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Protein

Glutamate synthase [NADH]

Gene

GLT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Forms L-glutamate from L-glutamine and 2-oxoglutarate. Represents an alternative pathway to L-glutamate dehydrogenase for the biosynthesis of L-glutamate. Participates with glutamine synthetase in ammonia assimilation processes. The enzyme is specific for NADH, L-glutamine and 2-oxoglutarate.2 Publications

Miscellaneous

Present with 18900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

2 L-glutamate + NAD+ = L-glutamine + 2-oxoglutarate + NADH.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Inhibited by homocysteine sulfonamide.1 Publication

Kineticsi

  1. KM=280 µM for L-glutamine2 Publications
  2. KM=40 µM for 2-oxoglutarate2 Publications
  3. KM=7 µM for NADH2 Publications

    pH dependencei

    Optimum pH is 7-7.5. Active from pH 6 to 9.2 Publications

    Pathwayi: L-glutamate biosynthesis via GLT pathway

    This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route).
    Proteins known to be involved in this subpathway in this organism are:
    1. Glutamate synthase [NADH] (GLT1)
    This subpathway is part of the pathway L-glutamate biosynthesis via GLT pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route), the pathway L-glutamate biosynthesis via GLT pathway and in Amino-acid biosynthesis.

    Pathwayi: nitrogen metabolism

    This protein is involved in the pathway nitrogen metabolism, which is part of Energy metabolism.
    View all proteins of this organism that are known to be involved in the pathway nitrogen metabolism and in Energy metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei54For GATase activityBy similarity1
    Metal bindingi1185Iron-sulfur (3Fe-4S)By similarity1
    Metal bindingi1191Iron-sulfur (3Fe-4S)By similarity1
    Metal bindingi1196Iron-sulfur (3Fe-4S)By similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi1132 – 1189FMNBy similarityAdd BLAST58
    Nucleotide bindingi1928 – 1942NADSequence analysisAdd BLAST15

    GO - Molecular functioni

    GO - Biological processi

    • ammonia assimilation cycle Source: SGD
    • glutamate biosynthetic process Source: SGD
    • L-glutamate biosynthetic process Source: UniProtKB-UniPathway

    Keywordsi

    Molecular functionOxidoreductase
    Biological processAmino-acid biosynthesis, Glutamate biosynthesis
    Ligand3Fe-4S, FAD, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:YDL171C-MONOMER
    YEAST:YDL171C-MONOMER
    ReactomeiR-SCE-73621 Pyrimidine catabolism
    SABIO-RKiQ12680
    UniPathwayiUPA00045
    UPA00634; UER00690

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate synthase [NADH] (EC:1.4.1.14)
    Alternative name(s):
    NADH-GOGAT
    Gene namesi
    Name:GLT1
    Ordered Locus Names:YDL171C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome IV

    Organism-specific databases

    EuPathDBiFungiDB:YDL171C
    SGDiS000002330 GLT1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    PropeptideiPRO_00000116121 – 531 PublicationAdd BLAST53
    ChainiPRO_000001161354 – 2145Glutamate synthase [NADH]Add BLAST2092

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2070PhosphothreonineCombined sources1

    Keywords - PTMi

    Phosphoprotein, Zymogen

    Proteomic databases

    MaxQBiQ12680
    PaxDbiQ12680
    PRIDEiQ12680

    PTM databases

    iPTMnetiQ12680

    Interactioni

    Subunit structurei

    Homotrimer.1 Publication

    Protein-protein interaction databases

    BioGridi31894, 166 interactors
    DIPiDIP-6490N
    IntActiQ12680, 35 interactors
    MINTiQ12680
    STRINGi4932.YDL171C

    Structurei

    3D structure databases

    ProteinModelPortaliQ12680
    SMRiQ12680
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini54 – 455Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST402

    Coiled coil

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Coiled coili1551 – 1600Sequence analysisAdd BLAST50

    Sequence similaritiesi

    Belongs to the glutamate synthase family.Curated

    Keywords - Domaini

    Coiled coil, Glutamine amidotransferase

    Phylogenomic databases

    GeneTreeiENSGT00500000044896
    HOGENOMiHOG000031559
    InParanoidiQ12680
    KOiK00264
    OMAiRFKTGAM
    OrthoDBiEOG092C00TJ

