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UniProtKB - Q12774 (ARHG5_HUMAN)

Entry version 189 (25 May 2022)
Sequence version 3 (02 Mar 2010)
Previous versions | rss
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Protein

Rho guanine nucleotide exchange factor 5

Gene

ARHGEF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Guanine nucleotide exchange factor which activates Rho GTPases (PubMed:15601624).

Strongly activates RHOA (PubMed:15601624).

Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity).

Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:15601624).

Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (PubMed:14662653).

Required for SRC-induced podosome formation (By similarity).

Involved in positive regulation of immature dendritic cell migration (By similarity).

By similarity2 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • GTP binding Source: ProtInc
  • guanyl-nucleotide exchange factor activity Source: UniProtKB
  • lipid binding Source: UniProtKB-KW
Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGuanine-nucleotide releasing factor
LigandLipid-binding

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q12774

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-193648, NRAGE signals death through JNK
R-HSA-416482, G alpha (12/13) signalling events
R-HSA-8980692, RHOA GTPase cycle
R-HSA-9013026, RHOB GTPase cycle
R-HSA-9013106, RHOC GTPase cycle
R-HSA-9013148, CDC42 GTPase cycle
R-HSA-9013149, RAC1 GTPase cycle
R-HSA-9013408, RHOG GTPase cycle

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		Q12774

SIGNOR Signaling Network Open Resource

More...SIGNORi		Q12774

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 5
Alternative name(s):
Ephexin-3
Guanine nucleotide regulatory protein TIM
Oncogene TIM
Transforming immortalized mammary oncogene
p60 TIM
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ARHGEF5
Synonyms:TIM
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 7

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:13209, ARHGEF5

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		600888, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q12774

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000050327

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNET

More...DisGeNETi		7984

Open Targets

More...OpenTargetsi		ENSG00000050327

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA24975

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q12774, Tbio

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		ARHGEF5

Domain mapping of disease mutations (DMDM)

More...DMDMi		290457679

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000809161 – 1597Rho guanine nucleotide exchange factor 5Add BLAST1597

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei184PhosphoserineCombined sources1
Modified residuei445PhosphoserineCombined sources1
Modified residuei450PhosphoserineCombined sources1
Modified residuei866Asymmetric dimethylarginineCombined sources1
Modified residuei983PhosphoserineCombined sources1
Modified residuei1011PhosphoserineCombined sources1
Modified residuei1044PhosphoserineCombined sources1
Modified residuei1126PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Activation of SRC induces tyrosine phosphorylation of ARHGEF5.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q12774

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q12774

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q12774

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q12774

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q12774

PeptideAtlas

More...PeptideAtlasi		Q12774

PRoteomics IDEntifications database

More...PRIDEi		Q12774

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		58920 [Q12774-1]
58921 [Q12774-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q12774

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q12774

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitously expressed with highest levels in placenta. High levels are also found in colon, kidney, trachea, prostate, liver, pancreas, pituitary gland, thyroid gland and mammary gland. In fetal tissues, expressed at high levels in kidney, lung and liver (PubMed:15601624). Expressed at low levels in lung and heart (PubMed:14662653).2 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000050327, Expressed in thoracic mammary gland and 176 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q12774, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q12774, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000050327, Tissue enhanced (skin)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with SRC (By similarity).

Forms a ternary complex with SRC and the PI3K 85 kDa subunit (By similarity).

Interacts with and is activated by the heterodimer formed by GNB1 and GNG2 (By similarity).

Interacts with ODAM (via C-terminus) (PubMed:25911094).

Interacts with RHOA (By similarity).

By similarity1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		113696, 80 interactors

Protein interaction database and analysis system

More...IntActi		Q12774, 39 interactors

Molecular INTeraction database

More...MINTi		Q12774

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000056217

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q12774, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		Q12774

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q12774

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini1174 – 1358DHPROSITE-ProRule annotationAdd BLAST185
Domaini1390 – 1502PHPROSITE-ProRule annotationAdd BLAST113
Domaini1510 – 1571SH3PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni138 – 246DisorderedSequence analysisAdd BLAST109
Regioni258 – 455DisorderedSequence analysisAdd BLAST198
Regioni467 – 1072DisorderedSequence analysisAdd BLAST606

