UniProtKB - Q12006 (PFA4_YEAST)
Protein
Palmitoyltransferase PFA4
Gene
PFA4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Mediates the reversible addition of palmitate to target proteins, thereby regulating their membrane association and biological function. Palmitoylates several amino acid permeases (PubMed:16751107). Palmitoylates chitin synthase CHS3, which is required for its proper export from the ER (PubMed:16818716, PubMed:28346351). Can palmitoylate RAS2 in vitro (PubMed:12379641).UniRule annotation4 Publications
Catalytic activityi
- hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-hexadecanoyl-L-cysteinyl-[protein]UniRule annotationEC:2.3.1.225UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 108 | S-palmitoyl cysteine intermediateUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- palmitoyltransferase activity Source: UniProtKB
- protein-cysteine S-palmitoyltransferase activity Source: GO_Central
GO - Biological processi
- peptidyl-L-cysteine S-palmitoylation Source: GO_Central
- protein palmitoylation Source: UniProtKB
- protein targeting to membrane Source: GO_Central
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-SCE-203615, eNOS activation |
Names & Taxonomyi
Protein namesi | Recommended name: Palmitoyltransferase PFA41 PublicationUniRule annotation (EC:2.3.1.225UniRule annotation1 Publication)Alternative name(s): Protein S-acyltransferase1 PublicationUniRule annotation Short name: PATUniRule annotation Protein fatty acyltransferase 4UniRule annotation |
Gene namesi | Ordered Locus Names:YOL003CImported ORF Names:UNE378 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005363, PFA4 |
VEuPathDBi | FungiDB:YOL003C |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | CytoplasmicUniRule annotation1 Publication | 9 | |
Transmembranei | 10 – 30 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 31 – 40 | LumenalUniRule annotation1 Publication | 10 | |
Transmembranei | 41 – 61 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 62 – 119 | CytoplasmicUniRule annotation1 PublicationAdd BLAST | 58 | |
Transmembranei | 120 – 140 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 141 – 164 | LumenalUniRule annotation1 PublicationAdd BLAST | 24 | |
Transmembranei | 165 – 185 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 186 – 378 | CytoplasmicUniRule annotation1 PublicationAdd BLAST | 193 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
Leads to the retention of non-palmitoylated CHS3 in the endoplasmic reticulum and reduced levels of chitin on the cell wall.2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 108 | C → A: Causes mislocalization of chitin synthase CHS3 (PubMed:16818716). Reduces, but does not abolish catalytic activity. Lacks autopalmitoylation (PubMed:26224664). 2 Publications | 1 | |
Mutagenesisi | 108 | C → R: Reduces, but does not abolish catalytic activity. Lacks autopalmitoylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212972 | 1 – 378 | Palmitoyltransferase PFA4Add BLAST | 378 |
Post-translational modificationi
Autopalmitoylated.2 Publications
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
MaxQBi | Q12006 |
PaxDbi | Q12006 |
PRIDEi | Q12006 |
PTM databases
iPTMneti | Q12006 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 34401, 137 interactors |
DIPi | DIP-4248N |
IntActi | Q12006, 3 interactors |
STRINGi | 4932.YOL003C |
Miscellaneous databases
RNActi | Q12006, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 78 – 128 | DHHCPROSITE-ProRule annotationAdd BLAST | 51 |
Domaini
The DHHC domain is required for palmitoyltransferase activity.UniRule annotation
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1314, Eukaryota |
HOGENOMi | CLU_027721_8_0_1 |
InParanoidi | Q12006 |
OMAi | AIYTNPG |
Family and domain databases
HAMAPi | MF_03199, DHHC_PAT_PFA4, 1 hit |
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC IPR033682, PFA4 |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12006-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPVKLRWPWL GIAIPTFLIS FIGYGAHYFI LSNFLSVPKQ ITFEFCLSMI
60 70 80 90 100
WLSYYLAICT NPGRPLPNYK PPPDIWRNFC KKCQSYKPER SHHCKTCNQC
110 120 130 140 150
VLMMDHHCPW TMNCVGFANY PHFLRFLFWI IVTTSVLFCI QAKRIYFIWQ
160 170 180 190 200
QRHLPGYFFK KSELIFLTIS SPLNSFVLLT ITILFLRCLF NQILNGRSQI
210 220 230 240 250
ESWDMDRLES LFNSGRLTQK LIDNTWRIYP ESRSFQNKKD AEEHLTKKRP
260 270 280 290 300
RFDELVNFPY DFDLYTNALL YLGPIHLWLW PYGVPTGDGN NFPKNGISKY
310 320 330 340 350
EANSSLEDHI LSLPWPPDGG KTNTVFNHGS STIEMRNESG EQLIRTRLPQ
360 370
NGRHASREKW YNDWGESLDD FGVDVDME
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43491 Genomic DNA Translation: AAC49477.1 Z74745 Genomic DNA Translation: CAA99002.1 AY558023 Genomic DNA Translation: AAS56349.1 BK006948 Genomic DNA Translation: DAA10780.1 |
PIRi | S61981 |
RefSeqi | NP_014640.1, NM_001183257.1 |
Genome annotation databases
EnsemblFungii | YOL003C_mRNA; YOL003C; YOL003C |
GeneIDi | 854159 |
KEGGi | sce:YOL003C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43491 Genomic DNA Translation: AAC49477.1 Z74745 Genomic DNA Translation: CAA99002.1 AY558023 Genomic DNA Translation: AAS56349.1 BK006948 Genomic DNA Translation: DAA10780.1 |
PIRi | S61981 |
RefSeqi | NP_014640.1, NM_001183257.1 |
3D structure databases
SMRi | Q12006 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34401, 137 interactors |
DIPi | DIP-4248N |
IntActi | Q12006, 3 interactors |
STRINGi | 4932.YOL003C |
PTM databases
iPTMneti | Q12006 |
Proteomic databases
MaxQBi | Q12006 |
PaxDbi | Q12006 |
PRIDEi | Q12006 |
Genome annotation databases
EnsemblFungii | YOL003C_mRNA; YOL003C; YOL003C |
GeneIDi | 854159 |
KEGGi | sce:YOL003C |
Organism-specific databases
SGDi | S000005363, PFA4 |
VEuPathDBi | FungiDB:YOL003C |
Phylogenomic databases
eggNOGi | KOG1314, Eukaryota |
HOGENOMi | CLU_027721_8_0_1 |
InParanoidi | Q12006 |
OMAi | AIYTNPG |
Enzyme and pathway databases
Reactomei | R-SCE-203615, eNOS activation |
Miscellaneous databases
PROi | PR:Q12006 |
RNActi | Q12006, protein |
Family and domain databases
HAMAPi | MF_03199, DHHC_PAT_PFA4, 1 hit |
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC IPR033682, PFA4 |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PFA4_YEAST | |
Accessioni | Q12006Primary (citable) accession number: Q12006 Secondary accession number(s): D6W264 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - SIMILARITY comments
Index of protein domains and families