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Protein

Polyamine transporter 4

Gene

TPO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Recognizes spermidine, spermine and the antimalarial drug quinidine, but not quinine, chloroquine and mefloquine.3 Publications

Miscellaneous

Present with 21400 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • drug transmembrane transporter activity Source: GO_Central
  • spermidine transmembrane transporter activity Source: SGD
  • spermine transmembrane transporter activity Source: SGD

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processAntiport, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-33763-MONOMER

Protein family/group databases

Transport Classification Database

More...
TCDBi
2.A.1.2.64 the major facilitator superfamily (mfs)

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Polyamine transporter 4
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:TPO4
Ordered Locus Names:YOR273C
ORF Names:O5440
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XV

Organism-specific databases

Saccharomyces Genome Database

More...
SGDi
S000005799 TPO4

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 99CytoplasmicSequence analysisAdd BLAST99
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Topological domaini121 – 128ExtracellularSequence analysis8
Transmembranei129 – 149HelicalSequence analysisAdd BLAST21
Topological domaini150 – 157CytoplasmicSequence analysis8
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Topological domaini179 – 187ExtracellularSequence analysis9
Transmembranei188 – 208HelicalSequence analysisAdd BLAST21
Topological domaini209 – 215CytoplasmicSequence analysis7
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Topological domaini237 – 246ExtracellularSequence analysis10
Transmembranei247 – 267HelicalSequence analysisAdd BLAST21
Topological domaini268 – 316CytoplasmicSequence analysisAdd BLAST49
Transmembranei317 – 337HelicalSequence analysisAdd BLAST21
Topological domaini338 – 355ExtracellularSequence analysisAdd BLAST18
Transmembranei356 – 376HelicalSequence analysisAdd BLAST21
Topological domaini377 – 423CytoplasmicSequence analysisAdd BLAST47
Transmembranei424 – 444HelicalSequence analysisAdd BLAST21
Topological domaini445 – 456ExtracellularSequence analysisAdd BLAST12
Transmembranei457 – 477HelicalSequence analysisAdd BLAST21
Topological domaini478 – 486CytoplasmicSequence analysis9
Transmembranei487 – 509HelicalSequence analysisAdd BLAST23
Topological domaini510 – 518ExtracellularSequence analysis9
Transmembranei519 – 539HelicalSequence analysisAdd BLAST21
Topological domaini540 – 659CytoplasmicSequence analysisAdd BLAST120

Keywords - Cellular componenti

Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002627321 – 659Polyamine transporter 4Add BLAST659

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei589PhosphothreonineCombined sources1
Modified residuei606PhosphothreonineCombined sources1
Modified residuei608PhosphothreonineCombined sources1
Modified residuei633PhosphoserineCombined sources1
Modified residuei646PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q12256

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q12256

PRoteomics IDEntifications database

More...
PRIDEi
Q12256

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q12256

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

By transcription factor HAA1 in response to acetaldehyde accumulation.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
34662, 70 interactors

Database of interacting proteins

More...
DIPi
DIP-4096N

Protein interaction database and analysis system

More...
IntActi
Q12256, 22 interactors

Molecular INTeraction database

More...
MINTi
Q12256

STRING: functional protein association networks

More...
STRINGi
4932.YOR273C

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
Q12256

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000160689

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q12256

Identification of Orthologs from Complete Genome Data

More...
OMAi
VLFAFFE

Database of Orthologous Groups

More...
OrthoDBi
EOG092C2079

Family and domain databases

Conserved Domains Database

More...
CDDi
cd06174 MFS, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
IPR005829 Sugar_transporter_CS

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF07690 MFS_1, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF103473 SSF103473, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00216 SUGAR_TRANSPORT_1, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q12256-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPSSLTKTES NSDPRTNIQQ VPKALDKNVT NSGNLDSTSS STGSITEDEK
60 70 80 90 100
RSEPNADSNN MTGGEPIDPR DLDWDGPDDP DNPHNWSSLK KWYTTMTSAF
110 120 130 140 150
LCLVVTMGSS LYVSSVPELV ERYHVSQTLA LAGLTFYLLG LSTVIGAPLS
160 170 180 190 200
EVFGRKPVYL FSLPVSMLFT MGVGLSNGHM RIILPLRFLS GVFASPALSV
210 220 230 240 250
GSGTILDIFD VDQVSVAMTY FVLSPFLGPV LSPIMAGFAT EAKGWRWSEW
260 270 280 290 300
IQLIAGGLIL PFIALMPETH KGIILRKRAK KRNIALKKFS REAQKEFLKT
310 320 330 340 350
TVTITILRPL KMLVVEPIVF VFSVYVAFIF AILFGFFEAY AVIYRGVYHM
360 370 380 390 400
SMGISGLPFI GIGVGLWIGA FFYLYIDRKY LFPKPPAGTQ PLTEKERTSK
410 420 430 440 450
RTTPYRGARD AETGELLPVV PEKFLIACKF GSVALPIGLF WQAWTARSDV
460 470 480 490 500
HWMAPVAAGV PFGFGLILIF FSVLMYFSTC YPPLTVASCL AANNLLRYVM
510 520 530 540 550
SSVFPLFTIQ MYTKMKIKWA STLFALVCVV MIPIPWVFEK WGSKLRHKSQ
560 570 580 590 600
FGYAAMEKEA ETEGGIDDVN AVDGELNLTR MTTLRTMETD PSTREKPGER
610 620 630 640 650
LSLRRTHTQP VPASFDREDG QHAQNRNEPI SNSLYSAIKD NEDGYSYTEM

ATDASARMV
Length:659
Mass (Da):73,223
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i7F2BD0EEC5E408FC
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X89633 Genomic DNA Translation: CAA61779.1
Z75181 Genomic DNA Translation: CAA99498.1
BK006948 Genomic DNA Translation: DAA11039.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S67175

NCBI Reference Sequences

More...
RefSeqi
NP_014916.1, NM_001183692.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YOR273C_mRNA; YOR273C_mRNA; YOR273C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
854447

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YOR273C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89633 Genomic DNA Translation: CAA61779.1
Z75181 Genomic DNA Translation: CAA99498.1
BK006948 Genomic DNA Translation: DAA11039.1
PIRiS67175
RefSeqiNP_014916.1, NM_001183692.1

3D structure databases

ProteinModelPortaliQ12256
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34662, 70 interactors
DIPiDIP-4096N
IntActiQ12256, 22 interactors
MINTiQ12256
STRINGi4932.YOR273C

Protein family/group databases

TCDBi2.A.1.2.64 the major facilitator superfamily (mfs)

PTM databases

iPTMnetiQ12256

Proteomic databases

MaxQBiQ12256
PaxDbiQ12256
PRIDEiQ12256

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR273C_mRNA; YOR273C_mRNA; YOR273C
GeneIDi854447
KEGGisce:YOR273C

Organism-specific databases

SGDiS000005799 TPO4

Phylogenomic databases

HOGENOMiHOG000160689
InParanoidiQ12256
OMAiVLFAFFE
OrthoDBiEOG092C2079

Enzyme and pathway databases

BioCyciYEAST:G3O-33763-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q12256

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
IPR005829 Sugar_transporter_CS
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
PROSITEiView protein in PROSITE
PS00216 SUGAR_TRANSPORT_1, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTPO4_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q12256
Secondary accession number(s): D6W2X3
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: December 5, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names
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