UniProtKB - Q12417 (PRP46_YEAST)
Protein
Pre-mRNA-splicing factor PRP46
Gene
PRP46
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Involved in pre-mRNA splicing. May also be required for cell cycle progression at G2/M (By similarity).By similarity1 Publication
Miscellaneous
Present with 1770 molecules/cell in log phase SD medium.1 Publication
GO - Biological processi
- mRNA splicing, via spliceosome Source: SGD
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-splicing factor PRP46Alternative name(s): Complexed with CEF1 protein 1 PRP nineteen-associated complex protein 50 PRP19-associated complex protein 50 Pre-mRNA-processing protein 46 |
Gene namesi | Name:PRP46 Synonyms:CWC1, NTC50 Ordered Locus Names:YPL151C ORF Names:P2594 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YPL151C |
SGDi | S000006072, PRP46 |
Subcellular locationi
Nucleus
- catalytic step 2 spliceosome Source: GO_Central
- spliceosomal complex Source: SGD
Other locations
- Cul4-RING E3 ubiquitin ligase complex Source: GO_Central
- cytoplasm Source: UniProtKB-SubCell
- Prp19 complex Source: SGD
Keywords - Cellular componenti
Cytoplasm, Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051169 | 1 – 451 | Pre-mRNA-splicing factor PRP46Add BLAST | 451 |
Proteomic databases
MaxQBi | Q12417 |
PaxDbi | Q12417 |
PRIDEi | Q12417 |
2D gel databases
COMPLUYEAST-2DPAGEi | Q12417 |
PTM databases
iPTMneti | Q12417 |
Interactioni
Subunit structurei
Belongs to the CWC complex (or CEF1-associated complex), a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.
Interacts with CEF1, CLF1, NTC20, PRP45 and SYF1.
3 PublicationsBinary interactionsi
Q12417
With | #Exp. | IntAct |
---|---|---|
PRP45 [P28004] | 4 | EBI-710,EBI-20640 |
Protein-protein interaction databases
BioGRIDi | 36032, 225 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex |
DIPi | DIP-3994N |
IntActi | Q12417, 45 interactors |
MINTi | Q12417 |
STRINGi | 4932.YPL151C |
Miscellaneous databases
RNActi | Q12417, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q12417 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 137 – 168 | WD 1Add BLAST | 32 | |
Repeati | 180 – 210 | WD 2Add BLAST | 31 | |
Repeati | 222 – 252 | WD 3Add BLAST | 31 | |
Repeati | 264 – 294 | WD 4Add BLAST | 31 | |
Repeati | 306 – 335 | WD 5Add BLAST | 30 | |
Repeati | 348 – 377 | WD 6Add BLAST | 30 | |
Repeati | 397 – 427 | WD 7Add BLAST | 31 |
Sequence similaritiesi
Belongs to the WD repeat PRL1/PRL2 family.Curated
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0285, Eukaryota |
GeneTreei | ENSGT00940000155316 |
HOGENOMi | CLU_000288_72_0_1 |
InParanoidi | Q12417 |
OMAi | FAMCFDQ |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 5 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 4 hits PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12417-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDGNDHKVEN LGDVDKFYSR IRWNNQFSYM ATLPPHLQSE MEGQKSLLMR
60 70 80 90 100
YDTYRKESSS FSGEGKKVTL QHVPTDFSEA SQAVISKKDH DTHASAFVNK
110 120 130 140 150
IFQPEVAEEL IVNRYEKLLS QRPEWHAPWK LSRVINGHLG WVRCVAIDPV
160 170 180 190 200
DNEWFITGSN DTTMKVWDLA TGKLKTTLAG HVMTVRDVAV SDRHPYLFSV
