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UniProtKB - Q12263 (GIN4_YEAST)
Protein
Serine/threonine-protein kinase GIN4
Gene
GIN4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Serine/threonine-protein kinase which regulates the localization and the function of the septins during mitosis. Phosphorylates SHS1.
1 PublicationMiscellaneous
Present with 736 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 48 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 156 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 25 – 33 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein kinase activity Source: SGD
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: GO_Central
- protein serine kinase activity Source: RHEA
GO - Biological processi
- budding cell bud growth Source: SGD
- intracellular signal transduction Source: GO_Central
- mitotic morphogenesis checkpoint signaling Source: SGD
- positive regulation of protein serine/threonine kinase activity Source: ComplexPortal
- protein autophosphorylation Source: SGD
- protein phosphorylation Source: SGD
- regulation of protein localization to cell division site Source: SGD
- septin ring assembly Source: SGD
- septum digestion after cytokinesis Source: ComplexPortal
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 984 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase GIN4 (EC:2.7.11.1)Alternative name(s): Growth inhibitory protein 4 |
Gene namesi | Name:GIN4 Ordered Locus Names:YDR507C ORF Names:D9719.13 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002915, GIN4 |
VEuPathDBi | FungiDB:YDR507C |
Subcellular locationi
Cytoskeleton
- Gin4 complex Source: ComplexPortal
Nucleus
- nucleus Source: GO_Central
Other locations
- cellular bud neck Source: SGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085964 | 1 – 1142 | Serine/threonine-protein kinase GIN4Add BLAST | 1142 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 406 | PhosphoserineCombined sources | 1 | |
Modified residuei | 465 | PhosphoserineCombined sources | 1 | |
Modified residuei | 471 | PhosphoserineCombined sources | 1 | |
Modified residuei | 617 | PhosphoserineCombined sources | 1 | |
Modified residuei | 689 | PhosphoserineCombined sources | 1 | |
Modified residuei | 719 | PhosphoserineCombined sources | 1 | |
Modified residuei | 805 | PhosphoserineCombined sources | 1 | |
Modified residuei | 807 | PhosphoserineCombined sources | 1 | |
Modified residuei | 883 | PhosphoserineCombined sources | 1 | |
Modified residuei | 884 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 930 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q12263 |
PaxDbi | Q12263 |
PRIDEi | Q12263 |
PTM databases
iPTMneti | Q12263 |
Interactioni
Subunit structurei
Component of the GIN4 complex composed of at least BNI5, CDC3, CDC10, CDC11, CDC12, GIN4, NAP1 and SHS1 which forms a ring at the bud neck.
1 PublicationBinary interactionsi
Q12263
With | #Exp. | IntAct |
---|---|---|
CDC11 [P32458] | 7 | EBI-7595,EBI-4178 |
MKK1 [P32490] | 2 | EBI-7595,EBI-10968 |
NAP1 [P25293] | 11 | EBI-7595,EBI-11850 |
SHS1 [Q07657] | 7 | EBI-7595,EBI-22083 |
Protein-protein interaction databases
BioGRIDi | 32559, 323 interactors |
ComplexPortali | CPX-1712, Gin4 serine/threonine kinase complex |
DIPi | DIP-2260N |
IntActi | Q12263, 21 interactors |
MINTi | Q12263 |
STRINGi | 4932.YDR507C |
Miscellaneous databases
RNActi | Q12263, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 19 – 289 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 271 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 378 – 412 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 425 – 469 | DisorderedSequence analysisAdd BLAST | 45 | |
Regioni | 573 – 592 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 676 – 698 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 903 – 1031 | DisorderedSequence analysisAdd BLAST | 129 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 950 – 994 | Basic and acidic residuesSequence analysisAdd BLAST | 45 | |
Compositional biasi | 1001 – 1022 | Basic and acidic residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0588, Eukaryota |
GeneTreei | ENSGT00940000166887 |