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi176 – 203Polar residuesSequence analysisAdd BLAST28
Compositional biasi262 – 276Basic and acidic residuesSequence analysisAdd BLAST15
Compositional biasi306 – 373Basic and acidic residuesSequence analysisAdd BLAST68
Compositional biasi390 – 404Basic and acidic residuesSequence analysisAdd BLAST15
Compositional biasi429 – 443Polar residuesSequence analysisAdd BLAST15
Compositional biasi473 – 487Basic and acidic residuesSequence analysisAdd BLAST15
Compositional biasi488 – 512Polar residuesSequence analysisAdd BLAST25
Compositional biasi590 – 613Polar residuesSequence analysisAdd BLAST24
Compositional biasi656 – 687Polar residuesSequence analysisAdd BLAST32
Compositional biasi810 – 827Pro residuesSequence analysisAdd BLAST18
Compositional biasi836 – 858Pro residuesSequence analysisAdd BLAST23
Compositional biasi898 – 946Polar residuesSequence analysisAdd BLAST49
Compositional biasi993 – 1014Basic and acidic residuesSequence analysisAdd BLAST22

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The PH domain binds to phosphoinositides and is essential for podosome formation.By similarity

Keywords - Domaini

SH3 domain

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG3523, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT01030000234571

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_003633_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q12774

Identification of Orthologs from Complete Genome Data

More...OMAi		QDDVMLG

Database of Orthologous Groups

More...OrthoDBi		1176939at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q12774

TreeFam database of animal gene trees

More...TreeFami		TF342609

Family and domain databases

Conserved Domains Database

More...CDDi		cd00160, RhoGEF, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.20.900.10, 1 hit
2.30.29.30, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR029212, ARHGEF5/35_N
IPR035899, DBL_dom_sf
IPR000219, DH-domain
IPR001331, GDS_CDC24_CS
IPR011993, PH-like_dom_sf
IPR001849, PH_domain
IPR036028, SH3-like_dom_sf
IPR001452, SH3_domain

Pfam protein domain database

More...Pfami		View protein in Pfam
PF15441, ARHGEF5_35, 1 hit
PF00621, RhoGEF, 1 hit
PF14604, SH3_9, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00233, PH, 1 hit
SM00325, RhoGEF, 1 hit
SM00326, SH3, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF48065, SSF48065, 1 hit
SSF50044, SSF50044, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00741, DH_1, 1 hit
PS50010, DH_2, 1 hit
PS50003, PH_DOMAIN, 1 hit
PS50002, SH3, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket
Note: A number of additional isoforms are detected in primary breast tumors but not in normal tissues.1 Publication

