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Protein

Ribosome quality control complex subunit 2

Gene

RQC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation (PubMed:23178123). RQC2 is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety (PubMed:25349383). RQC2 is important for the stable association of RKR1/LTN1 to the complex (PubMed:23479637). RQC2 recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of stalled nascent chains independently of mRNA or 40S subunits, leading to non-templated C-terminal Ala and Thr extensions (CAT tails). CAT tails induce a HSF1-dependent heat shock response (PubMed:25554787).5 Publications

Miscellaneous

Present with 7700 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • ribosomal large subunit binding Source: SGD
  • tRNA binding Source: SGD

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processProtein biosynthesis

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-33928-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ribosome quality control complex subunit 21 Publication
Alternative name(s):
Translation-associated element 21 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:RQC21 Publication
Synonyms:TAE21 Publication
Ordered Locus Names:YPL009C
ORF Names:YP8132.04C
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XVI

Organism-specific databases

Saccharomyces Genome Database

More...
SGDi
S000005930 RQC2

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi9D → A: Abolishes CAT tail synthesis and heat shock response, but still binds to 60S ribosomal subunits and supports LTN1-dependent ubiquitination of nascent chains; when associated with A-98 and A-99. 1 Publication1
Mutagenesisi98D → A: Abolishes CAT tail synthesis and heat shock response, but still binds to 60S ribosomal subunits and supports LTN1-dependent ubiquitination of nascent chains; when associated with A-9 and A-99. 1 Publication1
Mutagenesisi99R → A: Abolishes CAT tail synthesis and heat shock response, but still binds to 60S ribosomal subunits and supports LTN1-dependent ubiquitination of nascent chains; when associated with A-9 and A-98. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002446271 – 1038Ribosome quality control complex subunit 2Add BLAST1038

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei797PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q12532

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q12532

PRoteomics IDEntifications database

More...
PRIDEi
Q12532

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q12532

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the ribosome quality control complex (RQC), composed of the E3 ubiquitin ligase RKR1/LTN1, RQC1 and RQC2, as well as CDC48 and its ubiquitin-binding cofactors. RQC forms a stable complex with 60S ribosomal subunits (PubMed:23178123, PubMed:23479637, PubMed:25349383). RQC2 binds to the 40S-binding surface of tRNAs (PubMed:25554787).4 Publications

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
36168, 176 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-3265 RQC complex

Database of interacting proteins

More...
DIPi
DIP-6570N

Protein interaction database and analysis system

More...
IntActi
Q12532, 21 interactors

Molecular INTeraction database

More...
MINTi
Q12532

STRING: functional protein association networks

More...
STRINGi
4932.YPL009C

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
Q12532

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q12532

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and domains’ section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili350 – 383Sequence analysisAdd BLAST34
Coiled coili713 – 768Sequence analysisAdd BLAST56
Coiled coili830 – 912Sequence analysisAdd BLAST83

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi720 – 752Poly-GluAdd BLAST33

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the NEMF family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000018516

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q12532

Identification of Orthologs from Complete Genome Data

More...
OMAi
FEWPKNV

Database of Orthologous Groups

More...
OrthoDBi
EOG092C0NXU

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR021846 NFACT-C
IPR008532 NFACT_RNA-bd

