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Protein

Anaphase-promoting complex subunit 2

Gene

APC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication.1 Publication

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: SGD
  • cell division Source: UniProtKB-KW
  • exit from mitosis Source: SGD
  • metaphase/anaphase transition of mitotic cell cycle Source: SGD
  • protein ubiquitination Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-32269-MONOMER
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit 2
Gene namesi
Name:APC2
Synonyms:RSI1
Ordered Locus Names:YLR127C
ORF Names:L3105, L3108
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR127C
SGDiS000004117 APC2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198141 – 853Anaphase-promoting complex subunit 2Add BLAST853

Proteomic databases

MaxQBiQ12440
PaxDbiQ12440
PRIDEiQ12440

PTM databases

iPTMnetiQ12440

Interactioni

Subunit structurei

The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1. APC2 interacts directly with APC11 thereby anchoring APC11 to the core complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MND2P405773EBI-33503,EBI-25433

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31396, 137 interactors
ComplexPortaliCPX-756 Anaphase-Promoting Complex variant 1
CPX-760 Anaphase-Promoting Complex variant 2
CPX-761 Anaphase-Promoting Complex variant 3
CPX-762 Anaphase-Promoting Complex variant 4
DIPiDIP-939N
IntActiQ12440, 16 interactors
MINTiQ12440
STRINGi4932.YLR127C

Structurei

Secondary structure

1853
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ12440
SMRiQ12440
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12440

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000016127
HOGENOMiHOG000001122
InParanoidiQ12440
KOiK03349
OMAiSHLYWSS
OrthoDBiEOG092C0FSJ

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR014786 APC_su2_C
IPR016158 Cullin_homology
IPR036317 Cullin_homology_sf
IPR001373 Cullin_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF08672 ANAPC2, 1 hit
PF00888 Cullin, 1 hit
SMARTiView protein in SMART
SM01013 APC2, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF75632 SSF75632, 1 hit
PROSITEiView protein in PROSITE
PS50069 CULLIN_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

Q12440-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSFQITPTRD LKVITDELQT LSSYIFHTNI VDDLNSLLTW MSPNDAKSNH
60 70 80 90 100
QLRPPSLRIK NIIKVLFPNN ATTSPYSMIN TSQANNSIVN EGNTNKELQL
110 120 130 140 150
QLFSTLKEFY IFQVRYHFFL HFNNINYLKD IQRWENYYEF PLRYVPIFDV
160 170 180 190 200
NVNDWALELN SLRHYLLNRN IKFKNNLRTR LDKLIMDDDF DLADNLIQWL
210 220 230 240 250
KSANGSLSST ELIVNALYSK INKFCEDNMS RVWNKRFMIM ETFNKFINQY
260 270 280 290 300
WSQFSKLVGC PEDDHELTTT VFNCFESNFL RIRTNEIFDI CVLAYPDSKV
310 320 330 340 350
TLLELRKIMK DFKDYTNIVT TFLSDFKKYI LNPSVTTVDA LLRYVKTIKA
360 370 380 390 400
FLVLDPTGRC LHSITTFVKP YFQERKHLVN VLLYAMLDLP EEELKEKINF
410 420 430 440 450
NVDMKALLSL VDTLHDSDIN QDTNITKRDK NKKSPFLWNL KVKGKRELNK
460 470 480 490 500
DLPIRHAMLY EHILNYYIAW VPEPNDMIPG NIKSSYIKTN LFEVLLDLFE
510 520 530 540 550
SREFFISEFR NLLTDRLFTL KFYTLDEKWT RCLKLIREKI VKFTETSHSN
560 570 580 590 600
YITNGILGLL ETTAPAADAD QSNLNSIDVM LWDIKCSEEL CRKMHEVAGL
610 620 630 640 650
DPIIFPKFIS LLYWKYNCDT QGSNDLAFHL PIDLERELQK YSDIYSQLKP
660 670 680 690 700
GRKLQLCKDK GKVEIQLAFK DGRKLVLDVS LEQCSVINQF DSPNDEPICL
710 720 730 740 750
SLEQLSESLN IAPPRLTHLL DFWIQKGVLL KENGTYSVIE HSEMDFDQAQ
760 770 780 790 800
KTAPMEIENS NYELHNDSEI ERKYELTLQR SLPFIEGMLT NLGAMKLHKI
810 820 830 840 850
HSFLKITVPK DWGYNRITLQ QLEGYLNTLA DEGRLKYIAN GSYEIVKNGH

KNS
Length:853
Mass (Da):99,978
Last modified:November 1, 1996 - v1
Checksum:iE559A0E28C784DE2
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q12288YL126_YEAST
Putative glutamine amidotransferase...
YLR126C, L3105
251Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89514 Genomic DNA Translation: CAA61705.1
Z73299 Genomic DNA Translation: CAA97696.1
U53877 Genomic DNA Translation: AAB82373.1
X91258 Genomic DNA Translation: CAA62638.1
Z73300 Genomic DNA Translation: CAA97698.1
AY899244 mRNA Translation: AAX83929.1
BK006945 Genomic DNA Translation: DAA09438.1
PIRiS59315
RefSeqiNP_013228.1, NM_001182014.1

Genome annotation databases

EnsemblFungiiYLR127C; YLR127C; YLR127C
GeneIDi850818
KEGGisce:YLR127C

Similar proteinsi

Entry informationi

Entry nameiAPC2_YEAST
AccessioniPrimary (citable) accession number: Q12440
Secondary accession number(s): D6VYC2, Q2VQX4, Q7LGV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 1, 1996
Last modified: September 12, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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