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UniProtKB - Q12746 (PGA3_YEAST)
Protein
Plasma membrane-associated coenzyme Q6 reductase PGA3
Gene
PGA3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
NADH-dependent cytochrome b5 reductase that reduces coenzyme Q6 at the plasma membrane and mediates lifespan extension by calorie restriction by shifting fermentative to respiratory metabolism, probably through modulating the NAD+/NADH ratio.
2 PublicationsMiscellaneous
Present with 29000 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:1.6.2.21 Publication
Cofactori
FADBy similarity
Activity regulationi
Inhibited by diphenylene iodonium (DPI).1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 153 – 168 | FADBy similarityAdd BLAST | 16 | |
Nucleotide bindingi | 179 – 211 | FADBy similarityAdd BLAST | 33 |
GO - Molecular functioni
GO - Biological processi
- protein transport Source: SGD
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein, NAD |
Names & Taxonomyi
Protein namesi | Recommended name: Plasma membrane-associated coenzyme Q6 reductase PGA3 (EC:1.6.2.2)Alternative name(s): Processing of GAS1 and ALP protein 3 |
Gene namesi | Name:PGA3 Synonyms:NQR1 Ordered Locus Names:YML125C ORF Names:YM4987.10C, YM7056.01C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004594, PGA3 |
VEuPathDBi | FungiDB:YML125C |
Subcellular locationi
Plasma membrane
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 15 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 16 – 36 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 37 – 39 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 40 – 60 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 61 – 179 | ExtracellularSequence analysisAdd BLAST | 119 | |
Transmembranei | 180 – 200 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 201 – 312 | CytoplasmicSequence analysisAdd BLAST | 112 |
Keywords - Cellular componenti
Cell membrane, Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000167628 | 1 – 312 | Plasma membrane-associated coenzyme Q6 reductase PGA3Add BLAST | 312 |
Proteomic databases
MaxQBi | Q12746 |
PaxDbi | Q12746 |
PRIDEi | Q12746 |
PTM databases
iPTMneti | Q12746 |
Expressioni
Inductioni
By calorie restriction.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 35080, 311 interactors |
DIPi | DIP-4519N |
IntActi | Q12746, 2 interactors |
STRINGi | 4932.YML125C |
Miscellaneous databases
RNActi | Q12746, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 70 – 173 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 104 |
Sequence similaritiesi
Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0534, Eukaryota |
GeneTreei | ENSGT00940000176475 |
HOGENOMi | CLU_003827_9_0_1 |
InParanoidi | Q12746 |
OMAi | HAKERCF |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR001834, CBR-like IPR008333, Cbr1-like_FAD-bd_dom IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR19370, PTHR19370, 1 hit |
Pfami | View protein in Pfam PF00970, FAD_binding_6, 1 hit PF00175, NAD_binding_1, 1 hit |
PRINTSi | PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12746-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKEDIEGTN ILDEPVHGIY IPAALFVVGV AITTYMSGEL KILWSLPILF
60 70 80 90 100
MIIFVRAYSA YKRRRSLYPD RWTSLELEDQ TIISKNTALY RFKLKTRLES
110 120 130 140 150
LDIPAGHHVA VRVPIDGKQE VRYYNPISSK LESGYLDLVV KAYVDGKVSK
160 170 180 190 200
YFAGLNSGDT VDFKGPIGTL NYEPNSSKHL GIVAGGSGIT PVLQILNEII
210 220 230 240 250
TVPEDLTKVS LLYANETEND ILLKDELDEM AEKYPHFQVH YVVHYPSDRW
260 270 280 290 300
TGDVGYITKD QMNRYLPEYS EDNRLLICGP DGMNNLALQY AKELGWKVNS
310
TRSSGDDQVF VF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49218 Genomic DNA Translation: CAA89155.1 Z50178 Genomic DNA Translation: CAA90558.1 BK006946 Genomic DNA Translation: DAA09774.1 |
PIRi | S54059 |
RefSeqi | NP_013581.1, NM_001182488.1 |
Genome annotation databases
EnsemblFungii | YML125C_mRNA; YML125C; YML125C |
GeneIDi | 854914 |
KEGGi | sce:YML125C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49218 Genomic DNA Translation: CAA89155.1 Z50178 Genomic DNA Translation: CAA90558.1 BK006946 Genomic DNA Translation: DAA09774.1 |
PIRi | S54059 |
RefSeqi | NP_013581.1, NM_001182488.1 |
3D structure databases
AlphaFoldDBi | Q12746 |
SMRi | Q12746 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35080, 311 interactors |
DIPi | DIP-4519N |
IntActi | Q12746, 2 interactors |
STRINGi | 4932.YML125C |
PTM databases
iPTMneti | Q12746 |
Proteomic databases
MaxQBi | Q12746 |
PaxDbi | Q12746 |
PRIDEi | Q12746 |
Genome annotation databases
EnsemblFungii | YML125C_mRNA; YML125C; YML125C |
GeneIDi | 854914 |
KEGGi | sce:YML125C |
Organism-specific databases
SGDi | S000004594, PGA3 |
VEuPathDBi | FungiDB:YML125C |
Phylogenomic databases
eggNOGi | KOG0534, Eukaryota |
GeneTreei | ENSGT00940000176475 |
HOGENOMi | CLU_003827_9_0_1 |
InParanoidi | Q12746 |
OMAi | HAKERCF |
Miscellaneous databases
PROi | PR:Q12746 |
RNActi | Q12746, protein |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR001834, CBR-like IPR008333, Cbr1-like_FAD-bd_dom IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR19370, PTHR19370, 1 hit |
Pfami | View protein in Pfam PF00970, FAD_binding_6, 1 hit PF00175, NAD_binding_1, 1 hit |
PRINTSi | PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PGA3_YEAST | |
Accessioni | Q12746Primary (citable) accession number: Q12746 Secondary accession number(s): D6W0G0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - SIMILARITY comments
Index of protein domains and families