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UniProtKB - Q12362 (RIB2_YEAST)
Protein
Bifunctional protein RIB2
Gene
RIB2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Responsible for synthesis of pseudouridine from uracil-32 in cytoplasmic transfer RNAs.
Involved in riboflavin biosynthesis. Converts 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1H,3H)-pyrimidinedione 5'-phosphate.
Miscellaneous
Present with 1540 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:5.4.99.281 Publication
- 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H+ + H2O = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH4+1 PublicationEC:3.5.4.261 Publication
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion.By similarity
: riboflavin biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP. This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 211 | By similarity | 1 | |
Metal bindingi | 482 | Zinc; catalyticBy similarity | 1 | |
Active sitei | 484 | Proton donorBy similarity | 1 | |
Metal bindingi | 515 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 525 | Zinc; catalyticBy similarity | 1 |
GO - Molecular functioni
- 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate deaminase activity Source: SGD
- diaminohydroxyphosphoribosylaminopyrimidine deaminase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- pseudouridine synthase activity Source: SGD
- RNA binding Source: UniProtKB-KW
- tRNA pseudouridine synthase activity Source: UniProtKB-EC
GO - Biological processi
- enzyme-directed rRNA pseudouridine synthesis Source: GO_Central
- riboflavin biosynthetic process Source: SGD
- tRNA pseudouridine synthesis Source: SGD
Keywordsi
Molecular function | Hydrolase, Isomerase, RNA-binding |
Biological process | Riboflavin biosynthesis, tRNA processing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 5.4.99.28, 984 |
UniPathwayi | UPA00275;UER00401 |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional protein RIB2Including the following 2 domains: tRNA pseudouridine(32) synthase, cytoplasmic (EC:5.4.99.28) Alternative name(s): tRNA pseudouridine synthase 8 tRNA pseudouridylate synthase 8 tRNA-uridine isomerase 8 Alternative name(s): Riboflavin-specific deaminase |
Gene namesi | Name:RIB2 Synonyms:PUS8 Ordered Locus Names:YOL066C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005427, RIB2 |
VEuPathDBi | FungiDB:YOL066C |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000162725 | 1 – 591 | Bifunctional protein RIB2Add BLAST | 591 |
Proteomic databases
MaxQBi | Q12362 |
PaxDbi | Q12362 |
PRIDEi | Q12362 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 34335, 175 interactors |
DIPi | DIP-3840N |
STRINGi | 4932.YOL066C |
Miscellaneous databases
RNActi | Q12362, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 99 – 168 | S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST | 70 | |
Domaini | 433 – 552 | CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST | 120 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 432 | tRNA pseudouridine synthaseAdd BLAST | 432 | |
Regioni | 433 – 591 | DRAP deaminaseAdd BLAST | 159 |
Sequence similaritiesi
In the N-terminal section; belongs to the pseudouridine synthase RluA family.Curated
In the C-terminal section; belongs to the cytidine and deoxycytidylate deaminase family.Curated
Phylogenomic databases
eggNOGi | KOG1018, Eukaryota KOG1919, Eukaryota |
GeneTreei | ENSGT00420000029802 |
HOGENOMi | CLU_016902_12_4_1 |
InParanoidi | Q12362 |
OMAi | RYNTITK |
Family and domain databases