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q12774-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEAEEAQRGA SPPISAIEEF SIIPEAPMRS SQVSALGLEA QEDEDPSYKW 
        60         70         80         90        100
REEHRLSATQ QSELRDVCDY AIETMPSFPK EGSADVEPNQ ESLVAEACDT 
       110        120        130        140        150
PEHWEAVPQS LAGRQARTLA PPELWACPIQ SEHLDMAPFS SDLGSEEEEV 
       160        170        180        190        200
EFWPGLTSLT LGSGQAEEEE ETSSDNSGQT RYYSPCEEHP AETNQNEGSE 
       210        220        230        240        250
SGTIRQGEEL PPEELQESQG LLHPQEVQVL EEQGQQEAGF RGEGTLREDV 
       260        270        280        290        300
CADGLLGEEQ MIEQVNDEKG EQKQKQEQVQ DVMLGRQGER MGLTGEPEGL 
       310        320        330        340        350
NDGEWEQEDM ERKAQGQGGP EQGEERKREL QVPEENRADS QDEKSQTFLG 
       360        370        380        390        400
KSEEVTGKQE DHGIKEKGVP VSGQEAKEPE SWDGGRLGAV GRARSREEEN 
       410        420        430        440        450
EHHGPSMPAL IAPEDSPHCD LFPGASYLMT QIPGTQTESR AEELSPAALS 
       460        470        480        490        500
PSLEPIRCSH QPISLLGSFL TEESPDKEID QNSQQEESRL RKGTVSSQGT 
       510        520        530        540        550
EVVFASASVT PPRTPDSAPP SPAEAYPITP ASVSARPPVA FPRRETSCAA 
       560        570        580        590        600
RAPETASAPL SMDDPSPCGT SEMCPAALYG FPSTGTSPPR PPANSTGTVQ 
       610        620        630        640        650
HLRSDSFPGS HRTEQTPDLV GMLLSYSHSE LPQRPPKPAI YSSVTPRRDR 
       660        670        680        690        700
RSGRDYSTVS ASPTALSTLK QDSQESISNL ERPSSPPSIQ PWVSPHNPAF 
       710        720        730        740        750
ATESPAYGSS PSFVSMEDVR IHEPLPPPPP QRRDTHPSVV ETDGHARVVV 
       760        770        780        790        800
PTLKQHSHPP PLALGSGLHA PHKGPLPQAS DPAVARQHRP LPSTPDSSHH 
       810        820        830        840        850
AQATPRWRYN KPLPPTPDLP QPHLPPISAP GSSRIYRPLP PLPIIDPPTE 
       860        870        880        890        900
PPPLPPKSRG RSRSTRGGHM NSGGHAKTRP ACQDWTVPLP ASAGRTSWPP 
       910        920        930        940        950
ATARSTESFT STSRSKSEVS PGMAFSNMTN FLCPSSPTTP WTPELQGPTS 
       960        970        980        990       1000
KDEAGVSEHP EAPAREPLRR TTPQQGASGP GRSPVGQARQ PEKPSHLHLE 
      1010       1020       1030       1040       1050
KASSWPHRRD SGRPPGDSSG QAVAPSEGAN KHKGWSRQGL RRPSILPEGS 
      1060       1070       1080       1090       1100
SDSRGPAVEK HPGPSDTVVF REKKPKEVMG GFSRRCSKLI NSSQLLYQEY 
      1110       1120       1130       1140       1150
SDVVLNKEIQ SQQRLESLSE TPGPSSPRQP RKALVSSESY LQRLSMASSG 
      1160       1170       1180       1190       1200
SLWQEIPVVR NSTVLLSMTH EDQKLQEVKF ELIVSEASYL RSLNIAVDHF 
      1210       1220       1230       1240       1250
QLSTSLRATL SNQEHQWLFS RLQDVRDVSA TFLSDLEENF ENNIFSFQVC 
      1260       1270       1280       1290       1300
DVVLNHAPDF RRVYLPYVTN QTYQERTFQS LMNSNSNFRE VLEKLESDPV 
      1310       1320       1330       1340       1350
CQRLSLKSFL ILPFQRITRL KLLLQNILKR TQPGSSEEAE ATKAHHALEQ 
      1360       1370       1380       1390       1400
LIRDCNNNVQ SMRRTEELIY LSQKIEFECK IFPLISQSRW LVKSGELTAL 
      1410       1420       1430       1440       1450
EFSASPGLRR KLNTRPVHLH LFNDCLLLSR PREGSRFLVF DHAPFSSIRG 
      1460       1470       1480       1490       1500
EKCEMKLHGP HKNLFRLFLR QNTQGAQAEF LFRTETQSEK LRWISALAMP 
      1510       1520       1530       1540       1550
REELDLLECY NSPQVQCLRA YKPRENDELA LEKADVVMVT QQSSDGWLEG 
      1560       1570       1580       1590 
VRLSDGERGW FPVQQVEFIS NPEVRAQNLK EAHRVKTAKL QLVEQQA
Length:1,597
Mass (Da):176,799
Last modified:March 2, 2010 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i93EC1B1D7E1198C5
GO
Isoform 2 (identifier: Q12774-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1078: Missing.

Show »
Length:519
Mass (Da):60,056
Checksum:i2EE2C16B3F42C053
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y895H0Y895_HUMAN
Rho guanine nucleotide exchange fac...
ARHGEF5
797Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A2R8YF49A0A2R8YF49_HUMAN
Rho guanine nucleotide exchange fac...
ARHGEF5
1,597Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
C9J591C9J591_HUMAN
Rho guanine nucleotide exchange fac...
ARHGEF5
181Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence BAD18708 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti487E → G in BAD18708 (Ref. 2) Curated1
Sequence conflicti575P → Q in BAD18708 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0351751 – 1078Missing in isoform 2. 2 PublicationsAdd BLAST1078

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U02082 mRNA Translation: AAA18010.1
AK160365 mRNA Translation: BAD18708.1 Different initiation.
AC004534 Genomic DNA Translation: AAC12958.1
CH471146 Genomic DNA Translation: EAW80096.1
BC010046 mRNA Translation: AAH10046.1
BC011839 mRNA Translation: AAH11839.1
BC014555 mRNA Translation: AAH14555.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS34771.1 [Q12774-1]

Protein sequence database of the Protein Information Resource

More...PIRi		I38402

NCBI Reference Sequences

More...RefSeqi		NP_005426.2, NM_005435.3 [Q12774-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000056217.10; ENSP00000056217.5; ENSG00000050327.15
ENST00000471847.2; ENSP00000418227.1; ENSG00000050327.15 [Q12774-2]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		7984

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:7984

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

More...MANE-Selecti		ENST00000056217.10; ENSP00000056217.5; NM_005435.4; NP_005426.2