    Family and domain databases

    CDDicd00982 gltB_C, 1 hit
    cd02808 GltS_FMN, 1 hit
    Gene3Di1.10.1060.10, 1 hit
    2.160.20.60, 1 hit
    3.20.20.70, 5 hits
    3.50.50.60, 2 hits
    3.60.20.10, 2 hits
    InterProiView protein in InterPro
    IPR013785 Aldolase_TIM
    IPR028261 DPD_II
    IPR036188 FAD/NAD-bd_sf
    IPR023753 FAD/NAD-binding_dom
    IPR017932 GATase_2_dom
    IPR002489 Glu_synth_asu_C
    IPR036485 Glu_synth_asu_C_sf
    IPR006982 Glu_synth_centr_N
    IPR012220 Glu_synth_euk
    IPR002932 Glu_synthdom
    IPR006005 Glut_synth_ssu1
    IPR009051 Helical_ferredxn
    IPR029055 Ntn_hydrolases_N
    PfamiView protein in Pfam
    PF14691 Fer4_20, 1 hit
    PF00310 GATase_2, 1 hit
    PF04898 Glu_syn_central, 1 hit
    PF01645 Glu_synthase, 1 hit
    PF01493 GXGXG, 1 hit
    PF07992 Pyr_redox_2, 1 hit
    PIRSFiPIRSF000187 GOGAT, 1 hit
    SUPFAMiSSF46548 SSF46548, 1 hit
    SSF56235 SSF56235, 1 hit
    SSF69336 SSF69336, 1 hit
    TIGRFAMsiTIGR01317 GOGAT_sm_gam, 1 hit
    PROSITEiView protein in PROSITE
    PS51278 GATASE_TYPE_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12680-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPVLKSDNFD PLEEAYEGGT IQNYNDEHHL HKSWANVIPD KRGLYDPDYE
    60 70 80 90 100
    HDACGVGFVA NKHGEQSHKI VTDARYLLVN MTHRGAVSSD GNGDGAGILL
    110 120 130 140 150
    GIPHEFMKRE FKLDLDLDIP EMGKYAVGNV FFKKNEKNNK KNLIKCQKIF
    160 170 180 190 200
    EDLAASFNLS VLGWRNVPVD STILGDVALS REPTILQPLL VPLYDEKQPE
    210 220 230 240 250
    FNETKFRTQL YLLRKEASLQ IGLENWFYVC SLNNTTIVYK GQLTPAQVYN
    260 270 280 290 300
    YYPDLTNAHF KSHMALVHSR FSTNTFPSWD RAQPLRWLAH NGEINTLRGN
    310 320 330 340 350
    KNWMRSREGV MNSATFKDEL DKLYPIIEEG GSDSAALDNV LELLTINGTL
    360 370 380 390 400
    SLPEAVMMMV PEAYHKDMDS DLKAWYDWAA CLMEPWDGPA LLTFTDGRYC
    410 420 430 440 450
    GAILDRNGLR PCRYYITSDD RVICASEVGV IPIENSLVVQ KGKLKPGDLF
    460 470 480 490 500
    LVDTQLGEMV DTKKLKSQIS KRQDFKSWLS KVIKLDDLLS KTANLVPKEF
    510 520 530 540 550
    ISQDSLSLKV QSDPRLLANG YTFEQVTFLL TPMALTGKEA LGSMGNDAPL
    560 570 580 590 600
    ACLNENPVLL YDYFRQLFAQ VTNPPIDPIR EANVMSLECY VGPQGNLLEM
    610 620 630 640 650
    HSSQCDRLLL KSPILHWNEF QALKNIEAAY PSWSVAEIDI TFDKSEGLLG
    660 670 680 690 700
    YTDTIDKITK LASEAIDDGK KILIITDRKM GANRVSISSL IAISCIHHHL
    710 720 730 740 750
    IRNKQRSQVA LILETGEARE IHHFCVLLGY GCDGVYPYLA METLVRMNRE
    760 770 780 790 800
    GLLRNVNNDN DTLEEGQILE NYKHAIDAGI LKVMSKMGIS TLASYKGAQI
    810 820 830 840 850
    FEALGLDNSI VDLCFTGTSS RIRGVTFEYL AQDAFSLHER GYPSRQTISK
    860 870 880 890 900
    SVNLPESGEY HFRDGGYKHV NEPTAIASLQ DTVRNKNDVS WQLYVKKEME
    910 920 930 940 950
    AIRDCTLRGL LELDFENSVS IPLEQVEPWT EIARRFASGA MSYGSISMEA
    960 970 980 990 1000
    HSTLAIAMNR LGAKSNCGEG GEDAERSAVQ ENGDTMRSAI KQVASARFGV
    1010 1020 1030 1040 1050
    TSYYLSDADE IQIKIAQGAK PGEGGELPAH KVSKDIAKTR HSTPNVGLIS
    1060 1070 1080 1090 1100
    PPPHHDIYSI EDLKQLIYDL KCANPRAGIS VKLVSEVGVG IVASGVAKAK
    1110 1120 1130 1140 1150
