UniProtKB - Q12207 (NCE2_YEAST)
Non-classical export protein 2
NCE102
Functioni
Involved in membrane organization. Required for the formation of membrane compartments of CAN1 (MCCs), localization of CAN1 at the MCCs and subsequent invagination of the plasma membrane at the MCCs sites. Involved in eisosome organization and might act as a sensor of sphingolipids that regulates plasma membrane function. Involved in a novel pathway of export of proteins that lack a cleavable signal sequence. It may be an accessory subunit to an essential core component of the non-classical export machinery. Non-classical export pathway functions also as an alternative clearance/detoxification pathway to eliminate damaged material, when the basic repair pathway is not sufficient.
6 PublicationsGO - Biological processi
- eisosome assembly Source: SGD
- negative regulation of protein phosphorylation Source: SGD
- plasma membrane organization Source: SGD
- protein localization to plasma membrane Source: SGD
- protein secretion Source: SGD
- regulation of fungal-type cell wall organization Source: SGD
- regulation of phospholipid translocation Source: SGD
Keywordsi
Biological process | Protein transport, Transport |
Protein family/group databases
TCDBi | 8.A.148.1.1, the plasma membrane organizing center, eisosome (eisosome) family 9.A.27.1.1, the non-classical protein exporter (ncpe) family |
Names & Taxonomyi
Protein namesi | Recommended name: Non-classical export protein 2 |
Gene namesi | Name:NCE102 Synonyms:NCE2, RTG2S2 Ordered Locus Names:YPR149W ORF Names:P9659.2 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006353, NCE102 |
VEuPathDBi | FungiDB:YPR149W |
Subcellular locationi
Plasma membrane
- Cell membrane 5 Publications; Multi-pass membrane protein 5 Publications
Note: Associates with the ergosterol-rich membrane compartment of CAN1 (MCC). Accumulates in membrane domains at eisosomes.
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
Mitochondrion
- mitochondrion Source: SGD
Plasma Membrane
- cellular bud membrane Source: SGD
- mating projection membrane Source: SGD
- plasma membrane Source: SGD
Other locations
- cell periphery Source: SGD
- cytoplasm Source: SGD
- integral component of membrane Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 10 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 11 – 30 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 31 – 37 | ExtracellularSequence analysis | 7 | |
Transmembranei | 38 – 62 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 63 – 75 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 76 – 93 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 94 – 137 | ExtracellularSequence analysisAdd BLAST | 44 | |
Transmembranei | 138 – 157 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 158 – 173 | CytoplasmicSequence analysisAdd BLAST | 16 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096760 | 1 – 173 | Non-classical export protein 2Add BLAST | 173 |
Proteomic databases
MaxQBi | Q12207 |
PaxDbi | Q12207 |
PRIDEi | Q12207 |
TopDownProteomicsi | Q12207 |
PTM databases
iPTMneti | Q12207 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 36317, 68 interactors |
DIPi | DIP-5397N |
IntActi | Q12207, 31 interactors |
STRINGi | 4932.YPR149W |
Miscellaneous databases
RNActi | Q12207, protein |
Structurei
3D structure databases
AlphaFoldDBi | Q12207 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502RZW2, Eukaryota |
GeneTreei | ENSGT00940000176512 |
HOGENOMi | CLU_098356_1_0_1 |
InParanoidi | Q12207 |
OMAi | QGSGKRC |
Family and domain databases
InterProi | View protein in InterPro IPR008253, Marvel |
Pfami | View protein in Pfam PF01284, MARVEL, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MLALADNILR IINFLFLVIS IGLISSLLNT QHRHSSRVNY CMFACAYGIF
60 70 80 90 100
TDSLYGVFAN FIEPLAWPLV LFTLDFLNFV FTFTAGTVLA VGIRAHSCNN
110 120 130 140 150
SSYVDSNKIT QGSGTRCRQA QAAVAFLYFS CAIFLAKTLM SVFNMISNGA
160 170
FGSGSFSKRR RTGQVGVPTI SQV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U41659 mRNA Translation: AAB05589.1 U65683 Genomic DNA Translation: AAB06618.1 U40829 Genomic DNA Translation: AAB68287.1 AY558523 Genomic DNA Translation: AAS56849.1 BK006949 Genomic DNA Translation: DAA11562.1 |
PIRi | S69036 |
RefSeqi | NP_015475.1, NM_001184246.1 |
Genome annotation databases
EnsemblFungii | YPR149W_mRNA; YPR149W; YPR149W |
GeneIDi | 856272 |
KEGGi | sce:YPR149W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U41659 mRNA Translation: AAB05589.1 U65683 Genomic DNA Translation: AAB06618.1 U40829 Genomic DNA Translation: AAB68287.1 AY558523 Genomic DNA Translation: AAS56849.1 BK006949 Genomic DNA Translation: DAA11562.1 |
PIRi | S69036 |
RefSeqi | NP_015475.1, NM_001184246.1 |
3D structure databases
AlphaFoldDBi | Q12207 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 36317, 68 interactors |
DIPi | DIP-5397N |
IntActi | Q12207, 31 interactors |
STRINGi | 4932.YPR149W |
Protein family/group databases
TCDBi | 8.A.148.1.1, the plasma membrane organizing center, eisosome (eisosome) family 9.A.27.1.1, the non-classical protein exporter (ncpe) family |
PTM databases
iPTMneti | Q12207 |
Proteomic databases
MaxQBi | Q12207 |
PaxDbi | Q12207 |
PRIDEi | Q12207 |
TopDownProteomicsi | Q12207 |
Genome annotation databases
EnsemblFungii | YPR149W_mRNA; YPR149W; YPR149W |
GeneIDi | 856272 |
KEGGi | sce:YPR149W |
Organism-specific databases
SGDi | S000006353, NCE102 |
VEuPathDBi | FungiDB:YPR149W |
Phylogenomic databases
eggNOGi | ENOG502RZW2, Eukaryota |
GeneTreei | ENSGT00940000176512 |
HOGENOMi | CLU_098356_1_0_1 |
InParanoidi | Q12207 |
OMAi | QGSGKRC |
Miscellaneous databases
ChiTaRSi | NCE102, yeast |
PROi | PR:Q12207 |
RNActi | Q12207, protein |
Family and domain databases
InterProi | View protein in InterPro IPR008253, Marvel |
Pfami | View protein in Pfam PF01284, MARVEL, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NCE2_YEAST | |
Accessioni | Q12207Primary (citable) accession number: Q12207 Secondary accession number(s): D6W4E6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - SIMILARITY comments
Index of protein domains and families