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UniProtKB - Q12532 (RQC2_YEAST)

Entry version 151 (07 Apr 2021)
Sequence version 1 (01 Nov 1996)
Previous versions | rss
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Protein

Ribosome quality control complex subunit 2

Gene

RQC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation (PubMed:23178123). RQC2 is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety (PubMed:25349383). RQC2 is important for the stable association of RKR1/LTN1 to the complex (PubMed:23479637). RQC2 recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of stalled nascent chains independently of mRNA or 40S subunits, leading to non-templated C-terminal Ala and Thr extensions (CAT tails). CAT tails induce a HSF1-dependent heat shock response (PubMed:25554787).5 Publications

Miscellaneous

Present with 7700 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processProtein biosynthesis

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ribosome quality control complex subunit 21 Publication
Alternative name(s):
Translation-associated element 21 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:RQC21 Publication
Synonyms:TAE21 Publication
Ordered Locus Names:YPL009C
ORF Names:YP8132.04C
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XVI

Organism-specific databases

Saccharomyces Genome Database

More...SGDi		S000005930, RQC2

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		FungiDB:YPL009C

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi9D → A: Abolishes CAT tail synthesis and heat shock response, but still binds to 60S ribosomal subunits and supports LTN1-dependent ubiquitination of nascent chains; when associated with A-98 and A-99. 1 Publication1
Mutagenesisi98D → A: Abolishes CAT tail synthesis and heat shock response, but still binds to 60S ribosomal subunits and supports LTN1-dependent ubiquitination of nascent chains; when associated with A-9 and A-99. 1 Publication1
Mutagenesisi99R → A: Abolishes CAT tail synthesis and heat shock response, but still binds to 60S ribosomal subunits and supports LTN1-dependent ubiquitination of nascent chains; when associated with A-9 and A-98. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002446271 – 1038Ribosome quality control complex subunit 2Add BLAST1038

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei797PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q12532

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q12532

PRoteomics IDEntifications database

More...PRIDEi		Q12532

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q12532

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the ribosome quality control complex (RQC), composed of the E3 ubiquitin ligase RKR1/LTN1, RQC1 and RQC2, as well as CDC48 and its ubiquitin-binding cofactors. RQC forms a stable complex with 60S ribosomal subunits (PubMed:23178123, PubMed:23479637, PubMed:25349383). RQC2 binds to the 40S-binding surface of tRNAs (PubMed:25554787).

4 Publications

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		36168, 181 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-3265, Ribosome quality control complex

Database of interacting proteins

More...DIPi		DIP-6570N

Protein interaction database and analysis system

More...IntActi		Q12532, 21 interactors

Molecular INTeraction database

More...MINTi		Q12532

STRING: functional protein association networks

More...STRINGi		4932.YPL009C

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q12532, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q12532

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili350 – 383Sequence analysisAdd BLAST34
Coiled coili713 – 768Sequence analysisAdd BLAST56
Coiled coili830 – 912Sequence analysisAdd BLAST83

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi720 – 752Poly-GluAdd BLAST33

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the NEMF family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG2030, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00390000018516

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_003612_1_1_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q12532

Identification of Orthologs from Complete Genome Data

More...OMAi		WVNADQV

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR021846, NFACT-C
IPR008532, NFACT_RNA-bd

