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UniProtKB - Q12618 (ACO1_AJECA)
Protein
Acyl-CoA desaturase
Gene
OLE1
Organism
Ajellomyces capsulatus (Darling's disease fungus) (Histoplasma capsulatum)
Status
Functioni
Stearoyl-CoA desaturase that utilizes O2 and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Has high specificity and catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides.
1 PublicationCatalytic activityi
- 2 Fe(II)-[cytochrome b5] + 2 H+ + O2 + octadecanoyl-CoA = (9Z)-octadecenoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2O1 PublicationEC:1.14.19.11 Publication
Cofactori
Fe2+By similarityNote: Expected to bind 2 Fe2+ ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 101 | Iron 1By similarity | 1 | |
Metal bindingi | 106 | Iron 1By similarity | 1 | |
Metal bindingi | 138 | Iron 1By similarity | 1 | |
Metal bindingi | 141 | Iron 2By similarity | 1 | |
Metal bindingi | 142 | Iron 1By similarity | 1 | |
Metal bindingi | 246 | Iron 2By similarity | 1 | |
Metal bindingi | 275 | Iron 2By similarity | 1 | |
Metal bindingi | 278 | Iron 1By similarity | 1 | |
Metal bindingi | 279 | Iron 2By similarity | 1 | |
Metal bindingi | 384 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 410 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- stearoyl-CoA 9-desaturase activity Source: UniProtKB-EC
GO - Biological processi
- unsaturated fatty acid biosynthetic process Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transport |
Ligand | Heme, Iron, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Acyl-CoA desaturase (EC:1.14.19.11 Publication)Alternative name(s): Delta(9)-desaturaseCurated Short name: Delta-9 desaturaseCurated Fatty acid desaturaseCurated Stearoyl-CoA desaturaseCurated |
Gene namesi | Name:OLE11 Publication |
Organismi | Ajellomyces capsulatus (Darling's disease fungus) (Histoplasma capsulatum) |
Taxonomic identifieri | 5037 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › Ajellomycetaceae › Histoplasma |
Subcellular locationi
Other locations
- Membrane Sequence analysis; Multi-pass membrane protein Sequence analysis
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 54 | CytoplasmicCuratedAdd BLAST | 54 | |
Transmembranei | 55 – 75 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 76 – 80 | LumenalCurated | 5 | |
Transmembranei | 81 – 101 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 102 – 110 | CytoplasmicCurated | 9 | |
Transmembranei | 111 – 131 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 132 – 199 | LumenalCuratedAdd BLAST | 68 | |
Transmembranei | 200 – 220 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 221 – 476 | CytoplasmicCuratedAdd BLAST | 256 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185404 | 1 – 476 | Acyl-CoA desaturaseAdd BLAST | 476 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 349 – 427 | Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST | 79 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 35 | DisorderedSequence analysisAdd BLAST | 35 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 101 – 106 | Histidine box-1Curated | 6 | |
Motifi | 138 – 142 | Histidine box-2Curated | 5 | |
Motifi | 275 – 279 | Histidine box-3Curated | 5 |
Domaini
The histidine box domains are involved in binding the catalytic metal ions.By similarity
Sequence similaritiesi
Belongs to the fatty acid desaturase type 1 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd03505, Delta9-FADS-like, 1 hit |
Gene3Di | 3.10.120.10, 1 hit |
InterProi | View protein in InterPro IPR009160, Acyl-CoA_deSatase_haem/ster-bd IPR015876, Acyl-CoA_DS IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR018506, Cyt_B5_heme-BS IPR005804, FA_desaturase_dom IPR001522, FADS-1_CS |
PANTHERi | PTHR11351, PTHR11351, 1 hit |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF00487, FA_desaturase, 1 hit |
PIRSFi | PIRSF000345, OLE1, 1 hit |
PRINTSi | PR00075, FACDDSATRASE |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF55856, SSF55856, 1 hit |
PROSITEi | View protein in PROSITE PS00191, CYTOCHROME_B5_1, 1 hit PS50255, CYTOCHROME_B5_2, 1 hit PS00476, FATTY_ACID_DESATUR_1, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12618-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALNEAPTAS PVAETAAGGK DVVTDAARRP NSEPKKVHIT DTPITLANWH
60 70 80 90 100
KHISWLNVTL IIAIPIYGLV QAYWVPLHLK TALWAVVYYF MTGLGITAGY
110 120 130 140 150
HRLWAHCSYS ATLPLKIYLA AVGGGAVEGS IRWWARGHRA HHRYTDTDKD
160 170 180 190 200
PYSVRKGLLY SHIGWMVMKQ NPKRIGRTEI TDLNEDPVVV WQHRNYLKVV
210 220 230 240 250
IFMGIVFPML VSGLGWGDWF GGFIYAGILR IFFVQQATFC VNSLAHWLGD
260 270 280 290 300
QPFDDRNSPR DHIVTALVTL GEGYHNFHHE FPSDYRNAIE WHQYDPTKWT
310 320 330 340 350
IWIWKQLGLA YDLKQFRANE IEKGRVQQLQ KKIDQRRAKL DWGIPLEQLP
360 370 380 390 400
VIEWDDYVDQ AKNGRGLIAI AGVVHDVTDF IKDHPGGKAM INSGIGKDAT
410 420 430 440 450
AMFNGGVYNH SNAAHNQLST MRVGVIRGGC EVEIWKRAQK ENKEVESVRD
460 470
EYGNRIVRAG AQVTKIPEPI TTADAA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X85963 Genomic DNA Translation: CAA59939.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X85963 Genomic DNA Translation: CAA59939.1 |
3D structure databases
SMRi | Q12618 |
ModBasei | Search... |
Family and domain databases
CDDi | cd03505, Delta9-FADS-like, 1 hit |
Gene3Di | 3.10.120.10, 1 hit |
InterProi | View protein in InterPro IPR009160, Acyl-CoA_deSatase_haem/ster-bd IPR015876, Acyl-CoA_DS IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR018506, Cyt_B5_heme-BS IPR005804, FA_desaturase_dom IPR001522, FADS-1_CS |
PANTHERi | PTHR11351, PTHR11351, 1 hit |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF00487, FA_desaturase, 1 hit |
PIRSFi | PIRSF000345, OLE1, 1 hit |
PRINTSi | PR00075, FACDDSATRASE |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF55856, SSF55856, 1 hit |
PROSITEi | View protein in PROSITE PS00191, CYTOCHROME_B5_1, 1 hit PS50255, CYTOCHROME_B5_2, 1 hit PS00476, FATTY_ACID_DESATUR_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACO1_AJECA | |
Accessioni | Q12618Primary (citable) accession number: Q12618 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 97 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |