UniProtKB - Q12373 (HIF1_YEAST)
HAT1-interacting factor 1
HIF1
Functioni
Histone H3 and H4 specific chaperone component of the nuclear histone acetyltransferase B (HAT-B) complex. Involved in chromatin assembly and telomere silencing.
2 PublicationsMiscellaneous
GO - Molecular functioni
- histone binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- histone acetylation Source: SGD
- nucleosome assembly Source: SGD
- subtelomeric heterochromatin assembly Source: SGD
Keywordsi
Molecular function | Chromatin regulator |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: HAT1-interacting factor 1 |
Gene namesi | Name:HIF1 Ordered Locus Names:YLL022C ORF Names:L1205 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003945, HIF1 |
VEuPathDBi | FungiDB:YLL022C |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Nucleus
- histone acetyltransferase complex Source: SGD
- nucleus Source: SGD
Other locations
- chromosome, telomeric region Source: GOC
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 80 – 158 | Missing : Abolishes interaction with heterotetrameric histone H3 and H4 and with dimeric histone H2A and H2B. 1 PublicationAdd BLAST | 79 | |
Mutagenesisi | 85 – 198 | Missing : Abolishes interaction with histones H2A, H2B, H3 and H4. 2 PublicationsAdd BLAST | 114 | |
Mutagenesisi | 95 – 158 | Missing : Mildly decreases interaction with heterotetrameric histone H3 and H4 and abolishes interaction with dimeric histone H2A and H2B. 1 PublicationAdd BLAST | 64 | |
Mutagenesisi | 135 – 158 | Missing : Minimal decrease of interaction with heterotetrameric histone H3 and H4 and with dimeric histone H2A and H2B. 2 PublicationsAdd BLAST | 24 | |
Mutagenesisi | 248 | E → A: Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-250; A-288; A-291 and 332-A--A-342. 1 Publication | 1 | |
Mutagenesisi | 250 | E → A: Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-248; A-288; A-291 and 332-A--A-342. 1 Publication | 1 | |
Mutagenesisi | 288 | D → A: Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-248; A-250; A-291 and 332-A--A-342. 1 Publication | 1 | |
Mutagenesisi | 291 | R → A: Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-248; A-250; A-288 and 332-A--A-342. 1 Publication | 1 | |
Mutagenesisi | 332 – 342 | QIQDDIDEVQE → AIQAAIDAVQA: Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-248; A-250; A-288 and A-291. 1 PublicationAdd BLAST | 11 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000227731 | 1 – 385 | HAT1-interacting factor 1Add BLAST | 385 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 174 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q12373 |
PaxDbi | Q12373 |
PRIDEi | Q12373 |
PTM databases
iPTMneti | Q12373 |
Interactioni
Subunit structurei
Homodimer (PubMed:27618665). The homodimer interacts with a histone tetramer containing H3 and H4; the interaction is direct (PubMed:24946827, PubMed:27618665). The homodimer interacts with heterodimeric histone H2A and H2B; the interaction is direct (PubMed:24946827, PubMed:27618665).
Component of the nuclear histone acetyltransferase B (HAT-B) complex composed of at least HAT1, HAT2 and HIF1 (PubMed:14761951, PubMed:15099519). Does not interact with HAT1 in the absence of HAT2 (PubMed:14761951, PubMed:24946827).
Interacts with histones H3 and H4 in a HAT1/HAT2 dependent manner (PubMed:14761951, PubMed:15099519). Interaction with heterotetrameric histone H3 and H4 precludes interaction with dimeric histone H2A and H2B, irrespective of the fact that their binding involves non-identical regions of the protein (PubMed:24946827, PubMed:27618665).
