Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homocitrate synthase, mitochondrial

Gene

LYS21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 21900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + H2O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA.

Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 1 of the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Homocitrate synthase, cytosolic isozyme (LYS20), Homocitrate synthase, mitochondrial (LYS21)
  2. Homocitrate dehydratase, mitochondrial (ACO2)
  3. Homoaconitase, mitochondrial (LYS4)
  4. Homoisocitrate dehydrogenase, mitochondrial (LYS12)
  5. Aromatic/aminoadipate aminotransferase 1 (ARO8)
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate, the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • homocitrate synthase activity Source: SGD

GO - Biological processi

  • lysine biosynthetic process via aminoadipic acid Source: SGD

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:YDL131W-MONOMER
YEAST:YDL131W-MONOMER
BRENDAi2.3.3.14 984
SABIO-RKiQ12122
UniPathwayiUPA00033; UER00028

Names & Taxonomyi

Protein namesi
Recommended name:
Homocitrate synthase, mitochondrial (EC:2.3.3.14)
Gene namesi
Name:LYS21
Ordered Locus Names:YDL131W
ORF Names:D2195
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL131W
SGDiS000002289 LYS21

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000001051? – 440Homocitrate synthase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei399PhosphoserineBy similarity1
Modified residuei410PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12122
PaxDbiQ12122
PRIDEiQ12122

PTM databases

iPTMnetiQ12122

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LYS20P4857051EBI-8508,EBI-8502

Protein-protein interaction databases

BioGridi31931, 61 interactors
DIPiDIP-4634N
IntActiQ12122, 23 interactors
MINTiQ12122
STRINGi4932.YDL131W

Structurei

3D structure databases

ProteinModelPortaliQ12122
SMRiQ12122
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 290Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000046858
InParanoidiQ12122
KOiK01655
OMAiKFHGVGL
OrthoDBiEOG092C2OYT

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR002034 AIPM/Hcit_synth_CS
IPR013785 Aldolase_TIM
IPR011872 Homocitrate_synth_fun/arc
IPR000891 PYR_CT
PfamiView protein in Pfam
PF00682 HMGL-like, 1 hit
TIGRFAMsiTIGR02146 LysS_fung_arch, 1 hit
PROSITEiView protein in PROSITE
PS00815 AIPM_HOMOCIT_SYNTH_1, 1 hit
PS00816 AIPM_HOMOCIT_SYNTH_2, 1 hit
PS50991 PYR_CT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSENNEFQSV TESTTAPTTS NPYGPNPADY LSNVKNFQLI DSTLREGEQF
60 70 80 90 100
ANAFFDTEKK IEIARALDDF GVDYIELTSP VASEQSRKDC EAICKLGLKA
110 120 130 140 150
KILTHIRCHM DDARVAVETG VDGVDVVIGT SKFLRQYSHG KDMNYIAKSA
160 170 180 190 200
VEVIEFVKSK GIEIRFSSED SFRSDLVDLL NIYKTVDKIG VNRVGIADTV
210 220 230 240 250
GCANPRQVYE LIRTLKSVVS CDIECHFHND TGCAIANAYT ALEGGARLID
260 270 280 290 300
VSVLGIGERN GITPLGGLMA RMIVAAPDYV RSKYKLHKIR DIENLVADAV
310 320 330 340 350
EVNIPFNNPI TGFCAFTHKA GIHAKAILAN PSTYEILDPH DFGMKRYIHF
360 370 380 390 400
ANRLTGWNAI KSRVDQLNLN LTDDQIKEVT AKIKKLGDVR PLNIDDVDSI
410 420 430 440
IKDFHAELST PLLKPVNKGT DDDNIDISNG HVSKKAKVTK
Length:440
Mass (Da):48,594
Last modified:November 1, 1997 - v1
Checksum:i6B01827D998FD9AD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti199T → A in AAT92960 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96876 Genomic DNA Translation: CAA65629.1
Z74179 Genomic DNA Translation: CAA98700.1
AY692941 Genomic DNA Translation: AAT92960.1
BK006938 Genomic DNA Translation: DAA11728.1
PIRiS67674
RefSeqiNP_010151.1, NM_001180190.1

Genome annotation databases

EnsemblFungiiYDL131W; YDL131W; YDL131W
GeneIDi851425
KEGGisce:YDL131W

Similar proteinsi

Entry informationi

Entry nameiHOSM_YEAST
AccessioniPrimary (citable) accession number: Q12122
Secondary accession number(s): D6VRL8, E9P8Z7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 18, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health