UniProtKB - Q12330 (RUXE_YEAST)
Small nuclear ribonucleoprotein E
SME1
Functioni
Involved in pre-mRNA splicing. Binds and is required for the stability of snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site. Involved in cap modification.
1 PublicationMiscellaneous
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
- splicing factor binding Source: SGD
GO - Biological processi
- 7-methylguanosine cap hypermethylation Source: ComplexPortal
- mRNA 5'-splice site recognition Source: ComplexPortal
- mRNA splicing, via spliceosome Source: SGD
- spliceosomal complex assembly Source: ComplexPortal
- spliceosomal snRNP assembly Source: GO_Central
- U2-type prespliceosome assembly Source: ComplexPortal
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Names & Taxonomyi
Protein namesi | Recommended name: Small nuclear ribonucleoprotein EShort name: snRNP-E Alternative name(s): Sm protein E Short name: Sm-E Short name: SmE |
Gene namesi | Name:SME1 Ordered Locus Names:YOR159C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005685, SME1 |
VEuPathDBi | FungiDB:YOR159C |
Subcellular locationi
Nucleus
- commitment complex Source: ComplexPortal
- nucleus Source: ComplexPortal
- precatalytic spliceosome Source: GO_Central
- spliceosomal complex Source: ComplexPortal
- U1 snRNP Source: SGD
- U2 snRNP Source: GO_Central
- U2-type prespliceosome Source: SGD
- U4 snRNP Source: GO_Central
- U4/U6 snRNP Source: ComplexPortal
- U4/U6 x U5 tri-snRNP complex Source: SGD
- U5 snRNP Source: SGD
Other locations
- cytoplasm Source: ComplexPortal
Keywords - Cellular componenti
Cytoplasm, Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000125535 | 1 – 94 | Small nuclear ribonucleoprotein EAdd BLAST | 94 |
Proteomic databases
MaxQBi | Q12330 |
PaxDbi | Q12330 |
PRIDEi | Q12330 |
PTM databases
iPTMneti | Q12330 |
Interactioni
Subunit structurei
Component of the Sm core complex, present in spliceosomal snRNP U1, U2, U4/U6 and U5. The core complex contains SMB1, SMD1, SMD2, SMD3, SME1, SMX3 and SMX2 (Sm proteins B, D1, D2, D3, E, F and G, respectively), and is probably a heptameric ring structure. SME1 specifically interacts with SMX2 and SMX3.
Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.
3 PublicationsBinary interactionsi
Q12330
With | #Exp. | IntAct |
---|---|---|
SMX2 [P40204] | 5 | EBI-16359,EBI-637 |
SMX3 [P54999] | 6 | EBI-16359,EBI-770 |
GO - Molecular functioni
- splicing factor binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 34555, 157 interactors |
ComplexPortali | CPX-23, U1 small nuclear ribonucleoprotein complex CPX-25, U4/U6.U5 tri-small nuclear ribonucleoprotein complex CPX-26, U2 small nuclear ribonucleoprotein complex CPX-29, U5 small nuclear ribonucleoprotein complex CPX-30, U5 small nuclear ribonucleoprotein complex, AAR2 variant CPX-31, U4 small nuclear ribonucleoprotein complex CPX-32, U4/U6 small nuclear ribonucleoprotein complex CPX-43, Sm complex |
DIPi | DIP-1633N |
IntActi | Q12330, 16 interactors |
MINTi | Q12330 |
STRINGi | 4932.YOR159C |
Miscellaneous databases
RNActi | Q12330, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q12330 |
SMRi | Q12330 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1774, Eukaryota |
GeneTreei | ENSGT00390000012818 |
HOGENOMi | CLU_125186_1_0_1 |
InParanoidi | Q12330 |
OMAi | TIWLYEQ |
Family and domain databases
CDDi | cd01718, Sm_E, 1 hit |
InterProi | View protein in InterPro IPR001163, LSM_dom_euk/arc IPR010920, LSM_dom_sf IPR027078, snRNP-E |
PANTHERi | PTHR11193, PTHR11193, 1 hit |
Pfami | View protein in Pfam PF01423, LSM, 1 hit |
SMARTi | View protein in SMART SM00651, Sm, 1 hit |
SUPFAMi | SSF50182, SSF50182, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSNKVKTKAM VPPINCIFNF LQQQTPVTIW LFEQIGIRIK GKIVGFDEFM
60 70 80 90
NVVIDEAVEI PVNSADGKED VEKGTPLGKI LLKGDNITLI TSAD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92449 Genomic DNA Translation: CAA63198.1 U55020 Genomic DNA Translation: AAC49645.1 Z75067 Genomic DNA Translation: CAA99365.1 AY558029 Genomic DNA Translation: AAS56355.1 BK006948 Genomic DNA Translation: DAA10932.1 |
PIRi | S67047 |