InterProi | View protein in InterPro IPR002125, CMP_dCMP_dom IPR016193, Cytidine_deaminase-like IPR020103, PsdUridine_synth_cat_dom_sf IPR006225, PsdUridine_synth_RluC/D IPR006224, PsdUridine_synth_RluC/D_CS IPR006145, PsdUridine_synth_RsuA/RluA |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit |
SUPFAMi | SSF53927, SSF53927, 1 hit SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00005, rluA_subfam, 1 hit |
PROSITEi | View protein in PROSITE PS51747, CYT_DCMP_DEAMINASES_2, 1 hit PS01129, PSI_RLU, 1 hit PS50889, S4, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12362-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEDSNNEASD DFNNLLNKEI ESAKEVKLRK FANRNNNRNE NSSKVKDASG
60 70 80 90 100
FRLRVIQTDG HKTKKTDPDY EVTIDGPLRK IEPYFFTYKT FCKERWRDRK
110 120 130 140 150
LVDVFVSEFR DREPSYYSKT IAEGKVYLND EPANLDTIIR DGDLITHKVH
160 170 180 190 200
RHEPPVTSKP IDIVFEDEDI LVIDKPSSIP VHPTGRYRFN TITKMLERQL
210 220 230 240 250
GYSVHPCNRL DKPTSGLMFL AKTPLGADRM GDQMKAREVT KEYVARVKGE
260 270 280 290 300
FPIGIVEVDK PVRSVNPKVA LNAVCEMSDE NAKHAKTVFQ RVSYDGQTSI
310 320 330 340 350
VKCKPLTGRT HQIRVHLQYL GFPIANDPIY SNPDIWGPDL GRGGLQNYDD
360 370 380 390 400
IVLKLDAIGK TNPAESWIHP HSEGEYLLGR QCEECEAEMY TDPGTNDLDL
410 420 430 440 450
WLHAFRYESL ERNSDTQKPL WSYRTKYPEW ALEPHRRYME MAVKEAGKCG
460 470 480 490 500
PTKTAFSVGA VLVHGTQVLA TGYSRELPGN THAEQCALIK YSQLHPNCPT
510 520 530 540 550
IVPMGTVLYT TMEPCSFRLS GNEPCCDRIL ATQGAIGTVF VGVMEPDTFV
560 570 580 590
KNNTSLNKLE SHGVNYIQIP GYEEECTIIA FKGHDNSDDK A
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z21618 Genomic DNA Translation: CAA79742.1 Z74808 Genomic DNA Translation: CAA99076.1 BK006948 Genomic DNA Translation: DAA10717.1 |
PIRi | S50972 |
RefSeqi | NP_014575.1, NM_001183321.1 |
Genome annotation databases
EnsemblFungii | YOL066C_mRNA; YOL066C; YOL066C |
GeneIDi | 854088 |
KEGGi | sce:YOL066C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z21618 Genomic DNA Translation: CAA79742.1 Z74808 Genomic DNA Translation: CAA99076.1 BK006948 Genomic DNA Translation: DAA10717.1 |
PIRi | S50972 |
RefSeqi | NP_014575.1, NM_001183321.1 |
3D structure databases
SMRi | Q12362 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34335, 175 interactors |
DIPi | DIP-3840N |
STRINGi | 4932.YOL066C |
Proteomic databases
MaxQBi | Q12362 |
PaxDbi | Q12362 |
PRIDEi | Q12362 |
Genome annotation databases
EnsemblFungii | YOL066C_mRNA; YOL066C; YOL066C |
GeneIDi | 854088 |
KEGGi | sce:YOL066C |
Organism-specific databases
SGDi | S000005427, RIB2 |
VEuPathDBi | FungiDB:YOL066C |
Phylogenomic databases
eggNOGi | KOG1018, Eukaryota KOG1919, Eukaryota |
GeneTreei | ENSGT00420000029802 |
HOGENOMi | CLU_016902_12_4_1 |
InParanoidi | Q12362 |
OMAi | RYNTITK |
Enzyme and pathway databases
UniPathwayi | UPA00275;UER00401 |
BRENDAi | 5.4.99.28, 984 |
Miscellaneous databases
PROi | PR:Q12362 |
RNActi | Q12362, protein |
Family and domain databases
InterProi | View protein in InterPro IPR002125, CMP_dCMP_dom IPR016193, Cytidine_deaminase-like IPR020103, PsdUridine_synth_cat_dom_sf IPR006225, PsdUridine_synth_RluC/D IPR006224, PsdUridine_synth_RluC/D_CS IPR006145, PsdUridine_synth_RsuA/RluA |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit |
SUPFAMi | SSF53927, SSF53927, 1 hit SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00005, rluA_subfam, 1 hit |
PROSITEi | View protein in PROSITE PS51747, CYT_DCMP_DEAMINASES_2, 1 hit PS01129, PSI_RLU, 1 hit PS50889, S4, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RIB2_YEAST | |
Accessioni | Q12362Primary (citable) accession number: Q12362 Secondary accession number(s): D6W201 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families