210 220 230 240 250
SEDKTVKCWD LEKNQIIRDY YGHLSGVRTV SIHPTLDLIA TAGRDSVIKL
260 270 280 290 300
WDMRTRIPVI TLVGHKGPIN QVQCTPVDPQ VVSSSTDATV RLWDVVAGKT
310 320 330 340 350
MKVLTHHKRS VRATALHPKE FSVASACTDD IRSWGLAEGS LLTNFESEKT
360 370 380 390 400
GIINTLSINQ DDVLFAGGDN GVLSFYDYKS GHKYQSLATR EMVGSLEGER
410 420 430 440 450
SVLCSTFDKT GLRLITGEAD KSIKIWKQDE TATKESEPGL AWNPNLSAKR
F
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X96770 Genomic DNA Translation: CAA65570.1 Z73507 Genomic DNA Translation: CAA97856.1 BK006949 Genomic DNA Translation: DAA11284.1 |
PIRi | S65162 |
RefSeqi | NP_015174.1, NM_001183965.1 |
Genome annotation databases
EnsemblFungii | YPL151C_mRNA; YPL151C; YPL151C |
GeneIDi | 855952 |
KEGGi | sce:YPL151C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X96770 Genomic DNA Translation: CAA65570.1 Z73507 Genomic DNA Translation: CAA97856.1 BK006949 Genomic DNA Translation: DAA11284.1 |
PIRi | S65162 |
RefSeqi | NP_015174.1, NM_001183965.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5GM6 | electron microscopy | 3.50 | O | 1-451 | [»] | |
5GMK | electron microscopy | 3.40 | O | 1-451 | [»] | |
5LJ3 | electron microscopy | 3.80 | J | 1-451 | [»] | |
5LJ5 | electron microscopy | 3.80 | J | 1-451 | [»] | |
5LQW | electron microscopy | 5.80 | K | 1-451 | [»] | |
5MPS | electron microscopy | 3.85 | J | 1-451 | [»] | |
5MQ0 | electron microscopy | 4.17 | J | 1-451 | [»] | |
5WSG | electron microscopy | 4.00 | O | 1-451 | [»] | |
5Y88 | electron microscopy | 3.70 | O | 1-451 | [»] | |
5YLZ | electron microscopy | 3.60 | O | 1-451 | [»] | |
6BK8 | electron microscopy | 3.30 | D | 1-451 | [»] | |
6EXN | electron microscopy | 3.70 | J | 1-451 | [»] | |
6J6G | electron microscopy | 3.20 | O | 1-451 | [»] | |
6J6H | electron microscopy | 3.60 | O | 1-451 | [»] | |
6J6N | electron microscopy | 3.86 | O | 1-451 | [»] | |
6J6Q | electron microscopy | 3.70 | O | 1-451 | [»] | |
SMRi | Q12417 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36032, 225 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex |
DIPi | DIP-3994N |
IntActi | Q12417, 45 interactors |
MINTi | Q12417 |
STRINGi | 4932.YPL151C |
PTM databases
iPTMneti | Q12417 |
2D gel databases
COMPLUYEAST-2DPAGEi | Q12417 |
Proteomic databases
MaxQBi | Q12417 |
PaxDbi | Q12417 |
PRIDEi | Q12417 |
Genome annotation databases
EnsemblFungii | YPL151C_mRNA; YPL151C; YPL151C |
GeneIDi | 855952 |
KEGGi | sce:YPL151C |
Organism-specific databases
EuPathDBi | FungiDB:YPL151C |
SGDi | S000006072, PRP46 |
Phylogenomic databases
eggNOGi | KOG0285, Eukaryota |
GeneTreei | ENSGT00940000155316 |
HOGENOMi | CLU_000288_72_0_1 |
InParanoidi | Q12417 |
OMAi | FAMCFDQ |
Miscellaneous databases
PROi | PR:Q12417 |
RNActi | Q12417, protein |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 5 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 4 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRP46_YEAST | |
Accessioni | Q12417Primary (citable) accession number: Q12417 Secondary accession number(s): D6W3L8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 197 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD