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Protein

General negative regulator of transcription subunit 5

Gene

NOT5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

Miscellaneous

Present with 5110 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

Keywordsi

Molecular functionActivator, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-34219-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
General negative regulator of transcription subunit 5
Gene namesi
Name:NOT5
Ordered Locus Names:YPR072W
ORF Names:YP9499.27
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

SGDiS000006276 NOT5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001983381 – 560General negative regulator of transcription subunit 5Add BLAST560

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei306PhosphothreonineCombined sources1
Cross-linki338Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei377PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ12514
PaxDbiQ12514
PRIDEiQ12514

PTM databases

iPTMnetiQ12514

Interactioni

Subunit structurei

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1 and NOT2. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi36245, 73 interactors
ComplexPortaliCPX-1800 Ccr4-Not complex
DIPiDIP-2257N
IntActiQ12514, 24 interactors
MINTiQ12514
STRINGi4932.YPR072W

Structurei

Secondary structure

1560
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ12514
SMRiQ12514
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili3 – 26Sequence analysisAdd BLAST24
Coiled coili37 – 71Sequence analysisAdd BLAST35
Coiled coili124 – 177Sequence analysisAdd BLAST54

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000014743
HOGENOMiHOG000184850
InParanoidiQ12514
KOiK12580
OMAiYHYQGTY
OrthoDBiEOG092C3ROC

Family and domain databases

Gene3Di2.30.30.1020, 1 hit
InterProiView protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR012270 CCR4-NOT_su3/5
IPR007282 NOT2/3/5_C
IPR007207 Not_N
PfamiView protein in Pfam
PF04153 NOT2_3_5, 1 hit
PF04065 Not3, 1 hit
PIRSFiPIRSF005290 NOT_su_3_5, 1 hit

Sequencei

Sequence statusi: Complete.

Q12514-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSQRKLQQDI DKLLKKVKEG IEDFDDIYEK FQSTDPSNSS HREKLESDLK
60 70 80 90 100
REIKKLQKHR DQIKTWLSKE DVKDKQSVLM TNRRLIENGM ERFKSVEKLM
110 120 130 140 150
KTKQFSKEAL TNPDIIKDPK ELKKRDQVLF IHDCLDELQK QLEQYEAQEN
160 170 180 190 200
EEQTERHEFH IANLENILKK LQNNEMDPEP VEEFQDDIKY YVENNDDPDF
210 220 230 240 250
IEYDTIYEDM GCEIQPSSSN NEAPKEGNNQ TSLSSIRSSK KQERSPKKKA
260 270 280 290 300
PQRDVSISDR ATTPIAPGVE SASQSISSTP TPVSTDTPLH TVKDDSIKFD
310 320 330 340 350
NSTLGTPTTH VSMKKKESEN DSEQQLNFPP DRTDEIRKTI QHDVETNAAF
360 370 380 390 400
QNPLFNDELK YWLDSKRYLM QPLQEMSPKM VSQLESSLLN CPDSLDADSP
410 420 430 440 450
CLYTKPLSLP HPTSIFFPNE PIRFVYPYDV PLNLTNNEND TDNKFGKDSK
460 470 480 490 500
AKSKKDDDIY SRTSLARIFM KFDLDTLFFI FYHYQGSYEQ FLAARELFKN
510 520 530 540 550
RNWLFNKVDR CWYYKEIEKL PPGMGKSEEE SWRYFDYKKS WLARRCGNDF
560
VYNEEDFEKL
Length:560
Mass (Da):65,855
Last modified:November 1, 1996 - v1
Checksum:i073137119B3E92BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51033 Genomic DNA Translation: AAB68123.1
Z71255 Genomic DNA Translation: CAA94980.1
Z49219 Genomic DNA Translation: CAA89189.1
AY692887 Genomic DNA Translation: AAT92906.1
BK006949 Genomic DNA Translation: DAA11492.1
PIRiS54093
RefSeqiNP_015397.1, NM_001184169.1

Genome annotation databases

EnsemblFungiiYPR072W; YPR072W; YPR072W
GeneIDi856186
KEGGisce:YPR072W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51033 Genomic DNA Translation: AAB68123.1
Z71255 Genomic DNA Translation: CAA94980.1
Z49219 Genomic DNA Translation: CAA89189.1
AY692887 Genomic DNA Translation: AAT92906.1
BK006949 Genomic DNA Translation: DAA11492.1
PIRiS54093
RefSeqiNP_015397.1, NM_001184169.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BY6X-ray2.80C/F299-560[»]
ProteinModelPortaliQ12514
SMRiQ12514
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36245, 73 interactors
ComplexPortaliCPX-1800 Ccr4-Not complex
DIPiDIP-2257N
IntActiQ12514, 24 interactors
MINTiQ12514
STRINGi4932.YPR072W

PTM databases

iPTMnetiQ12514

Proteomic databases

MaxQBiQ12514
PaxDbiQ12514
PRIDEiQ12514

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR072W; YPR072W; YPR072W
GeneIDi856186
KEGGisce:YPR072W

Organism-specific databases

SGDiS000006276 NOT5

Phylogenomic databases

GeneTreeiENSGT00390000014743
HOGENOMiHOG000184850
InParanoidiQ12514
KOiK12580
OMAiYHYQGTY
OrthoDBiEOG092C3ROC

Enzyme and pathway databases

BioCyciYEAST:G3O-34219-MONOMER

Miscellaneous databases

PROiPR:Q12514

Family and domain databases

Gene3Di2.30.30.1020, 1 hit
InterProiView protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR012270 CCR4-NOT_su3/5
IPR007282 NOT2/3/5_C
IPR007207 Not_N
PfamiView protein in Pfam
PF04153 NOT2_3_5, 1 hit
PF04065 Not3, 1 hit
PIRSFiPIRSF005290 NOT_su_3_5, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNOT5_YEAST
AccessioniPrimary (citable) accession number: Q12514
Secondary accession number(s): D6W476
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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