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Protein

Epsin-1

Gene

ENT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to membranes enriched in phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Required for endocytosis and localization of actin. Negatively regulated via phosphorylation.3 Publications

GO - Molecular functioni

  • clathrin binding Source: SGD
  • lipid binding Source: UniProtKB-KW
  • ubiquitin binding Source: SGD

GO - Biological processi

  • actin cortical patch assembly Source: SGD
  • actin filament organization Source: SGD
  • endocytosis Source: SGD

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29555-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-1
Gene namesi
Name:ENT1
Ordered Locus Names:YDL161W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

SGDiS000002320 ENT1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi395T → A or E: No phosphorylation. 1 Publication1
Mutagenesisi415T → A or E: No phosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745241 – 454Epsin-1Add BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei160PhosphothreonineCombined sources1
Modified residuei163PhosphoserineCombined sources1
Modified residuei180PhosphothreonineCombined sources1
Modified residuei328PhosphoserineCombined sources1
Cross-linki357Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei364PhosphothreonineCombined sources1
Modified residuei366PhosphothreonineCombined sources1
Modified residuei384PhosphothreonineCombined sources1
Modified residuei386PhosphothreonineCombined sources1
Modified residuei388PhosphothreonineCombined sources1
Modified residuei395Phosphothreonine; by PRK11 Publication1
Modified residuei415Phosphothreonine; by PRK11 Publication1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ12518
PaxDbiQ12518
PRIDEiQ12518

PTM databases

iPTMnetiQ12518

Interactioni

Subunit structurei

Interacts with EDE1 and PAN1.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31902, 172 interactors
DIPiDIP-957N
ELMiQ12518
IntActiQ12518, 13 interactors
MINTiQ12518
STRINGi4932.YDL161W

Structurei

Secondary structure

1454
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ12518
SMRiQ12518
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 143ENTHPROSITE-ProRule annotationAdd BLAST133
Domaini165 – 184UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini189 – 208UIM 2PROSITE-ProRule annotationAdd BLAST20

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni447 – 454Clathrin-binding8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi211 – 349Gln-richAdd BLAST139

Sequence similaritiesi

Belongs to the epsin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00890000140675
HOGENOMiHOG000216614
InParanoidiQ12518
KOiK12471
OMAiHTGFVQP
OrthoDBiEOG092C4BNK

Family and domain databases

CDDicd03571 ENTH_epsin, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR039416 Epsin_ENTH
IPR003903 UIM_dom
PfamiView protein in Pfam
PF01417 ENTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SM00726 UIM, 2 hits
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
PS50330 UIM, 2 hits

Sequencei

Sequence statusi: Complete.

Q12518-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSKQFVRSAK NLVKGYSSTQ VLVRNATSND NHQVSKDSLI ELAEKSYDSA
60 70 80 90 100
DFFEIMDMLD KRLNDKGKYW RHIAKALTVI DYLIRFGSEN CVLWCRENLY
110 120 130 140 150
IIKTLKEFRH EDDEGIDQGQ IVRVKAKELT ALLSDDERLN EERNMNIKGR
160 170 180 190 200
NRKGRRRRGT GRSDENDDDL QRAISASRLT AEEDERRRKQ DEDYETALQL
210 220 230 240 250
SKEEEELKRL QDLQRMQQQQ GQQQLQQPMY YDIFGNPITP EEYAQFQLQQ
260 270 280 290 300
QQQQQQQQLQ QQPMYYDVFG NPITPEELAQ FQQQQQLQEQ QYLASMQQQQ
310 320 330 340 350
QAMSNNPFAK SEQSSSSPKR NQLVAASSPQ QLQQQKQQEP LIQNRTGNQS
360 370 380 390 400
MTDKYSKLNE LLATGTGIDT FGNVGEARIP AQHTKTGTFI NSQGTGYRQV
410 420 430 440 450
SDDPNHNPFL NSQYTGLPST SVVPTQTGYG FGNQSQQQSQ NNGSNNRGYT

LIDL
Length:454
Mass (Da):52,352
Last modified:November 1, 1996 - v1
Checksum:i37447FB4A6449256
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74210 Genomic DNA Translation: CAA98736.1
Z67750 Genomic DNA Translation: CAA91585.1
BK006938 Genomic DNA Translation: DAA11698.1
PIRiS61052
RefSeqiNP_010120.1, NM_001180221.1

Genome annotation databases

EnsemblFungiiYDL161W; YDL161W; YDL161W
GeneIDi851392
KEGGisce:YDL161W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74210 Genomic DNA Translation: CAA98736.1
Z67750 Genomic DNA Translation: CAA91585.1
BK006938 Genomic DNA Translation: DAA11698.1
PIRiS61052
RefSeqiNP_010120.1, NM_001180221.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AHVelectron microscopy13.60E1-154[»]
5LOZX-ray1.95A17-150[»]
5ONFX-ray2.80A/B/C1-154[»]
ProteinModelPortaliQ12518
SMRiQ12518
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31902, 172 interactors
DIPiDIP-957N
ELMiQ12518
IntActiQ12518, 13 interactors
MINTiQ12518
STRINGi4932.YDL161W

PTM databases

iPTMnetiQ12518

Proteomic databases

MaxQBiQ12518
PaxDbiQ12518
PRIDEiQ12518

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL161W; YDL161W; YDL161W
GeneIDi851392
KEGGisce:YDL161W

Organism-specific databases

SGDiS000002320 ENT1

Phylogenomic databases

GeneTreeiENSGT00890000140675
HOGENOMiHOG000216614
InParanoidiQ12518
KOiK12471
OMAiHTGFVQP
OrthoDBiEOG092C4BNK

Enzyme and pathway databases

BioCyciYEAST:G3O-29555-MONOMER

Miscellaneous databases

PROiPR:Q12518

Family and domain databases

CDDicd03571 ENTH_epsin, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR039416 Epsin_ENTH
IPR003903 UIM_dom
PfamiView protein in Pfam
PF01417 ENTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SM00726 UIM, 2 hits
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
PS50330 UIM, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiENT1_YEAST
AccessioniPrimary (citable) accession number: Q12518
Secondary accession number(s): D6VRI8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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Main funding by: National Institutes of Health

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