UniProtKB - Q12315 (GLE1_YEAST)

BLAST

>sp|Q12315|GLE1_YEAST Nucleoporin GLE1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GLE1 PE=1 SV=1
MRFVFDEVFNSDTDSPEFEETCSTTSSTSSQCPTPEPSPAIKLPSFTKVGTKKLVNESVV
ILDPALENALRDLNLQSKLIPINEPIVAASSIIVPHSTNMPLPRASHSSLLDNAKNSNAT
APLLEAIEESFQRKMQNLVLANQKEIQSIRENKRRVEEQRKRKEEEERKRKEAEEKAKRE
QELLRQKKDEEERKRKEAEAKLAQQKQEEERKKIEEQNEKERQLKKEHEAKLLQQKDKLG
KAVTNFDKISKMFWHYKDKIAQIKQDIVLPIKKADVNVRNLLSRHKRKINPKFGQLTNSN
QQLFKIQNELTQLINDTKGDSLAYHWILNFIAKAVVHQAETEVRVKPESALPLGKLTLYL
LVQFPELQELFMARLVKKCPFVIGFTCEIDTEKGRQNMGWKRNNENKWEDNTSYDERMGG
ILSLFAIITRLQLPQEFITTTSHPFPIALSWHILARICNTPLNLITNTHFVILGSWWDAA
AVQFLQAYGNQASKLLILIGEELTSRMAEKKYVGAARLRILLEAWQNNNMESFPEMSP

Align
Format
Add to basket Added to basket
History
Entry version 155 (12 Sep 2018)
Sequence version 1 (01 Nov 1997)
Previous versions | rss
Other tutorials and videos
Help video
Feedback

Protein

Nucleoporin GLE1

Gene

GLE1

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved in a terminal step of poly(A)+ mRNA transport through the NPC probably by binding the ATP-dependent RNA helicase DBP5 and GFD1 at the cytoplasmic side of the NPC. These interactions are thought to be important for the dissociation of transport proteins such as the heterogeneous nuclear ribonucleoprotein (hnRNP) NAB2 from exported mRNA.5 Publications

Miscellaneous

Present with 1040 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functionⁱ

enzyme activator activity
Source: SGD
Inferred from direct assayⁱ
- 16783364
inositol hexakisphosphate binding
Source: SGD
Inferred from direct assayⁱ
- 16783363
phospholipid binding
Source: SGD
Inferred from direct assayⁱ
- 17897934
translation initiation factor binding
Source: SGD
Inferred from physical interactionⁱ
- 18724935

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

mRNA export from nucleus
Source: SGD
Inferred from mutant phenotypeⁱ
- 20371601
mRNA processing Source: UniProtKB-KW
poly(A)+ mRNA export from nucleus
Source: SGD
Inferred from mutant phenotypeⁱ
- 27385342
- 8898365
regulation of translational initiation
Source: SGD
Inferred from mutant phenotypeⁱ
- 18724935
regulation of translational termination
Source: SGD
Inferred from mutant phenotypeⁱ
- 20371601

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	mRNA processing, mRNA transport, Protein transport, Translocation, Transport

Biological process

mRNA processing, mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29589-MONOMER

BioCycⁱ

YEAST:G3O-29589-MONOMER

Protein family/group databases

TCDBⁱ	1.I.1.1.1 the eukaryotic nuclear pore complex (e-npc) family

TCDBⁱ

1.I.1.1.1 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Nucleoporin GLE1 Alternative name(s): Nuclear pore protein GLE1 RNA export factor GLE1
Gene namesⁱ	Name:GLE1 Synonyms:BRR3, RSS1 Ordered Locus Names:YDL207W ORF Names:D1049
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome IV

Organism-specific databases

EuPathDBⁱ	FungiDB:YDL207W
Saccharomyces Genome Database More...SGDⁱ	S000002366 GLE1

Keywords - Cellular componentⁱ

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	351	L → A: Partial loss of activity. 1 Publication	1
Mutagenesisⁱ	353	L → A: Partial loss of activity. 1 Publication	1
Mutagenesisⁱ	356	L → A: Temperature-sensitive. 1 Publication	1
Mutagenesisⁱ	358	L → A: Partial loss of activity. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000204820	1 – 538	Nucleoporin GLE1Add BLAST		538

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q12315
PaxDbⁱ	Q12315
PRIDEⁱ	Q12315