UCSC genome browser

More...UCSCi		uc003wel.4, human [Q12774-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02082 mRNA Translation: AAA18010.1
AK160365 mRNA Translation: BAD18708.1 Different initiation.
AC004534 Genomic DNA Translation: AAC12958.1
CH471146 Genomic DNA Translation: EAW80096.1
BC010046 mRNA Translation: AAH10046.1
BC011839 mRNA Translation: AAH11839.1
BC014555 mRNA Translation: AAH14555.1
CCDSiCCDS34771.1 [Q12774-1]
PIRiI38402
RefSeqiNP_005426.2, NM_005435.3 [Q12774-1]

3D structure databases

AlphaFoldDBiQ12774
SMRiQ12774
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi113696, 80 interactors
IntActiQ12774, 39 interactors
MINTiQ12774
STRINGi9606.ENSP00000056217

PTM databases

iPTMnetiQ12774
PhosphoSitePlusiQ12774

Genetic variation databases

BioMutaiARHGEF5
DMDMi290457679

Proteomic databases

EPDiQ12774
jPOSTiQ12774
MassIVEiQ12774
MaxQBiQ12774
PaxDbiQ12774
PeptideAtlasiQ12774
PRIDEiQ12774
ProteomicsDBi58920 [Q12774-1]
58921 [Q12774-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		18513, 334 antibodies from 36 providers

The DNASU plasmid repository

More...DNASUi		7984

Genome annotation databases

EnsembliENST00000056217.10; ENSP00000056217.5; ENSG00000050327.15
ENST00000471847.2; ENSP00000418227.1; ENSG00000050327.15 [Q12774-2]
GeneIDi7984
KEGGihsa:7984
MANE-SelectiENST00000056217.10; ENSP00000056217.5; NM_005435.4; NP_005426.2
UCSCiuc003wel.4, human [Q12774-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		7984
DisGeNETi7984

GeneCards: human genes, protein and diseases

More...GeneCardsi		ARHGEF5
HGNCiHGNC:13209, ARHGEF5
HPAiENSG00000050327, Tissue enhanced (skin)
MIMi600888, gene
neXtProtiNX_Q12774
OpenTargetsiENSG00000050327
PharmGKBiPA24975
VEuPathDBiHostDB:ENSG00000050327

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG3523, Eukaryota
GeneTreeiENSGT01030000234571
HOGENOMiCLU_003633_0_0_1
InParanoidiQ12774
OMAiQDDVMLG
OrthoDBi1176939at2759
PhylomeDBiQ12774
TreeFamiTF342609

Enzyme and pathway databases

PathwayCommonsiQ12774
ReactomeiR-HSA-193648, NRAGE signals death through JNK
R-HSA-416482, G alpha (12/13) signalling events
R-HSA-8980692, RHOA GTPase cycle
R-HSA-9013026, RHOB GTPase cycle
R-HSA-9013106, RHOC GTPase cycle
R-HSA-9013148, CDC42 GTPase cycle
R-HSA-9013149, RAC1 GTPase cycle
R-HSA-9013408, RHOG GTPase cycle
SignaLinkiQ12774
SIGNORiQ12774

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		7984, 53 hits in 1063 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		ARHGEF5, human

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		ARHGEF5

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		7984
PharosiQ12774, Tbio

Protein Ontology

More...PROi		PR:Q12774
RNActiQ12774, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000050327, Expressed in thoracic mammary gland and 176 other tissues
ExpressionAtlasiQ12774, baseline and differential
GenevisibleiQ12774, HS

Family and domain databases

CDDicd00160, RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR029212, ARHGEF5/35_N
IPR035899, DBL_dom_sf
IPR000219, DH-domain
IPR001331, GDS_CDC24_CS
IPR011993, PH-like_dom_sf
IPR001849, PH_domain
IPR036028, SH3-like_dom_sf
IPR001452, SH3_domain
PfamiView protein in Pfam
PF15441, ARHGEF5_35, 1 hit
PF00621, RhoGEF, 1 hit
PF14604, SH3_9, 1 hit
SMARTiView protein in SMART
SM00233, PH, 1 hit
SM00325, RhoGEF, 1 hit
SM00326, SH3, 1 hit
SUPFAMiSSF48065, SSF48065, 1 hit
SSF50044, SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS00741, DH_1, 1 hit
PS50010, DH_2, 1 hit
PS50003, PH_DOMAIN, 1 hit
PS50002, SH3, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiARHG5_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q12774
Secondary accession number(s): A6NNJ2, Q6ZML7
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: March 2, 2010
Last modified: May 25, 2022
This is version 189 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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