UniProtKB - Q12315 (GLE1_YEAST)
Nucleoporin GLE1
GLE1
Functioni
Miscellaneous
GO - Molecular functioni
- enzyme activator activity Source: SGD
- inositol hexakisphosphate binding Source: SGD
- phospholipid binding Source: SGD
- translation initiation factor binding Source: SGD
GO - Biological processi
- mRNA export from nucleus Source: SGD
- mRNA processing Source: UniProtKB-KW
- poly(A)+ mRNA export from nucleus Source: SGD
- regulation of translational initiation Source: SGD
- regulation of translational termination Source: SGD
- tRNA export from nucleus Source: SGD
Keywordsi
Biological process | mRNA processing, mRNA transport, Protein transport, Translocation, Transport |
Protein family/group databases
TCDBi | 1.I.1.1.1, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Nucleoporin GLE1Alternative name(s): Nuclear pore protein GLE1 RNA export factor GLE1 |
Gene namesi | Name:GLE1 Synonyms:BRR3, RSS1 Ordered Locus Names:YDL207W ORF Names:D1049 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002366, GLE1 |
VEuPathDBi | FungiDB:YDL207W |
Subcellular locationi
Nucleus
- nuclear pore complex 1 Publication
- Nucleus membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
- Nucleus membrane 1 Publication; Peripheral membrane protein 1 Publication; Nucleoplasmic side 1 Publication
Note: Biased towards cytoplasmic side.
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nuclear membrane Source: UniProtKB-SubCell
- nuclear pore Source: SGD
- nuclear pore cytoplasmic filaments Source: SGD
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
Membrane, Nuclear pore complex, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 351 | L → A: Partial loss of activity. 1 Publication | 1 | |
Mutagenesisi | 353 | L → A: Partial loss of activity. 1 Publication | 1 | |
Mutagenesisi | 356 | L → A: Temperature-sensitive. 1 Publication | 1 | |
Mutagenesisi | 358 | L → A: Partial loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204820 | 1 – 538 | Nucleoporin GLE1Add BLAST | 538 |
Proteomic databases
MaxQBi | Q12315 |
PaxDbi | Q12315 |
PRIDEi | Q12315 |
PTM databases
iPTMneti | Q12315 |
Interactioni
Subunit structurei
Component of the nuclear pore complex (NPC). NPC constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. GLE1 interacts with the NUP82 subcomplex via NUP42. It also interacts with GFD1 and the ATP-dependent RNA helicase DBP5.
2 PublicationsBinary interactionsi
Hide detailsQ12315
With | #Exp. | IntAct |
---|---|---|
DBP5 [P20449] | 8 | EBI-7635,EBI-5617 |
NUP42 [P49686] | 7 | EBI-7635,EBI-12310 |
SUP35 [P05453] | 2 | EBI-7635,EBI-6540 |
GO - Molecular functioni
- translation initiation factor binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 31839, 455 interactors |
ComplexPortali | CPX-824, Nuclear pore complex |
DIPi | DIP-2350N |
IntActi | Q12315, 37 interactors |
MINTi | Q12315 |
STRINGi | 4932.YDL207W |
Miscellaneous databases
RNActi | Q12315, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q12315 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q12315 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 257 – 538 | Interactions with NUP42, DBP5 and GFD1Add BLAST | 282 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 131 – 244 | Sequence analysisAdd BLAST | 114 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 154 – 170 | Bipartite nuclear localization signal 1Add BLAST | 17 | |
Motifi | 272 – 288 | Bipartite nuclear localization signal 2Add BLAST | 17 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 157 – 229 | Glu-richAdd BLAST | 73 | |
Compositional biasi | 161 – 273 | Lys-richAdd BLAST | 113 | |
