UniProtKB - Q12250 (RPN5_YEAST)
Protein
26S proteasome regulatory subunit RPN5
Gene
RPN5
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.1 Publication
Miscellaneous
Present with 5710 molecules/cell in log phase SD medium.1 Publication
GO - Biological processi
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein deneddylation Source: SGD
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5689880, Ub-specific processing proteases R-SCE-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: 26S proteasome regulatory subunit RPN5Alternative name(s): Proteasome non-ATPase subunit 5 |
Gene namesi | Name:RPN5 Synonyms:NAS5 Ordered Locus Names:YDL147W ORF Names:D1572 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002306, RPN5 |
VEuPathDBi | FungiDB:YDL147W |
Subcellular locationi
Cytosol
- proteasome storage granule Source: SGD
Nucleus
- COP9 signalosome Source: SGD
- nuclear proteasome complex Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- proteasome complex Source: SGD
- proteasome regulatory particle, lid subcomplex Source: SGD
Keywords - Cellular componenti
ProteasomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000173865 | 2 – 445 | 26S proteasome regulatory subunit RPN5Add BLAST | 444 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserine1 Publication | 1 |
Post-translational modificationi
N-acetylated by NAT1.1 Publication
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | Q12250 |
PaxDbi | Q12250 |
PRIDEi | Q12250 |
PTM databases
iPTMneti | Q12250 |
Interactioni
Binary interactionsi
Hide detailsQ12250
Protein-protein interaction databases
BioGRIDi | 31914, 622 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-1578N |
IntActi | Q12250, 58 interactors |
MINTi | Q12250 |
STRINGi | 4932.YDL147W |
Miscellaneous databases
RNActi | Q12250, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q12250 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 233 – 407 | PCIPROSITE-ProRule annotationAdd BLAST | 175 |
Sequence similaritiesi
Belongs to the proteasome subunit p55 family.Curated
Phylogenomic databases
eggNOGi | KOG1498, Eukaryota |
GeneTreei | ENSGT00940000153510 |
HOGENOMi | CLU_033860_2_0_1 |
InParanoidi | Q12250 |
OMAi | MVQECCT |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR000717, PCI_dom IPR035297, PSMD12 IPR040134, PSMD12/CSN4 IPR040896, RPN5_C IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR10855, PTHR10855, 1 hit PTHR10855:SF1, PTHR10855:SF1, 1 hit |
Pfami | View protein in Pfam PF01399, PCI, 1 hit PF18098, RPN5_C, 1 hit |
SMARTi | View protein in SMART SM00088, PINT, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50250, PCI, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q12250-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRDAPIKAD KDYSQILKEE FPKIDSLAQN DCNSALDQLL VLEKKTRQAS
60 70 80 90 100
DLASSKEVLA KIVDLLASRN KWDDLNEQLT LLSKKHGQLK LSIQYMIQKV
110 120 130 140 150
MEYLKSSKSL DLNTRISVIE TIRVVTENKI FVEVERARVT KDLVEIKKEE
160 170 180 190 200
GKIDEAADIL CELQVETYGS MEMSEKIQFI LEQMELSILK GDYSQATVLS
210 220 230 240 250
RKILKKTFKN PKYESLKLEY YNLLVKISLH KREYLEVAQY LQEIYQTDAI
260 270 280 290 300
KSDEAKWKPV LSHIVYFLVL SPYGNLQNDL IHKIQNDNNL KKLESQESLV
310 320 330 340 350
KLFTTNELMR WPIVQKTYEP VLNEDDLAFG GEANKHHWED LQKRVIEHNL
360 370 380 390 400
RVISEYYSRI TLLRLNELLD LTESQTETYI SDLVNQGIIY AKVNRPAKIV
410 420 430 440
NFEKPKNSSQ LLNEWSHNVD ELLEHIETIG HLITKEEIMH GLQAK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97751 Genomic DNA Translation: CAA66344.1 Z74195 Genomic DNA Translation: CAA98721.1 BK006938 Genomic DNA Translation: DAA11711.1 |
