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Entry version 150 (10 Feb 2021)
Sequence version 1 (01 Nov 1996)
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Protein

Diphthine--ammonia ligase

Gene

DPH6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor eEF-2 (EFT1 or EFT2) to diphthamide.2 Publications

Miscellaneous

Present with 2270 molecules/cell in log phase SD medium.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MetaCyc:MONOMER-17855

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
6.3.1.14, 984

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SCE-5358493, Synthesis of diphthamide-EEF2

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00559

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Diphthine--ammonia ligase (EC:6.3.1.14)
Alternative name(s):
Diphthamide synthase
Diphthamide synthetase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:DPH6
Ordered Locus Names:YLR143W
ORF Names:L3177
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XII

Organism-specific databases

Saccharomyces Genome Database

More...
SGDi
S000004133, DPH6

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
FungiDB:YLR143W

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi216G → N: Completely inactivates the enzyme. 1 Publication1
Mutagenesisi220E → A: Completely inactivates the enzyme. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002628691 – 685Diphthine--ammonia ligaseAdd BLAST685

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q12429

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q12429

PRoteomics IDEntifications database

More...
PRIDEi
Q12429

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q12429

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with eEF-2 (EFT1 or EFT2).

1 Publication

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
31412, 74 interactors

Database of interacting proteins

More...
DIPi
DIP-2618N

Protein interaction database and analysis system

More...
IntActi
Q12429, 1 interactor

Molecular INTeraction database

More...
MINTi
Q12429

STRING: functional protein association networks

More...
STRINGi
4932.YLR143W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
Q12429, protein

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the C-terminal section; belongs to the RutC family.Curated
In the N-terminal section; belongs to the Diphthine--ammonia ligase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG2316, Eukaryota
KOG2317, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00420000029820

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_010289_2_1_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q12429

Identification of Orthologs from Complete Genome Data

More...
OMAi
SNSFGRH

Family and domain databases

Conserved Domains Database

More...
CDDi
cd01994, Alpha_ANH_like_IV, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.1330.40, 2 hits
3.40.50.620, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002761, Diphthami_syn_dom
IPR030662, DPH6/MJ0570
IPR014729, Rossmann-like_a/b/a_fold
IPR035959, RutC-like_sf
IPR006175, YjgF/YER057c/UK114

The PANTHER Classification System

More...
PANTHERi
PTHR12196, PTHR12196, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01902, Diphthami_syn_2, 1 hit
PF01042, Ribonuc_L-PSP, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF55298, SSF55298, 2 hits

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00290, MJ0570_dom, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q12429-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKFIALISGG KDSFYNIFHC LKNNHELIAL GNIYPKESEE QELDSFMFQT
60 70 80 90 100
VGHDLIDYYS KCIGVPLFRR SILRNTSNNV ELNYTATQDD EIEELFELLR
110 120 130 140 150
TVKDKIPDLE AVSVGAILSS YQRTRVENVC SRLGLVVLSY LWQRDQAELM
160 170 180 190 200
GEMCLMSKDV NNVENDTNSG NKFDARIIKV AAIGLNEKHL GMSLPMMQPV
210 220 230 240 250
LQKLNQLYQV HICGEGGEFE TMVLDAPFFQ HGYLELIDIV KCSDGEVHNA
260 270 280 290 300
RLKVKFQPRN LSKSFLLNQL DQLPVPSIFG NNWQDLTQNL PKQQAKTGEQ
310 320 330 340 350
RFENHMSNAL PQTTINKTND KLYISNLQSR KSETVEKQSE DIFTELADIL
360 370 380 390 400
HSNQIPRNHI LSASLLIRDM SNFGKINKIY NEFLDLSKYG PLPPSRACVG
410 420 430 440 450
SKCLPEDCHV QLSVVVDVKN TGKEKINKNK GGLHVQGRSY WAPCNIGPYS
460 470 480 490 500
QSTWLNDDAN QVSFISGQIG LVPQSMEILG TPLTDQIVLA LQHFDTLCET
510 520 530 540 550
IGAQEKLLMT CYISDESVLD SVIKTWAFYC SNMNHRSDLW MDKSDDVEKC
560 570 580 590 600
LVLVKISELP RGAVAEFGGV TCKRLIVDDN DSDKKEREEN DDVSTVFQKL
610 620 630 640 650
NLNIEGFHNT TVSAFGYNRN FITGFVDSRE ELELILEKTP KSAQITLYYN
660 670 680
PKEIITFHHH IGYYPVEKLF DYRGKEHRFG LHIRS
Length:685
Mass (Da):77,941
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE3AA0C948A57ADC2
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence CAA62663 differs from that shown. Reason: Frameshift.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U53881 Genomic DNA Translation: AAB82389.1
X91258 Genomic DNA Translation: CAA62663.1 Frameshift.
Z73315 Genomic DNA Translation: CAA97715.1
BK006945 Genomic DNA Translation: DAA09453.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S64985

NCBI Reference Sequences

More...
RefSeqi
NP_013244.1, NM_001182030.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YLR143W_mRNA; YLR143W; YLR143W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
850835

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YLR143W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53881 Genomic DNA Translation: AAB82389.1
X91258 Genomic DNA Translation: CAA62663.1 Frameshift.
Z73315 Genomic DNA Translation: CAA97715.1
BK006945 Genomic DNA Translation: DAA09453.1
PIRiS64985
RefSeqiNP_013244.1, NM_001182030.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi31412, 74 interactors
DIPiDIP-2618N
IntActiQ12429, 1 interactor
MINTiQ12429
STRINGi4932.YLR143W

PTM databases

iPTMnetiQ12429

Proteomic databases

MaxQBiQ12429
PaxDbiQ12429
PRIDEiQ12429

Genome annotation databases

EnsemblFungiiYLR143W_mRNA; YLR143W; YLR143W
GeneIDi850835
KEGGisce:YLR143W

Organism-specific databases

SGDiS000004133, DPH6
VEuPathDBiFungiDB:YLR143W

Phylogenomic databases

eggNOGiKOG2316, Eukaryota
KOG2317, Eukaryota
GeneTreeiENSGT00420000029820
HOGENOMiCLU_010289_2_1_1
InParanoidiQ12429
OMAiSNSFGRH

Enzyme and pathway databases

UniPathwayiUPA00559
BioCyciMetaCyc:MONOMER-17855
BRENDAi6.3.1.14, 984
ReactomeiR-SCE-5358493, Synthesis of diphthamide-EEF2

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q12429
RNActiQ12429, protein

Family and domain databases

CDDicd01994, Alpha_ANH_like_IV, 1 hit
Gene3Di3.30.1330.40, 2 hits
3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR002761, Diphthami_syn_dom
IPR030662, DPH6/MJ0570
IPR014729, Rossmann-like_a/b/a_fold
IPR035959, RutC-like_sf
IPR006175, YjgF/YER057c/UK114
PANTHERiPTHR12196, PTHR12196, 1 hit
PfamiView protein in Pfam
PF01902, Diphthami_syn_2, 1 hit
PF01042, Ribonuc_L-PSP, 1 hit
SUPFAMiSSF55298, SSF55298, 2 hits
TIGRFAMsiTIGR00290, MJ0570_dom, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDPH6_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q12429
Secondary accession number(s): D6VYD7, Q07261
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: February 10, 2021
This is version 150 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families
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