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Protein

Tricalbin-1

Gene

TCB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in membrane trafficking.2 Publications

Miscellaneous

Present with 6140 molecules/cell in log phase SD medium.1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1008Calcium 1By similarity1
Metal bindingi1008Calcium 2By similarity1
Metal bindingi1014Calcium 1By similarity1
Metal bindingi1064Calcium 1By similarity1
Metal bindingi1064Calcium 2By similarity1
Metal bindingi1066Calcium 1By similarity1
Metal bindingi1066Calcium 2By similarity1
Metal bindingi1066Calcium 3By similarity1
Metal bindingi1069Calcium 3By similarity1
Metal bindingi1072Calcium 2By similarity1
Metal bindingi1072Calcium 3By similarity1

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: UniProtKB-KW
  • lipid binding Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • endoplasmic reticulum membrane organization Source: SGD
  • endoplasmic reticulum-plasma membrane tethering Source: InterPro
  • lipid transport Source: UniProtKB-KW
  • regulation of phosphatidylinositol dephosphorylation Source: SGD

Keywordsi

Biological processLipid transport, Transport
LigandCalcium, Calcium/phospholipid-binding, Lipid-binding, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33621-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tricalbin-1
Gene namesi
Name:TCB1
Ordered Locus Names:YOR086C
ORF Names:YOR3141c
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR086C
SGDiS000005612 TCB1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 106CytoplasmicSequence analysisAdd BLAST106
Transmembranei107 – 127HelicalSequence analysisAdd BLAST21
Topological domaini128ExtracellularSequence analysis1
Transmembranei129 – 149HelicalSequence analysisAdd BLAST21
Topological domaini150 – 1186CytoplasmicSequence analysisAdd BLAST1037

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002522711 – 1186Tricalbin-1Add BLAST1186

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1000PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12466
PaxDbiQ12466
PRIDEiQ12466

PTM databases

CarbonylDBiQ12466
iPTMnetiQ12466

Interactioni

Subunit structurei

Interacts with TCB2 via its C-terminal domain.1 Publication

Protein-protein interaction databases

BioGridi34484, 117 interactors
DIPiDIP-6292N
IntActiQ12466, 19 interactors
MINTiQ12466
STRINGi4932.YOR086C

Structurei

3D structure databases

ProteinModelPortaliQ12466
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini172 – 375SMP-LTDPROSITE-ProRule annotationAdd BLAST204
Domaini373 – 471C2 1PROSITE-ProRule annotationAdd BLAST99
Domaini645 – 741C2 2PROSITE-ProRule annotationAdd BLAST97
Domaini979 – 1078C2 3PROSITE-ProRule annotationAdd BLAST100

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili795 – 822Sequence analysisAdd BLAST28

Domaini

The C-terminal C2 domain shows Ca2+-dependent phospholipid binding. It binds to phosphatidylserine, phosphatidylinositol and various phosphoinositides. The other C2 domains do not retain all 5 conserved Asp residues found in calcium-binding C2 domains.

Sequence similaritiesi

Belongs to the tricalbin family.Curated

Keywords - Domaini

Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00730000113103
HOGENOMiHOG000248700
InParanoidiQ12466
OMAiRTNNPIW
OrthoDBiEOG092C0K2U

Family and domain databases

CDDicd04044 C2A_Tricalbin-like, 1 hit
cd04052 C2B_Tricalbin-like, 1 hit
cd04045 C2C_Tricalbin-like, 1 hit
cd04040 C2D_Tricalbin-like, 1 hit
Gene3Di2.60.40.150, 4 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR037761 C2A_Tricalbin
IPR037765 C2B_Tricalbin
IPR037762 C2C_Tricalbin
IPR037756 C2D_Tricalbin
IPR031468 SMP_LBD
IPR017147 Tricalbin
PfamiView protein in Pfam
PF00168 C2, 4 hits
PIRSFiPIRSF037232 Tricalbin, 1 hit
PRINTSiPR00360 C2DOMAIN
SMARTiView protein in SMART
SM00239 C2, 4 hits
PROSITEiView protein in PROSITE
PS50004 C2, 3 hits
PS51847 SMP, 1 hit

Sequencei

Sequence statusi: Complete.