HOGENOMi | CLU_005276_0_0_1 |
InParanoidi | Q12263 |
OMAi | IFRKLSW |
Family and domain databases
Gene3Di | 3.30.310.220, 1 hit |
InterProi | View protein in InterPro IPR031850, Fungal_KA1_dom IPR043024, KA1_sf_fungal IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF16797, Fungal_KA1, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12263-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAINGNSIPA IKDNTIGPWK LGETLGLGST GKVQLARNGS TGQEAAVKVI
60 70 80 90 100
SKAVFNTGNV SGTSIVGSTT PDALPYGIER EIIIMKLLNH PNVLRLYDVW
110 120 130 140 150
ETNTDLYLVL EYAEKGELFN LLVERGPLPE HEAIRFFRQI IIGVSYCHAL
160 170 180 190 200
GIVHRDLKPE NLLLDHKYNI KIADFGMAAL ETEGKLLETS CGSPHYAAPE
210 220 230 240 250
IVSGIPYQGF ASDVWSCGVI LFALLTGRLP FDEEDGNIRT LLLKVQKGEF
260 270 280 290 300
EMPSDDEISR EAQDLIRKIL TVDPERRIKT RDILKHPLLQ KYPSIRDSKS
310 320 330 340 350
IRGLPREDTY LTPLSESNSS IDATILQNLV ILWHGRDPEG IKEKLREPGA
360 370 380 390 400
NAEKTLYALL YRFKCDTQKE LIKQQQVKKR QSISSVSVSP SKKVSTTPQR
410 420 430 440 450
RRNRESLISV TSSRKKPISF NKFTASSASS SNLTTPGSSK RLSKNFSSKK
460 470 480 490 500
KLSTIVNQSS PTPASRNKRA SVINVEKNQK RASIFSTTKK NKRSSRSIKR
510 520 530 540 550
MSLIPSMKRE SVTTKLMSTY AKLAEDDDWE YIEKETKRTS SNFATLIDEI
560 570 580 590 600
FEYEKYEQIR KEKEELERKV REAKAREELE RRRRKQEEKE RARKLLEKED
610 620 630 640 650
LKRKQEELKK QIEIDISDLE QELSKHKEEK LDGNIRSISA PMENEEKNIN
660 670 680 690 700
HLEVDIDNIL RRRNFSLQTR PVSRLDPGIM FSSPTEEVSP VEPKRTENER
710 720 730 740 750
LTTEKKILET IRRSKFLGSS FNIDKELKLS KMEYPSIIAP QRLSEERVVS
760 770 780 790 800
DSNDGYESLI LPKDGNGVSQ LKDSTATTAP VSDGRLRKIS EIRVPQFTRK
810 820 830 840 850
SRHFSESNKR LSVLSMYSTK ESFTNLVDIL KNGNLDVNNQ QSQRIPTPRS
860 870 880 890 900
ADDSEFLFET VNEEAEYTGN SSNDERLYDV GDSTIKDKSA LKLNFADRFN
910 920 930 940 950
GSNEAKQTDN LHLPILPPLN GDNELRKQNS QEGDQAHPKI KSMIPESGSS
960 970 980 990 1000
SHTEKEEENE EKEEKKPEQH KQEEDQEKRE KVVDDMEPPL NKSVQKIREK
1010 1020 1030 1040 1050
NAGSQAKDHS KDHLKEHKQD KNTAIGNGSF FRKFSKSSDK TMELYAKISA
1060 1070 1080 1090 1100
KQLFNGLEKL LRGWTQYGLK NIKSHPNNLT LTGKLSSDNI FSLRSTLFEV
1110 1120 1130 1140
NIYPRGKMSV VQFKKVSGSF KAVKKLVNEV ENVLNKEGVL QK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U33140 Genomic DNA Translation: AAA75513.1 U33057 Genomic DNA Translation: AAB64949.1 BK006938 Genomic DNA Translation: DAA12339.1 |
PIRi | S59359 |
RefSeqi | NP_010795.3, NM_001180815.3 |
Genome annotation databases
EnsemblFungii | YDR507C_mRNA; YDR507C; YDR507C |
GeneIDi | 852119 |
KEGGi | sce:YDR507C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U33140 Genomic DNA Translation: AAA75513.1 U33057 Genomic DNA Translation: AAB64949.1 BK006938 Genomic DNA Translation: DAA12339.1 |
PIRi | S59359 |
RefSeqi | NP_010795.3, NM_001180815.3 |
3D structure databases
AlphaFoldDBi | Q12263 |
SMRi | Q12263 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 32559, 323 interactors |
ComplexPortali | CPX-1712, Gin4 serine/threonine kinase complex |
DIPi | DIP-2260N |
IntActi | Q12263, 21 interactors |
MINTi | Q12263 |
STRINGi | 4932.YDR507C |
PTM databases
iPTMneti | Q12263 |
Proteomic databases
MaxQBi | Q12263 |
PaxDbi | Q12263 |
PRIDEi | Q12263 |
Genome annotation databases
EnsemblFungii | YDR507C_mRNA; YDR507C; YDR507C |
GeneIDi | 852119 |
KEGGi | sce:YDR507C |
Organism-specific databases
SGDi | S000002915, GIN4 |
VEuPathDBi | FungiDB:YDR507C |
Phylogenomic databases
eggNOGi | KOG0588, Eukaryota |
GeneTreei | ENSGT00940000166887 |
HOGENOMi | CLU_005276_0_0_1 |
InParanoidi | Q12263 |
OMAi | IFRKLSW |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 984 |
Miscellaneous databases
PROi | PR:Q12263 |
RNActi | Q12263, protein |
Family and domain databases
Gene3Di | 3.30.310.220, 1 hit |
InterProi | View protein in InterPro IPR031850, Fungal_KA1_dom IPR043024, KA1_sf_fungal IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF16797, Fungal_KA1, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GIN4_YEAST | |
Accessioni | Q12263Primary (citable) accession number: Q12263 Secondary accession number(s): D6VTC9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 197 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - SIMILARITY comments
Index of protein domains and families