Compositional biasi | 164 – 167 | Poly-Glu | 4 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2412, Eukaryota |
GeneTreei | ENSGT00390000012903 |
HOGENOMi | CLU_029651_0_0_1 |
InParanoidi | Q12315 |
OMAi | HDPYEEW |
Family and domain databases
Gene3Di | 1.25.40.510, 1 hit |
InterProi | View protein in InterPro IPR012476, GLE1 IPR038506, GLE1-like_sf |
PANTHERi | PTHR12960, PTHR12960, 1 hit |
Pfami | View protein in Pfam PF07817, GLE1, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MRFVFDEVFN SDTDSPEFEE TCSTTSSTSS QCPTPEPSPA IKLPSFTKVG
60 70 80 90 100
TKKLVNESVV ILDPALENAL RDLNLQSKLI PINEPIVAAS SIIVPHSTNM
110 120 130 140 150
PLPRASHSSL LDNAKNSNAT APLLEAIEES FQRKMQNLVL ANQKEIQSIR
160 170 180 190 200
ENKRRVEEQR KRKEEEERKR KEAEEKAKRE QELLRQKKDE EERKRKEAEA
210 220 230 240 250
KLAQQKQEEE RKKIEEQNEK ERQLKKEHEA KLLQQKDKLG KAVTNFDKIS
260 270 280 290 300
KMFWHYKDKI AQIKQDIVLP IKKADVNVRN LLSRHKRKIN PKFGQLTNSN
310 320 330 340 350
QQLFKIQNEL TQLINDTKGD SLAYHWILNF IAKAVVHQAE TEVRVKPESA
360 370 380 390 400
LPLGKLTLYL LVQFPELQEL FMARLVKKCP FVIGFTCEID TEKGRQNMGW
410 420 430 440 450
KRNNENKWED NTSYDERMGG ILSLFAIITR LQLPQEFITT TSHPFPIALS
460 470 480 490 500
WHILARICNT PLNLITNTHF VILGSWWDAA AVQFLQAYGN QASKLLILIG
510 520 530
EELTSRMAEK KYVGAARLRI LLEAWQNNNM ESFPEMSP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U68475 Genomic DNA Translation: AAC49444.1 X99000 Genomic DNA Translation: CAA67484.1 Z74255 Genomic DNA Translation: CAA98785.1 BK006938 Genomic DNA Translation: DAA11657.1 |
PIRi | S67766 |
RefSeqi | NP_010074.1, NM_001180267.1 |
Genome annotation databases
EnsemblFungii | YDL207W_mRNA; YDL207W; YDL207W |
GeneIDi | 851320 |
KEGGi | sce:YDL207W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U68475 Genomic DNA Translation: AAC49444.1 X99000 Genomic DNA Translation: CAA67484.1 Z74255 Genomic DNA Translation: CAA98785.1 BK006938 Genomic DNA Translation: DAA11657.1 |
PIRi | S67766 |
RefSeqi | NP_010074.1, NM_001180267.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3PEU | X-ray | 2.60 | B | 244-538 | [»] | |
3PEV | X-ray | 2.50 | B | 244-538 | [»] | |
3RRM | X-ray | 2.88 | B | 244-538 | [»] | |
3RRN | X-ray | 4.00 | B | 244-538 | [»] | |
6B4E | X-ray | 1.75 | A/B | 244-538 | [»] | |
SMRi | Q12315 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31839, 455 interactors |
ComplexPortali | CPX-824, Nuclear pore complex |
DIPi | DIP-2350N |
IntActi | Q12315, 37 interactors |
MINTi | Q12315 |
STRINGi | 4932.YDL207W |
Protein family/group databases
TCDBi | 1.I.1.1.1, the nuclear pore complex (npc) family |
PTM databases
iPTMneti | Q12315 |
Proteomic databases
MaxQBi | Q12315 |
PaxDbi | Q12315 |
PRIDEi | Q12315 |
Genome annotation databases
EnsemblFungii | YDL207W_mRNA; YDL207W; YDL207W |
GeneIDi | 851320 |
KEGGi | sce:YDL207W |
Organism-specific databases
SGDi | S000002366, GLE1 |
VEuPathDBi | FungiDB:YDL207W |
Phylogenomic databases
eggNOGi | KOG2412, Eukaryota |
GeneTreei | ENSGT00390000012903 |
HOGENOMi | CLU_029651_0_0_1 |
InParanoidi | Q12315 |
OMAi | HDPYEEW |
Miscellaneous databases
EvolutionaryTracei | Q12315 |
PROi | PR:Q12315 |
RNActi | Q12315, protein |
Family and domain databases
Gene3Di | 1.25.40.510, 1 hit |
InterProi | View protein in InterPro IPR012476, GLE1 IPR038506, GLE1-like_sf |
PANTHERi | PTHR12960, PTHR12960, 1 hit |
Pfami | View protein in Pfam PF07817, GLE1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLE1_YEAST | |
Accessioni | Q12315Primary (citable) accession number: Q12315 Secondary accession number(s): D6VRE7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names