Q12466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEDTGVTA PKKPETAQVA NINGIDKLEP PKTKEETESS KSVSSEKAAH
60 70 80 90 100
ASDESFKRSI HEASYVGWKQ IGGWEDKDEL TLDDELMDMT RETFLDNIIP
110 120 130 140 150
DSLYGDWYHS VAIFFIGGVA SFALGHYKFS MGSAFFVIVI TSLLYRTSAK
160 170 180 190 200
KYRGSIRELV QKEFTVQKVE NDYESLEWLN AFLDKYWPIL EPSVSQLIVQ
210 220 230 240 250
QANEQMATNE AIPKFITQLW IDELTLGVKP PRVDLVKTFQ NTASDVVVMD
260 270 280 290 300
WGISFTPHDL CDMSAKQVRN YVNELAVVKA KIFGITIPVS VSDIAFKAHA
310 320 330 340 350
RVKFKLMTPF PHVETVNIQL LKVPDFDFVA TLFGRSIFNW EILAIPGLMT
360 370 380 390 400
LIQKMAKKYM GPILLPPFSL QLNIPQLLSG SNLSIGILEI TVKNAKGLKR
410 420 430 440 450
TSSILNESID PYLSFEFNDI SIAKTRTVRD TLNPVWDETL YVLLNSFTDP
460 470 480 490 500
LTISVYDKRA KLKDKVLGRI QYNLNTLHDK TTQRNLKAQF LRNSKPVGEL
510 520 530 540 550
TFDLRFFPTL EEKKLPDGSV EELPDLNTGI AKVVVEEGSR FAEEEQKVTA
560 570 580 590 600
YVEVYLNAKL VLTTGKATDT GTLKWNSDYE AVIADRRKTR YKFVVKDGKG
610 620 630 640 650
EEIGSTIQTL NDLIDRSQVN KNLIPLKNQK GDIKITTYWR PVRLEIGSNS
660 670 680 690 700
VAYTPPIGAI RVFIEKANDL RNLEKFGTID PYCKVLVNGL SKGRTDFKSQ
710 720 730 740 750
TLNPVWNQVI YVAVTSPNQR ITLQCMDVET VNKDRSLGEF NVNVQDLFKK
760 770 780 790 800
DENDKYEETI DEKAKVGRLV MPKKKPKGTI TYYTSFYPAL PVLTLEEIQD
810 820 830 840 850
LDKVNKKKKA LELRKSAIDE KKISKEDKAK FDQEWNEVKE LEDMYSNRQK
860 870 880 890 900
LDLPELLQYN QGVLAVTVLN GELPDSGLYV QAFFDDNGHP RFVSPRIPSR
910 920 930 940 950
IVKNGWSGDV IIKELDKSIT TFRVAKNKNY NRVEKCVCEV ELPTQELVKN
960 970 980 990 1000
CYYKPSILHL SGEGSAKLML QISWFPIDTK QLPANDLITN SGDLTIMSRS
1010 1020 1030 1040 1050
AENLIASDLN GYSDPYLKYY INNEEDCAYK TKVVKKTLNP KWNDEGTIQI
1060 1070 1080 1090 1100
NNRLNDVLRI KVMDWDSTSA DDTIGTAEIP LNKVKVEGTT ELDVPVEGLE
1110 1120 1130 1140 1150
NAGQDGGMLH LAFSFKPRYT ISVSKREKKV GDIASKGLGT GLKAGTTVIG
1160 1170 1180
GGVGAIGKIK KGVFGGLGSL TNHKKNHEMG EEETKF
Length:1,186
Mass (Da):133,576
Last modified:November 1, 1996 - v1
Checksum:i1CD852549A6D82B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA Translation: CAA64008.1
Z74994 Genomic DNA Translation: CAA99281.1
BK006948 Genomic DNA Translation: DAA10864.1
PIRiS61647
RefSeqiNP_014729.1, NM_001183505.1

Genome annotation databases

EnsemblFungiiYOR086C; YOR086C; YOR086C
GeneIDi854253
KEGGisce:YOR086C

Similar proteinsi

Entry informationi

Entry nameiTCB1_YEAST
AccessioniPrimary (citable) accession number: Q12466
Secondary accession number(s): D6W2E8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: November 1, 1996
Last modified: July 18, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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