UniProtKB - Q12476 (AIR2_YEAST)
Protein
Protein AIR2
Gene
AIR2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of the TRAMP (TRF4) complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates like cryptic transcripts generated by RNA polymerase II and III, or hypomethylated pre-tRNAi-Met. Both complexes polyadenylate RNA processing and degradation intermediates of snRNAs, snoRNAs and mRNAs that accumulate in strains lacking a functional exosome. AIR2 also inhibits the methylation of NPL3 mediated by HMT1 through its interaction with HMT1.5 Publications
Miscellaneous
Present with 1800 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 61 – 78 | CCHC-type 1PROSITE-ProRule annotationAdd BLAST | 18 | |
Zinc fingeri | 99 – 116 | CCHC-type 2PROSITE-ProRule annotationAdd BLAST | 18 | |
Zinc fingeri | 162 – 179 | CCHC-type 3PROSITE-ProRule annotationAdd BLAST | 18 |
GO - Molecular functioni
- protein-macromolecule adaptor activity Source: SGD
- RNA binding Source: SGD
- zinc ion binding Source: InterPro
GO - Biological processi
- ncRNA polyadenylation Source: SGD
- nuclear mRNA surveillance of mRNA 3'-end processing Source: SGD
- nuclear polyadenylation-dependent CUT catabolic process Source: SGD
- nuclear polyadenylation-dependent rRNA catabolic process Source: SGD
- nuclear polyadenylation-dependent snoRNA catabolic process Source: SGD
- nuclear polyadenylation-dependent snRNA catabolic process Source: SGD
- nuclear polyadenylation-dependent tRNA catabolic process Source: SGD
- tRNA modification Source: SGD
Keywordsi
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Protein AIR2Alternative name(s): Arginine methyltransferase-interacting RING finger protein 2 |
Gene namesi | Name:AIR2 Ordered Locus Names:YDL175C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YDL175C |
SGDi | S000002334, AIR2 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleolus Source: SGD
- TRAMP complex Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000227604 | 1 – 344 | Protein AIR2Add BLAST | 344 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 31 | PhosphoserineCombined sources | 1 | |
Modified residuei | 49 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q12476 |
PaxDbi | Q12476 |
PRIDEi | Q12476 |
PTM databases
iPTMneti | Q12476 |
Interactioni
Subunit structurei
Component of the TRAMP complex (also called TRF4 complex) composed of at least HUL4, MTR4, PAP2/TRF4 and either AIR1 or AIR2.
Interacts with HMT1 and NPL3. The interaction with NPL3 requires the presence of HMT1.
Interacts directly with PAP2.
5 PublicationsBinary interactionsi
Hide detailsQ12476
With | #Exp. | IntAct |
---|---|---|
MTR4 [P47047] | 7 | EBI-31475,EBI-11592 |
PAP2 [P53632] | 21 | EBI-31475,EBI-19517 |
Protein-protein interaction databases
BioGRIDi | 31891, 151 interactors |
ComplexPortali | CPX-1679, TRAMP complex variant 2 |
DIPi | DIP-2543N |
ELMi | Q12476 |
IntActi | Q12476, 24 interactors |
MINTi | Q12476 |
STRINGi | 4932.YDL175C |
Miscellaneous databases
RNActi | Q12476, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q12476 |
SMRi | Q12476 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q12476 |
Family & Domainsi
Sequence similaritiesi
Belongs to the AIR1 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 61 – 78 | CCHC-type 1PROSITE-ProRule annotationAdd BLAST | 18 | |
Zinc fingeri | 99 – 116 | CCHC-type 2PROSITE-ProRule annotationAdd BLAST | 18 | |
Zinc fingeri | 162 – 179 | CCHC-type 3PROSITE-ProRule annotationAdd BLAST | 18 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG4400, Eukaryota |
HOGENOMi | CLU_049076_1_0_1 |
InParanoidi | Q12476 |
OMAi | NNQDRHQ |
Family and domain databases
InterProi | View protein in InterPro IPR016713, Air1/2_Saccharomycetales IPR001878, Znf_CCHC IPR036875, Znf_CCHC_sf |
Pfami | View protein in Pfam PF00098, zf-CCHC, 3 hits |
PIRSFi | PIRSF018162, PolyA_pol_Air1/2, 1 hit |
SMARTi | View protein in SMART SM00343, ZnF_C2HC, 5 hits |
SUPFAMi | SSF57756, SSF57756, 3 hits |
PROSITEi | View protein in PROSITE PS50158, ZF_CCHC, 3 hits |
i Sequence
Sequence statusi: Complete.
