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UniProtKB - Q12680 (GLT1_YEAST)
Protein
Glutamate synthase [NADH]
Gene
GLT1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Forms L-glutamate from L-glutamine and 2-oxoglutarate. Represents an alternative pathway to L-glutamate dehydrogenase for the biosynthesis of L-glutamate. Participates with glutamine synthetase in ammonia assimilation processes. The enzyme is specific for NADH, L-glutamine and 2-oxoglutarate.
2 PublicationsMiscellaneous
Present with 18900 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:1.4.1.143 Publications
Cofactori
Protein has several cofactor binding sites:- [3Fe-4S] clusterNote: Binds 1 [3Fe-4S] cluster.
- FAD
- FMN
Activity regulationi
Inhibited by homocysteine sulfonamide.1 Publication
Kineticsi
- KM=280 µM for L-glutamine2 Publications
- KM=40 µM for 2-oxoglutarate2 Publications
- KM=7 µM for NADH2 Publications
pH dependencei
Optimum pH is 7-7.5. Active from pH 6 to 9.2 Publications
: L-glutamate biosynthesis via GLT pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route). This subpathway is part of the pathway L-glutamate biosynthesis via GLT pathway, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route), the pathway L-glutamate biosynthesis via GLT pathway and in Amino-acid biosynthesis.
Pathwayi: nitrogen metabolism
This protein is involved in the pathway nitrogen metabolism, which is part of Energy metabolism.View all proteins of this organism that are known to be involved in the pathway nitrogen metabolism and in Energy metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 54 | For GATase activityBy similarity | 1 | |
Metal bindingi | 1185 | Iron-sulfur (3Fe-4S)By similarity | 1 | |
Metal bindingi | 1191 | Iron-sulfur (3Fe-4S)By similarity | 1 | |
Metal bindingi | 1196 | Iron-sulfur (3Fe-4S)By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 1132 – 1189 | FMNBy similarityAdd BLAST | 58 | |
Nucleotide bindingi | 1928 – 1942 | NADSequence analysisAdd BLAST | 15 |
GO - Molecular functioni
- 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- flavin adenine dinucleotide binding Source: InterPro
- FMN binding Source: InterPro
- glutamate synthase (NADH) activity Source: SGD
- glutamate synthase activity Source: GO_Central
- iron ion binding Source: InterPro
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- ammonia assimilation cycle Source: SGD
- glutamate biosynthetic process Source: SGD
- L-glutamate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Amino-acid biosynthesis, Glutamate biosynthesis |
Ligand | 3Fe-4S, FAD, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD |
Enzyme and pathway databases
SABIO-RKi | Q12680 |
UniPathwayi | UPA00045 UPA00634;UER00690 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate synthase [NADH] (EC:1.4.1.143 Publications)Alternative name(s): NADH-GOGAT |
