4
Organism
Homo sapiens (Human)
Status
Functioni
The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.
GO - Molecular functioni
- acrosin binding Source: UniProtKB
- identical protein binding Source: IntAct
GO - Biological processi
- acrosomal vesicle exocytosis Source: UniProtKB
- binding of sperm to zona pellucida Source: Reactome
- negative regulation of binding of sperm to zona pellucida Source: UniProtKB
- positive regulation of acrosome reaction Source: UniProtKB
- positive regulation of humoral immune response Source: UniProtKB
- positive regulation of T cell proliferation Source: UniProtKB
Keywordsi
Molecular function | Receptor |
Biological process | Fertilization |
Enzyme and pathway databases
Reactomei | R-HSA-1300644 Interaction With The Zona Pellucida |
Names & Taxonomyi
Protein namesi | Recommended name: Zona pellucida sperm-binding protein 4Alternative name(s): Zona pellucida glycoprotein 4 Short name: Zp-4 Zona pellucida protein B Cleaved into the following chain: |
Gene namesi | Name:ZP4 Synonyms:ZPB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000116996.9 |
HGNCi | HGNC:15770 ZP4 |
MIMi | 613514 gene |
neXtProti | NX_Q12836 |
Subcellular locationi
Processed zona pellucida sperm-binding protein 4 :
Extracellular region or secreted
- extracellular matrix By similarity
Note: The glycoproteinaceous translucent extracellular matrix that surrounds the mammalian oocyte is called zona pellucida.Curated
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
Extracellular region or secreted
- extracellular region Source: Reactome
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 19 – 505 | ExtracellularSequence analysisAdd BLAST | 487 | |
Transmembranei | 506 – 526 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 527 – 540 | CytoplasmicSequence analysisAdd BLAST | 14 |
Keywords - Cellular componenti
Cell membrane, Extracellular matrix, Membrane, SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 57829 |
OpenTargetsi | ENSG00000116996 |
PharmGKBi | PA38036 |
Polymorphism and mutation databases
BioMutai | ZP4 |
DMDMi | 46397080 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000041727 | 19 – 462 | Zona pellucida sperm-binding protein 4Add BLAST | 444 | |
ChainiPRO_0000304578 | 19 – ? | Processed zona pellucida sperm-binding protein 4 | ||
PropeptideiPRO_0000041728 | 463 – 540 | Removed in mature formBy similarityAdd BLAST | 78 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 69 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 202 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Glycosylationi | 219 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Glycosylationi | 267 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 302 | O-linked (GalNAc...) threonineBy similarity | 1 | |
Disulfide bondi | 367 ↔ 442 | PROSITE-ProRule annotation | ||
Glycosylationi | 470 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 474 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q12836 |
PeptideAtlasi | Q12836 |
PRIDEi | Q12836 |
ProteomicsDBi | 58977 |
PTM databases
iPTMneti | Q12836 |
PhosphoSitePlusi | Q12836 |
Expressioni
Tissue specificityi
Oocytes.
Gene expression databases
Bgeei | ENSG00000116996 Expressed in 8 organ(s), highest expression level in oocyte |
Genevisiblei | Q12836 HS |
Organism-specific databases
HPAi | HPA008547 |
Interactioni
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
itself | 6 | EBI-11783805,EBI-11783805 |
GO - Molecular functioni
- acrosin binding Source: UniProtKB
- identical protein binding Source: IntAct
Protein-protein interaction databases
MINTi | Q12836 |
STRINGi | 9606.ENSP00000355529 |
Structurei
3D structure databases
ProteinModelPortali | Q12836 |
SMRi | Q12836 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 141 – 183 | P-typePROSITE-ProRule annotationAdd BLAST | 43 | |
Domaini | 188 – 466 | ZPPROSITE-ProRule annotationAdd BLAST | 279 |
Domaini
The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG410IE4J Eukaryota ENOG4111H75 LUCA |
GeneTreei | ENSGT00940000161324 |
HOGENOMi | HOG000059607 |
HOVERGENi | HBG050410 |
InParanoidi | Q12836 |
OMAi | SCYYGNT |
OrthoDBi | 586615at2759 |
PhylomeDBi | Q12836 |
TreeFami | TF332794 |
Family and domain databases
CDDi | cd00111 Trefoil, 1 hit |
InterProi | View protein in InterPro IPR017957 P_trefoil_CS IPR000519 P_trefoil_dom IPR001507 ZP_dom IPR017977 ZP_dom_CS |
Pfami | View protein in Pfam PF00088 Trefoil, 1 hit PF00100 Zona_pellucida, 1 hit |
PRINTSi | PR00023 ZPELLUCIDA |