4 PublicationsBinary interactionsi
Q12373
With | #Exp. | IntAct |
---|---|---|
HAT2 [P39984] | 4 | EBI-31911,EBI-8185 |
HHF2 [P02309] | 5 | EBI-31911,EBI-8113 |
GO - Molecular functioni
- histone binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 31231, 58 interactors |
ComplexPortali | CPX-1682, Histone acetyltransferase B |
DIPi | DIP-6442N |
IntActi | Q12373, 6 interactors |
MINTi | Q12373 |
STRINGi | 4932.YLL022C |
Miscellaneous databases
RNActi | Q12373, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q12373 |
SMRi | Q12373 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 186 – 220 | TPR 1Sequence analysisAdd BLAST | 35 | |
Repeati | 229 – 262 | TPR 2Sequence analysisAdd BLAST | 34 | |
Repeati | 289 – 322 | TPR 3Sequence analysisAdd BLAST | 34 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 80 – 199 | Important for interaction with heterotetrameric histone H3 and H4 and for interaction with dimeric histone H2A and H2B2 PublicationsAdd BLAST | 120 | |
Regioni | 85 – 163 | DisorderedSequence analysisAdd BLAST | 79 | |
Regioni | 248 – 332 | Interaction with dimeric histone H2A and H2B1 PublicationAdd BLAST | 85 | |
Regioni | 340 – 385 | DisorderedSequence analysisAdd BLAST | 46 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 109 – 125 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 132 – 160 | Acidic residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 346 – 360 | Polar residuesSequence analysisAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, TPR repeatPhylogenomic databases
eggNOGi | KOG4563, Eukaryota |
HOGENOMi | CLU_797425_0_0_1 |
InParanoidi | Q12373 |
OMAi | TIFAQAV |
Family and domain databases
Gene3Di | 1.25.40.10, 2 hits |
IDEALi | IID50290 |
InterProi | View protein in InterPro IPR011990, TPR-like_helical_dom_sf |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKLRAEDVLA NGTSRHKVQI DMERQVQIAK DLLAQKKFLE AAKRCQQTLD
60 70 80 90 100
SLPKDGLLPD PELFTIFAQA VYNMEVQNSG NLFGDALLAG DDGSGSESES
110 120 130 140 150
EPESDVSNGE EGNENGQTEI PNSRMFQFDQ EEEDLTGDVD SGDSEDSGEG
160 170 180 190 200
SEEEEENVEK EEERLALHEL ANFSPANEHD DEIEDVSQLR KSGFHIYFEN
210 220 230 240 250
DLYENALDLL AQALMLLGRP TADGQSLTEN SRLRIGDVYI LMGDIEREAE
260 270 280 290 300
MFSRAIHHYL KALGYYKTLK PAEQVTEKVI QAEFLVCDAL RWVDQVPAKD
310 320 330 340 350
KLKRFKHAKA LLEKHMTTRP KDSELQQARL AQIQDDIDEV QENQQHGSKR
360 370 380
PLSQPTTSIG FPALEKPLGD FNDLSQLVKK KPRRH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97560 Genomic DNA Translation: CAA66169.1 Z73127 Genomic DNA Translation: CAA97470.1 BK006945 Genomic DNA Translation: DAA09298.1 |
PIRi | S64770 |
RefSeqi | NP_013078.1, NM_001181842.1 |
Genome annotation databases
EnsemblFungii | YLL022C_mRNA; YLL022C; YLL022C |
GeneIDi | 850638 |
KEGGi | sce:YLL022C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97560 Genomic DNA Translation: CAA66169.1 Z73127 Genomic DNA Translation: CAA97470.1 BK006945 Genomic DNA Translation: DAA09298.1 |
PIRi | S64770 |
RefSeqi | NP_013078.1, NM_001181842.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4NQ0 | X-ray | 2.10 | A | 1-385 | [»] | |
5BT1 | X-ray | 2.62 | A/B | 1-385 | [»] | |
AlphaFoldDBi | Q12373 | |||||
SMRi | Q12373 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31231, 58 interactors |
ComplexPortali | CPX-1682, Histone acetyltransferase B |
DIPi | DIP-6442N |
IntActi | Q12373, 6 interactors |
MINTi | Q12373 |
STRINGi | 4932.YLL022C |
PTM databases
iPTMneti | Q12373 |
Proteomic databases
MaxQBi | Q12373 |
PaxDbi | Q12373 |
PRIDEi | Q12373 |
Genome annotation databases
EnsemblFungii | YLL022C_mRNA; YLL022C; YLL022C |
GeneIDi | 850638 |
KEGGi | sce:YLL022C |
Organism-specific databases
SGDi | S000003945, HIF1 |
VEuPathDBi | FungiDB:YLL022C |
Phylogenomic databases
eggNOGi | KOG4563, Eukaryota |
HOGENOMi | CLU_797425_0_0_1 |
InParanoidi | Q12373 |
OMAi | TIFAQAV |
Miscellaneous databases
PROi | PR:Q12373 |
RNActi | Q12373, protein |
Family and domain databases
Gene3Di | 1.25.40.10, 2 hits |
IDEALi | IID50290 |
InterProi | View protein in InterPro IPR011990, TPR-like_helical_dom_sf |
MobiDBi | Search... |
Entry informationi
Entry namei | HIF1_YEAST | |
Accessioni | Q12373Primary (citable) accession number: Q12373 Secondary accession number(s): D6VXY2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 21, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families