UniProtKB - Q12380 (ATG5_YEAST)
Autophagy protein 5
ATG5
Functioni
Involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy). Also required for mitophagy, which eliminates defective or superfluous mitochondria in order to fulfill cellular energy requirements and prevent excess ROS production. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 and ATG8 association to the vesicle membranes. ATG12-ATG5 rearranges the ATG3 catalytic center and enhances its E2 activity. Plays a role in the regulation of filamentous growth and chronological longevity.
17 PublicationsMiscellaneous
GO - Molecular functioni
- cargo receptor ligand activity Source: SGD
- enzyme activator activity Source: SGD
- hydrolase activity Source: UniProtKB-KW
GO - Biological processi
- autophagosome assembly Source: GO_Central
- autophagy of mitochondrion Source: SGD
- autophagy of nucleus Source: GO_Central
- cellular response to nitrogen starvation Source: GO_Central
- C-terminal protein lipidation Source: SGD
- cytoplasm to vacuole transport by the Cvt pathway Source: SGD
- late nucleophagy Source: SGD
- macroautophagy Source: ComplexPortal
- piecemeal microautophagy of the nucleus Source: SGD
- protein lipidation Source: ComplexPortal
- selective autophagy Source: SGD
Keywordsi
Molecular function | Hydrolase |
Biological process | Autophagy, Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-SCE-1632852, Macroautophagy R-SCE-8934903, Receptor Mediated Mitophagy |
Names & Taxonomyi
Protein namesi | Recommended name: Autophagy protein 5 |
Gene namesi | Name:ATG5 Synonyms:APG5 Ordered Locus Names:YPL149W ORF Names:P2601 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006070, ATG5 |
VEuPathDBi | FungiDB:YPL149W |
Subcellular locationi
Other locations
- Preautophagosomal structure membrane 6 Publications; Peripheral membrane protein 6 Publications
Note: Localizes to the isolation membrane (IM), a membrane sac which is generated from the pre-autophagosomal structure (PAS). Ultimately, the IM expands to become a mature autophagosome. Localizes also to a dot at the junction between the IM and the vacuolar membrane, termed the vacuole-IM contact site (VICS). Correct localization to the PAS requires ATG21.
Cytosol
- cytosol Source: SGD
Nucleus
- nucleus Source: SGD
Vacuole
- autophagosome Source: SGD
Other locations
- Atg12-Atg5-Atg16 complex Source: SGD
- membrane Source: SGD
- phagophore Source: SGD
- phagophore assembly site Source: SGD
- phagophore assembly site membrane Source: GO_Central
- transferase complex Source: ComplexPortal
- vacuole-isolation membrane contact site Source: SGD
Keywords - Cellular componenti
MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 149 | K → R: Loss of conjugation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219010 | 1 – 294 | Autophagy protein 5Add BLAST | 294 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 149 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12) |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | Q12380 |
PaxDbi | Q12380 |
PRIDEi | Q12380 |
Interactioni
Subunit structurei
Conjugated with ATG12. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. The ATG12-ATG5 conjugate associates also with ATG3.