PTM databases

iPTMnetⁱ	Q12315

iPTMnetⁱ

Q12315

Interactionⁱ

Subunit structureⁱ

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEC13 and SEH1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. GLE1 interacts with the NUP82 subcomplex (NSP1, NUP82, NUP159) via NUP42. It also interacts with GFD1 and the ATP-dependent RNA helicase DBP5.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
DBP5	P20449	8	EBI-7635,EBI-5617
NUP42	P49686	7	EBI-7635,EBI-12310
SUP35	P05453	2	EBI-7635,EBI-6540

GO - Molecular functionⁱ

translation initiation factor binding
Source: SGD
Inferred from physical interactionⁱ
- 18724935

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	31839, 455 interactors
ComplexPortalⁱ	CPX-824 Nuclear pore complex
Database of interacting proteins More...DIPⁱ	DIP-2350N
IntActⁱ	Q12315, 36 interactors
Molecular INTeraction database More...MINTⁱ	Q12315
STRINGⁱ	4932.YDL207W

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	245 – 266	Combined sources	22
Helixⁱ	268 – 273	Combined sources	6
Helixⁱ	276 – 289	Combined sources	14
Helixⁱ	292 – 295	Combined sources	4
Helixⁱ	300 – 315	Combined sources	16
Turnⁱ	316 – 319	Combined sources	4
Helixⁱ	321 – 345	Combined sources	25
Helixⁱ	347 – 349	Combined sources	3
Helixⁱ	350 – 363	Combined sources	14
Helixⁱ	367 – 378	Combined sources	12
Helixⁱ	380 – 383	Combined sources	4
Helixⁱ	392 – 397	Combined sources	6
Helixⁱ	411 – 430	Combined sources	20
Turnⁱ	435 – 440	Combined sources	6
Helixⁱ	448 – 458	Combined sources	11
Helixⁱ	462 – 464	Combined sources	3
Helixⁱ	467 – 488	Combined sources	22
Helixⁱ	490 – 500	Combined sources	11
Helixⁱ	502 – 506	Combined sources	5
Helixⁱ	508 – 510	Combined sources	3
Helixⁱ	513 – 526	Combined sources	14

Show more details

3D structure databases

ProteinModelPortalⁱ	Q12315
SMRⁱ	Q12315
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

Q12315

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	257 – 538	Interactions with NUP42, DBP5 and GFD1Add BLAST		282

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	131 – 244	Sequence analysisAdd BLAST		114

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	154 – 170	Bipartite nuclear localization signal 1Add BLAST		17
Motifⁱ	272 – 288	Bipartite nuclear localization signal 2Add BLAST		17

Compositional bias

Feature key	Position(s)	DescriptionActions	Length
Compositional biasⁱ	157 – 229	Glu-richAdd BLAST	73
Compositional biasⁱ	161 – 273	Lys-richAdd BLAST	113
Compositional biasⁱ	164 – 167	Poly-Glu	4

Sequence similaritiesⁱ

Belongs to the GLE1 family.Curated

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000012903
HOGENOMⁱ	HOG000248676
InParanoidⁱ	Q12315
KEGG Orthology (KO) More...KOⁱ	K18723
OMAⁱ	YVELHQH
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C341X

Family and domain databases

Gene3Dⁱ	1.25.40.510, 1 hit
InterProⁱ	View protein in InterPro IPR012476 GLE1 IPR038506 GLE1-like_sf
PANTHERⁱ	PTHR12960 PTHR12960, 2 hits
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07817 GLE1, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q12315-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRFVFDEVFN SDTDSPEFEE TCSTTSSTSS QCPTPEPSPA IKLPSFTKVG 
        60         70         80         90        100
TKKLVNESVV ILDPALENAL RDLNLQSKLI PINEPIVAAS SIIVPHSTNM 
       110        120        130        140        150
PLPRASHSSL LDNAKNSNAT APLLEAIEES FQRKMQNLVL ANQKEIQSIR 
       160        170        180        190        200
ENKRRVEEQR KRKEEEERKR KEAEEKAKRE QELLRQKKDE EERKRKEAEA 
       210        220        230        240        250
KLAQQKQEEE RKKIEEQNEK ERQLKKEHEA KLLQQKDKLG KAVTNFDKIS 
       260        270        280        290        300
KMFWHYKDKI AQIKQDIVLP IKKADVNVRN LLSRHKRKIN PKFGQLTNSN 
       310        320        330        340        350
QQLFKIQNEL TQLINDTKGD SLAYHWILNF IAKAVVHQAE TEVRVKPESA 
       360        370        380        390        400
LPLGKLTLYL LVQFPELQEL FMARLVKKCP FVIGFTCEID TEKGRQNMGW 
       410        420        430        440        450
KRNNENKWED NTSYDERMGG ILSLFAIITR LQLPQEFITT TSHPFPIALS 
       460        470        480        490        500
WHILARICNT PLNLITNTHF VILGSWWDAA AVQFLQAYGN QASKLLILIG 
       510        520        530 
EELTSRMAEK KYVGAARLRI LLEAWQNNNM ESFPEMSP

