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UniProtKB - Q12051 (GGPPS_YEAST)
Protein
Geranylgeranyl pyrophosphate synthase BTS1
Gene
BTS1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the trans-addition of the 3 molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate. Required for the membrane attachment of YPT1 and SEC4. May be involved in vesicle trafficking and protein sorting.
2 PublicationsMiscellaneous
Present with 2840 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:2.5.1.1
- EC:2.5.1.10
- (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = (2E,6E,10E)-geranylgeranyl diphosphate + diphosphate1 PublicationEC:2.5.1.291 Publication
Cofactori
Mg2+1 PublicationNote: Binds 2 Mg2+ ions per subunit.1 Publication
Kineticsi
- KM=3.2 µM for farnesyl diphosphate
- KM=0.8 µM for isopentenyl diphosphate
: farnesyl diphosphate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate. This subpathway is part of the pathway farnesyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate, the pathway farnesyl diphosphate biosynthesis and in Isoprenoid biosynthesis.
Pathwayi: geranyl diphosphate biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate. This subpathway is part of the pathway geranyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate, the pathway geranyl diphosphate biosynthesis and in Isoprenoid biosynthesis.
Pathwayi: geranylgeranyl diphosphate biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate. This subpathway is part of the pathway geranylgeranyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate, the pathway geranylgeranyl diphosphate biosynthesis and in Isoprenoid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 36 | Isopentenyl diphosphate1 Publication | 1 | |
Binding sitei | 39 | Isopentenyl diphosphate1 Publication | 1 | |
Binding sitei | 68 | Isopentenyl diphosphate1 Publication | 1 | |
Metal bindingi | 75 | Magnesium 12 Publications | 1 | |
Metal bindingi | 75 | Magnesium 22 Publications | 1 | |
Metal bindingi | 79 | Magnesium 12 Publications | 1 | |
Metal bindingi | 79 | Magnesium 22 Publications | 1 | |
Binding sitei | 84 | Dimethylallyl diphosphate1 Publication | 1 | |
Binding sitei | 85 | Isopentenyl diphosphate1 Publication | 1 | |
Sitei | 107 | Important for determining product chain length | 1 | |
Binding sitei | 169 | Dimethylallyl diphosphate1 Publication | 1 | |
Binding sitei | 170 | Dimethylallyl diphosphate1 Publication | 1 | |
Binding sitei | 206 | Dimethylallyl diphosphate1 Publication | 1 | |
Binding sitei | 213 | Dimethylallyl diphosphate1 Publication | 1 | |
Binding sitei | 223 | Dimethylallyl diphosphate1 Publication | 1 | |
Binding sitei | 233 | Dimethylallyl diphosphate1 Publication | 1 |
GO - Molecular functioni
- dimethylallyltranstransferase activity Source: UniProtKB-EC
- farnesyltranstransferase activity Source: SGD
- geranyltranstransferase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- prenyltransferase activity Source: GO_Central
GO - Biological processi
- farnesyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
- geranyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
- geranylgeranyl diphosphate biosynthetic process Source: CACAO
- isoprenoid biosynthetic process Source: GO_Central
- protein transport Source: UniProtKB-KW
- terpenoid biosynthetic process Source: SGD
Keywordsi
Molecular function | Transferase |
Biological process | Carotenoid biosynthesis, Isoprene biosynthesis, Protein transport, Transport |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.5.1.29, 984 |
Reactomei | R-SCE-191273, Cholesterol biosynthesis |
SABIO-RKi | Q12051 |
UniPathwayi | UPA00259;UER00368 UPA00260;UER00369 UPA00389;UER00564 |
Names & Taxonomyi
Protein namesi | Recommended name: Geranylgeranyl pyrophosphate synthase BTS11 Publication (EC:2.5.1.-)Short name: GGPP synthase Short name: GGPPSase Alternative name(s): |
Gene namesi | Name:BTS1 Ordered Locus Names:YPL069C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005990, BTS1 |
VEuPathDBi | FungiDB:YPL069C |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Mitochondrion
- mitochondrion Source: SGD
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 7 | E → G: No effect. Monomer; when associated with G-8. 1 Publication | 1 | |
Mutagenesisi | 8 | L → G: Monomer and homodimer. Monomer; when associated with G-7. 1 Publication | 1 | |
Mutagenesisi | 9 | I → G: Mostly monomer. Exclusively monomer; when associated with G-8. Reduces enzyme activity 1000-fold. 1 Publication | 1 | |
Mutagenesisi | 107 | Y → A: Reduced affinity for isopentenyl diphosphate (IPP). | 1 | |
Mutagenesisi | 108 | F → A: Reduced affinity for isopentenyl diphosphate (IPP). | 1 | |
Mutagenesisi | 139 | H → A: Reduced affinity for isopentenyl diphosphate (IPP). | 1 |
Chemistry databases
ChEMBLi | CHEMBL1075251 |
DrugCentrali | Q12051 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000228139 | 1 – 335 | Geranylgeranyl pyrophosphate synthase BTS1Add BLAST | 335 |
Proteomic databases
MaxQBi | Q12051 |
PaxDbi | Q12051 |
PRIDEi | Q12051 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 36110, 1026 interactors |
DIPi | DIP-8889N |
IntActi | Q12051, 5 interactors |
STRINGi | 4932.YPL069C |
Chemistry databases
BindingDBi | Q12051 |
Miscellaneous databases
RNActi | Q12051, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q12051 |
SMRi | Q12051 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q12051 |
Family & Domainsi
Sequence similaritiesi
Belongs to the FPP/GGPP synthase family.Curated
Phylogenomic databases
eggNOGi | KOG0777, Eukaryota |
GeneTreei | ENSGT00940000153498 |
HOGENOMi | CLU_014015_6_0_1 |
InParanoidi | Q12051 |
OMAi | FYSKAFF |
Family and domain databases
CDDi | cd00685, Trans_IPPS_HT, 1 hit |
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR000092, Polyprenyl_synt IPR033749, Polyprenyl_synt_CS |
Pfami | View protein in Pfam PF00348, polyprenyl_synt, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
PROSITEi | View protein in PROSITE PS00723, POLYPRENYL_SYNTHASE_1, 1 hit PS00444, POLYPRENYL_SYNTHASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12051-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEAKIDELIN NDPVWSSQNE SLISKPYNHI LLKPGKNFRL NLIVQINRVM
60 70 80 90 100
NLPKDQLAIV SQIVELLHNS SLLIDDIEDN APLRRGQTTS HLIFGVPSTI
110 120 130 140 150
NTANYMYFRA MQLVSQLTTK EPLYHNLITI FNEELINLHR GQGLDIYWRD
160 170 180 190 200
FLPEIIPTQE MYLNMVMNKT GGLFRLTLRL MEALSPSSHH GHSLVPFINL
210 220 230 240 250
LGIIYQIRDD YLNLKDFQMS SEKGFAEDIT EGKLSFPIVH ALNFTKTKGQ
260 270 280 290 300
TEQHNEILRI LLLRTSDKDI KLKLIQILEF DTNSLAYTKN FINQLVNMIK
310 320 330
NDNENKYLPD LASHSDTATN LHDELLYIID HLSEL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39205 Genomic DNA Translation: AAB68296.1 U31632 Genomic DNA Translation: AAA83262.1 AY692852 Genomic DNA Translation: AAT92871.1 BK006949 Genomic DNA Translation: DAA11362.1 |
