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UniProtKB - Q12680 (GLT1_YEAST)

Entry version 190 (02 Dec 2020)
Sequence version 2 (05 Sep 2006)
Previous versions | rss
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Protein

Glutamate synthase [NADH]

Gene

GLT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Forms L-glutamate from L-glutamine and 2-oxoglutarate. Represents an alternative pathway to L-glutamate dehydrogenase for the biosynthesis of L-glutamate. Participates with glutamine synthetase in ammonia assimilation processes. The enzyme is specific for NADH, L-glutamine and 2-oxoglutarate.2 Publications

Miscellaneous

Present with 18900 molecules/cell in log phase SD medium.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by homocysteine sulfonamide.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=280 µM for L-glutamine2 Publications
  2. KM=40 µM for 2-oxoglutarate2 Publications
  3. KM=7 µM for NADH2 Publications

    pH dependencei

    Optimum pH is 7-7.5. Active from pH 6 to 9.2 Publications

    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-glutamate biosynthesis via GLT pathway

    This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route).
    Proteins known to be involved in this subpathway in this organism are:
    1. Glutamate synthase [NADH] (GLT1)
    This subpathway is part of the pathway L-glutamate biosynthesis via GLT pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route), the pathway L-glutamate biosynthesis via GLT pathway and in Amino-acid biosynthesis.

    Pathwayi: nitrogen metabolism

    This protein is involved in the pathway nitrogen metabolism, which is part of Energy metabolism.
    View all proteins of this organism that are known to be involved in the pathway nitrogen metabolism and in Energy metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei54For GATase activityBy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi1185Iron-sulfur (3Fe-4S)By similarity1
    Metal bindingi1191Iron-sulfur (3Fe-4S)By similarity1
    Metal bindingi1196Iron-sulfur (3Fe-4S)By similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi1132 – 1189FMNBy similarityAdd BLAST58
    Nucleotide bindingi1928 – 1942NADSequence analysisAdd BLAST15

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    Complete GO annotation on QuickGO ...

    GO - Biological processi

    Complete GO annotation on QuickGO ...

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionOxidoreductase
    Biological processAmino-acid biosynthesis, Glutamate biosynthesis
    Ligand3Fe-4S, FAD, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...    BioCyci    		MetaCyc:YDL171C-MONOMER

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...    SABIO-RKi    		Q12680

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...    UniPathwayi    		UPA00045
    UPA00634;UER00690

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Glutamate synthase [NADH] (EC:1.4.1.14)
    Alternative name(s):
    NADH-GOGAT
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:GLT1
    Ordered Locus Names:YDL171C
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome IV

    Organism-specific databases

    Eukaryotic Pathogen and Host Database Resources

    More...    EuPathDBi    		FungiDB:YDL171C

    Saccharomyces Genome Database

    More...    SGDi    		S000002330, GLT1

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes a propeptide, which is a part of a protein that is cleaved during maturation or activation. Once cleaved, a propeptide generally has no independent biological function.<p><a href='/help/propep' target='_top'>More...</a></p>PropeptideiPRO_00000116121 – 531 PublicationAdd BLAST53
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000001161354 – 2145Glutamate synthase [NADH]Add BLAST2092

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2070PhosphothreonineCombined sources1

    Keywords - PTMi

    Phosphoprotein, Zymogen

    Proteomic databases

    MaxQB - The MaxQuant DataBase

    More...    MaxQBi    		Q12680

    PaxDb, a database of protein abundance averages across all three domains of life

    More...    PaxDbi    		Q12680

    PRoteomics IDEntifications database

    More...    PRIDEi    		Q12680

    PTM databases

    iPTMnet integrated resource for PTMs in systems biology context

    More...    iPTMneti    		Q12680

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homotrimer.

    1 Publication

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGRID)

    More...    BioGRIDi    		31894, 168 interactors

    Database of interacting proteins

    More...    DIPi    		DIP-6490N

    Protein interaction database and analysis system

    More...    IntActi    		Q12680, 35 interactors

    Molecular INTeraction database

    More...    MINTi    		Q12680

    STRING: functional protein association networks

    More...    STRINGi    		4932.YDL171C

    Miscellaneous databases

    RNAct, Protein-RNA interaction predictions for model organisms.

