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Protein

Acyl-CoA desaturase

Gene

OLE1

Organism
Ajellomyces capsulatus (Darling's disease fungus) (Histoplasma capsulatum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stearyl-CoA desaturase that utilizes O2 and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Has high specificity and catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides.1 Publication

Catalytic activityi

Stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O.1 Publication

Cofactori

Fe2+By similarityNote: Expected to bind 2 Fe2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi101Iron 1By similarity1
Metal bindingi106Iron 1By similarity1
Metal bindingi138Iron 1By similarity1
Metal bindingi141Iron 2By similarity1
Metal bindingi142Iron 1By similarity1
Metal bindingi246Iron 2By similarity1
Metal bindingi275Iron 2By similarity1
Metal bindingi278Iron 1By similarity1
Metal bindingi279Iron 2By similarity1
Metal bindingi384Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi410Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transport
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17616

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-CoA desaturase (EC:1.14.19.11 Publication)
Alternative name(s):
Delta(9)-desaturaseCurated
Short name:
Delta-9 desaturaseCurated
Fatty acid desaturaseCurated
Stearoyl-CoA desaturaseCurated
Gene namesi
Name:OLE11 Publication
OrganismiAjellomyces capsulatus (Darling's disease fungus) (Histoplasma capsulatum)
Taxonomic identifieri5037 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesAjellomycetaceaeHistoplasma

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 54CytoplasmicCuratedAdd BLAST54
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Topological domaini76 – 80LumenalCurated5
Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
Topological domaini102 – 110CytoplasmicCurated9
Transmembranei111 – 131HelicalSequence analysisAdd BLAST21
Topological domaini132 – 199LumenalCuratedAdd BLAST68
Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
Topological domaini221 – 476CytoplasmicCuratedAdd BLAST256

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001854041 – 476Acyl-CoA desaturaseAdd BLAST476

Structurei

3D structure databases

ProteinModelPortaliQ12618
SMRiQ12618
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini349 – 427Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST79

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi101 – 106Histidine box-1Curated6
Motifi138 – 142Histidine box-2Curated5
Motifi275 – 279Histidine box-3Curated5

Domaini

The histidine box domains are involved in binding the catalytic metal ions.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

CDDicd03505 Delta9-FADS-like, 1 hit
Gene3Di3.10.120.10, 1 hit
InterProiView protein in InterPro
IPR009160 Acyl-CoA_deSatase_haem/ster-bd
IPR015876 Acyl-CoA_DS
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR005804 FA_desaturase_dom
IPR001522 FADS-1_CS
PANTHERiPTHR11351 PTHR11351, 1 hit
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF00487 FA_desaturase, 1 hit
PIRSFiPIRSF000345 OLE1, 1 hit
PRINTSiPR00075 FACDDSATRASE
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS00476 FATTY_ACID_DESATUR_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q12618-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALNEAPTAS PVAETAAGGK DVVTDAARRP NSEPKKVHIT DTPITLANWH
60 70 80 90 100
KHISWLNVTL IIAIPIYGLV QAYWVPLHLK TALWAVVYYF MTGLGITAGY
110 120 130 140 150
HRLWAHCSYS ATLPLKIYLA AVGGGAVEGS IRWWARGHRA HHRYTDTDKD
160 170 180 190 200
PYSVRKGLLY SHIGWMVMKQ NPKRIGRTEI TDLNEDPVVV WQHRNYLKVV
210 220 230 240 250
IFMGIVFPML VSGLGWGDWF GGFIYAGILR IFFVQQATFC VNSLAHWLGD
260 270 280 290 300
QPFDDRNSPR DHIVTALVTL GEGYHNFHHE FPSDYRNAIE WHQYDPTKWT
310 320 330 340 350
IWIWKQLGLA YDLKQFRANE IEKGRVQQLQ KKIDQRRAKL DWGIPLEQLP
360 370 380 390 400
VIEWDDYVDQ AKNGRGLIAI AGVVHDVTDF IKDHPGGKAM INSGIGKDAT
410 420 430 440 450
AMFNGGVYNH SNAAHNQLST MRVGVIRGGC EVEIWKRAQK ENKEVESVRD
460 470
EYGNRIVRAG AQVTKIPEPI TTADAA
Length:476
Mass (Da):53,791
Last modified:November 1, 1996 - v1
Checksum:iA91A9CE2A865CADB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85963 Genomic DNA Translation: CAA59939.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85963 Genomic DNA Translation: CAA59939.1

3D structure databases

ProteinModelPortaliQ12618
SMRiQ12618
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17616

Family and domain databases

CDDicd03505 Delta9-FADS-like, 1 hit
Gene3Di3.10.120.10, 1 hit
InterProiView protein in InterPro
IPR009160 Acyl-CoA_deSatase_haem/ster-bd
IPR015876 Acyl-CoA_DS
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR005804 FA_desaturase_dom
IPR001522 FADS-1_CS
PANTHERiPTHR11351 PTHR11351, 1 hit
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF00487 FA_desaturase, 1 hit
PIRSFiPIRSF000345 OLE1, 1 hit
PRINTSiPR00075 FACDDSATRASE
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS00476 FATTY_ACID_DESATUR_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACO1_AJECA
AccessioniPrimary (citable) accession number: Q12618
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: November 1, 1996
Last modified: February 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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