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Protein

3-keto-steroid reductase

Gene

ERG27

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the reduction of the keto group on the C-3 of sterols. Also facilitates the association of ERG7 with lipid particles preventing its digestion in the endoplasmic reticulum and the lipid particles.2 Publications

Catalytic activityi

A 3-beta-hydroxysteroid + NADP+ = a 3-oxosteroid + NADPH.

Pathwayi: zymosterol biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (ERG11)
  2. Delta(14)-sterol reductase (ERG24)
  3. Methylsterol monooxygenase (ERG25)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (ERG26)
  5. 3-keto-steroid reductase (ERG27)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei202Proton acceptorBy similarity1

GO - Molecular functioni

  • 3-keto sterol reductase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciMetaCyc:YLR100W-MONOMER
YEAST:YLR100W-MONOMER
ReactomeiR-SCE-191273 Cholesterol biosynthesis
UniPathwayi
UPA00770;UER00758

Names & Taxonomyi

Protein namesi
Recommended name:
3-keto-steroid reductase (EC:1.1.1.270)
Gene namesi
Name:ERG27
Ordered Locus Names:YLR100W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR100W
SGDiS000004090 ERG27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000545951 – 3473-keto-steroid reductaseAdd BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei345PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12452
PaxDbiQ12452
PRIDEiQ12452
TopDownProteomicsiQ12452

PTM databases

iPTMnetiQ12452

Interactioni

Subunit structurei

Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum. Interacts with ERG25 and ERG28. Also interacts with ERG7, but only in lipid particles.3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi31374, 326 interactors
DIPiDIP-1237N
IntActiQ12452, 20 interactors
MINTiQ12452
STRINGi4932.YLR100W

Structurei

3D structure databases

ProteinModelPortaliQ12452
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000013340
HOGENOMiHOG000248600
InParanoidiQ12452
KOiK09827
OMAiHPGIFTS
OrthoDBiEOG092C1OD0

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR038765 Papain_like_cys_pep_sf
IPR002347 SDR_fam
PfamiView protein in Pfam
PF00106 adh_short, 1 hit
SUPFAMiSSF51735 SSF51735, 2 hits
SSF54001 SSF54001, 1 hit

Sequencei

Sequence statusi: Complete.

Q12452-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNRKVAIVTG TNSNLGLNIV FRLIETEDTN VRLTIVVTSR TLPRVQEVIN
60 70 80 90 100
QIKDFYNKSG RVEDLEIDFD YLLVDFTNMV SVLNAYYDIN KKYRAINYLF
110 120 130 140 150
VNAAQGIFDG IDWIGAVKEV FTNPLEAVTN PTYKIQLVGV KSKDDMGLIF
160 170 180 190 200
QANVFGPYYF ISKILPQLTR GKAYIVWISS IMSDPKYLSL NDIELLKTNA
210 220 230 240 250
SYEGSKRLVD LLHLATYKDL KKLGINQYVV QPGIFTSHSF SEYLNFFTYF
260 270 280 290 300
GMLCLFYLAR LLGSPWHNID GYKAANAPVY VTRLANPNFE KQDVKYGSAT
310 320 330 340
SRDGMPYIKT QEIDPTGMSD VFAYIQKKKL EWDEKLKDQI VETRTPI
Length:347
Mass (Da):39,725
Last modified:November 1, 1996 - v1
Checksum:iD9A0589C049D8C49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53876 Genomic DNA Translation: AAB67544.1
Z73272 Genomic DNA Translation: CAA97664.1
BK006945 Genomic DNA Translation: DAA09417.1
PIRiS64936
RefSeqiNP_013201.1, NM_001181987.1

Genome annotation databases

EnsemblFungiiYLR100W; YLR100W; YLR100W
GeneIDi850790
KEGGisce:YLR100W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53876 Genomic DNA Translation: AAB67544.1
Z73272 Genomic DNA Translation: CAA97664.1
BK006945 Genomic DNA Translation: DAA09417.1
PIRiS64936
RefSeqiNP_013201.1, NM_001181987.1

3D structure databases

ProteinModelPortaliQ12452
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31374, 326 interactors
DIPiDIP-1237N
IntActiQ12452, 20 interactors
MINTiQ12452
STRINGi4932.YLR100W

PTM databases

iPTMnetiQ12452

Proteomic databases

MaxQBiQ12452
PaxDbiQ12452
PRIDEiQ12452
TopDownProteomicsiQ12452

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR100W; YLR100W; YLR100W
GeneIDi850790
KEGGisce:YLR100W

Organism-specific databases

EuPathDBiFungiDB:YLR100W
SGDiS000004090 ERG27

Phylogenomic databases

GeneTreeiENSGT00390000013340
HOGENOMiHOG000248600
InParanoidiQ12452
KOiK09827
OMAiHPGIFTS
OrthoDBiEOG092C1OD0

Enzyme and pathway databases

UniPathwayi
UPA00770;UER00758

BioCyciMetaCyc:YLR100W-MONOMER
YEAST:YLR100W-MONOMER
ReactomeiR-SCE-191273 Cholesterol biosynthesis

Miscellaneous databases

PROiPR:Q12452

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR038765 Papain_like_cys_pep_sf
IPR002347 SDR_fam
PfamiView protein in Pfam
PF00106 adh_short, 1 hit
SUPFAMiSSF51735 SSF51735, 2 hits
SSF54001 SSF54001, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiERG27_YEAST
AccessioniPrimary (citable) accession number: Q12452
Secondary accession number(s): D6VYA1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: October 10, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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Main funding by: National Institutes of Health

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