    More...    RNActi    		Q12680, protein

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...    SMRi    		Q12680

    Database of comparative protein structure models

    More...    ModBasei    		Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini54 – 455Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST402

    Coiled coil

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili1551 – 1600Sequence analysisAdd BLAST50

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the glutamate synthase family.Curated

    Keywords - Domaini

    Coiled coil, Glutamine amidotransferase

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...    eggNOGi    		KOG0399, Eukaryota

    Ensembl GeneTree

    More...    GeneTreei    		ENSGT00940000172171

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...    HOGENOMi    		CLU_000422_8_2_1

    InParanoid: Eukaryotic Ortholog Groups

    More...    InParanoidi    		Q12680

    Identification of Orthologs from Complete Genome Data

    More...    OMAi    		AFYGATS

    Family and domain databases

    Conserved Domains Database

    More...    CDDi    		cd00982, gltB_C, 1 hit
    cd02808, GltS_FMN, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...    Gene3Di    		1.10.1060.10, 1 hit
    2.160.20.60, 1 hit
    3.20.20.70, 2 hits
    3.50.50.60, 2 hits
    3.60.20.10, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...    InterProi    		View protein in InterPro
    IPR013785, Aldolase_TIM
    IPR028261, DPD_II
    IPR036188, FAD/NAD-bd_sf
    IPR023753, FAD/NAD-binding_dom
    IPR017932, GATase_2_dom
    IPR002489, Glu_synth_asu_C
    IPR036485, Glu_synth_asu_C_sf
    IPR006982, Glu_synth_centr_N
    IPR012220, Glu_synth_euk
    IPR002932, Glu_synthdom
    IPR006005, Glut_synth_ssu1
    IPR009051, Helical_ferredxn
    IPR029055, Ntn_hydrolases_N

    Pfam protein domain database

    More...    Pfami    		View protein in Pfam
    PF14691, Fer4_20, 1 hit
    PF00310, GATase_2, 1 hit
    PF04898, Glu_syn_central, 1 hit
    PF01645, Glu_synthase, 1 hit
    PF01493, GXGXG, 1 hit
    PF07992, Pyr_redox_2, 1 hit

    PIRSF; a whole-protein classification database

    More...    PIRSFi    		PIRSF000187, GOGAT, 1 hit

    Superfamily database of structural and functional annotation

    More...    SUPFAMi    		SSF46548, SSF46548, 1 hit
    SSF56235, SSF56235, 1 hit
    SSF69336, SSF69336, 1 hit

