UniProtKB - Q12328 (TIM22_YEAST)
Mitochondrial import inner membrane translocase subunit TIM22
TIM22
Functioni
Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins, such as mitochondrial carrier family members, into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps. Mediates the insertion of precursor proteins in a 3 step process. After the precursor is tethered to the translocase without losing energy from the Delta(psi), 2 energy-requiring steps are needed. First, Delta(psi) acts on the precursor protein and promotes its docking in the translocase complex. Then, Delta(psi) and an internal signal peptide together induce rapid gating transitions in one pore and closing of the other pore and drive membrane insertion to completion.
3 PublicationsGO - Molecular functioni
- mitochondrion targeting sequence binding Source: SGD
- protein transmembrane transporter activity Source: SGD
GO - Biological processi
- protein insertion into mitochondrial inner membrane Source: ComplexPortal
Keywordsi
Biological process | Protein transport, Translocation, Transport |
Enzyme and pathway databases
Reactomei | R-SCE-1268020, Mitochondrial protein import |
Protein family/group databases
TCDBi | 3.A.8.1.1, the mitochondrial protein translocase (mpt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Mitochondrial import inner membrane translocase subunit TIM22 |
Gene namesi | Name:TIM22 Ordered Locus Names:YDL217C ORF Names:D0884 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002376, TIM22 |
VEuPathDBi | FungiDB:YDL217C |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication2 Publications; Multi-pass membrane protein 2 Publications
Note: Import into inner membrane protein requires TOM20 function.
Cytosol
- cytosol Source: Reactome
Mitochondrion
- mitochondrial inner membrane Source: ComplexPortal
- mitochondrial intermembrane space Source: Reactome
- mitochondrion Source: SGD
- TIM22 mitochondrial import inner membrane insertion complex Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 46 | Mitochondrial intermembrane1 PublicationAdd BLAST | 46 | |
Transmembranei | 47 – 67 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 152 – 172 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 173 – 193 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 194 – 207 | Mitochondrial intermembrane1 PublicationAdd BLAST | 14 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210301 | 1 – 207 | Mitochondrial import inner membrane translocase subunit TIM22Add BLAST | 207 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 42 ↔ 141 | 1 Publication |
Keywords - PTMi
Disulfide bondProteomic databases
MaxQBi | Q12328 |
PaxDbi | Q12328 |
PRIDEi | Q12328 |
Interactioni
Subunit structurei
Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13).
2 PublicationsBinary interactionsi
Q12328
With | #Exp. | IntAct |
---|---|---|
TIM21 [P53220] | 2 | EBI-9132,EBI-23128 |
Protein-protein interaction databases
BioGRIDi | 31828, 579 interactors |
ComplexPortali | CPX-1629, TIM22 mitochondrial inner membrane twin-pore carrier translocase complex |
DIPi | DIP-1142N |
IntActi | Q12328, 6 interactors |
MINTi | Q12328 |
STRINGi | 4932.YDL217C |
Miscellaneous databases
RNActi | Q12328, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3225, Eukaryota |
GeneTreei | ENSGT00390000016067 |
HOGENOMi | CLU_091077_1_0_1 |
InParanoidi | Q12328 |
OMAi | THSYAKN |
Family and domain databases
InterProi | View protein in InterPro IPR039175, TIM22 |
PANTHERi | PTHR14110, PTHR14110, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MVYTGFGLEQ ISPAQKKPYN ELTPEEQGER GAEMIMNFMT SCPGKSVVSG
60 70 80 90 100
VTGFALGGVL GLFMASMAYD TPLHTPTPAN TAATATAGNI GVGGISRTVQ
110 120 130 140 150
QISDLPFRQQ MKLQFTDMGK KSYSSAKNFG YIGMIYAGVE CVIESLRAKN
160 170 180 190 200
DIYNGVTAGF FTGAGLAYKA GPQAALMGGA GFAAFSAAID LYMKSEDGRP
PQNDFKE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X99000 Genomic DNA Translation: CAA67473.1 Z74265 Genomic DNA Translation: CAA98795.1 AY558171 Genomic DNA Translation: AAS56497.1 BK006938 Genomic DNA Translation: DAA11647.1 |
PIRi | S67776 |
RefSeqi | NP_010064.1, NM_001180277.1 |
Genome annotation databases
EnsemblFungii | YDL217C_mRNA; YDL217C; YDL217C |
GeneIDi | 851309 |
KEGGi | sce:YDL217C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X99000 Genomic DNA Translation: CAA67473.1 Z74265 Genomic DNA Translation: CAA98795.1 AY558171 Genomic DNA Translation: AAS56497.1 BK006938 Genomic DNA Translation: DAA11647.1 |
PIRi | S67776 |
RefSeqi | NP_010064.1, NM_001180277.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6LO8 | electron microscopy | 3.83 | A | 1-206 | [»] | |
AlphaFoldDBi | Q12328 | |||||
SMRi | Q12328 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31828, 579 interactors |
ComplexPortali | CPX-1629, TIM22 mitochondrial inner membrane twin-pore carrier translocase complex |
DIPi | DIP-1142N |
IntActi | Q12328, 6 interactors |
MINTi | Q12328 |
STRINGi | 4932.YDL217C |
Protein family/group databases
TCDBi | 3.A.8.1.1, the mitochondrial protein translocase (mpt) family |
Proteomic databases
MaxQBi | Q12328 |
PaxDbi | Q12328 |
PRIDEi | Q12328 |
Genome annotation databases
EnsemblFungii | YDL217C_mRNA; YDL217C; YDL217C |
GeneIDi | 851309 |
KEGGi | sce:YDL217C |
Organism-specific databases
SGDi | S000002376, TIM22 |
VEuPathDBi | FungiDB:YDL217C |
Phylogenomic databases
eggNOGi | KOG3225, Eukaryota |
GeneTreei | ENSGT00390000016067 |
HOGENOMi | CLU_091077_1_0_1 |
InParanoidi | Q12328 |
OMAi | THSYAKN |
Enzyme and pathway databases
Reactomei | R-SCE-1268020, Mitochondrial protein import |
Miscellaneous databases
PROi | PR:Q12328 |
RNActi | Q12328, protein |
Family and domain databases
InterProi | View protein in InterPro IPR039175, TIM22 |
PANTHERi | PTHR14110, PTHR14110, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TIM22_YEAST | |
Accessioni | Q12328Primary (citable) accession number: Q12328 Secondary accession number(s): D6VRD7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families