UniProtKB - Q12285 (MDY2_YEAST)
Protein
Ubiquitin-like protein MDY2
Gene
MDY2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.2 Publications
Miscellaneous
Present with 6510 molecules/cell in log phase SD medium.1 Publication
GO - Biological processi
- cell morphogenesis involved in conjugation with cellular fusion Source: SGD
- posttranslational protein targeting to endoplasmic reticulum membrane Source: SGD
- protein insertion into ER membrane Source: SGD
Protein family/group databases
TCDBi | 3.A.21.1.1, the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-like protein MDY2Alternative name(s): Golgi to ER traffic protein 5 Mating-deficient protein 2 Translation machinery-associated protein 24 |
Gene namesi | Name:MDY2 Synonyms:GET5, TMA24 Ordered Locus Names:YOL111C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YOL111C |
SGDi | S000005471, MDY2 |
Subcellular locationi
Cytosol
- TRC complex Source: SGD
Nucleus
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
- cytoplasmic stress granule Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000235917 | 1 – 212 | Ubiquitin-like protein MDY2Add BLAST | 212 |
Proteomic databases
MaxQBi | Q12285 |
PaxDbi | Q12285 |
PRIDEi | Q12285 |
Interactioni
Subunit structurei
Interacts with GET4.
1 PublicationBinary interactionsi
Hide detailsQ12285
Protein-protein interaction databases
BioGRIDi | 34289, 331 interactors |
ComplexPortali | CPX-1861, GET4-GET5 transmembrane domain recognition complex |
DIPi | DIP-1982N |
IntActi | Q12285, 13 interactors |
MINTi | Q12285 |
STRINGi | 4932.YOL111C |
Miscellaneous databases
RNActi | Q12285, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q12285 |
SMRi | Q12285 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q12285 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 74 – 152 | Ubiquitin-likePROSITE-ProRule annotationAdd BLAST | 79 |
Phylogenomic databases
eggNOGi | KOG0011, Eukaryota |
HOGENOMi | CLU_1294717_0_0_1 |
InParanoidi | Q12285 |
OMAi | PEHEFVS |
Family and domain databases
InterProi | View protein in InterPro IPR040474, Get5_C IPR031765, Mdy2_get4-bd IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF16843, Get5_bdg, 1 hit PF18514, Get5_C, 1 hit PF00240, ubiquitin, 1 hit |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50053, UBIQUITIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12285-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTSASGPEH EFVSKFLTLA TLTEPKLPKS YTKPLKDVTN LGVPLPTLKY
60 70 80 90 100
KYKQNRAKKL KLHQDQQGQD NAAVHLTLKK IQAPKFSIEH DFSPSDTILQ
110 120 130 140 150
IKQHLISEEK ASHISEIKLL LKGKVLHDNL FLSDLKVTPA NSTITVMIKP
160 170 180 190 200
NPTISKEPEA EKSTNSPAPA PPQELTVPWD DIEALLKNNF ENDQAAVRQV
210
MERLQKGWSL AK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z48149 Genomic DNA Translation: CAA88151.1 Z74853 Genomic DNA Translation: CAA99130.1 BK006948 Genomic DNA Translation: DAA10672.1 |
PIRi | S51888 |
RefSeqi | NP_014530.1, NM_001183365.1 |
Genome annotation databases
EnsemblFungii | YOL111C_mRNA; YOL111C; YOL111C |
GeneIDi | 854038 |
KEGGi | sce:YOL111C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z48149 Genomic DNA Translation: CAA88151.1 Z74853 Genomic DNA Translation: CAA99130.1 BK006948 Genomic DNA Translation: DAA10672.1 |
PIRi | S51888 |
RefSeqi | NP_014530.1, NM_001183365.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LNZ | NMR | - | A/B | 152-212 | [»] | |
2LXA | NMR | - | A | 74-151 | [»] | |
2LXC | NMR | - | A | 74-151 | [»] | |
2WPV | X-ray | 1.99 | B/D/F/H | 1-59 | [»] | |
3LKU | X-ray | 2.80 | B/D/F | 3-56 | [»] | |
3VEJ | X-ray | 1.23 | A/B | 175-212 | [»] | |
3ZDM | X-ray | 1.80 | C/F | 71-151 | [»] | |
4A20 | X-ray | 1.78 | A | 70-152 | [»] | |
4ASW | NMR | - | C | 70-152 | [»] | |
4GOC | X-ray | 2.40 | A/B/C | 74-148 | [»] | |
4PWX | X-ray | 5.40 | D/F | 1-54 | [»] | |
5BW8 | X-ray | 2.80 | D | 1-54 | [»] | |
5BWK | X-ray | 6.00 | F/H/J/L/R/T/V/X | 1-56 | [»] | |
BMRBi | Q12285 | |||||
SMRi | Q12285 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34289, 331 interactors |
ComplexPortali | CPX-1861, GET4-GET5 transmembrane domain recognition complex |
DIPi | DIP-1982N |
IntActi | Q12285, 13 interactors |
MINTi | Q12285 |
STRINGi | 4932.YOL111C |
Protein family/group databases
TCDBi | 3.A.21.1.1, the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family |
Proteomic databases
MaxQBi | Q12285 |
PaxDbi | Q12285 |
PRIDEi | Q12285 |
Genome annotation databases
EnsemblFungii | YOL111C_mRNA; YOL111C; YOL111C |
GeneIDi | 854038 |
KEGGi | sce:YOL111C |
Organism-specific databases
EuPathDBi | FungiDB:YOL111C |
SGDi | S000005471, MDY2 |
Phylogenomic databases
eggNOGi | KOG0011, Eukaryota |
HOGENOMi | CLU_1294717_0_0_1 |
InParanoidi | Q12285 |
OMAi | PEHEFVS |
Miscellaneous databases
ChiTaRSi | MDY2, yeast |
EvolutionaryTracei | Q12285 |
PROi | PR:Q12285 |
RNActi | Q12285, protein |
Family and domain databases
InterProi | View protein in InterPro IPR040474, Get5_C IPR031765, Mdy2_get4-bd IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF16843, Get5_bdg, 1 hit PF18514, Get5_C, 1 hit PF00240, ubiquitin, 1 hit |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50053, UBIQUITIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MDY2_YEAST | |
Accessioni | Q12285Primary (citable) accession number: Q12285 Secondary accession number(s): D6W1V6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD