ZP4

Functionⁱ

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.

GO - Molecular functionⁱ

acrosin binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 15950651
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 26879157

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

acrosomal vesicle exocytosis
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19004505
binding of sperm to zona pellucida Source: Reactome
negative regulation of binding of sperm to zona pellucida
Source: UniProtKB
Inferred from direct assayⁱ
- 18667750
positive regulation of acrosome reaction
Source: UniProtKB
Inferred from direct assayⁱ
- 18667750
- 20394732
positive regulation of humoral immune response
Source: UniProtKB
Inferred from direct assayⁱ
- 19004505
positive regulation of T cell proliferation
Source: UniProtKB
Inferred from direct assayⁱ
- 19004505

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Receptor
Biological process	Fertilization

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1300644 Interaction With The Zona Pellucida

Reactomeⁱ

R-HSA-1300644 Interaction With The Zona Pellucida

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Zona pellucida sperm-binding protein 4 Alternative name(s): Zona pellucida glycoprotein 4 Short name: Zp-4 Zona pellucida protein B Cleaved into the following chain: Processed zona pellucida sperm-binding protein 4
Gene namesⁱ	Name:ZP4 Synonyms:ZPB
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000116996.9
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:15770 ZP4
MIMⁱ	613514 gene
neXtProtⁱ	NX_Q12836

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	19 – 505	ExtracellularSequence analysisAdd BLAST	487
Transmembraneⁱ	506 – 526	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	527 – 540	CytoplasmicSequence analysisAdd BLAST	14

Keywords - Cellular componentⁱ

Cell membrane, Extracellular matrix, Membrane, Secreted

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	57829
Open Targets More...OpenTargetsⁱ	ENSG00000116996
PharmGKBⁱ	PA38036

Polymorphism and mutation databases

BioMutaⁱ	ZP4
DMDMⁱ	46397080

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Signal peptideⁱ	1 – 18	Sequence analysisAdd BLAST	18
ChainⁱPRO_0000041727	19 – 462	Zona pellucida sperm-binding protein 4Add BLAST	444
ChainⁱPRO_0000304578	19 – ?	Processed zona pellucida sperm-binding protein 4
PropeptideⁱPRO_0000041728	463 – 540	Removed in mature formBy similarityAdd BLAST	78

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	69	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	202	N-linked (GlcNAc...) asparagineBy similarity	1
Glycosylationⁱ	219	N-linked (GlcNAc...) asparagineBy similarity	1
Glycosylationⁱ	267	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	302	O-linked (GalNAc...) threonineBy similarity	1
Disulfide bondⁱ	367 ↔ 442	PROSITE-ProRule annotation
Glycosylationⁱ	470	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	474	N-linked (GlcNAc...) asparagineSequence analysis	1

Post-translational modificationⁱ

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.

Keywords - PTMⁱ

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbⁱ	Q12836
PeptideAtlas More...PeptideAtlasⁱ	Q12836
PRIDEⁱ	Q12836
ProteomicsDBⁱ	58977

PTM databases

iPTMnetⁱ	Q12836
PhosphoSitePlusⁱ	Q12836

Expressionⁱ

Tissue specificityⁱ

Oocytes.

Gene expression databases

Bgeeⁱ	ENSG00000116996 Expressed in 8 organ(s), highest expression level in oocyte
CleanExⁱ	HS_ZP4
Genevisibleⁱ	Q12836 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA008547

HPAⁱ

HPA008547

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
itself		6	EBI-11783805,EBI-11783805

With

Entry

#Exp.

IntAct

Notes

itself

6

EBI-11783805,EBI-11783805

GO - Molecular functionⁱ

acrosin binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 15950651
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 26879157

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

Molecular INTeraction database More...MINTⁱ	Q12836
STRINGⁱ	9606.ENSP00000355529

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q12836
SMRⁱ	Q12836
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	141 – 183	P-typePROSITE-ProRule annotationAdd BLAST		43
Domainⁱ	188 – 466	ZPPROSITE-ProRule annotationAdd BLAST		279