Gene namesi | Name:GLT1 Ordered Locus Names:YDL171C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002330, GLT1 |
VEuPathDBi | FungiDB:YDL171C |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000011612 | 1 – 53 | 1 PublicationAdd BLAST | 53 | |
ChainiPRO_0000011613 | 54 – 2145 | Glutamate synthase [NADH]Add BLAST | 2092 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2070 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Phosphoprotein, ZymogenProteomic databases
MaxQBi | Q12680 |
PaxDbi | Q12680 |
PRIDEi | Q12680 |
PTM databases
iPTMneti | Q12680 |
Interactioni
Subunit structurei
Homotrimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 31894, 178 interactors |
DIPi | DIP-6490N |
IntActi | Q12680, 35 interactors |
MINTi | Q12680 |
STRINGi | 4932.YDL171C |
Miscellaneous databases
RNActi | Q12680, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 54 – 455 | Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST | 402 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 1551 – 1600 | Sequence analysisAdd BLAST | 50 |
Sequence similaritiesi
Belongs to the glutamate synthase family.Curated
Keywords - Domaini
Coiled coil, Glutamine amidotransferasePhylogenomic databases
eggNOGi | KOG0399, Eukaryota |
GeneTreei | ENSGT00940000172171 |
HOGENOMi | CLU_000422_8_2_1 |
InParanoidi | Q12680 |
OMAi | TVFRLQH |
Family and domain databases
CDDi | cd00982, gltB_C, 1 hit cd02808, GltS_FMN, 1 hit |
Gene3Di | 1.10.1060.10, 1 hit 2.160.20.60, 1 hit 3.20.20.70, 2 hits 3.50.50.60, 2 hits 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR028261, DPD_II IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR017932, GATase_2_dom IPR002489, Glu_synth_asu_C IPR036485, Glu_synth_asu_C_sf IPR006982, Glu_synth_centr_N IPR012220, Glu_synth_euk IPR002932, Glu_synthdom IPR006005, Glut_synth_ssu1 IPR009051, Helical_ferredxn IPR029055, Ntn_hydrolases_N |
Pfami | View protein in Pfam PF14691, Fer4_20, 1 hit PF00310, GATase_2, 1 hit PF04898, Glu_syn_central, 1 hit PF01645, Glu_synthase, 1 hit PF01493, GXGXG, 1 hit PF07992, Pyr_redox_2, 1 hit |
PIRSFi | PIRSF000187, GOGAT, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit SSF69336, SSF69336, 1 hit |
TIGRFAMsi | TIGR01317, GOGAT_sm_gam, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q12680-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPVLKSDNFD PLEEAYEGGT IQNYNDEHHL HKSWANVIPD KRGLYDPDYE
60 70 80 90 100
HDACGVGFVA NKHGEQSHKI VTDARYLLVN MTHRGAVSSD GNGDGAGILL
110 120 130 140 150
GIPHEFMKRE FKLDLDLDIP EMGKYAVGNV FFKKNEKNNK KNLIKCQKIF
160 170 180 190 200
EDLAASFNLS VLGWRNVPVD STILGDVALS REPTILQPLL VPLYDEKQPE
210 220 230 240 250
FNETKFRTQL YLLRKEASLQ IGLENWFYVC SLNNTTIVYK GQLTPAQVYN
260 270 280 290 300
YYPDLTNAHF KSHMALVHSR FSTNTFPSWD RAQPLRWLAH NGEINTLRGN
310 320 330 340 350
KNWMRSREGV MNSATFKDEL DKLYPIIEEG GSDSAALDNV LELLTINGTL
360 370 380 390 400
SLPEAVMMMV PEAYHKDMDS DLKAWYDWAA CLMEPWDGPA LLTFTDGRYC
410 420 430 440 450
