UniProtKB - Q11213 (CAPSD_PAVCB)
Protein
Capsid protein VP1
Gene
N/A
Organism
Canine parvovirus type 2 (isolate Dog/United States/CPV-b/1978) (CPV-2)
Status
Functioni
Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptor TFRC. This attachment induces virion internalization predominantly through clathrin-endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell. Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal probably allows nuclear import of capsids (By similarity).By similarity
Miscellaneous
The capsids of autonomous parvoviruses expose a proportion of VP2 N-terminus and part of that sequence can be cleaved of to form VP3.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 323 | Magnesium 1By similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- structural molecule activity Source: InterPro
GO - Biological processi
- adhesion receptor-mediated virion attachment to host cell Source: UniProtKB-KW
- clathrin-dependent endocytosis of virus by host cell Source: UniProtKB-KW
- entry receptor-mediated virion attachment to host cell Source: UniProtKB-KW
- microtubule-dependent intracellular transport of viral material towards nucleus Source: UniProtKB-KW
- permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
- viral entry via permeabilization of inner membrane Source: UniProtKB-KW
- viral penetration into host nucleus Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Capsid protein VP1Alternative name(s): Coat protein VP1 |
Organismi | Canine parvovirus type 2 (isolate Dog/United States/CPV-b/1978) (CPV-2) |
Taxonomic identifieri | 59284 [NCBI] |
Taxonomic lineagei | Viruses › Monodnaviria › Shotokuvirae › Cossaviricota › Quintoviricetes › Piccovirales › Parvoviridae › Parvovirinae › Protoparvovirus › |
Virus hosti | Canis lupus familiaris (Dog) (Canis familiaris) [TaxID: 9615] Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685] |
Subcellular locationi
- Virion By similarity
- Host nucleus Curated
Keywords - Cellular componenti
Capsid protein, Host nucleus, T=1 icosahedral capsid protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000039427 | 1 – 727 | Capsid protein VP1Add BLAST | 727 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 633 ↔ 637 | By similarity |
Keywords - PTMi
Disulfide bondInteractioni
Subunit structurei
Interacts with host TFRC.
By similarityStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q11213 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q11213 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 19 – 64 | Phospholipase A2-likeBy similarityAdd BLAST | 46 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 4 – 13 | Nuclear localization signalSequence analysis | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 165 – 182 | Gly-richAdd BLAST | 18 |
Domaini
The N-terminus of VP1 is sequestered within the mature capsid. It contains a phospholipase A2-like region and nuclear localization signals that might be exposed by capsid modifications during virus entry (By similarity).By similarity
Sequence similaritiesi
Belongs to the parvoviridae capsid protein family.Curated
Family and domain databases
Gene3Di | 2.170.30.10, 1 hit |
InterProi | View protein in InterPro IPR016184, Capsid/spike_ssDNA_virus IPR001403, Parvovirus_coat IPR013607, Parvovirus_coat_VP1_N IPR036952, VP1/VP2 |
Pfami | View protein in Pfam PF00740, Parvo_coat, 1 hit PF08398, Parvo_coat_N, 1 hit |
SUPFAMi | SSF88645, SSF88645, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform VP1 (identifier: Q11213-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPPAKRARR GLVPPGYKYL GPGNSLDQGE PTNPSDAAAK EHDEAYAAYL
60 70 80 90 100
RSGKNPYLYF SPADQRFIDQ TKDAKDWGGK IGHYFFRAKK AIAPVLTDTP
110 120 130 140 150
DHPSTSRPTK PTKRSKPPPH IFINLAKKKK AGAGQVKRDN LAPMSDGAVQ
160 170 180 190 200
PDGGQPAVRN ERATGSGNGS GGGGGGGSGG VGISTGTFNN QTEFKFLENG
210 220 230 240 250
WVEITANSSR LVHLNMPESE NYRRVVVNNM DKTAVNGNMA LDDIHAQIVT
260 270 280 290 300
PWSLVDANAW GVWFNPGDWQ LIVNTMSELH LVSFEQEIFN VVLKTVSESA
310 320 330 340 350
TQPPTKVYNN DLTASLMVAL DSNNTMPFTP AAMRSETLGF YPWKPTIPTP
360 370 380 390 400
WRYYFQWDRT LIPSHTGTSG TPTNIYHGTD PDDVQFYTIE NSVPVHLLRT
410 420 430 440 450
GDEFATGTFF FDCKPCRLTH TWQTNRALGL PPFLNSLPQS EGATNFGDIG
460 470 480 490 500
VQQDKRRGVT QMGNTNYITE ATIMRPAEVG YSAPYYSFEA STQGPFKTPI
510 520 530 540 550
AAGRGGAQTD ENQAADGNPR YAFGRQHGQK TTTTGETPER FTYIAHQDTG
560 570 580 590 600
RYPEGDWIQN INFNLPVTND NVLLPTDPIG GKTGINYTNI FNTYGPLTAL
610 620 630 640 650
NNVPPVYPNG QIWDKEFDTD LKPRLHVNAP FVCQNNCPGQ LFVKVAPNLT
660 670 680 690 700
NEYDPDASAN MSRIVTYSDF WWKGKLVFKA KLRASHTWNP IQQMSINVDN
710 720
QFNYVPSNIG GMKIVYEKSQ LAPRKLY
Note: Minor splicing isoform.
Isoform VP2 (identifier: Q11213-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-143: Missing.
Note: Major splicing isoform produced by deletion of the initiating AUG for VP1 and downstream translation of VP2.Curated
Show »Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041138 | 1 – 143 | Missing in isoform VP2. CuratedAdd BLAST | 143 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M38245 Genomic DNA Translation: AAB02799.1 M38245 Genomic DNA Translation: AAB02800.1 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M38245 Genomic DNA Translation: AAB02799.1 M38245 Genomic DNA Translation: AAB02800.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CAS | X-ray | 3.00 | A | 180-727 | [»] | |
6OAS | electron microscopy | 3.00 | 1 | 180-727 | [»] | |
SMRi | Q11213 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protocols and materials databases
ABCDi | Q11213, 1 sequenced antibody |
Miscellaneous databases
EvolutionaryTracei | Q11213 |
Family and domain databases
Gene3Di | 2.170.30.10, 1 hit |
InterProi | View protein in InterPro IPR016184, Capsid/spike_ssDNA_virus IPR001403, Parvovirus_coat IPR013607, Parvovirus_coat_VP1_N IPR036952, VP1/VP2 |
Pfami | View protein in Pfam PF00740, Parvo_coat, 1 hit PF08398, Parvo_coat_N, 1 hit |
SUPFAMi | SSF88645, SSF88645, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CAPSD_PAVCB | |
Accessioni | Q11213Primary (citable) accession number: Q11213 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 100 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families