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UniProtKB - Q12063 (UPPS_YEAST)
Protein
Dehydrodolichyl diphosphate synthase complex subunit NUS1
Gene
NUS1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
With SRT1 or RER2, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Adds multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER).
1 PublicationCatalytic activityi
- (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = di-trans,poly-cis-polyprenyl diphosphate + n diphosphate1 PublicationEC:2.5.1.871 Publication
Cofactori
Mg2+By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.CuratedView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
GO - Biological processi
- dolichol biosynthetic process Source: SGD
- protein glycosylation Source: SGD
Keywordsi
Molecular function | Transferase |
Ligand | Magnesium |
Enzyme and pathway databases
BRENDAi | 2.5.1.87, 984 |
Reactomei | R-SCE-446199, Synthesis of Dolichyl-phosphate |
UniPathwayi | UPA00378 |
Names & Taxonomyi
Protein namesi | Recommended name: Dehydrodolichyl diphosphate synthase complex subunit NUS1Curated (EC:2.5.1.871 Publication)Alternative name(s): Nuclear undecaprenyl pyrophosphate synthase 1Imported |
Gene namesi | Name:NUS1 Ordered Locus Names:YDL193WImported ORF Names:D12391 Publication |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002352, NUS1 |
VEuPathDBi | FungiDB:YDL193W |
Subcellular locationi
Nucleus
- Nucleus membrane 1 Publication; Single-pass membrane protein Curated
Endoplasmic reticulum
- Endoplasmic reticulum membrane Curated; Single-pass membrane protein Curated
Other locations
- Lipid droplet 4 Publications
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: GO_Central
Nucleus
- nuclear envelope Source: SGD
- nuclear membrane Source: UniProtKB-SubCell
Other locations
- dehydrodolichyl diphosphate synthase complex Source: SGD
- integral component of membrane Source: UniProtKB-KW
- lipid droplet Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 97 – 119 | HelicalSequence analysisAdd BLAST | 23 |
Keywords - Cellular componenti
Endoplasmic reticulum, Lipid droplet, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 372 | N → H: Loss of function. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000242134 | 1 – 375 | Dehydrodolichyl diphosphate synthase complex subunit NUS1Add BLAST | 375 |
Proteomic databases
MaxQBi | Q12063 |
PaxDbi | Q12063 |
PRIDEi | Q12063 |
PTM databases
iPTMneti | Q12063 |
Interactioni
Subunit structurei
Forms an active dehydrodolichyl diphosphate synthase complex with either SRT1 or RER2.
1 PublicationProtein-protein interaction databases
BioGRIDi | 31852, 286 interactors |
ComplexPortali | CPX-162, Dehydrodolichyl diphosphate synthase complex variant RER2 CPX-166, Dehydrodolichyl diphosphate synthase complex variant SRT1 |
DIPi | DIP-1220N |
IntActi | Q12063, 13 interactors |
STRINGi | 4932.YDL193W |
Miscellaneous databases
RNActi | Q12063, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q12063 |
SMRi | Q12063 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 48 | DisorderedSequence analysisAdd BLAST | 48 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 32 | Basic and acidic residuesSequence analysisAdd BLAST | 32 |
Sequence similaritiesi
Belongs to the UPP synthase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2818, Eukaryota |
GeneTreei | ENSGT00390000003223 |
HOGENOMi | CLU_051870_0_0_1 |
InParanoidi | Q12063 |
OMAi | MPRVYEA |
Family and domain databases
Gene3Di | 3.40.1180.10, 1 hit |
InterProi | View protein in InterPro IPR038887, Nus1/NgBR IPR036424, UPP_synth-like_sf |
PANTHERi | PTHR21528, PTHR21528, 1 hit |
SUPFAMi | SSF64005, SSF64005, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12063-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPTMIKKDDK AMEPPNEKPH RKIERDDVPE SSNHIPPPES GVLKGGKVNS
60 70 80 90 100
KTRALKAVTS IIADADENPQ KKVNNETNGV QKQKTEDLSK RIGKFEYLFY
110 120 130 140 150
KFLLVLLYIC FGLFRYGQYQ YNKMKLRIFS IIYNHAYTPQ LIRQDVIPLK
160 170 180 190 200
KIPKRLAAIL EVKPVGDVGG GVTGLLNDAS EIVCWTVSAG IKHLMLYDYD
210 220 230 240 250
GILQRNVPEL RMEIHSNLAK YFGPAHVPNY AVKIPHSNKI FYNLDGIETE
260 270 280 290 300
TDVGNEIEAN QEKDKIAIEI SLLSNRDGRE TIVDLTKTMA ELCAVNELSV
310 320 330 340 350
SDITMDLVDS ELKQLVGPEP DLLLYFGPSL DLQGFPPWHI RLTEFYWEKD
360 370
NNEVIYSVFI RGLRQYAGCK VNVGK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83276 Genomic DNA Translation: CAA58254.1 Z74241 Genomic DNA Translation: CAA98770.1 BK006938 Genomic DNA Translation: DAA11670.1 |
PIRi | S58784 |
RefSeqi | NP_010088.1, NM_001180253.1 |
Genome annotation databases
EnsemblFungii | YDL193W_mRNA; YDL193W; YDL193W |
GeneIDi | 851334 |
KEGGi | sce:YDL193W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83276 Genomic DNA Translation: CAA58254.1 Z74241 Genomic DNA Translation: CAA98770.1 BK006938 Genomic DNA Translation: DAA11670.1 |
PIRi | S58784 |
RefSeqi | NP_010088.1, NM_001180253.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6JCN | X-ray | 2.00 | A/B | 148-375 | [»] | |
AlphaFoldDBi | Q12063 | |||||
SMRi | Q12063 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31852, 286 interactors |
ComplexPortali | CPX-162, Dehydrodolichyl diphosphate synthase complex variant RER2 CPX-166, Dehydrodolichyl diphosphate synthase complex variant SRT1 |
DIPi | DIP-1220N |
IntActi | Q12063, 13 interactors |
STRINGi | 4932.YDL193W |
PTM databases
iPTMneti | Q12063 |
Proteomic databases
MaxQBi | Q12063 |
PaxDbi | Q12063 |
PRIDEi | Q12063 |
Genome annotation databases
EnsemblFungii | YDL193W_mRNA; YDL193W; YDL193W |
GeneIDi | 851334 |
KEGGi | sce:YDL193W |
Organism-specific databases
SGDi | S000002352, NUS1 |
VEuPathDBi | FungiDB:YDL193W |
Phylogenomic databases
eggNOGi | KOG2818, Eukaryota |
GeneTreei | ENSGT00390000003223 |
HOGENOMi | CLU_051870_0_0_1 |
InParanoidi | Q12063 |
OMAi | MPRVYEA |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.5.1.87, 984 |
Reactomei | R-SCE-446199, Synthesis of Dolichyl-phosphate |
Miscellaneous databases
PROi | PR:Q12063 |
RNActi | Q12063, protein |
Family and domain databases
Gene3Di | 3.40.1180.10, 1 hit |
InterProi | View protein in InterPro IPR038887, Nus1/NgBR IPR036424, UPP_synth-like_sf |
PANTHERi | PTHR21528, PTHR21528, 1 hit |
SUPFAMi | SSF64005, SSF64005, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UPPS_YEAST | |
Accessioni | Q12063Primary (citable) accession number: Q12063 Secondary accession number(s): D6VRG0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families