Domainⁱ

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Sequence similaritiesⁱ

Belongs to the ZP domain family. ZPB subfamily.Curated

Keywords - Domainⁱ

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IE4J Eukaryota ENOG4111H75 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063096
HOGENOMⁱ	HOG000059607
HOVERGENⁱ	HBG050410
InParanoidⁱ	Q12836
OMAⁱ	SCYYGNT
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0UNU
PhylomeDBⁱ	Q12836
TreeFamⁱ	TF332794

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00111 Trefoil, 1 hit
InterProⁱ	View protein in InterPro IPR017957 P_trefoil_CS IPR000519 P_trefoil_dom IPR001507 ZP_dom IPR017977 ZP_dom_CS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00088 Trefoil, 1 hit PF00100 Zona_pellucida, 1 hit
PRINTSⁱ	PR00023 ZPELLUCIDA
SMARTⁱ	View protein in SMART SM00018 PD, 1 hit SM00241 ZP, 1 hit
PROSITEⁱ	View protein in PROSITE PS00025 P_TREFOIL_1, 1 hit PS51448 P_TREFOIL_2, 1 hit PS00682 ZP_1, 1 hit PS51034 ZP_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q12836-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MWLLRCVLLC VSLSLAVSGQ HKPEAPDYSS VLHCGPWSFQ FAVNLNQEAT 
        60         70         80         90        100
SPPVLIAWDN QGLLHELQND SDCGTWIRKG PGSSVVLEAT YSSCYVTEWD 
       110        120        130        140        150
SHYIMPVGVE GAGAAEHKVV TERKLLKCPM DLLARDAPDT DWCDSIPARD 
       160        170        180        190        200
RLPCAPSPIS RGDCEGLGCC YSSEEVNSCY YGNTVTLHCT REGHFSIAVS 
       210        220        230        240        250
RNVTSPPLLL DSVRLALRND SACNPVMATQ AFVLFQFPFT SCGTTRQITG 
       260        270        280        290        300
DRAVYENELV ATRDVKNGSR GSVTRDSIFR LHVSCSYSVS SNSLPINVQV 
       310        320        330        340        350
FTLPPPFPET QPGPLTLELQ IAKDKNYGSY YGVGDYPVVK LLRDPIYVEV 
       360        370        380        390        400
SILHRTDPYL GLLLQQCWAT PSTDPLSQPQ WPILVKGCPY IGDNYQTQLI 
       410        420        430        440        450
PVQKALDLPF PSHHQRFSIF TFSFVNPTVE KQALRGPVHL HCSVSVCQPA 
       460        470        480        490        500
ETPSCVVTCP DLSRRRNFDN SSQNTTASVS SKGPMILLQA TKDPPEKLRV 
       510        520        530        540
PVDSKVLWVA GLSGTLILGA LLVSYLAVKK QKSCPDQMCQ

Length:540

Mass (Da):59,400

Last modified:November 1, 1996 - v1

Checksum:ⁱ9DAC310D808E1AC3

GO

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_052997	114	A → V. Corresponds to variant dbSNP:rs34370253Ensembl.		1
Natural variantⁱVAR_052998	295	P → S. Corresponds to variant dbSNP:rs34811980Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U05781 Genomic DNA Translation: AAA74391.1 AK314151 mRNA Translation: BAG36838.1 AL359924 Genomic DNA No translation available. CH471098 Genomic DNA Translation: EAW70072.1 BC069521 mRNA Translation: AAH69521.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS1615.1
PIRⁱ	S70403
NCBI Reference Sequences More...RefSeqⁱ	NP_067009.1, NM_021186.4
UniGeneⁱ	Hs.136241

Genome annotation databases

Ensemblⁱ	ENST00000366570; ENSP00000355529; ENSG00000116996 ENST00000611898; ENSP00000482304; ENSG00000116996
GeneIDⁱ	57829
KEGGⁱ	hsa:57829
UCSC genome browser More...UCSCⁱ	uc001hym.3 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q12836	Zona pellucida glycoprotein 4		CHLSB		542	UniRef90_Q12836
ZP4 isoform 1		PANTR		540
ZP4 isoform 1		PONAB		540
Uncharacterized protein		PANPA		540
Uncharacterized protein		MACMU		539
+26