    TIGRFAMs; a protein family database

    More...    TIGRFAMsi    		TIGR01317, GOGAT_sm_gam, 1 hit

    PROSITE; a protein domain and family database

    More...    PROSITEi    		View protein in PROSITE
    PS51278, GATASE_TYPE_2, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12680-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MPVLKSDNFD PLEEAYEGGT IQNYNDEHHL HKSWANVIPD KRGLYDPDYE 
            60         70         80         90        100
    HDACGVGFVA NKHGEQSHKI VTDARYLLVN MTHRGAVSSD GNGDGAGILL 
           110        120        130        140        150
    GIPHEFMKRE FKLDLDLDIP EMGKYAVGNV FFKKNEKNNK KNLIKCQKIF 
           160        170        180        190        200
    EDLAASFNLS VLGWRNVPVD STILGDVALS REPTILQPLL VPLYDEKQPE 
           210        220        230        240        250
    FNETKFRTQL YLLRKEASLQ IGLENWFYVC SLNNTTIVYK GQLTPAQVYN 
           260        270        280        290        300
    YYPDLTNAHF KSHMALVHSR FSTNTFPSWD RAQPLRWLAH NGEINTLRGN 
           310        320        330        340        350
    KNWMRSREGV MNSATFKDEL DKLYPIIEEG GSDSAALDNV LELLTINGTL 
           360        370        380        390        400
    SLPEAVMMMV PEAYHKDMDS DLKAWYDWAA CLMEPWDGPA LLTFTDGRYC 
           410        420        430        440        450
    GAILDRNGLR PCRYYITSDD RVICASEVGV IPIENSLVVQ KGKLKPGDLF 
           460        470        480        490        500
    LVDTQLGEMV DTKKLKSQIS KRQDFKSWLS KVIKLDDLLS KTANLVPKEF 
           510        520        530        540        550
    ISQDSLSLKV QSDPRLLANG YTFEQVTFLL TPMALTGKEA LGSMGNDAPL 
           560        570        580        590        600
    ACLNENPVLL YDYFRQLFAQ VTNPPIDPIR EANVMSLECY VGPQGNLLEM 
           610        620        630        640        650
    HSSQCDRLLL KSPILHWNEF QALKNIEAAY PSWSVAEIDI TFDKSEGLLG 
           660        670        680        690        700
    YTDTIDKITK LASEAIDDGK KILIITDRKM GANRVSISSL IAISCIHHHL 
           710        720        730        740        750
    IRNKQRSQVA LILETGEARE IHHFCVLLGY GCDGVYPYLA METLVRMNRE 
           760        770        780        790        800
    GLLRNVNNDN DTLEEGQILE NYKHAIDAGI LKVMSKMGIS TLASYKGAQI 
           810        820        830        840        850
    FEALGLDNSI VDLCFTGTSS RIRGVTFEYL AQDAFSLHER GYPSRQTISK 
           860        870        880        890        900
    SVNLPESGEY HFRDGGYKHV NEPTAIASLQ DTVRNKNDVS WQLYVKKEME 
           910        920        930        940        950
    AIRDCTLRGL LELDFENSVS IPLEQVEPWT EIARRFASGA MSYGSISMEA 
           960        970        980        990       1000
    HSTLAIAMNR LGAKSNCGEG GEDAERSAVQ ENGDTMRSAI KQVASARFGV 
          1010       1020       1030       1040       1050
    TSYYLSDADE IQIKIAQGAK PGEGGELPAH KVSKDIAKTR HSTPNVGLIS 
          1060       1070       1080       1090       1100
    PPPHHDIYSI EDLKQLIYDL KCANPRAGIS VKLVSEVGVG IVASGVAKAK 
          1110       1120       1130       1140       1150
    ADHILVSGHD GGTGAARWTS VKYAGLPWEL GLAETHQTLV LNDLRRNVVV 
          1160       1170       1180       1190       1200
    QTDGQLRTGF DIAVAVLLGA ESFTLATVPL IAMGCVMLRR CHLNSCAVGI 
          1210       1220       1230       1240       1250
    ATQDPYLRSK FKGQPEHVIN FFYYLIQDLR QIMAKLGFRT IDEMVGHSEK 
          1260       1270       1280       1290       1300
    LKKRDDVNAK AINIDLSPIL TPAHVIRPGV PTKFTKKQDH KLHTRLDNKL 
          1310       1320       1330       1340       1350
    IDEAEVTLDR GLPVNIDASI INTDRALGST LSYRVSKKFG EDGLPKDTVV 
          1360       1370       1380       1390       1400
    VNIEGSAGQS FGAFLASGIT FILNGDANDY VGKGLSGGII VIKPPKDSKF 
          1410       1420       1430       1440       1450
    KSDENVIVGN TCFYGATSGT AFISGSAGER FGVRNSGATI VVERIKGNNA 
          1460       1470       1480       1490       1500
    FEYMTGGRAI VLSQMESLNA FSGATGGIAY CLTSDYDDFV GKINKDTVEL 
          1510       1520       1530       1540       1550
    ESLCDPVEIA FVKNLIQEHW NYTQSDLAAR ILGNFNHYLK DFVKVIPTDY 
          1560       1570       1580       1590       1600
    KKVLLKEKAE AAKAKAKATS EYLKKFRSNQ EVDDEVNTLL IANQKAKEQE 
          1610       1620       1630       1640       1650
    KKKSITISNK ATLKEPKVVD LEDAVPDSKQ LEKNSERIEK TRGFMIHKRR 
          1660       1670       1680       1690       1700
    HETHRDPRTR VNDWKEFTNP ITKKDAKYQT ARCMDCGTPF CLSDTGCPLS 
          1710       1720       1730       1740       1750
    NIIPKFNELL FKNQWKLALD KLLETNNFPE FTGRVCPAPC EGACTLGIIE 
          1760       1770       1780       1790       1800
    DPVGIKSVER IIIDNAFKEG WIKPCPPSTR TGFTVGVIGS GPAGLACADM 
          1810       1820       1830       1840       1850
    LNRAGHTVTV YERSDRCGGL LMYGIPNMKL DKAIVQRRID LLSAEGIDFV 
          1860       1870       1880       1890       1900
    TNTEIGKTIS MDELKNKHNA VVYAIGSTIP RDLPIKGREL KNIDFAMQLL 
          1910       1920       1930       1940       1950
    ESNTKALLNK DLEIIREKIQ GKKVIVVGGG DTGNDCLGTS VRHGAASVLN 
          1960       1970       1980       1990       2000
    FELLPEPPVE RAKDNPWPQW PRVMRVDYGH AEVKEHYGRD PREYCILSKE 
          2010       2020       2030       2040       2050
    FIGNDEGEVT AIRTVRVEWK KSQSGVWQMV EIPNSEEIFE ADIILLSMGF 
          2060       2070       2080       2090       2100
    VGPELINGND NEVKKTRRGT IATLDDSSYS IDGGKTFACG DCRRGQSLIV 
          2110       2120       2130       2140 
    WAIQEGRKCA ASVDKFLMDG TTYLPSNGGI VQRDYKLLKE LASQV
    Length:2,145
    Mass (Da):238,102
    Last modified:September 5, 2006 - v2
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i78184877D2167A0D
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti166 – 173NVPVDSTI → TSRRFYY in CAA61505 (PubMed:8923741).Curated8
    Sequence conflicti450 – 452FLV → IPS in CAA61505 (PubMed:8923741).Curated3
    Sequence conflicti1753V → L in CAA61505 (PubMed:8923741).Curated1