Pfam protein domain database

More...Pfami		View protein in Pfam
PF11923, NFACT-C, 1 hit
PF05670, NFACT-R_1, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q12532-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKQRISALDL LLLARELKQD LEGYRLSNIY NIADSSKQFL LKFNKPDSKL 
        60         70         80         90        100
NVVVDCGLRI YLTEFSRPIP PTPSGFVVKL RKHLKAKRLT ALKQVDQDRI 
       110        120        130        140        150
LVLQFADGHF YLVLEFFSAG NVILLDENRR IMALQRVVLE HENKVGQIYE 
       160        170        180        190        200
MFDESLFTTN NESADESIEK NRKAEYTSEL VNEWIKAVQA KYESDITVIK 
       210        220        230        240        250
QLNIQGKEGA KKKKVKVPSI HKLLLSKVPH LSSDLLSKNL KVFNIDPSES 
       260        270        280        290        300
CLNLLEETDS LAELLNSTQL EYNQLLTTTD RKGYILAKRN ENYISEKDTA 
       310        320        330        340        350
DLEFIYDTFH PFKPYINGGD TDSSCIIEVE GPYNRTLDKF FSTIESSKYA 
       360        370        380        390        400
LRIQNQESQA QKKIDDARAE NDRKIQALLD VQELNERKGH LIIENAPLIE 
       410        420        430        440        450
EVKLAVQGLI DQQMDWNTIE KLIKSEQKKG NRIAQLLNLP LNLKQNKISV 
       460        470        480        490        500
KLDLSSKELN TSSDEDNESE GNTTDSSSDS DSEDMESSKE RSTKSMKRKS 
       510        520        530        540        550
NEKINVTIDL GLSAYANATE YFNIKKTSAQ KQKKVEKNVG KAMKNIEVKI 
       560        570        580        590        600
DQQLKKKLKD SHSVLKKIRT PYFFEKYSWF ISSEGFLVMM GKSPAETDQI 
       610        620        630        640        650
YSKYIEDDDI YMSNSFNSHV WIKNPEKTEV PPNTLMQAGI LCMSSSEAWS 
       660        670        680        690        700
KKISSSPWWC FAKNVSKFDG SDNSILPEGA FRLKNENDQN HLPPAQLVMG 
       710        720        730        740        750
FGFLWKVKTS GNEDNGDDDE EEEEEEEEEE EEEEEEEEEE EEEKEEEEKE 
       760        770        780        790        800
EEQQQDEDDS NEVNGLEKGG DSNDSTKNNS FEHDNLEKDI EKHCTISSDT 
       810        820        830        840        850
DSDSGNAKAK NDNSSTQRIL DEPGVPISLI ENINSNVRGK RGKLKKIQKK 
       860        870        880        890        900
YADQDETERL LRLEALGTLK GIEKQQQRKK EEIMKREVRE DRKNKREKQR 
       910        920        930        940        950
RLQALKFTKK EKARVNYDKH KSELKPSLDK GDVVDDIIPV FAPWPALLKY 
       960        970        980        990       1000
KYKVKIQPGS AKKTKTLTEI LHYFKSRPLD GSSTDNEMDW PQEHEMIKGL 
      1010       1020       1030 
KEQDLVLLLC VDKLKVTIAG QKSTKNGGNS SKKGKKKR
Length:1,038
Mass (Da):119,069
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC0ADE76922F26BE0
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U33335 Genomic DNA Translation: AAB68096.1
Z48483 Genomic DNA Translation: CAA88377.1
Z71255 Genomic DNA Translation: CAA95032.1
BK006949 Genomic DNA Translation: DAA11419.1

Protein sequence database of the Protein Information Resource

More...PIRi		S52522

NCBI Reference Sequences

More...RefSeqi		NP_015316.1, NM_001183823.1

Genome annotation databases

Ensembl fungal genome annotation project

More...EnsemblFungii		YPL009C_mRNA; YPL009C; YPL009C

Database of genes from NCBI RefSeq genomes

More...GeneIDi		856098

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		sce:YPL009C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33335 Genomic DNA Translation: AAB68096.1
Z48483 Genomic DNA Translation: CAA88377.1
Z71255 Genomic DNA Translation: CAA95032.1
BK006949 Genomic DNA Translation: DAA11419.1
PIRiS52522
RefSeqiNP_015316.1, NM_001183823.1

3D structure databases

SMRiQ12532
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi36168, 181 interactors
ComplexPortaliCPX-3265, Ribosome quality control complex
DIPiDIP-6570N
IntActiQ12532, 21 interactors
MINTiQ12532
STRINGi4932.YPL009C

PTM databases

iPTMnetiQ12532

Proteomic databases

MaxQBiQ12532
PaxDbiQ12532
PRIDEiQ12532

Genome annotation databases

EnsemblFungiiYPL009C_mRNA; YPL009C; YPL009C
GeneIDi856098
KEGGisce:YPL009C

Organism-specific databases

SGDiS000005930, RQC2
VEuPathDBiFungiDB:YPL009C

Phylogenomic databases

eggNOGiKOG2030, Eukaryota
GeneTreeiENSGT00390000018516
HOGENOMiCLU_003612_1_1_1
InParanoidiQ12532
OMAiWVNADQV

Miscellaneous databases

Protein Ontology

More...PROi		PR:Q12532
RNActiQ12532, protein

Family and domain databases

InterProiView protein in InterPro
IPR021846, NFACT-C
IPR008532, NFACT_RNA-bd
PfamiView protein in Pfam
PF11923, NFACT-C, 1 hit
PF05670, NFACT-R_1, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRQC2_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q12532
Secondary accession number(s): D6W403
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: April 7, 2021
This is version 151 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families
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