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Protein

Transcriptional regulator MET32

Gene

MET32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Auxiliary transcriptional regulator of sulfur amino acid metabolism. Involved in the transcriptional activation of MET28.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri98 – 120C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri126 – 150C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • proximal promoter sequence-specific DNA binding Source: SGD
  • RNA polymerase II activating transcription factor binding Source: SGD
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: SGD

GO - Biological processi

  • negative regulation of transcription by RNA polymerase II Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • regulation of mitotic cell cycle Source: SGD
  • regulation of sulfur amino acid metabolic process Source: SGD
  • regulation of transcription by RNA polymerase II Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29825-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulator MET32
Alternative name(s):
Methionine-requiring protein 32
Gene namesi
Name:MET32
Ordered Locus Names:YDR253C
ORF Names:YD9320A.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR253C
SGDiS000002661 MET32

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000468091 – 191Transcriptional regulator MET32Add BLAST191

Proteomic databases

MaxQBiQ12041
PaxDbiQ12041
PRIDEiQ12041

Interactioni

Subunit structurei

Interacts with MET4 and MET28.1 Publication

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi32303, 90 interactors
ComplexPortaliCPX-1015 MET4-MET28-MET32 sulfur metabolism transcription factor complex
DIPiDIP-2411N
IntActiQ12041, 2 interactors
STRINGi4932.YDR253C

Structurei

3D structure databases

ProteinModelPortaliQ12041
SMRiQ12041
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri98 – 120C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri126 – 150C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00730000113983
HOGENOMiHOG000000993
InParanoidiQ12041
OMAiTCKRNRQ
OrthoDBiEOG092C35YC

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 2 hits
SUPFAMiSSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 2 hits

Sequencei

Sequence statusi: Complete.

Q12041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDQDAAFIK QATEAIVDVS LNIDNIDPII KELLERVRNR QNRLQNKKPA
60 70 80 90 100
LIPAENGVDI NSQGGNIKVK KENALPKPPK SSKSKPQDRR NSTGEKRFKC
110 120 130 140 150
AKCSLEFSRS SDLRRHEKTH FAILPNICPQ CGKGFARKDA LKRHYDTLTC
160 170 180 190
RRNRTKLLTA GGEGINELLK KVKQSNIVHR QDNNHNGSSN G
Length:191
Mass (Da):21,518
Last modified:November 1, 1996 - v1
Checksum:i3A7BC87893311664
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70202 Genomic DNA Translation: CAA94092.1
Z68329 Genomic DNA Translation: CAA92710.1
AY557729 Genomic DNA Translation: AAS56055.1
BK006938 Genomic DNA Translation: DAA12093.1
PIRiS67310
RefSeqiNP_010539.1, NM_001180561.1

Genome annotation databases

EnsemblFungiiYDR253C; YDR253C; YDR253C
GeneIDi851840
KEGGisce:YDR253C

Similar proteinsi

Entry informationi

Entry nameiMET32_YEAST
AccessioniPrimary (citable) accession number: Q12041
Secondary accession number(s): D6VSN3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 18, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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