GAILDRNGLR PCRYYITSDD RVICASEVGV IPIENSLVVQ KGKLKPGDLF
460 470 480 490 500
LVDTQLGEMV DTKKLKSQIS KRQDFKSWLS KVIKLDDLLS KTANLVPKEF
510 520 530 540 550
ISQDSLSLKV QSDPRLLANG YTFEQVTFLL TPMALTGKEA LGSMGNDAPL
560 570 580 590 600
ACLNENPVLL YDYFRQLFAQ VTNPPIDPIR EANVMSLECY VGPQGNLLEM
610 620 630 640 650
HSSQCDRLLL KSPILHWNEF QALKNIEAAY PSWSVAEIDI TFDKSEGLLG
660 670 680 690 700
YTDTIDKITK LASEAIDDGK KILIITDRKM GANRVSISSL IAISCIHHHL
710 720 730 740 750
IRNKQRSQVA LILETGEARE IHHFCVLLGY GCDGVYPYLA METLVRMNRE
760 770 780 790 800
GLLRNVNNDN DTLEEGQILE NYKHAIDAGI LKVMSKMGIS TLASYKGAQI
810 820 830 840 850
FEALGLDNSI VDLCFTGTSS RIRGVTFEYL AQDAFSLHER GYPSRQTISK
860 870 880 890 900
SVNLPESGEY HFRDGGYKHV NEPTAIASLQ DTVRNKNDVS WQLYVKKEME
910 920 930 940 950
AIRDCTLRGL LELDFENSVS IPLEQVEPWT EIARRFASGA MSYGSISMEA
960 970 980 990 1000
HSTLAIAMNR LGAKSNCGEG GEDAERSAVQ ENGDTMRSAI KQVASARFGV
1010 1020 1030 1040 1050
TSYYLSDADE IQIKIAQGAK PGEGGELPAH KVSKDIAKTR HSTPNVGLIS
1060 1070 1080 1090 1100
PPPHHDIYSI EDLKQLIYDL KCANPRAGIS VKLVSEVGVG IVASGVAKAK
1110 1120 1130 1140 1150
ADHILVSGHD GGTGAARWTS VKYAGLPWEL GLAETHQTLV LNDLRRNVVV
1160 1170 1180 1190 1200
QTDGQLRTGF DIAVAVLLGA ESFTLATVPL IAMGCVMLRR CHLNSCAVGI
1210 1220 1230 1240 1250
ATQDPYLRSK FKGQPEHVIN FFYYLIQDLR QIMAKLGFRT IDEMVGHSEK
1260 1270 1280 1290 1300
LKKRDDVNAK AINIDLSPIL TPAHVIRPGV PTKFTKKQDH KLHTRLDNKL
1310 1320 1330 1340 1350
IDEAEVTLDR GLPVNIDASI INTDRALGST LSYRVSKKFG EDGLPKDTVV
1360 1370 1380 1390 1400
VNIEGSAGQS FGAFLASGIT FILNGDANDY VGKGLSGGII VIKPPKDSKF
1410 1420 1430 1440 1450
KSDENVIVGN TCFYGATSGT AFISGSAGER FGVRNSGATI VVERIKGNNA
1460 1470 1480 1490 1500
FEYMTGGRAI VLSQMESLNA FSGATGGIAY CLTSDYDDFV GKINKDTVEL
1510 1520 1530 1540 1550
ESLCDPVEIA FVKNLIQEHW NYTQSDLAAR ILGNFNHYLK DFVKVIPTDY
1560 1570 1580 1590 1600
KKVLLKEKAE AAKAKAKATS EYLKKFRSNQ EVDDEVNTLL IANQKAKEQE
1610 1620 1630 1640 1650
KKKSITISNK ATLKEPKVVD LEDAVPDSKQ LEKNSERIEK TRGFMIHKRR
1660 1670 1680 1690 1700
HETHRDPRTR VNDWKEFTNP ITKKDAKYQT ARCMDCGTPF CLSDTGCPLS
1710 1720 1730 1740 1750
NIIPKFNELL FKNQWKLALD KLLETNNFPE FTGRVCPAPC EGACTLGIIE
1760 1770 1780 1790 1800
DPVGIKSVER IIIDNAFKEG WIKPCPPSTR TGFTVGVIGS GPAGLACADM
1810 1820 1830 1840 1850
LNRAGHTVTV YERSDRCGGL LMYGIPNMKL DKAIVQRRID LLSAEGIDFV
1860 1870 1880 1890 1900
TNTEIGKTIS MDELKNKHNA VVYAIGSTIP RDLPIKGREL KNIDFAMQLL
1910 1920 1930 1940 1950
ESNTKALLNK DLEIIREKIQ GKKVIVVGGG DTGNDCLGTS VRHGAASVLN
1960 1970 1980 1990 2000
FELLPEPPVE RAKDNPWPQW PRVMRVDYGH AEVKEHYGRD PREYCILSKE
2010 2020 2030 2040 2050
FIGNDEGEVT AIRTVRVEWK KSQSGVWQMV EIPNSEEIFE ADIILLSMGF
2060 2070 2080 2090 2100
VGPELINGND NEVKKTRRGT IATLDDSSYS IDGGKTFACG DCRRGQSLIV
2110 2120 2130 2140
WAIQEGRKCA ASVDKFLMDG TTYLPSNGGI VQRDYKLLKE LASQV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 166 – 173 | NVPVDSTI → TSRRFYY in CAA61505 (PubMed:8923741).Curated | 8 | |
Sequence conflicti | 450 – 452 | FLV → IPS in CAA61505 (PubMed:8923741).Curated | 3 | |