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...    EMBLi

    GenBank nucleotide sequence database

    More...    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...    DDBJi
    Links Updated
    		X89221 Genomic DNA Translation: CAA61505.1
    Z67750 Genomic DNA Translation: CAA91574.1
    Z74219 Genomic DNA Translation: CAA98745.1
    BK006938 Genomic DNA Translation: DAA11690.1

    Protein sequence database of the Protein Information Resource

    More...    PIRi    		S61041

    NCBI Reference Sequences

    More...    RefSeqi    		NP_010110.1, NM_001180231.1

    Genome annotation databases

    Ensembl fungal genome annotation project

    More...    EnsemblFungii    		YDL171C_mRNA; YDL171C; YDL171C

    Database of genes from NCBI RefSeq genomes

    More...    GeneIDi    		851383

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...    KEGGi    		sce:YDL171C

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    		X89221 Genomic DNA Translation: CAA61505.1
    Z67750 Genomic DNA Translation: CAA91574.1
    Z74219 Genomic DNA Translation: CAA98745.1
    BK006938 Genomic DNA Translation: DAA11690.1
    PIRiS61041
    RefSeqiNP_010110.1, NM_001180231.1

    3D structure databases

    SMRiQ12680
    ModBaseiSearch...

    Protein-protein interaction databases

    BioGRIDi31894, 168 interactors
    DIPiDIP-6490N
    IntActiQ12680, 35 interactors
    MINTiQ12680
    STRINGi4932.YDL171C

    PTM databases

    iPTMnetiQ12680

    Proteomic databases

    MaxQBiQ12680
    PaxDbiQ12680
    PRIDEiQ12680

    Genome annotation databases

    EnsemblFungiiYDL171C_mRNA; YDL171C; YDL171C
    GeneIDi851383
    KEGGisce:YDL171C

    Organism-specific databases

    EuPathDBiFungiDB:YDL171C
    SGDiS000002330, GLT1

    Phylogenomic databases

    eggNOGiKOG0399, Eukaryota
    GeneTreeiENSGT00940000172171
    HOGENOMiCLU_000422_8_2_1
    InParanoidiQ12680
    OMAiAFYGATS

    Enzyme and pathway databases

    UniPathwayiUPA00045
    UPA00634;UER00690
    BioCyciMetaCyc:YDL171C-MONOMER
    SABIO-RKiQ12680

    Miscellaneous databases

    Protein Ontology

    More...    PROi    		PR:Q12680
    RNActiQ12680, protein

    Family and domain databases

    CDDicd00982, gltB_C, 1 hit
    cd02808, GltS_FMN, 1 hit
    Gene3Di1.10.1060.10, 1 hit
    2.160.20.60, 1 hit
    3.20.20.70, 2 hits
    3.50.50.60, 2 hits
    3.60.20.10, 1 hit
    InterProiView protein in InterPro
    IPR013785, Aldolase_TIM
    IPR028261, DPD_II
    IPR036188, FAD/NAD-bd_sf
    IPR023753, FAD/NAD-binding_dom
    IPR017932, GATase_2_dom
    IPR002489, Glu_synth_asu_C
    IPR036485, Glu_synth_asu_C_sf
    IPR006982, Glu_synth_centr_N
    IPR012220, Glu_synth_euk
    IPR002932, Glu_synthdom
    IPR006005, Glut_synth_ssu1
    IPR009051, Helical_ferredxn
    IPR029055, Ntn_hydrolases_N
    PfamiView protein in Pfam
    PF14691, Fer4_20, 1 hit
    PF00310, GATase_2, 1 hit
    PF04898, Glu_syn_central, 1 hit
    PF01645, Glu_synthase, 1 hit
    PF01493, GXGXG, 1 hit
    PF07992, Pyr_redox_2, 1 hit
    PIRSFiPIRSF000187, GOGAT, 1 hit
    SUPFAMiSSF46548, SSF46548, 1 hit
    SSF56235, SSF56235, 1 hit
    SSF69336, SSF69336, 1 hit
    TIGRFAMsiTIGR01317, GOGAT_sm_gam, 1 hit
    PROSITEiView protein in PROSITE
    PS51278, GATASE_TYPE_2, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...    ProtoNeti    		Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...    MobiDBi    		Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiGLT1_YEAST
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q12680
    Secondary accession number(s): D6VRI0, Q12290
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: September 5, 2006
    Last modified: December 2, 2020
    This is version 190 of the entry and version 2 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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