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UniProtKB - Q12522 (IF6_YEAST)
Protein
Eukaryotic translation initiation factor 6
Gene
TIF6
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by the GTPase RIA1/EFL1 and by SDO1. Also required for pre-rRNA processing.
UniRule annotation6 PublicationsMiscellaneous
Present with 18600 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- ribosomal large subunit binding Source: SGD
- ribosome binding Source: InterPro
- translation initiation factor activity Source: UniProtKB-UniRule
GO - Biological processi
- assembly of large subunit precursor of preribosome Source: SGD
- maturation of 5.8S rRNA Source: GO_Central
- maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
- maturation of LSU-rRNA Source: GO_Central
- maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
- mature ribosome assembly Source: UniProtKB-UniRule
- ribosomal large subunit biogenesis Source: SGD
- ribosomal subunit export from nucleus Source: SGD
- rRNA processing Source: SGD
Keywordsi
Molecular function | Initiation factor |
Biological process | Protein biosynthesis, Ribosome biogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic translation initiation factor 6UniRule annotationShort name: eIF-6UniRule annotation |
Gene namesi | Ordered Locus Names:YPR016C ORF Names:LPZ15C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006220, TIF6 |
VEuPathDBi | FungiDB:YPR016C |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Nucleus
Other locations
- cytoplasm Source: SGD
- preribosome, large subunit precursor Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000153742 | 1 – 245 | Eukaryotic translation initiation factor 6Add BLAST | 245 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 174 | Phosphoserine; by CK1UniRule annotation | 1 | |
Modified residuei | 175 | Phosphoserine; by CK1UniRule annotation | 1 | |
Modified residuei | 231 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.UniRule annotation2 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q12522 |
PaxDbi | Q12522 |
PRIDEi | Q12522 |
PTM databases
iPTMneti | Q12522 |
Interactioni
Subunit structurei
Monomer. Associates with the 60S ribosomal subunit.
Binary interactionsi
Q12522
With | #Exp. | IntAct |
---|---|---|
LSG1 [P53145] | 5 | EBI-9046,EBI-23885 |
NOG2 [P53742] | 4 | EBI-9046,EBI-28532 |
Protein-protein interaction databases
BioGRIDi | 36193, 615 interactors |
DIPi | DIP-5395N |
IntActi | Q12522, 88 interactors |
MINTi | Q12522 |
STRINGi | 4932.YPR016C |
Miscellaneous databases
RNActi | Q12522, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q12522 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q12522 |
Family & Domainsi
Sequence similaritiesi
Belongs to the eIF-6 family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG3185, Eukaryota |
GeneTreei | ENSGT00390000015972 |
HOGENOMi | CLU_071894_0_0_1 |
InParanoidi | Q12522 |
OMAi | GEDTTGP |
Family and domain databases
CDDi | cd00527, IF6, 1 hit |
HAMAPi | MF_00032, eIF_6, 1 hit |
InterProi | View protein in InterPro IPR002769, eIF6 |
PANTHERi | PTHR10784, PTHR10784, 1 hit |
Pfami | View protein in Pfam PF01912, eIF-6, 1 hit |
PIRSFi | PIRSF006413, IF-6, 1 hit |
SMARTi | View protein in SMART SM00654, eIF6, 1 hit |
TIGRFAMsi | TIGR00323, eIF-6, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12522-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATRTQFENS NEIGVFSKLT NTYCLVAVGG SENFYSAFEA ELGDAIPIVH
60 70 80 90 100
TTIAGTRIIG RMTAGNRRGL LVPTQTTDQE LQHLRNSLPD SVKIQRVEER
110 120 130 140 150
LSALGNVICC NDYVALVHPD IDRETEELIS DVLGVEVFRQ TISGNILVGS
160 170 180 190 200
YCSLSNQGGL VHPQTSVQDQ EELSSLLQVP LVAGTVNRGS SVVGAGMVVN
210 220 230 240
DYLAVTGLDT TAPELSVIES IFRLQDAQPE SISGNLRDTL IETYS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z71255 Genomic DNA Translation: CAA95012.1 Z49919 Genomic DNA Translation: CAA90161.1 U31900 Genomic DNA Translation: AAA97594.1 AY692916 Genomic DNA Translation: AAT92935.1 BK006949 Genomic DNA Translation: DAA11442.1 |
PIRi | S57550 |
RefSeqi | NP_015341.1, NM_001184113.1 |
Genome annotation databases