2 PublicationsBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 36034, 169 interactors |
ComplexPortali | CPX-1848, ATG12-ATG5 complex CPX-1849, ATG12-ATG5-ATG16 complex |
DIPi | DIP-1195N |
IntActi | Q12380, 30 interactors |
MINTi | Q12380 |
STRINGi | 4932.YPL149W |
Miscellaneous databases
RNActi | Q12380, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q12380 |
SMRi | Q12380 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q12380 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2976, Eukaryota |
GeneTreei | ENSGT00390000004766 |
HOGENOMi | CLU_051894_2_0_1 |
InParanoidi | Q12380 |
OMAi | KWHYPLG |
Family and domain databases
Gene3Di | 1.10.246.190, 1 hit 3.10.20.620, 1 hit |
IDEALi | IID50256 |
InterProi | View protein in InterPro IPR007239, Atg5 IPR042526, Atg5_HR IPR042527, Atg5_UblA_dom |
PANTHERi | PTHR13040, PTHR13040, 1 hit |
Pfami | View protein in Pfam PF04106, APG5, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MNDIKQLLWN GELNVLVSID PSFLMKGSPR EIAVLRIRVP RETYLVNYMP
60 70 80 90 100
LIWNKIKSFL SFDPLTDSEK YFWFEHNKTP IPWNYPVGVL FDCLAGKSAT
110 120 130 140 150
FTTSFENQVK DVLTFLRIHL VMGDSLPPTI IPIASSKTQA EKFWFHQWKQ
160 170 180 190 200
VCFILNGSSK AIMSLSVNEA RKFWGSVITR NFQDFIEISN KISSSRPRHI
210 220 230 240 250
PLIIQTSRTS GTFRISQPTI SMTGVNPTLK DIEGDILDVK EGINGNDVMV
260 270 280 290
ICQGIEIPWH MLLYDLYSKL RSFDGFLYIT LVPIKGGDKA SSEL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83519 Genomic DNA Translation: BAA11937.1 X96770 Genomic DNA Translation: CAA65572.1 Z73505 Genomic DNA Translation: CAA97854.1 BK006949 Genomic DNA Translation: DAA11286.1 |
PIRi | S65160 |
RefSeqi | NP_015176.1, NM_001183963.1 |
Genome annotation databases
EnsemblFungii | YPL149W_mRNA; YPL149W; YPL149W |
GeneIDi | 855954 |
KEGGi | sce:YPL149W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83519 Genomic DNA Translation: BAA11937.1 X96770 Genomic DNA Translation: CAA65572.1 Z73505 Genomic DNA Translation: CAA97854.1 BK006949 Genomic DNA Translation: DAA11286.1 |
PIRi | S65160 |
RefSeqi | NP_015176.1, NM_001183963.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DYM | X-ray | 2.20 | A/C/E/G | 1-294 | [»] | |
2DYO | X-ray | 1.97 | A | 1-294 | [»] | |
3W1S | X-ray | 2.60 | A | 1-284 | [»] | |
AlphaFoldDBi | Q12380 | |||||
SMRi | Q12380 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36034, 169 interactors |
ComplexPortali | CPX-1848, ATG12-ATG5 complex CPX-1849, ATG12-ATG5-ATG16 complex |
DIPi | DIP-1195N |
IntActi | Q12380, 30 interactors |
MINTi | Q12380 |
STRINGi | 4932.YPL149W |
Proteomic databases
MaxQBi | Q12380 |
PaxDbi | Q12380 |
PRIDEi | Q12380 |
Genome annotation databases
EnsemblFungii | YPL149W_mRNA; YPL149W; YPL149W |
GeneIDi | 855954 |
KEGGi | sce:YPL149W |
Organism-specific databases
SGDi | S000006070, ATG5 |
VEuPathDBi | FungiDB:YPL149W |
Phylogenomic databases
eggNOGi | KOG2976, Eukaryota |
GeneTreei | ENSGT00390000004766 |
HOGENOMi | CLU_051894_2_0_1 |
InParanoidi | Q12380 |
OMAi | KWHYPLG |
Enzyme and pathway databases
Reactomei | R-SCE-1632852, Macroautophagy R-SCE-8934903, Receptor Mediated Mitophagy |
Miscellaneous databases
EvolutionaryTracei | Q12380 |
PROi | PR:Q12380 |
RNActi | Q12380, protein |
Family and domain databases
Gene3Di | 1.10.246.190, 1 hit 3.10.20.620, 1 hit |
IDEALi | IID50256 |
InterProi | View protein in InterPro IPR007239, Atg5 IPR042526, Atg5_HR IPR042527, Atg5_UblA_dom |
PANTHERi | PTHR13040, PTHR13040, 1 hit |
Pfami | View protein in Pfam PF04106, APG5, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATG5_YEAST | |
Accessioni | Q12380Primary (citable) accession number: Q12380 Secondary accession number(s): D6W3M0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families