Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nucleoporin GLE1

Gene

GLE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved in a terminal step of poly(A)+ mRNA transport through the NPC probably by binding the ATP-dependent RNA helicase DBP5 and GFD1 at the cytoplasmic side of the NPC. These interactions are thought to be important for the dissociation of transport proteins such as the heterogeneous nuclear ribonucleoprotein (hnRNP) NAB2 from exported mRNA.5 Publications

Miscellaneous

Present with 1040 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • enzyme activator activity Source: SGD
  • inositol hexakisphosphate binding Source: SGD
  • phospholipid binding Source: SGD
  • translation initiation factor binding Source: SGD

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • mRNA processing Source: UniProtKB-KW
  • poly(A)+ mRNA export from nucleus Source: SGD
  • regulation of translational initiation Source: SGD
  • regulation of translational termination Source: SGD

Keywordsi

Biological processmRNA processing, mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29589-MONOMER

Protein family/group databases

TCDBi1.I.1.1.1 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin GLE1
Alternative name(s):
Nuclear pore protein GLE1
RNA export factor GLE1
Gene namesi
Name:GLE1
Synonyms:BRR3, RSS1
Ordered Locus Names:YDL207W
ORF Names:D1049
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL207W
SGDiS000002366 GLE1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi351L → A: Partial loss of activity. 1 Publication1
Mutagenesisi353L → A: Partial loss of activity. 1 Publication1
Mutagenesisi356L → A: Temperature-sensitive. 1 Publication1
Mutagenesisi358L → A: Partial loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048201 – 538Nucleoporin GLE1Add BLAST538

Proteomic databases

MaxQBiQ12315
PaxDbiQ12315
PRIDEiQ12315

PTM databases

iPTMnetiQ12315

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEC13 and SEH1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. GLE1 interacts with the NUP82 subcomplex (NSP1, NUP82, NUP159) via NUP42. It also interacts with GFD1 and the ATP-dependent RNA helicase DBP5.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • translation initiation factor binding Source: SGD

Protein-protein interaction databases

BioGridi31839, 455 interactors
ComplexPortaliCPX-824 Nuclear pore complex
DIPiDIP-2350N
IntActiQ12315, 36 interactors
MINTiQ12315
STRINGi4932.YDL207W

Structurei

Secondary structure

1538
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi245 – 266Combined sources22
Helixi268 – 273Combined sources6
Helixi276 – 289Combined sources14
Helixi292 – 295Combined sources4
Helixi300 – 315Combined sources16
Turni316 – 319Combined sources4
Helixi321 – 345Combined sources25
Helixi347 – 349Combined sources3
Helixi350 – 363Combined sources14
Helixi367 – 378Combined sources12
Helixi380 – 383Combined sources4
Helixi392 – 397Combined sources6
Helixi411 – 430Combined sources20
Turni435 – 440Combined sources6
Helixi448 – 458Combined sources11
Helixi462 – 464Combined sources3
Helixi467 – 488Combined sources22
Helixi490 – 500Combined sources11
Helixi502 – 506Combined sources5
Helixi508 – 510Combined sources3
Helixi513 – 526Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PEUX-ray2.60B244-538[»]
3PEVX-ray2.50B244-538[»]
3RRMX-ray2.88B244-538[»]
3RRNX-ray4.00B244-538[»]
ProteinModelPortaliQ12315
SMRiQ12315
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12315

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni257 – 538Interactions with NUP42, DBP5 and GFD1Add BLAST282

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili131 – 244Sequence analysisAdd BLAST114

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi154 – 170Bipartite nuclear localization signal 1Add BLAST17
Motifi272 – 288Bipartite nuclear localization signal 2Add BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi157 – 229Glu-richAdd BLAST73
Compositional biasi161 – 273Lys-richAdd BLAST113
Compositional biasi164 – 167Poly-Glu4

Sequence similaritiesi

Belongs to the GLE1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000012903
HOGENOMiHOG000248676
InParanoidiQ12315
KOiK18723
OMAiYVELHQH
OrthoDBiEOG092C341X

Family and domain databases

Gene3Di1.25.40.510, 1 hit
InterProiView protein in InterPro
IPR012476 GLE1
IPR038506 GLE1-like_sf
PANTHERiPTHR12960 PTHR12960, 1 hit
PfamiView protein in Pfam
PF07817 GLE1, 1 hit

Sequencei

Sequence statusi: Complete.

Q12315-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFVFDEVFN SDTDSPEFEE TCSTTSSTSS QCPTPEPSPA IKLPSFTKVG
60 70 80 90 100
TKKLVNESVV ILDPALENAL RDLNLQSKLI PINEPIVAAS SIIVPHSTNM
110 120 130 140 150
PLPRASHSSL LDNAKNSNAT APLLEAIEES FQRKMQNLVL ANQKEIQSIR
160 170 180 190 200
ENKRRVEEQR KRKEEEERKR KEAEEKAKRE QELLRQKKDE EERKRKEAEA
210 220 230 240 250
KLAQQKQEEE RKKIEEQNEK ERQLKKEHEA KLLQQKDKLG KAVTNFDKIS
260 270 280 290 300
KMFWHYKDKI AQIKQDIVLP IKKADVNVRN LLSRHKRKIN PKFGQLTNSN
310 320 330 340 350
QQLFKIQNEL TQLINDTKGD SLAYHWILNF IAKAVVHQAE TEVRVKPESA
360 370 380 390 400
LPLGKLTLYL LVQFPELQEL FMARLVKKCP FVIGFTCEID TEKGRQNMGW
410 420 430 440 450
KRNNENKWED NTSYDERMGG ILSLFAIITR LQLPQEFITT TSHPFPIALS
460 470 480 490 500
WHILARICNT PLNLITNTHF VILGSWWDAA AVQFLQAYGN QASKLLILIG
510 520 530
EELTSRMAEK KYVGAARLRI LLEAWQNNNM ESFPEMSP
Length:538
Mass (Da):62,073
Last modified:November 1, 1997 - v1
Checksum:iC9C6B1513AF95711
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68475 Genomic DNA Translation: AAC49444.1
X99000 Genomic DNA Translation: CAA67484.1
Z74255 Genomic DNA Translation: CAA98785.1
BK006938 Genomic DNA Translation: DAA11657.1
PIRiS67766
RefSeqiNP_010074.1, NM_001180267.1

Genome annotation databases

EnsemblFungiiYDL207W; YDL207W; YDL207W
GeneIDi851320
KEGGisce:YDL207W

Similar proteinsi

Entry informationi

Entry nameiGLE1_YEAST
AccessioniPrimary (citable) accession number: Q12315
Secondary accession number(s): D6VRE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 20, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health