PIRi | S67695 |
RefSeqi | NP_010134.1, NM_001180207.1 |
Genome annotation databases
EnsemblFungii | YDL147W_mRNA; YDL147W; YDL147W |
GeneIDi | 851408 |
KEGGi | sce:YDL147W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97751 Genomic DNA Translation: CAA66344.1 Z74195 Genomic DNA Translation: CAA98721.1 BK006938 Genomic DNA Translation: DAA11711.1 |
PIRi | S67695 |
RefSeqi | NP_010134.1, NM_001180207.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3J47 | electron microscopy | - | P | 409-442 | [»] | |
3JCK | electron microscopy | 3.50 | B | 1-445 | [»] | |
3JCO | electron microscopy | 4.80 | P | 1-445 | [»] | |
3JCP | electron microscopy | 4.60 | P | 1-445 | [»] | |
4CR2 | electron microscopy | 7.70 | P | 1-445 | [»] | |
4CR3 | electron microscopy | 9.30 | P | 1-445 | [»] | |
4CR4 | electron microscopy | 8.80 | P | 1-445 | [»] | |
5A5B | electron microscopy | 9.50 | P | 1-445 | [»] | |
5MPB | electron microscopy | 7.80 | P | 1-445 | [»] | |
5MPC | electron microscopy | 7.70 | P | 1-445 | [»] | |
5MPD | electron microscopy | 4.10 | P | 1-445 | [»] | |
5MPE | electron microscopy | 4.50 | P | 1-445 | [»] | |
5WVI | electron microscopy | 6.30 | P | 1-445 | [»] | |
5WVK | electron microscopy | 4.20 | P | 1-445 | [»] | |
5ZMR | NMR | - | A | 1-136 | [»] | |
6FVT | electron microscopy | 4.10 | P | 1-440 | [»] | |
6FVU | electron microscopy | 4.50 | P | 1-440 | [»] | |
6FVV | electron microscopy | 5.40 | P | 1-440 | [»] | |
6FVW | electron microscopy | 4.50 | P | 1-440 | [»] | |
6FVX | electron microscopy | 4.90 | P | 1-440 | [»] | |
6FVY | electron microscopy | 6.10 | P | 1-440 | [»] | |
6J2C | electron microscopy | 7.00 | P | 1-445 | [»] | |
6J2N | electron microscopy | 7.50 | P | 1-445 | [»] | |
6J2Q | electron microscopy | 3.80 | P | 1-445 | [»] | |
6J2X | electron microscopy | 3.80 | P | 1-445 | [»] | |
6J30 | electron microscopy | 4.50 | P | 1-445 | [»] | |
SMRi | Q12250 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31914, 622 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-1578N |
IntActi | Q12250, 58 interactors |
MINTi | Q12250 |
STRINGi | 4932.YDL147W |
PTM databases
iPTMneti | Q12250 |
Proteomic databases
MaxQBi | Q12250 |
PaxDbi | Q12250 |
PRIDEi | Q12250 |
Genome annotation databases
EnsemblFungii | YDL147W_mRNA; YDL147W; YDL147W |
GeneIDi | 851408 |
KEGGi | sce:YDL147W |
Organism-specific databases
SGDi | S000002306, RPN5 |
VEuPathDBi | FungiDB:YDL147W |
Phylogenomic databases
eggNOGi | KOG1498, Eukaryota |
GeneTreei | ENSGT00940000153510 |
HOGENOMi | CLU_033860_2_0_1 |
InParanoidi | Q12250 |
OMAi | MVQECCT |
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5689880, Ub-specific processing proteases R-SCE-6798695, Neutrophil degranulation |
Miscellaneous databases
PROi | PR:Q12250 |
RNActi | Q12250, protein |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR000717, PCI_dom IPR035297, PSMD12 IPR040134, PSMD12/CSN4 IPR040896, RPN5_C IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR10855, PTHR10855, 1 hit PTHR10855:SF1, PTHR10855:SF1, 1 hit |
Pfami | View protein in Pfam PF01399, PCI, 1 hit PF18098, RPN5_C, 1 hit |
SMARTi | View protein in SMART SM00088, PINT, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50250, PCI, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPN5_YEAST | |
Accessioni | Q12250Primary (citable) accession number: Q12250 Secondary accession number(s): D6VRK1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 15, 2002 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 174 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families