PIRi | S60921 |
RefSeqi | NP_015256.1, NM_001183883.1 |
Genome annotation databases
EnsemblFungii | YPL069C_mRNA; YPL069C; YPL069C |
GeneIDi | 856036 |
KEGGi | sce:YPL069C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39205 Genomic DNA Translation: AAB68296.1 U31632 Genomic DNA Translation: AAA83262.1 AY692852 Genomic DNA Translation: AAT92871.1 BK006949 Genomic DNA Translation: DAA11362.1 |
PIRi | S60921 |
RefSeqi | NP_015256.1, NM_001183883.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DH4 | X-ray | 1.98 | A/B | 1-335 | [»] | |
2E8T | X-ray | 2.13 | A/B | 1-335 | [»] | |
2E8U | X-ray | 2.08 | A/B | 1-335 | [»] | |
2E8V | X-ray | 1.80 | A/B | 1-335 | [»] | |
2E8W | X-ray | 2.35 | A/B | 1-335 | [»] | |
2E8X | X-ray | 2.04 | A/B | 1-335 | [»] | |
2E90 | X-ray | 2.55 | A/B | 1-335 | [»] | |
2E91 | X-ray | 2.14 | A/B | 1-335 | [»] | |
2E92 | X-ray | 2.31 | A/B | 1-335 | [»] | |
2E93 | X-ray | 2.12 | A/B | 1-335 | [»] | |
2E94 | X-ray | 2.18 | A/B | 1-335 | [»] | |
2E95 | X-ray | 2.20 | A/B | 1-335 | [»] | |
2Z4V | X-ray | 1.86 | A/B | 1-335 | [»] | |
2Z4W | X-ray | 2.45 | A/B | 1-335 | [»] | |
2Z4X | X-ray | 1.90 | A/B | 1-335 | [»] | |
2Z4Y | X-ray | 2.10 | A/B | 1-335 | [»] | |
2Z4Z | X-ray | 2.09 | A/B | 1-335 | [»] | |
2Z50 | X-ray | 2.01 | A/B | 1-335 | [»] | |
2Z52 | X-ray | 2.13 | A/B | 1-335 | [»] | |
2Z78 | X-ray | 2.10 | A/B | 1-335 | [»] | |
2Z7H | X-ray | 2.08 | A/B | 1-335 | [»] | |
2Z7I | X-ray | 2.10 | A/B | 1-335 | [»] | |
2ZEU | X-ray | 2.00 | A/B | 1-335 | [»] | |
2ZEV | X-ray | 2.23 | A/B | 1-335 | [»] | |
AlphaFoldDBi | Q12051 | |||||
SMRi | Q12051 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36110, 1026 interactors |
DIPi | DIP-8889N |
IntActi | Q12051, 5 interactors |
STRINGi | 4932.YPL069C |
Chemistry databases
BindingDBi | Q12051 |
ChEMBLi | CHEMBL1075251 |
DrugCentrali | Q12051 |
Proteomic databases
MaxQBi | Q12051 |
PaxDbi | Q12051 |
PRIDEi | Q12051 |
Genome annotation databases
EnsemblFungii | YPL069C_mRNA; YPL069C; YPL069C |
GeneIDi | 856036 |
KEGGi | sce:YPL069C |
Organism-specific databases
SGDi | S000005990, BTS1 |
VEuPathDBi | FungiDB:YPL069C |
Phylogenomic databases
eggNOGi | KOG0777, Eukaryota |
GeneTreei | ENSGT00940000153498 |
HOGENOMi | CLU_014015_6_0_1 |
InParanoidi | Q12051 |
OMAi | FYSKAFF |
Enzyme and pathway databases
UniPathwayi | UPA00259;UER00368 UPA00260;UER00369 UPA00389;UER00564 |
BRENDAi | 2.5.1.29, 984 |
Reactomei | R-SCE-191273, Cholesterol biosynthesis |
SABIO-RKi | Q12051 |
Miscellaneous databases
EvolutionaryTracei | Q12051 |
PROi | PR:Q12051 |
RNActi | Q12051, protein |
Family and domain databases
CDDi | cd00685, Trans_IPPS_HT, 1 hit |
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR000092, Polyprenyl_synt IPR033749, Polyprenyl_synt_CS |
Pfami | View protein in Pfam PF00348, polyprenyl_synt, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
PROSITEi | View protein in PROSITE PS00723, POLYPRENYL_SYNTHASE_1, 1 hit PS00444, POLYPRENYL_SYNTHASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GGPPS_YEAST | |
Accessioni | Q12051Primary (citable) accession number: Q12051 Secondary accession number(s): D6W3U6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 21, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families