UniProtKB - Q12186 (BBP_YEAST)
Protein
Branchpoint-bridging protein
Gene
MSL5
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. 2 commitment complexes, CC1 and CC2, have been defined. CC1 is a basal complex dependent only on the 5'-splice site. CC2 is a complex of lower mobility and is dependent on a branchpoint as well as a 5'-splice site region. This protein is involved in CC2 formation where it binds to the snRNP U1-associated protein PRP40, bridging the U1 snRNP-associated 5'-splice site and the MSL5-associated branch point 3' intron splice site. Involved in nuclear retention of pre-mRNA.3 Publications
Miscellaneous
Present with 4380 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 271 – 288 | CCHC-type 1PROSITE-ProRule annotationAdd BLAST | 18 | |
Zinc fingeri | 297 – 314 | CCHC-type 2PROSITE-ProRule annotationAdd BLAST | 18 |
GO - Molecular functioni
- mRNA binding Source: GO_Central
- pre-mRNA branch point binding Source: SGD
- zinc ion binding Source: InterPro
GO - Biological processi
- mRNA splicing, via spliceosome Source: SGD
- regulation of alternative mRNA splicing, via spliceosome Source: GO_Central
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Branchpoint-bridging proteinAlternative name(s): Mud synthetic-lethal 5 protein Splicing factor 1 Zinc finger protein BBP |
Gene namesi | Name:MSL5 Synonyms:BBP, SF1 Ordered Locus Names:YLR116W ORF Names:L2949 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YLR116W |
SGDi | S000004106, MSL5 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytosol
- cytosol Source: SGD
Nucleus
- commitment complex Source: SGD
- nucleus Source: SGD
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 60 | R → G in MSL5-2; temperature-sensitive; growth defect; when associated with N-72 and S-155. 1 Publication | 1 | |
Mutagenesisi | 72 | I → N in MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and S-155. 1 Publication | 1 | |
Mutagenesisi | 155 | P → S in MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and N-72. 1 Publication | 1 | |
Mutagenesisi | 195 | V → D in MSL5-5; cold-sensitive; growth defect; when associated with V-258. 1 Publication | 1 | |
Mutagenesisi | 230 | G → S in msl-5; loss of function. 1 Publication | 1 | |
Mutagenesisi | 258 | E → V in MSL5-5; cold-sensitive; growth defect; when associated with D-195. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000234564 | 1 – 476 | Branchpoint-bridging proteinAdd BLAST | 476 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 93 | PhosphoserineCombined sources | 1 | |
Modified residuei | 95 | PhosphoserineCombined sources | 1 | |
Modified residuei | 100 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 382 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q12186 |
PaxDbi | Q12186 |
PRIDEi | Q12186 |
PTM databases
iPTMneti | Q12186 |
Interactioni
Subunit structurei
Interacts with MUD2 and PRP40. The proline-rich sequence of this protein interacts with the GYF domains of SMY2 and SYH1.
3 PublicationsBinary interactionsi
Q12186
With | #Exp. | IntAct |
---|---|---|
MUD2 [P36084] | 5 | EBI-34012,EBI-11612 |
Protein-protein interaction databases
BioGRIDi | 31388, 262 interactors |
ComplexPortali | CPX-1417, BBP-MUD2 branchpoint-binding complex |
DIPi | DIP-991N |
IntActi | Q12186, 6 interactors |
MINTi | Q12186 |
STRINGi | 4932.YLR116W |
Miscellaneous databases
RNActi | Q12186, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q12186 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q12186 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 153 – 219 | KHAdd BLAST | 67 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 363 – 474 | Pro-richAdd BLAST | 112 |
Sequence similaritiesi
Belongs to the BBP/SF1 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 271 – 288 | CCHC-type 1PROSITE-ProRule annotationAdd BLAST | 18 | |
Zinc fingeri | 297 – 314 | CCHC-type 2PROSITE-ProRule annotationAdd BLAST | 18 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0119, Eukaryota |
GeneTreei | ENSGT00940000157258 |
HOGENOMi | CLU_016864_1_1_1 |