RefSeqi | NP_014802.3, NM_001183578.3 |
Genome annotation databases
EnsemblFungii | YOR159C_mRNA; YOR159C; YOR159C |
GeneIDi | 854330 |
KEGGi | sce:YOR159C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92449 Genomic DNA Translation: CAA63198.1 U55020 Genomic DNA Translation: AAC49645.1 Z75067 Genomic DNA Translation: CAA99365.1 AY558029 Genomic DNA Translation: AAS56355.1 BK006948 Genomic DNA Translation: DAA10932.1 |
PIRi | S67047 |
RefSeqi | NP_014802.3, NM_001183578.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3JCM | electron microscopy | 3.80 | V/Y | 1-94 | [»] | |
5GAM | electron microscopy | 3.70 | e | 1-94 | [»] | |
5GAN | electron microscopy | 3.60 | e/p | 1-94 | [»] | |
5GAO | electron microscopy | 3.60 | p | 1-94 | [»] | |
5GM6 | electron microscopy | 3.50 | i | 1-94 | [»] | |
5GMK | electron microscopy | 3.40 | i/u | 1-94 | [»] | |
5LJ3 | electron microscopy | 3.80 | e/p | 1-94 | [»] | |
5LJ5 | electron microscopy | 3.80 | e/p | 1-94 | [»] | |
5LQW | electron microscopy | 5.80 | e | 1-94 | [»] | |
5MPS | electron microscopy | 3.85 | e | 1-94 | [»] | |
5MQ0 | electron microscopy | 4.17 | e/p | 1-94 | [»] | |
5NRL | electron microscopy | 7.20 | e/p/w | 1-94 | [»] | |
5WSG | electron microscopy | 4.00 | G/i | 1-94 | [»] | |
5Y88 | electron microscopy | 3.70 | b/i | 1-94 | [»] | |
5YLZ | electron microscopy | 3.60 | b/i | 1-94 | [»] | |
5ZWM | electron microscopy | 3.40 | T/e/i | 1-94 | [»] | |
5ZWN | electron microscopy | 3.30 | e | 1-94 | [»] | |
5ZWO | electron microscopy | 3.90 | T/e/i | 1-94 | [»] | |
6BK8 | electron microscopy | 3.30 | c/l | 1-94 | [»] | |
6EXN | electron microscopy | 3.70 | e/p | 1-94 | [»] | |
6G90 | electron microscopy | 4.00 | e/w | 1-94 | [»] | |
6J6G | electron microscopy | 3.20 | i/u | 1-94 | [»] | |
6J6H | electron microscopy | 3.60 | i/u | 1-94 | [»] | |
6J6N | electron microscopy | 3.86 | i/u | 1-94 | [»] | |
6J6Q | electron microscopy | 3.70 | i/u | 1-94 | [»] | |
6N7P | electron microscopy | 3.60 | O | 1-94 | [»] | |
6N7R | electron microscopy | 3.20 | O | 1-94 | [»] | |
6N7X | electron microscopy | 3.60 | O | 1-94 | [»] | |
7B9V | electron microscopy | 2.80 | e/p | 1-94 | [»] | |
7OQB | electron microscopy | 9.00 | w | 1-93 | [»] | |
7OQC | electron microscopy | 4.10 | e | 1-94 | [»] | |
7OQE | electron microscopy | 5.90 | e/w | 1-94 | [»] | |
AlphaFoldDBi | Q12330 | |||||
SMRi | Q12330 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34555, 157 interactors |
ComplexPortali | CPX-23, U1 small nuclear ribonucleoprotein complex CPX-25, U4/U6.U5 tri-small nuclear ribonucleoprotein complex CPX-26, U2 small nuclear ribonucleoprotein complex CPX-29, U5 small nuclear ribonucleoprotein complex CPX-30, U5 small nuclear ribonucleoprotein complex, AAR2 variant CPX-31, U4 small nuclear ribonucleoprotein complex CPX-32, U4/U6 small nuclear ribonucleoprotein complex CPX-43, Sm complex |
DIPi | DIP-1633N |
IntActi | Q12330, 16 interactors |
MINTi | Q12330 |
STRINGi | 4932.YOR159C |
PTM databases
iPTMneti | Q12330 |
Proteomic databases
MaxQBi | Q12330 |
PaxDbi | Q12330 |
PRIDEi | Q12330 |
Genome annotation databases
EnsemblFungii | YOR159C_mRNA; YOR159C; YOR159C |
GeneIDi | 854330 |
KEGGi | sce:YOR159C |
Organism-specific databases
SGDi | S000005685, SME1 |
VEuPathDBi | FungiDB:YOR159C |
Phylogenomic databases
eggNOGi | KOG1774, Eukaryota |
GeneTreei | ENSGT00390000012818 |
HOGENOMi | CLU_125186_1_0_1 |
InParanoidi | Q12330 |
OMAi | TIWLYEQ |
Miscellaneous databases
PROi | PR:Q12330 |
RNActi | Q12330, protein |
Family and domain databases
CDDi | cd01718, Sm_E, 1 hit |
InterProi | View protein in InterPro IPR001163, LSM_dom_euk/arc IPR010920, LSM_dom_sf IPR027078, snRNP-E |
PANTHERi | PTHR11193, PTHR11193, 1 hit |
Pfami | View protein in Pfam PF01423, LSM, 1 hit |
SMARTi | View protein in SMART SM00651, Sm, 1 hit |
SUPFAMi | SSF50182, SSF50182, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RUXE_YEAST | |
Accessioni | Q12330Primary (citable) accession number: Q12330 Secondary accession number(s): D6W2L6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 184 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families