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UniProtKB - Q12317 (MSB4_YEAST)
Protein
GTPase-activating protein MSB4
Gene
MSB4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Regulates exocytosis by functioning as a GAP for SEC4. Also required for efficient polarization of the actin patches.
2 PublicationsMiscellaneous
Present with 1730 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- GTPase activator activity Source: SGD
GO - Biological processi
- activation of GTPase activity Source: GO_Central
- exocytosis Source: SGD
- formin-nucleated actin cable assembly Source: SGD
- intracellular protein transport Source: GO_Central
Keywordsi
Molecular function | GTPase activation |
Names & Taxonomyi
Protein namesi | Recommended name: GTPase-activating protein MSB4Alternative name(s): Multicopy suppressor of bud emergence 4 |
Gene namesi | Name:MSB4 Ordered Locus Names:YOL112W ORF Names:HRC492 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005472, MSB4 |
VEuPathDBi | FungiDB:YOL112W |
Subcellular locationi
Other locations
- cellular bud neck Source: UniProtKB-SubCell
- cellular bud tip Source: SGD
- incipient cellular bud site Source: SGD
- polarisome Source: SGD
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 200 | R → F: Reduced GAP activity. 1 Publication | 1 | |
Mutagenesisi | 200 | R → K: Reduced GAP activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000208020 | 1 – 492 | GTPase-activating protein MSB4Add BLAST | 492 |
Proteomic databases
MaxQBi | Q12317 |
PaxDbi | Q12317 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 34288, 217 interactors |
ComplexPortali | CPX-3188, Polarisome |
DIPi | DIP-2784N |
IntActi | Q12317, 1 interactor |
MINTi | Q12317 |
STRINGi | 4932.YOL112W |
Miscellaneous databases
RNActi | Q12317, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 147 – 367 | Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST | 221 |
Phylogenomic databases
eggNOGi | KOG2058, Eukaryota |
GeneTreei | ENSGT00940000176552 |
HOGENOMi | CLU_005350_12_1_1 |
InParanoidi | Q12317 |
OMAi | FKQMTCG |
Family and domain databases
InterProi | View protein in InterPro IPR000195, Rab-GTPase-TBC_dom IPR035969, Rab-GTPase_TBC_sf |
Pfami | View protein in Pfam PF00566, RabGAP-TBC, 1 hit |
SMARTi | View protein in SMART SM00164, TBC, 1 hit |
SUPFAMi | SSF47923, SSF47923, 2 hits |
PROSITEi | View protein in PROSITE PS50086, TBC_RABGAP, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12317-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIMSSTMSTE AALVPNESVF DTVSSFNEDD ANYSVLDLYD DDDEGDDSST
60 70 80 90 100
VERKEILTTR ELEKAKAFTS LIMADPENFD RYGFSKKGYF ISQEEYDKWW
110 120 130 140 150
TEYNRYTERR KKKWENFLLK NKIELHNDNP LVYPARTDEL SKFVRKGIPA
160 170 180 190 200
EWRGNAWWYF AGGQRQLDAN VGVYDRLKSD CREGAVSGKD MEAIERDLYR
210 220 230 240 250
TFPDNIHFHK ESFQNGEPAI IRSLRRVLMA FSVYDKTIGY CQSMNFLVGL
260 270 280 290 300
LLLFMEEEKA FWMLVIITGK YLPGVYESDL EGANVDQGVL VLCIKEYLPE
310 320 330 340 350
IWSHIESSYM NGNGSTDQIS GPASGEEYLC RLPTLTLCTA SWFMSCFVGV
360 370 380 390 400
VPIETTLRIW DCLFYEESHF LFKVALGILK LSESEFLESK SQKLFRQYSS
410 420 430 440 450
YTFGGSNDSD STFKRLKNKI KTQEEADMEI LQVIQNFPKR LLNPNDIFEK
460 470 480 490
VLMKKKVALN GITQEKIDRG REYVAMARNR QRASSRPKER RK
Sequence cautioni
The sequence CAA88149 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA88150 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z48149 Genomic DNA Translation: CAA88149.1 Frameshift. Z48149 Genomic DNA Translation: CAA88150.1 Frameshift. Z48149 Genomic DNA Translation: CAA88148.1 Z74854 Genomic DNA Translation: CAA99131.1 BK006948 Genomic DNA Translation: DAA10671.1 |
PIRi | S51885 |
RefSeqi | NP_014529.1, NM_001183366.1 |
Genome annotation databases
EnsemblFungii | YOL112W_mRNA; YOL112W; YOL112W |
GeneIDi | 854037 |
KEGGi | sce:YOL112W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z48149 Genomic DNA Translation: CAA88149.1 Frameshift. Z48149 Genomic DNA Translation: CAA88150.1 Frameshift. Z48149 Genomic DNA Translation: CAA88148.1 Z74854 Genomic DNA Translation: CAA99131.1 BK006948 Genomic DNA Translation: DAA10671.1 |
PIRi | S51885 |
RefSeqi | NP_014529.1, NM_001183366.1 |
3D structure databases
SMRi | Q12317 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34288, 217 interactors |
ComplexPortali | CPX-3188, Polarisome |
DIPi | DIP-2784N |
IntActi | Q12317, 1 interactor |
MINTi | Q12317 |
STRINGi | 4932.YOL112W |
Proteomic databases
MaxQBi | Q12317 |
PaxDbi | Q12317 |
Genome annotation databases
EnsemblFungii | YOL112W_mRNA; YOL112W; YOL112W |
GeneIDi | 854037 |
KEGGi | sce:YOL112W |
Organism-specific databases
SGDi | S000005472, MSB4 |
VEuPathDBi | FungiDB:YOL112W |
Phylogenomic databases
eggNOGi | KOG2058, Eukaryota |
GeneTreei | ENSGT00940000176552 |
HOGENOMi | CLU_005350_12_1_1 |
InParanoidi | Q12317 |
OMAi | FKQMTCG |
Miscellaneous databases
PROi | PR:Q12317 |
RNActi | Q12317, protein |
Family and domain databases
InterProi | View protein in InterPro IPR000195, Rab-GTPase-TBC_dom IPR035969, Rab-GTPase_TBC_sf |
Pfami | View protein in Pfam PF00566, RabGAP-TBC, 1 hit |
SMARTi | View protein in SMART SM00164, TBC, 1 hit |
SUPFAMi | SSF47923, SSF47923, 2 hits |
PROSITEi | View protein in PROSITE PS50086, TBC_RABGAP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MSB4_YEAST | |
Accessioni | Q12317Primary (citable) accession number: Q12317 Secondary accession number(s): D6W1V5, Q05377, Q05378 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2005 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names