Protein	Similar proteins		Species	Score	Length	Source
Q12836	Zona pellucida sperm-binding protein 4		RABIT		540	UniRef50_Q12836
Zona pellucida sperm-binding protein 4		BOVIN		534
Zona pellucida sperm-binding protein 4		PIG		536
Zona pellucida sperm-binding protein 4		RAT		545
Zona pellucida sperm-binding protein 4		FELCA		570
+148

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U05781 Genomic DNA Translation: AAA74391.1 AK314151 mRNA Translation: BAG36838.1 AL359924 Genomic DNA No translation available. CH471098 Genomic DNA Translation: EAW70072.1 BC069521 mRNA Translation: AAH69521.1
CCDSⁱ	CCDS1615.1
PIRⁱ	S70403
RefSeqⁱ	NP_067009.1, NM_021186.4
UniGeneⁱ	Hs.136241

3D structure databases

ProteinModelPortalⁱ	Q12836
SMRⁱ	Q12836
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

MINTⁱ	Q12836
STRINGⁱ	9606.ENSP00000355529

PTM databases

iPTMnetⁱ	Q12836
PhosphoSitePlusⁱ	Q12836

Polymorphism and mutation databases

BioMutaⁱ	ZP4
DMDMⁱ	46397080

Proteomic databases

PaxDbⁱ	Q12836
PeptideAtlasⁱ	Q12836
PRIDEⁱ	Q12836
ProteomicsDBⁱ	58977

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000366570; ENSP00000355529; ENSG00000116996 ENST00000611898; ENSP00000482304; ENSG00000116996
GeneIDⁱ	57829
KEGGⁱ	hsa:57829
UCSCⁱ	uc001hym.3 human

Organism-specific databases

CTDⁱ	57829
DisGeNETⁱ	57829
EuPathDBⁱ	HostDB:ENSG00000116996.9
GeneCardsⁱ	ZP4
HGNCⁱ	HGNC:15770 ZP4
HPAⁱ	HPA008547
MIMⁱ	613514 gene
neXtProtⁱ	NX_Q12836
OpenTargetsⁱ	ENSG00000116996
PharmGKBⁱ	PA38036
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IE4J Eukaryota ENOG4111H75 LUCA
GeneTreeⁱ	ENSGT00530000063096
HOGENOMⁱ	HOG000059607
HOVERGENⁱ	HBG050410
InParanoidⁱ	Q12836
OMAⁱ	SCYYGNT
OrthoDBⁱ	EOG091G0UNU
PhylomeDBⁱ	Q12836
TreeFamⁱ	TF332794

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1300644 Interaction With The Zona Pellucida

Reactomeⁱ

R-HSA-1300644 Interaction With The Zona Pellucida

Miscellaneous databases

GeneWikiⁱ	ZP4
GenomeRNAiⁱ	57829
Protein Ontology More...PROⁱ	PR:Q12836
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000116996 Expressed in 8 organ(s), highest expression level in oocyte
CleanExⁱ	HS_ZP4
Genevisibleⁱ	Q12836 HS

Family and domain databases

CDDⁱ	cd00111 Trefoil, 1 hit
InterProⁱ	View protein in InterPro IPR017957 P_trefoil_CS IPR000519 P_trefoil_dom IPR001507 ZP_dom IPR017977 ZP_dom_CS
Pfamⁱ	View protein in Pfam PF00088 Trefoil, 1 hit PF00100 Zona_pellucida, 1 hit
PRINTSⁱ	PR00023 ZPELLUCIDA
SMARTⁱ	View protein in SMART SM00018 PD, 1 hit SM00241 ZP, 1 hit
PROSITEⁱ	View protein in PROSITE PS00025 P_TREFOIL_1, 1 hit PS51448 P_TREFOIL_2, 1 hit PS00682 ZP_1, 1 hit PS51034 ZP_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ZP4_HUMAN
Accessionⁱ	Q12836Primary (citable) accession number: Q12836 Secondary accession number(s): B2RAE1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
	Last sequence update:	November 1, 1996
	Last modified:	November 7, 2018
	This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q12836 (ZP4_HUMAN)

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Zona pellucida sperm-binding protein 4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Plasma membrane

Extracellular region or secreted

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