EnsemblFungii | YPR016C_mRNA; YPR016C; YPR016C |
GeneIDi | 856126 |
KEGGi | sce:YPR016C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z71255 Genomic DNA Translation: CAA95012.1 Z49919 Genomic DNA Translation: CAA90161.1 U31900 Genomic DNA Translation: AAA97594.1 AY692916 Genomic DNA Translation: AAT92935.1 BK006949 Genomic DNA Translation: DAA11442.1 |
PIRi | S57550 |
RefSeqi | NP_015341.1, NM_001184113.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G62 | X-ray | 2.50 | A | 1-224 | [»] | |
2X7N | electron microscopy | 11.80 | B | 1-224 | [»] | |
3J2I | electron microscopy | 11.90 | B | 1-245 | [»] | |
3JCT | electron microscopy | 3.08 | y | 1-245 | [»] | |
4V7F | electron microscopy | 8.70 | m | 1-245 | [»] | |
5FL8 | electron microscopy | 9.50 | m | 1-245 | [»] | |
5H4P | electron microscopy | 3.07 | y | 1-227 | [»] | |
5JCS | electron microscopy | 9.50 | m | 1-245 | [»] | |
5T62 | electron microscopy | 3.30 | X | 1-245 | [»] | |
5Z3G | electron microscopy | 3.65 | N | 1-245 | [»] | |
6C0F | electron microscopy | 3.70 | y | 1-245 | [»] | |
6ELZ | electron microscopy | 3.30 | y | 1-245 | [»] | |
6EM1 | electron microscopy | 3.60 | y | 1-245 | [»] | |
6EM4 | electron microscopy | 4.10 | y | 1-245 | [»] | |
6EM5 | electron microscopy | 4.30 | y | 1-245 | [»] | |
6FT6 | electron microscopy | 3.90 | y | 1-245 | [»] | |
6M62 | electron microscopy | 3.20 | y | 1-245 | [»] | |
6N8J | electron microscopy | 3.50 | y | 1-245 | [»] | |
6N8K | electron microscopy | 3.60 | y | 1-245 | [»] | |
6N8L | electron microscopy | 3.60 | y | 1-245 | [»] | |
6N8M | electron microscopy | 3.50 | X | 1-245 | [»] | |
6N8N | electron microscopy | 3.80 | X | 1-245 | [»] | |
6N8O | electron microscopy | 3.50 | X | 1-245 | [»] | |
6QIK | electron microscopy | 3.10 | n | 1-245 | [»] | |
6QT0 | electron microscopy | 3.40 | n | 1-245 | [»] | |
6QTZ | electron microscopy | 3.50 | n | 1-245 | [»] | |
6R84 | electron microscopy | 3.60 | X | 1-224 | [»] | |
6R86 | electron microscopy | 3.40 | X | 1-224 | [»] | |
6R87 | electron microscopy | 3.40 | X | 1-224 | [»] | |
6RI5 | electron microscopy | 3.30 | n | 1-245 | [»] | |
6RZZ | electron microscopy | 3.20 | n | 1-245 | [»] | |
6S05 | electron microscopy | 3.90 | n | 1-245 | [»] | |
6YLG | electron microscopy | 3.00 | y | 1-245 | [»] | |
6YLH | electron microscopy | 3.10 | y | 1-245 | [»] | |
6YLX | electron microscopy | 3.90 | y | 1-245 | [»] | |
6YLY | electron microscopy | 3.80 | y | 1-245 | [»] | |
7BT6 | electron microscopy | 3.12 | y | 1-245 | [»] | |
7BTB | electron microscopy | 3.22 | y | 1-245 | [»] | |
SMRi | Q12522 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36193, 615 interactors |
DIPi | DIP-5395N |
IntActi | Q12522, 88 interactors |
MINTi | Q12522 |
STRINGi | 4932.YPR016C |
PTM databases
iPTMneti | Q12522 |
Proteomic databases
MaxQBi | Q12522 |
PaxDbi | Q12522 |
PRIDEi | Q12522 |
Genome annotation databases
EnsemblFungii | YPR016C_mRNA; YPR016C; YPR016C |
GeneIDi | 856126 |
KEGGi | sce:YPR016C |
Organism-specific databases
SGDi | S000006220, TIF6 |
VEuPathDBi | FungiDB:YPR016C |
Phylogenomic databases
eggNOGi | KOG3185, Eukaryota |
GeneTreei | ENSGT00390000015972 |
HOGENOMi | CLU_071894_0_0_1 |
InParanoidi | Q12522 |
OMAi | GEDTTGP |
Miscellaneous databases
EvolutionaryTracei | Q12522 |
PROi | PR:Q12522 |
RNActi | Q12522, protein |
Family and domain databases
CDDi | cd00527, IF6, 1 hit |
HAMAPi | MF_00032, eIF_6, 1 hit |
InterProi | View protein in InterPro IPR002769, eIF6 |
PANTHERi | PTHR10784, PTHR10784, 1 hit |
Pfami | View protein in Pfam PF01912, eIF-6, 1 hit |
PIRSFi | PIRSF006413, IF-6, 1 hit |
SMARTi | View protein in SMART SM00654, eIF6, 1 hit |
TIGRFAMsi | TIGR00323, eIF-6, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IF6_YEAST | |
Accessioni | Q12522Primary (citable) accession number: Q12522 Secondary accession number(s): D6W426 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 187 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - Translation initiation factors
List of translation initiation factor entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families