Length:538

Mass (Da):62,073

Last modified:November 1, 1997 - v1

Checksum:ⁱC9C6B1513AF95711

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U68475 Genomic DNA Translation: AAC49444.1 X99000 Genomic DNA Translation: CAA67484.1 Z74255 Genomic DNA Translation: CAA98785.1 BK006938 Genomic DNA Translation: DAA11657.1
PIRⁱ	S67766
NCBI Reference Sequences More...RefSeqⁱ	NP_010074.1, NM_001180267.1

Genome annotation databases

EnsemblFungiⁱ	YDL207W; YDL207W; YDL207W
GeneIDⁱ	851320
KEGGⁱ	sce:YDL207W

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q12315	Gle1p		YEASC		538	UniRef100_Q12315

Protein	Similar proteins		Species	Score	Length	Source
Q12315	Gle1p		YEASC		538	UniRef90_Q12315
Gle1p		YEASB		538
Nucleoporin GLE1		YEAS1		538
GLE1p Cytoplasmic nucleoporin required for polyadenylated mRNA export		Saccharomyces sp. 'boulardii'		538
Nuclear pore complex subunit		YEAS7		538
+6

Protein	Similar proteins		Species	Score	Length	Source
Q12315	Gle1p		YEASC		538	UniRef50_Q12315
K7_Gle1p		YEASK		538
Gle1p		YEASV		538
YDL207Wp-like protein		YEAS6		538
Gle1p		YEASB		538
+12

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U68475 Genomic DNA Translation: AAC49444.1 X99000 Genomic DNA Translation: CAA67484.1 Z74255 Genomic DNA Translation: CAA98785.1 BK006938 Genomic DNA Translation: DAA11657.1
PIRⁱ	S67766
RefSeqⁱ	NP_010074.1, NM_001180267.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	3PEU	X-ray	2.60	B	244-538	[»]
	3PEV	X-ray	2.50	B	244-538	[»]
	3RRM	X-ray	2.88	B	244-538	[»]
	3RRN	X-ray	4.00	B	244-538	[»]
	6B4E	X-ray	1.75	A/B	244-538	[»]
ProteinModelPortalⁱ	Q12315
SMRⁱ	Q12315
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	31839, 455 interactors
ComplexPortalⁱ	CPX-824 Nuclear pore complex
DIPⁱ	DIP-2350N
IntActⁱ	Q12315, 36 interactors
MINTⁱ	Q12315
STRINGⁱ	4932.YDL207W

Protein family/group databases

TCDBⁱ	1.I.1.1.1 the eukaryotic nuclear pore complex (e-npc) family

TCDBⁱ

1.I.1.1.1 the eukaryotic nuclear pore complex (e-npc) family

PTM databases

iPTMnetⁱ	Q12315

iPTMnetⁱ

Q12315

Proteomic databases

MaxQBⁱ	Q12315
PaxDbⁱ	Q12315
PRIDEⁱ	Q12315

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YDL207W; YDL207W; YDL207W
GeneIDⁱ	851320
KEGGⁱ	sce:YDL207W

Organism-specific databases

EuPathDBⁱ	FungiDB:YDL207W
SGDⁱ	S000002366 GLE1

Phylogenomic databases

GeneTreeⁱ	ENSGT00390000012903
HOGENOMⁱ	HOG000248676
InParanoidⁱ	Q12315
KOⁱ	K18723
OMAⁱ	YVELHQH
OrthoDBⁱ	EOG092C341X

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29589-MONOMER

BioCycⁱ

YEAST:G3O-29589-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	Q12315
Protein Ontology More...PROⁱ	PR:Q12315

Family and domain databases

Gene3Dⁱ	1.25.40.510, 1 hit
InterProⁱ	View protein in InterPro IPR012476 GLE1 IPR038506 GLE1-like_sf
PANTHERⁱ	PTHR12960 PTHR12960, 2 hits
Pfamⁱ	View protein in Pfam PF07817 GLE1, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GLE1_YEAST
Accessionⁱ	Q12315Primary (citable) accession number: Q12315 Secondary accession number(s): D6VRE7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	November 1, 1997
	Last modified:	September 12, 2018
	This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q12315 (GLE1_YEAST)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Nucleoporin GLE1

GLE1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Coiled coil

Motif

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