SMARTi | View protein in SMART SM00018 PD, 1 hit SM00241 ZP, 1 hit |
PROSITEi | View protein in PROSITE PS00025 P_TREFOIL_1, 1 hit PS51448 P_TREFOIL_2, 1 hit PS00682 ZP_1, 1 hit PS51034 ZP_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q12836-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MWLLRCVLLC VSLSLAVSGQ HKPEAPDYSS VLHCGPWSFQ FAVNLNQEAT
60 70 80 90 100
SPPVLIAWDN QGLLHELQND SDCGTWIRKG PGSSVVLEAT YSSCYVTEWD
110 120 130 140 150
SHYIMPVGVE GAGAAEHKVV TERKLLKCPM DLLARDAPDT DWCDSIPARD
160 170 180 190 200
RLPCAPSPIS RGDCEGLGCC YSSEEVNSCY YGNTVTLHCT REGHFSIAVS
210 220 230 240 250
RNVTSPPLLL DSVRLALRND SACNPVMATQ AFVLFQFPFT SCGTTRQITG
260 270 280 290 300
DRAVYENELV ATRDVKNGSR GSVTRDSIFR LHVSCSYSVS SNSLPINVQV
310 320 330 340 350
FTLPPPFPET QPGPLTLELQ IAKDKNYGSY YGVGDYPVVK LLRDPIYVEV
360 370 380 390 400
SILHRTDPYL GLLLQQCWAT PSTDPLSQPQ WPILVKGCPY IGDNYQTQLI
410 420 430 440 450
PVQKALDLPF PSHHQRFSIF TFSFVNPTVE KQALRGPVHL HCSVSVCQPA
460 470 480 490 500
ETPSCVVTCP DLSRRRNFDN SSQNTTASVS SKGPMILLQA TKDPPEKLRV
510 520 530 540
PVDSKVLWVA GLSGTLILGA LLVSYLAVKK QKSCPDQMCQ
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052997 | 114 | A → V. Corresponds to variant dbSNP:rs34370253Ensembl. | 1 | |
Natural variantiVAR_052998 | 295 | P → S. Corresponds to variant dbSNP:rs34811980Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U05781 Genomic DNA Translation: AAA74391.1 AK314151 mRNA Translation: BAG36838.1 AL359924 Genomic DNA No translation available. CH471098 Genomic DNA Translation: EAW70072.1 BC069521 mRNA Translation: AAH69521.1 |
CCDSi | CCDS1615.1 |
PIRi | S70403 |
RefSeqi | NP_067009.1, NM_021186.4 |
UniGenei | Hs.136241 |
Genome annotation databases
Ensembli | ENST00000366570; ENSP00000355529; ENSG00000116996 ENST00000611898; ENSP00000482304; ENSG00000116996 |
GeneIDi | 57829 |
KEGGi | hsa:57829 |
UCSCi | uc001hym.3 human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U05781 Genomic DNA Translation: AAA74391.1 AK314151 mRNA Translation: BAG36838.1 AL359924 Genomic DNA No translation available. CH471098 Genomic DNA Translation: EAW70072.1 BC069521 mRNA Translation: AAH69521.1 |
CCDSi | CCDS1615.1 |
PIRi | S70403 |
RefSeqi | NP_067009.1, NM_021186.4 |
UniGenei | Hs.136241 |
3D structure databases
ProteinModelPortali | Q12836 |
SMRi | Q12836 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
MINTi | Q12836 |
STRINGi | 9606.ENSP00000355529 |
PTM databases
iPTMneti | Q12836 |
PhosphoSitePlusi | Q12836 |
Polymorphism and mutation databases
BioMutai | ZP4 |
DMDMi | 46397080 |
Proteomic databases
PaxDbi | Q12836 |
PeptideAtlasi | Q12836 |
PRIDEi | Q12836 |
ProteomicsDBi | 58977 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENST00000366570; ENSP00000355529; ENSG00000116996 ENST00000611898; ENSP00000482304; ENSG00000116996 |
GeneIDi | 57829 |
KEGGi | hsa:57829 |
UCSCi | uc001hym.3 human |
Organism-specific databases
CTDi | 57829 |
DisGeNETi | 57829 |
EuPathDBi | HostDB:ENSG00000116996.9 |
GeneCardsi | ZP4 |
HGNCi | HGNC:15770 ZP4 |
HPAi | HPA008547 |
MIMi | 613514 gene |
neXtProti | NX_Q12836 |
OpenTargetsi | ENSG00000116996 |
PharmGKBi | PA38036 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG410IE4J Eukaryota ENOG4111H75 LUCA |
GeneTreei | ENSGT00940000161324 |
HOGENOMi | HOG000059607 |
HOVERGENi | HBG050410 |
InParanoidi | Q12836 |
OMAi | SCYYGNT |
OrthoDBi | 586615at2759 |
PhylomeDBi | Q12836 |
TreeFami | TF332794 |
Enzyme and pathway databases
Reactomei | R-HSA-1300644 Interaction With The Zona Pellucida |
Miscellaneous databases
GeneWikii | ZP4 |
GenomeRNAii | 57829 |
PROi | PR:Q12836 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000116996 Expressed in 8 organ(s), highest expression level in oocyte |
Genevisiblei | Q12836 HS |
Family and domain databases
CDDi | cd00111 Trefoil, 1 hit |
InterProi | View protein in InterPro IPR017957 P_trefoil_CS IPR000519 P_trefoil_dom IPR001507 ZP_dom IPR017977 ZP_dom_CS |
Pfami | View protein in Pfam PF00088 Trefoil, 1 hit PF00100 Zona_pellucida, 1 hit |
PRINTSi | PR00023 ZPELLUCIDA |
SMARTi | View protein in SMART SM00018 PD, 1 hit SM00241 ZP, 1 hit |
PROSITEi | View protein in PROSITE PS00025 P_TREFOIL_1, 1 hit PS51448 P_TREFOIL_2, 1 hit PS00682 ZP_1, 1 hit PS51034 ZP_2, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | ZP4_HUMAN | |
Accessioni | Q12836Primary (citable) accession number: Q12836 Secondary accession number(s): B2RAE1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 13, 2019 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations