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UniProtKB - Q12033 (PALA_YEAST)
Protein
pH-response regulator protein palA/RIM20
Gene
RIM20
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Required for the proteolytic cleavage of the transcriptional repressor RIM101 in response to alkaline ambient pH, which is necessary for sporulation and invasive growth. May act as a scaffold protein that recruits the calpain-like protease RIM13 via SNF7 to its substrate RIM101.
1 PublicationMiscellaneous
Present with 2881 molecules/cell in log phase SD medium.1 Publication
GO - Biological processi
- invasive growth in response to glucose limitation Source: SGD
- protein processing Source: SGD
- proteolysis Source: SGD
- response to pH Source: SGD
- sporulation resulting in formation of a cellular spore Source: SGD
Protein family/group databases
TCDBi | 3.A.31.1.1, the endosomal sorting complexes required for transport iii (escrt-iii) family |
Names & Taxonomyi
Protein namesi | Recommended name: pH-response regulator protein palA/RIM20Alternative name(s): Regulator of IME2 protein 20 |
Gene namesi | Name:RIM20 Ordered Locus Names:YOR275C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005801, RIM20 |
VEuPathDBi | FungiDB:YOR275C |
Subcellular locationi
Nucleus
- nucleus Source: SGD
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 292 – 293 | DN → AA in RIM20-292; prevents cleavage of RIM101. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000218885 | 2 – 661 | pH-response regulator protein palA/RIM20Add BLAST | 660 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | Q12033 |
PaxDbi | Q12033 |
PRIDEi | Q12033 |
PTM databases
iPTMneti | Q12033 |
Interactioni
Subunit structurei
Interacts with SNF7 with its BRO1 domain.
Interacts with RIM101 with its C-terminal domain, probably binding to its two YPX[LI] motifs.
2 PublicationsBinary interactionsi
Q12033
With | #Exp. | IntAct |
---|---|---|
RIM101 [P33400] | 3 | EBI-38947,EBI-14422 |
Protein-protein interaction databases
BioGRIDi | 34664, 516 interactors |
DIPi | DIP-998N |
IntActi | Q12033, 2 interactors |
STRINGi | 4932.YOR275C |
Miscellaneous databases
RNActi | Q12033, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 371 | BRO1PROSITE-ProRule annotationAdd BLAST | 369 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 388 – 422 | Sequence analysisAdd BLAST | 35 |
Sequence similaritiesi
Belongs to the palA/RIM20 family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2220, Eukaryota |
GeneTreei | ENSGT00940000163083 |
HOGENOMi | CLU_007181_3_1_1 |
InParanoidi | Q12033 |
OMAi | AYFKYKE |
Family and domain databases
Gene3Di | 1.25.40.280, 1 hit |
InterProi | View protein in InterPro IPR025304, ALIX_V_dom IPR045251, BRO1-like IPR004328, BRO1_dom IPR038499, BRO1_sf |
PANTHERi | PTHR23030, PTHR23030, 1 hit |
Pfami | View protein in Pfam PF13949, ALIX_LYPXL_bnd, 2 hits PF03097, BRO1, 1 hit |
SMARTi | View protein in SMART SM01041, BRO1, 1 hit |
PROSITEi | View protein in PROSITE PS51180, BRO1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q12033-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSELLAIPLK RTLEVDFATE LSKLIDTTSF QTASFFQSDI LKVVDARNNA