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF11923 NFACT-C, 1 hit
PF05670 NFACT-R_1, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q12532-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKQRISALDL LLLARELKQD LEGYRLSNIY NIADSSKQFL LKFNKPDSKL
60 70 80 90 100
NVVVDCGLRI YLTEFSRPIP PTPSGFVVKL RKHLKAKRLT ALKQVDQDRI
110 120 130 140 150
LVLQFADGHF YLVLEFFSAG NVILLDENRR IMALQRVVLE HENKVGQIYE
160 170 180 190 200
MFDESLFTTN NESADESIEK NRKAEYTSEL VNEWIKAVQA KYESDITVIK
210 220 230 240 250
QLNIQGKEGA KKKKVKVPSI HKLLLSKVPH LSSDLLSKNL KVFNIDPSES
260 270 280 290 300
CLNLLEETDS LAELLNSTQL EYNQLLTTTD RKGYILAKRN ENYISEKDTA
310 320 330 340 350
DLEFIYDTFH PFKPYINGGD TDSSCIIEVE GPYNRTLDKF FSTIESSKYA
360 370 380 390 400
LRIQNQESQA QKKIDDARAE NDRKIQALLD VQELNERKGH LIIENAPLIE
410 420 430 440 450
EVKLAVQGLI DQQMDWNTIE KLIKSEQKKG NRIAQLLNLP LNLKQNKISV
460 470 480 490 500
KLDLSSKELN TSSDEDNESE GNTTDSSSDS DSEDMESSKE RSTKSMKRKS
510 520 530 540 550
NEKINVTIDL GLSAYANATE YFNIKKTSAQ KQKKVEKNVG KAMKNIEVKI
560 570 580 590 600
DQQLKKKLKD SHSVLKKIRT PYFFEKYSWF ISSEGFLVMM GKSPAETDQI
610 620 630 640 650
YSKYIEDDDI YMSNSFNSHV WIKNPEKTEV PPNTLMQAGI LCMSSSEAWS
660 670 680 690 700
KKISSSPWWC FAKNVSKFDG SDNSILPEGA FRLKNENDQN HLPPAQLVMG
710 720 730 740 750
FGFLWKVKTS GNEDNGDDDE EEEEEEEEEE EEEEEEEEEE EEEKEEEEKE
760 770 780 790 800
EEQQQDEDDS NEVNGLEKGG DSNDSTKNNS FEHDNLEKDI EKHCTISSDT
810 820 830 840 850
DSDSGNAKAK NDNSSTQRIL DEPGVPISLI ENINSNVRGK RGKLKKIQKK
860 870 880 890 900
YADQDETERL LRLEALGTLK GIEKQQQRKK EEIMKREVRE DRKNKREKQR
910 920 930 940 950
RLQALKFTKK EKARVNYDKH KSELKPSLDK GDVVDDIIPV FAPWPALLKY
960 970 980 990 1000
KYKVKIQPGS AKKTKTLTEI LHYFKSRPLD GSSTDNEMDW PQEHEMIKGL
1010 1020 1030
KEQDLVLLLC VDKLKVTIAG QKSTKNGGNS SKKGKKKR
Length:1,038
Mass (Da):119,069
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC0ADE76922F26BE0
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U33335 Genomic DNA Translation: AAB68096.1
Z48483 Genomic DNA Translation: CAA88377.1
Z71255 Genomic DNA Translation: CAA95032.1
BK006949 Genomic DNA Translation: DAA11419.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S52522

NCBI Reference Sequences

More...
RefSeqi
NP_015316.1, NM_001183823.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YPL009C_mRNA; YPL009C_mRNA; YPL009C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
856098

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YPL009C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33335 Genomic DNA Translation: AAB68096.1
Z48483 Genomic DNA Translation: CAA88377.1
Z71255 Genomic DNA Translation: CAA95032.1
BK006949 Genomic DNA Translation: DAA11419.1
PIRiS52522
RefSeqiNP_015316.1, NM_001183823.1

3D structure databases

ProteinModelPortaliQ12532
SMRiQ12532
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36168, 176 interactors
ComplexPortaliCPX-3265 RQC complex
DIPiDIP-6570N
IntActiQ12532, 21 interactors
MINTiQ12532
STRINGi4932.YPL009C

PTM databases

iPTMnetiQ12532

Proteomic databases

MaxQBiQ12532
PaxDbiQ12532
PRIDEiQ12532

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL009C_mRNA; YPL009C_mRNA; YPL009C
GeneIDi856098
KEGGisce:YPL009C

Organism-specific databases

SGDiS000005930 RQC2

Phylogenomic databases

GeneTreeiENSGT00390000018516
InParanoidiQ12532
OMAiFEWPKNV
OrthoDBiEOG092C0NXU

Enzyme and pathway databases

BioCyciYEAST:G3O-33928-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q12532

Family and domain databases

InterProiView protein in InterPro
IPR021846 NFACT-C
IPR008532 NFACT_RNA-bd
PfamiView protein in Pfam
PF11923 NFACT-C, 1 hit
PF05670 NFACT-R_1, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRQC2_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q12532
Secondary accession number(s): D6W403
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: December 5, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
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