Q12476-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEKNTAPFVV DTAPTTPPDK LVAPSIEEVN SNPNELRALR GQGRYFGVSD
60 70 80 90 100
DDKDAIKEAA PKCNNCSQRG HLKKDCPHII CSYCGATDDH YSRHCPKAIQ
110 120 130 140 150
CSKCDEVGHY RSQCPHKWKK VQCTLCKSKK HSKERCPSIW RAYILVDDNE
160 170 180 190 200
KAKPKVLPFH TIYCYNCGGK GHFGDDCKEK RSSRVPNEDG SAFTGSNLSV
210 220 230 240 250
ELKQEYYRHM NRNSDENEDY QFSESIYDED PLPRPSHKRH SQNDHSHSGR
260 270 280 290 300
NKRRASNFHP PPYQKSNVIQ PTIRGETLSL NNNISKNSRY QNTKVNVSSI
310 320 330 340
SENMYGSRYN PSTYVDNNSI SNSSNYRNYN SYQPYRSGTL GKRR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z74223 Genomic DNA Translation: CAA98749.1 Z67750 Genomic DNA Translation: CAA91570.1 BK006938 Genomic DNA Translation: DAA11687.1 |
PIRi | S61037 |
RefSeqi | NP_010106.1, NM_001180235.1 |
Genome annotation databases
EnsemblFungii | YDL175C_mRNA; YDL175C; YDL175C |
GeneIDi | 851379 |
KEGGi | sce:YDL175C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z74223 Genomic DNA Translation: CAA98749.1 Z67750 Genomic DNA Translation: CAA91570.1 BK006938 Genomic DNA Translation: DAA11687.1 |
PIRi | S61037 |
RefSeqi | NP_010106.1, NM_001180235.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LLI | NMR | - | A | 57-180 | [»] | |
3NYB | X-ray | 2.70 | B | 118-198 | [»] | |
4U4C | X-ray | 2.40 | B | 1-62 | [»] | |
BMRBi | Q12476 | |||||
SMRi | Q12476 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31891, 151 interactors |
ComplexPortali | CPX-1679, TRAMP complex variant 2 |
DIPi | DIP-2543N |
ELMi | Q12476 |
IntActi | Q12476, 24 interactors |
MINTi | Q12476 |
STRINGi | 4932.YDL175C |
PTM databases
iPTMneti | Q12476 |
Proteomic databases
MaxQBi | Q12476 |
PaxDbi | Q12476 |
PRIDEi | Q12476 |
Genome annotation databases
EnsemblFungii | YDL175C_mRNA; YDL175C; YDL175C |
GeneIDi | 851379 |
KEGGi | sce:YDL175C |
Organism-specific databases
EuPathDBi | FungiDB:YDL175C |
SGDi | S000002334, AIR2 |
Phylogenomic databases
eggNOGi | KOG4400, Eukaryota |
HOGENOMi | CLU_049076_1_0_1 |
InParanoidi | Q12476 |
OMAi | NNQDRHQ |
Miscellaneous databases
EvolutionaryTracei | Q12476 |
PROi | PR:Q12476 |
RNActi | Q12476, protein |
Family and domain databases
InterProi | View protein in InterPro IPR016713, Air1/2_Saccharomycetales IPR001878, Znf_CCHC IPR036875, Znf_CCHC_sf |
Pfami | View protein in Pfam PF00098, zf-CCHC, 3 hits |
PIRSFi | PIRSF018162, PolyA_pol_Air1/2, 1 hit |
SMARTi | View protein in SMART SM00343, ZnF_C2HC, 5 hits |
SUPFAMi | SSF57756, SSF57756, 3 hits |
PROSITEi | View protein in PROSITE PS50158, ZF_CCHC, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AIR2_YEAST | |
Accessioni | Q12476Primary (citable) accession number: Q12476 Secondary accession number(s): D6VRH7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 21, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names