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UniProtKB - Q12001 (ALG6_YEAST)
Protein
Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
Gene
ALG6
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc2-PP-Dol.
1 PublicationCatalytic activityi
- α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl β-D-glucosyl phosphate = α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl phosphate + H+1 PublicationEC:2.4.1.2671 Publication
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity Source: SGD
- hexosyltransferase activity Source: SGD
GO - Biological processi
- aerobic respiration Source: SGD
- dolichol-linked oligosaccharide biosynthetic process Source: InterPro
- oligosaccharide-lipid intermediate biosynthetic process Source: SGD
- protein glycosylation Source: SGD
- protein N-linked glycosylation Source: GO_Central
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.4.1.267, 984 |
Reactomei | R-SCE-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT57, Glycosyltransferase Family 57 |
Names & Taxonomyi
Protein namesi | Recommended name: Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.2671 Publication)Alternative name(s): Asparagine-linked glycosylation protein 6 Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase |
Gene namesi | Name:ALG6 Ordered Locus Names:YOR002W ORF Names:UNA544 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005528, ALG6 |
VEuPathDBi | FungiDB:YOR002W |
Subcellular locationi
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 35 | CytoplasmicSequence analysisAdd BLAST | 35 | |
Transmembranei | 36 – 56 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 57 – 104 | LumenalSequence analysisAdd BLAST | 48 | |
Transmembranei | 105 – 125 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 126 – 145 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 146 – 166 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 167 – 171 | LumenalSequence analysis | 5 | |
Transmembranei | 172 – 192 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 193 – 223 | CytoplasmicSequence analysisAdd BLAST | 31 | |
Transmembranei | 224 – 244 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 245 – 263 | LumenalSequence analysisAdd BLAST | 19 | |
Transmembranei | 264 – 284 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 285 – 332 | CytoplasmicSequence analysisAdd BLAST | 48 | |
Transmembranei | 333 – 353 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 354 – 356 | LumenalSequence analysis | 3 | |
Transmembranei | 357 – 377 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 378 – 398 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 399 – 419 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 420 – 425 | LumenalSequence analysis | 6 | |
Transmembranei | 426 – 446 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 447 – 481 | CytoplasmicSequence analysisAdd BLAST | 35 | |
Transmembranei | 482 – 502 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 503 – 508 | LumenalSequence analysis | 6 | |
Transmembranei | 509 – 529 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 530 – 544 | CytoplasmicSequence analysisAdd BLAST | 15 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000174161 | 1 – 544 | Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferaseAdd BLAST | 544 |
Proteomic databases
PaxDbi | Q12001 |
PRIDEi | Q12001 |
PTM databases
iPTMneti | Q12001 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 34405, 342 interactors |
STRINGi | 4932.YOR002W |
Miscellaneous databases
RNActi | Q12001, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q12001 |
SMRi | Q12001 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the ALG6/ALG8 glucosyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2575, Eukaryota |
GeneTreei | ENSGT00940000153733 |
HOGENOMi | CLU_008110_2_1_1 |
InParanoidi | Q12001 |
OMAi | RQWYFNT |
Family and domain databases
InterProi | View protein in InterPro IPR039488, ALG6 IPR004856, Glyco_trans_ALG6/ALG8 |
PANTHERi | PTHR12413, PTHR12413, 1 hit PTHR12413:SF1, PTHR12413:SF1, 1 hit |
Pfami | View protein in Pfam PF03155, Alg6_Alg8, 1 hit |
i Sequence
Sequence statusi: Complete.
Q12001-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAIGKRLLVN KPAEESFYAS PMYDFLYPFR PVGNQWLPEY IIFVCAVILR
60 70 80 90 100
CTIGLGPYSG KGSPPLYGDF EAQRHWMEIT QHLPLSKWYW YDLQYWGLDY
110 120 130 140 150
PPLTAFHSYL LGLIGSFFNP SWFALEKSRG FESPDNGLKT YMRSTVIISD
160 170 180 190 200
ILFYFPAVIY FTKWLGRYRN QSPIGQSIAA SAILFQPSLM LIDHGHFQYN
210 220 230 240 250
SVMLGLTAYA INNLLDEYYA MAAVCFVLSI CFKQMALYYA PIFFAYLLSR
260 270 280 290 300
SLLFPKFNIA RLTVIAFATL ATFAIIFAPL YFLGGGLKNI HQCIHRIFPF
310 320 330 340 350
ARGIFEDKVA NFWCVTNVFV KYKERFTIQQ LQLYSLIATV IGFLPAMIMT
360 370 380 390 400
LLHPKKHLLP YVLIACSMSF FLFSFQVHEK TILIPLLPIT LLYSSTDWNV
410 420 430 440 450
LSLVSWINNV ALFTLWPLLK KDGLHLQYAV SFLLSNWLIG NFSFITPRFL
460 470 480 490 500
PKSLTPGPSI SSINSDYRRR SLLPYNVVWK SFIIGTYIAM GFYHFLDQFV
510 520 530 540
APPSKYPDLW VLLNCAVGFI CFSIFWLWSY YKIFTSGSKS MKDL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 203 | M → V in AAT92914 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43491 Genomic DNA Translation: AAC49481.1 Z74910 Genomic DNA Translation: CAA99190.1 AY692895 Genomic DNA Translation: AAT92914.1 BK006948 Genomic DNA Translation: DAA10784.1 |
PIRi | S61985 |
RefSeqi | NP_014644.1, NM_001183421.1 |
Genome annotation databases
EnsemblFungii | YOR002W_mRNA; YOR002W; YOR002W |
GeneIDi | 854163 |
KEGGi | sce:YOR002W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43491 Genomic DNA Translation: AAC49481.1 Z74910 Genomic DNA Translation: CAA99190.1 AY692895 Genomic DNA Translation: AAT92914.1 BK006948 Genomic DNA Translation: DAA10784.1 |
PIRi | S61985 |
RefSeqi | NP_014644.1, NM_001183421.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6SNH | electron microscopy | 3.90 | X | 1-544 | [»] | |
6SNI | electron microscopy | 3.00 | X | 1-544 | [»] | |
AlphaFoldDBi | Q12001 | |||||
SMRi | Q12001 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34405, 342 interactors |
STRINGi | 4932.YOR002W |
Protein family/group databases
CAZyi | GT57, Glycosyltransferase Family 57 |
PTM databases
iPTMneti | Q12001 |
Proteomic databases
PaxDbi | Q12001 |
PRIDEi | Q12001 |
Protocols and materials databases
ABCDi | Q12001, 1 sequenced antibody |
Genome annotation databases
EnsemblFungii | YOR002W_mRNA; YOR002W; YOR002W |
GeneIDi | 854163 |
KEGGi | sce:YOR002W |
Organism-specific databases
SGDi | S000005528, ALG6 |
VEuPathDBi | FungiDB:YOR002W |
Phylogenomic databases
eggNOGi | KOG2575, Eukaryota |
GeneTreei | ENSGT00940000153733 |
HOGENOMi | CLU_008110_2_1_1 |
InParanoidi | Q12001 |
OMAi | RQWYFNT |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.267, 984 |
Reactomei | R-SCE-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
Miscellaneous databases
PROi | PR:Q12001 |
RNActi | Q12001, protein |
Family and domain databases
InterProi | View protein in InterPro IPR039488, ALG6 IPR004856, Glyco_trans_ALG6/ALG8 |
PANTHERi | PTHR12413, PTHR12413, 1 hit PTHR12413:SF1, PTHR12413:SF1, 1 hit |
Pfami | View protein in Pfam PF03155, Alg6_Alg8, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ALG6_YEAST | |
Accessioni | Q12001Primary (citable) accession number: Q12001 Secondary accession number(s): D6W268, E9P8Y7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families