InParanoidi | Q12186 |
OMAi | HYNSNTH |
Family and domain databases
Gene3Di | 3.30.1370.10, 1 hit |
InterProi | View protein in InterPro IPR031150, BBP/SF1 IPR013041, Clathrin_app_Ig-like_sf IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR032570, SF1-HH IPR001878, Znf_CCHC IPR036875, Znf_CCHC_sf |
PANTHERi | PTHR11208:SF45, PTHR11208:SF45, 1 hit |
Pfami | View protein in Pfam PF00013, KH_1, 1 hit PF16275, SF1-HH, 1 hit PF00098, zf-CCHC, 1 hit |
SMARTi | View protein in SMART SM00322, KH, 1 hit SM00343, ZnF_C2HC, 2 hits |
SUPFAMi | SSF49348, SSF49348, 1 hit SSF54791, SSF54791, 1 hit SSF57756, SSF57756, 1 hit |
PROSITEi | View protein in PROSITE PS50158, ZF_CCHC, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12186-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSFRRINSRY FENRKGSSME EKKAKVPPNV NLSLWRKNTV ESDVHRFNSL
60 70 80 90 100
PSKISGALTR EQIYSYQVMF RIQEITIKLR TNDFVPPSRK NRSPSPPPVY
110 120 130 140 150
DAQGKRTNTR EQRYRKKLED ERIKLVEIAL KTIPYFVPPD DYKRPTKFQD
160 170 180 190 200
KYYIPVDQYP DVNFVGLLLG PRGRTLRKLQ EDSNCKIAIR GRGSVKEGKN
210 220 230 240 250
ASDLPPGAMN FEDPLHCLII ADSEDKIQKG IKVCQNIVIK AVTSPEGQND
260 270 280 290 300
LKRGQLRELA ELNGTLREDN RPCPICGLKD HKRYDCPNRK IPNIQGIVCK
310 320 330 340 350
ICGQTGHFSR DCNSSSQRMS RFDRNATVNN SAPIQSNDVH YNSNTHPIQA
360 370 380 390 400
PKRSRYDNNS TEPPLKFPAS SRYAPSPSPP ASHISRQAQN VTPTPPPGLT
410 420 430 440 450
SSSFSSGVPG IAPPPLQSPP ESEQPKFSLP PPPGMTTVQS SIAPPPGLSG
460 470
PPGFSNNMGN DINKPTPPGL QGPPGL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89514 Genomic DNA Translation: CAA61695.1 U53877 Genomic DNA Translation: AAB82363.1 Z73288 Genomic DNA Translation: CAA97683.1 BK006945 Genomic DNA Translation: DAA09430.1 |
PIRi | S64953 |
RefSeqi | NP_013217.1, NM_001182003.1 |
Genome annotation databases
EnsemblFungii | YLR116W_mRNA; YLR116W; YLR116W |
GeneIDi | 850807 |
KEGGi | sce:YLR116W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89514 Genomic DNA Translation: CAA61695.1 U53877 Genomic DNA Translation: AAB82363.1 Z73288 Genomic DNA Translation: CAA97683.1 BK006945 Genomic DNA Translation: DAA09430.1 |
PIRi | S64953 |
RefSeqi | NP_013217.1, NM_001182003.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FMA | X-ray | 2.50 | L/M/N/O/P | 440-450 | [»] | |
4WAL | X-ray | 2.20 | A | 144-271 | [»] | |
4WAN | X-ray | 1.80 | A/C/E/G | 144-271 | [»] | |
SMRi | Q12186 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31388, 262 interactors |
ComplexPortali | CPX-1417, BBP-MUD2 branchpoint-binding complex |
DIPi | DIP-991N |
IntActi | Q12186, 6 interactors |
MINTi | Q12186 |
STRINGi | 4932.YLR116W |
PTM databases
iPTMneti | Q12186 |
Proteomic databases
MaxQBi | Q12186 |
PaxDbi | Q12186 |
PRIDEi | Q12186 |
Genome annotation databases
EnsemblFungii | YLR116W_mRNA; YLR116W; YLR116W |
GeneIDi | 850807 |
KEGGi | sce:YLR116W |
Organism-specific databases
EuPathDBi | FungiDB:YLR116W |
SGDi | S000004106, MSL5 |
Phylogenomic databases
eggNOGi | KOG0119, Eukaryota |
GeneTreei | ENSGT00940000157258 |
HOGENOMi | CLU_016864_1_1_1 |
InParanoidi | Q12186 |
OMAi | HYNSNTH |
Miscellaneous databases
EvolutionaryTracei | Q12186 |
PROi | PR:Q12186 |
RNActi | Q12186, protein |
Family and domain databases
Gene3Di | 3.30.1370.10, 1 hit |
InterProi | View protein in InterPro IPR031150, BBP/SF1 IPR013041, Clathrin_app_Ig-like_sf IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR032570, SF1-HH IPR001878, Znf_CCHC IPR036875, Znf_CCHC_sf |
PANTHERi | PTHR11208:SF45, PTHR11208:SF45, 1 hit |
Pfami | View protein in Pfam PF00013, KH_1, 1 hit PF16275, SF1-HH, 1 hit PF00098, zf-CCHC, 1 hit |
SMARTi | View protein in SMART SM00322, KH, 1 hit SM00343, ZnF_C2HC, 2 hits |
SUPFAMi | SSF49348, SSF49348, 1 hit SSF54791, SSF54791, 1 hit SSF57756, SSF57756, 1 hit |
PROSITEi | View protein in PROSITE PS50158, ZF_CCHC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BBP_YEAST | |
Accessioni | Q12186Primary (citable) accession number: Q12186 Secondary accession number(s): D6VYB4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD