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Protein

DNA mismatch repair protein MLH3

Gene

MLH3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in DNA mismatch repair (MMR), correcting insertion-deletion loops (IDLs) resulting from DNA replication, DNA damage or from recombination events between non-identical sequences during meiosis. Component of the MutLbeta heterodimer, which probably forms a ternary complex with the MutSbeta heterodimer that initially recognizes the DNA mismatches. This complex is thought to be responsible for directing the downsteam MMR events, including strand discrimination, excision, and resynthesis. Plays a major role in promoting meiotic crossing-over and is involved in maintaining the genetic stability of simple sequence repeats by correction of frameshift intermediates.3 Publications

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: InterPro
  • mismatched DNA binding Source: InterPro
  • single-stranded DNA binding Source: GO_Central

GO - Biological processi

  • meiotic mismatch repair Source: SGD
  • mismatch repair Source: SGD
  • reciprocal meiotic recombination Source: SGD

Keywordsi

Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-34060-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MLH3
Alternative name(s):
MutL protein homolog 3
Gene namesi
Name:MLH3
Ordered Locus Names:YPL164C
ORF Names:P2550
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL164C
SGDiS000006085 MLH3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002455701 – 715DNA mismatch repair protein MLH3Add BLAST715

Proteomic databases

MaxQBiQ12083
PaxDbiQ12083
PRIDEiQ12083

Interactioni

Subunit structurei

Heterodimer of MLH1 and MLH3, called MutLbeta, which is involved in correction of a specific subset of IDLs when associated with MutSbeta. Forms a ternary complex with a SGS1-TOP3 heterodimer during meiosis.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36019, 66 interactors
ComplexPortaliCPX-1668 MLH1-MLH3 complex
DIPiDIP-2414N
IntActiQ12083, 23 interactors
STRINGi4932.YPL164C

Structurei

3D structure databases

ProteinModelPortaliQ12083
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00800000124176
HOGENOMiHOG000113600
InParanoidiQ12083
KOiK08739
OMAiMPVRVKS
OrthoDBiEOG092C0TC4

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR013507 DNA_mismatch_S5_2-like
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR028830 Mlh3
IPR038973 MutL/Mlh/Pms
IPR014790 MutL_C
IPR037198 MutL_C_sf
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR10073 PTHR10073, 1 hit
PTHR10073:SF7 PTHR10073:SF7, 1 hit
SMARTiView protein in SMART
SM01340 DNA_mis_repair, 1 hit
SM00853 MutL_C, 1 hit
SUPFAMiSSF118116 SSF118116, 2 hits
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit

Sequencei

Sequence statusi: Complete.

Q12083-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQHIRKLDS DVSERLKSQA CTVSLASAVR EIVQNSVDAH ATTIDVMIDL
60 70 80 90 100
PNLSFAVYDD GIGLTRSDLN ILATQNYTSK IRKMNDLVTM KTYGYRGDAL
110 120 130 140 150
YSISNVSNLF VCSKKKDYNS AWMRKFPSKS VMLSENTILP IDPFWKICPW
160 170 180 190 200
SRTKSGTVVI VEDMLYNLPV RRRILKEEPP FKTFNTIKAD MLQILVMHPM
210 220 230 240 250
ISLNVQYTDK LRINTEVLFR SKNITEGLTK HQQMSQVLRN VFGAIIPPDM
260 270 280 290 300
LKKVSLKFNE YQIEGIISKM PVGLKDLQFI YINGRRYADS AFQGYVDSLF
310 320 330 340 350
QAQDFGEKGM SLLKTKSVGK PYRSHPVFIL DVRCPQTIDD LLQDPAKKIV
360 370 380 390 400
KPSHIRTIEP LIVKTIRSFL TFQGYLTPDK SDSSFEIVNC SQKTATLPDS
410 420 430 440 450
RIQISKRNQV LNSKMKIARI NSYIGKPAVN GCRINNSTIN YEKIKNIRID
460 470 480 490 500
GQKSRLRNKL SSRPYDSGFT EDYDSIGKTI TDFSISRSVL AKYEVINQVD
510 520 530 540 550
KKFILIRCLD QSIHNCPLLV LVDQHACDER IRLEELFYSL LTEVVTGTFV
560 570 580 590 600
ARDLKDCCIE VDRTEADLFK HYQSEFKKWG IGYETIEGTM ETSLLEIKTL
610 620 630 640 650
PEMLTSKYNG DKDYLKMVLL QHAHDLKDFK KLPMDLSHFE NYTSVDKLYW
660 670 680 690 700
WKYSSCVPTV FHEILNSKAC RSAVMFGDEL TRQECIILIS KLSRCHNPFE
710
CAHGRPSMVP IAELK
Length:715
Mass (Da):82,001
Last modified:November 1, 1996 - v1
Checksum:i970FD7F57EB6E3B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA Translation: CAA65557.1
Z73520 Genomic DNA Translation: CAA97869.1
BK006949 Genomic DNA Translation: DAA11270.1
PIRiS65175
RefSeqiNP_015161.1, NM_001183978.1

Genome annotation databases

EnsemblFungiiYPL164C; YPL164C; YPL164C
GeneIDi855939
KEGGisce:YPL164C

Similar proteinsi

Entry informationi

Entry nameiMLH3_YEAST
AccessioniPrimary (citable) accession number: Q12083
Secondary accession number(s): D6W3K4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 1, 1996
Last modified: July 18, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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