60 70 80 90 100
IAPDISIDGL SALKEYYVIL LQLEKKFPNN QIEFTWFQTL SQKSRGTSQY
110 120 130 140 150
SLQWEKLTII YNIGCMYSLL ALNSNNDAAE SLKTSCLYFQ NAAGCFKHVL
160 170 180 190 200
DHQKNLETIP VVDDATLNAL TSLMLAQAQE CFWFKAVQDK HKDSLIAKLS
210 220 230 240 250
QQIVDFYCEA INDAQRGKLI RSDWINHLKA KKAYFSAVTY YRIALSFNEK
260 270 280 290 300
KQFGNVVKAL QMGLQFINES TLSSQAKFKT VVESSLKEAQ RDNEFIYLQE
310 320 330 340 350
VPSELPSIKP ALMVKPSSSA TLLPSIKKDE TLFKDLIPIE VMEYCTAYNE
360 370 380 390 400
RQDEYVEQRV TNPLASLNKL LKESLTTFQI PQGLTKVSEA ELSHYQASLN
410 420 430 440 450
NLLINNKNVQ VQLDNIEQIL NEEAFTDNQL RLKHGTLNWT LPESSTTNTA
460 470 480 490 500
YYEKLKKLRG YLDEGSAIDK QTNELFQSID KNLIGSEIRL PESNDPLTNK
510 520 530 540 550
IKMIIQERND YIDRTRRKSS EYRILPKIIT SYKKNGTVDF EPIFIGHLKY
560 570 580 590 600
FDEDLRYVNS TKEENIKLIE EVNLSKKNNP GRSGIEPKKM VRIDPRELYI
610 620 630 640 650
EDLRYSFKLL DEVKENLSAG TAFYENLITS TSNLYNEVQE YDTARRAEKA
660
RLDKSLTFED Q
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89633 Genomic DNA Translation: CAA61781.1 Z75183 Genomic DNA Translation: CAA99500.1 BK006948 Genomic DNA Translation: DAA11041.1 |
PIRi | S67177 |
RefSeqi | NP_014918.1, NM_001183694.1 |
Genome annotation databases
EnsemblFungii | YOR275C_mRNA; YOR275C; YOR275C |
GeneIDi | 854449 |
KEGGi | sce:YOR275C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89633 Genomic DNA Translation: CAA61781.1 Z75183 Genomic DNA Translation: CAA99500.1 BK006948 Genomic DNA Translation: DAA11041.1 |
PIRi | S67177 |
RefSeqi | NP_014918.1, NM_001183694.1 |
3D structure databases
SMRi | Q12033 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34664, 516 interactors |
DIPi | DIP-998N |
IntActi | Q12033, 2 interactors |
STRINGi | 4932.YOR275C |
Protein family/group databases
TCDBi | 3.A.31.1.1, the endosomal sorting complexes required for transport iii (escrt-iii) family |
PTM databases
iPTMneti | Q12033 |
Proteomic databases
MaxQBi | Q12033 |
PaxDbi | Q12033 |
PRIDEi | Q12033 |
Genome annotation databases
EnsemblFungii | YOR275C_mRNA; YOR275C; YOR275C |
GeneIDi | 854449 |
KEGGi | sce:YOR275C |
Organism-specific databases
SGDi | S000005801, RIM20 |
VEuPathDBi | FungiDB:YOR275C |
Phylogenomic databases
eggNOGi | KOG2220, Eukaryota |
GeneTreei | ENSGT00940000163083 |
HOGENOMi | CLU_007181_3_1_1 |
InParanoidi | Q12033 |
OMAi | AYFKYKE |
Miscellaneous databases
PROi | PR:Q12033 |
RNActi | Q12033, protein |
Family and domain databases
Gene3Di | 1.25.40.280, 1 hit |
InterProi | View protein in InterPro IPR025304, ALIX_V_dom IPR045251, BRO1-like IPR004328, BRO1_dom IPR038499, BRO1_sf |
PANTHERi | PTHR23030, PTHR23030, 1 hit |
Pfami | View protein in Pfam PF13949, ALIX_LYPXL_bnd, 2 hits PF03097, BRO1, 1 hit |
SMARTi | View protein in SMART SM01041, BRO1, 1 hit |
PROSITEi | View protein in PROSITE PS51180, BRO1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PALA_YEAST | |
Accessioni | Q12033Primary (citable) accession number: Q12033 Secondary accession number(s): D6W2X5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 21, 2005 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - SIMILARITY comments
Index of protein domains and families