    ADHILVSGHD GGTGAARWTS VKYAGLPWEL GLAETHQTLV LNDLRRNVVV
    1160 1170 1180 1190 1200
    QTDGQLRTGF DIAVAVLLGA ESFTLATVPL IAMGCVMLRR CHLNSCAVGI
    1210 1220 1230 1240 1250
    ATQDPYLRSK FKGQPEHVIN FFYYLIQDLR QIMAKLGFRT IDEMVGHSEK
    1260 1270 1280 1290 1300
    LKKRDDVNAK AINIDLSPIL TPAHVIRPGV PTKFTKKQDH KLHTRLDNKL
    1310 1320 1330 1340 1350
    IDEAEVTLDR GLPVNIDASI INTDRALGST LSYRVSKKFG EDGLPKDTVV
    1360 1370 1380 1390 1400
    VNIEGSAGQS FGAFLASGIT FILNGDANDY VGKGLSGGII VIKPPKDSKF
    1410 1420 1430 1440 1450
    KSDENVIVGN TCFYGATSGT AFISGSAGER FGVRNSGATI VVERIKGNNA
    1460 1470 1480 1490 1500
    FEYMTGGRAI VLSQMESLNA FSGATGGIAY CLTSDYDDFV GKINKDTVEL
    1510 1520 1530 1540 1550
    ESLCDPVEIA FVKNLIQEHW NYTQSDLAAR ILGNFNHYLK DFVKVIPTDY
    1560 1570 1580 1590 1600
    KKVLLKEKAE AAKAKAKATS EYLKKFRSNQ EVDDEVNTLL IANQKAKEQE
    1610 1620 1630 1640 1650
    KKKSITISNK ATLKEPKVVD LEDAVPDSKQ LEKNSERIEK TRGFMIHKRR
    1660 1670 1680 1690 1700
    HETHRDPRTR VNDWKEFTNP ITKKDAKYQT ARCMDCGTPF CLSDTGCPLS
    1710 1720 1730 1740 1750
    NIIPKFNELL FKNQWKLALD KLLETNNFPE FTGRVCPAPC EGACTLGIIE
    1760 1770 1780 1790 1800
    DPVGIKSVER IIIDNAFKEG WIKPCPPSTR TGFTVGVIGS GPAGLACADM
    1810 1820 1830 1840 1850
    LNRAGHTVTV YERSDRCGGL LMYGIPNMKL DKAIVQRRID LLSAEGIDFV
    1860 1870 1880 1890 1900
    TNTEIGKTIS MDELKNKHNA VVYAIGSTIP RDLPIKGREL KNIDFAMQLL
    1910 1920 1930 1940 1950
    ESNTKALLNK DLEIIREKIQ GKKVIVVGGG DTGNDCLGTS VRHGAASVLN
    1960 1970 1980 1990 2000
    FELLPEPPVE RAKDNPWPQW PRVMRVDYGH AEVKEHYGRD PREYCILSKE
    2010 2020 2030 2040 2050
    FIGNDEGEVT AIRTVRVEWK KSQSGVWQMV EIPNSEEIFE ADIILLSMGF
    2060 2070 2080 2090 2100
    VGPELINGND NEVKKTRRGT IATLDDSSYS IDGGKTFACG DCRRGQSLIV
    2110 2120 2130 2140
    WAIQEGRKCA ASVDKFLMDG TTYLPSNGGI VQRDYKLLKE LASQV
    Length:2,145
    Mass (Da):238,102
    Last modified:September 5, 2006 - v2
    Checksum:i78184877D2167A0D
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti166 – 173NVPVDSTI → TSRRFYY in CAA61505 (PubMed:8923741).Curated8
    Sequence conflicti450 – 452FLV → IPS in CAA61505 (PubMed:8923741).Curated3
    Sequence conflicti1753V → L in CAA61505 (PubMed:8923741).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X89221 Genomic DNA Translation: CAA61505.1
    Z67750 Genomic DNA Translation: CAA91574.1
    Z74219 Genomic DNA Translation: CAA98745.1
    BK006938 Genomic DNA Translation: DAA11690.1
    PIRiS61041
    RefSeqiNP_010110.1, NM_001180231.1

    Genome annotation databases

    EnsemblFungiiYDL171C; YDL171C; YDL171C
    GeneIDi851383
    KEGGisce:YDL171C

    Similar proteinsi

    Entry informationi

    Entry nameiGLT1_YEAST
    AccessioniPrimary (citable) accession number: Q12680
    Secondary accession number(s): D6VRI0, Q12290
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: September 5, 2006
    Last modified: June 20, 2018
    This is version 173 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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