Sequence conflicti | 1753 | V → L in CAA61505 (PubMed:8923741).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89221 Genomic DNA Translation: CAA61505.1 Z67750 Genomic DNA Translation: CAA91574.1 Z74219 Genomic DNA Translation: CAA98745.1 BK006938 Genomic DNA Translation: DAA11690.1 |
PIRi | S61041 |
RefSeqi | NP_010110.1, NM_001180231.1 |
Genome annotation databases
EnsemblFungii | YDL171C_mRNA; YDL171C; YDL171C |
GeneIDi | 851383 |
KEGGi | sce:YDL171C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89221 Genomic DNA Translation: CAA61505.1 Z67750 Genomic DNA Translation: CAA91574.1 Z74219 Genomic DNA Translation: CAA98745.1 BK006938 Genomic DNA Translation: DAA11690.1 |
PIRi | S61041 |
RefSeqi | NP_010110.1, NM_001180231.1 |
3D structure databases
AlphaFoldDBi | Q12680 |
SMRi | Q12680 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 31894, 178 interactors |
DIPi | DIP-6490N |
IntActi | Q12680, 35 interactors |
MINTi | Q12680 |
STRINGi | 4932.YDL171C |
PTM databases
iPTMneti | Q12680 |
Proteomic databases
MaxQBi | Q12680 |
PaxDbi | Q12680 |
PRIDEi | Q12680 |
Genome annotation databases
EnsemblFungii | YDL171C_mRNA; YDL171C; YDL171C |
GeneIDi | 851383 |
KEGGi | sce:YDL171C |
Organism-specific databases
SGDi | S000002330, GLT1 |
VEuPathDBi | FungiDB:YDL171C |
Phylogenomic databases
eggNOGi | KOG0399, Eukaryota |
GeneTreei | ENSGT00940000172171 |
HOGENOMi | CLU_000422_8_2_1 |
InParanoidi | Q12680 |
OMAi | TVFRLQH |
Enzyme and pathway databases
UniPathwayi | UPA00045 UPA00634;UER00690 |
SABIO-RKi | Q12680 |
Miscellaneous databases
PROi | PR:Q12680 |
RNActi | Q12680, protein |
Family and domain databases
CDDi | cd00982, gltB_C, 1 hit cd02808, GltS_FMN, 1 hit |
Gene3Di | 1.10.1060.10, 1 hit 2.160.20.60, 1 hit 3.20.20.70, 2 hits 3.50.50.60, 2 hits 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR028261, DPD_II IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR017932, GATase_2_dom IPR002489, Glu_synth_asu_C IPR036485, Glu_synth_asu_C_sf IPR006982, Glu_synth_centr_N IPR012220, Glu_synth_euk IPR002932, Glu_synthdom IPR006005, Glut_synth_ssu1 IPR009051, Helical_ferredxn IPR029055, Ntn_hydrolases_N |
Pfami | View protein in Pfam PF14691, Fer4_20, 1 hit PF00310, GATase_2, 1 hit PF04898, Glu_syn_central, 1 hit PF01645, Glu_synthase, 1 hit PF01493, GXGXG, 1 hit PF07992, Pyr_redox_2, 1 hit |
PIRSFi | PIRSF000187, GOGAT, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit SSF69336, SSF69336, 1 hit |
TIGRFAMsi | TIGR01317, GOGAT_sm_gam, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GLT1_YEAST | |
Accessioni | Q12680Primary (citable) accession number: Q12680 Secondary accession number(s): D6VRI0, Q12290 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | September 5, 2006 | |
Last modified: | May 25, 2022 | |
This is version 196 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families