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Protein

Puromycin-sensitive aminopeptidase

Gene

Npepps

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins.1 Publication

Caution

It is uncertain whether Met-1 or Met-46 is the initiator.Curated

Catalytic activityi

Release of an N-terminal amino acid, preferentially alanine, from a wide range of peptides, amides and arylamides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Strongly inhibited by bestatin, leuhistin, actinonin, amastatin, 1,10-phenanthroline, DFP, PCMBS, Zn2+, Cd2+, Co2+, Cu2+, Hg2+, EDTA and puromycin. Not inhibited by PMSF, and only slightly inhibited by leupeptin and aprotinin. Activity is increased by Mg2+ and Ca2+ (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei181SubstrateBy similarity1
Metal bindingi353Zinc; catalyticPROSITE-ProRule annotation1
Active sitei354Proton acceptorPROSITE-ProRule annotation1
Metal bindingi357Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi376Zinc; catalyticPROSITE-ProRule annotation1
Sitei439Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Protein family/group databases

MEROPSiM01.010

Names & Taxonomyi

Protein namesi
Recommended name:
Puromycin-sensitive aminopeptidase (EC:3.4.11.14)
Short name:
PSA
Alternative name(s):
Cytosol alanyl aminopeptidase
Short name:
AAP-S
Gene namesi
Name:Npepps
Synonyms:Psa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1101358 Npepps

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice exhibit dwarfism, increased anxiety, impaired pain responses and do not reproduce as well as wild-type mice. More MHC class I molecules are displayed on dendritic cell surfaces.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000951171 – 920Puromycin-sensitive aminopeptidaseAdd BLAST920

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei465Nitrated tyrosineCombined sources1

Keywords - PTMi

Nitration

Proteomic databases

EPDiQ11011
MaxQBiQ11011
PaxDbiQ11011
PeptideAtlasiQ11011
PRIDEiQ11011

PTM databases

iPTMnetiQ11011
PhosphoSitePlusiQ11011
SwissPalmiQ11011

Expressioni

Tissue specificityi

Widely expressed. Highest expression in brain, particularly the striatum and hippocampus. Expressed in Sertoli cells.3 Publications

Gene expression databases

BgeeiENSMUSG00000001441
CleanExiMM_NPEPPS
ExpressionAtlasiQ11011 baseline and differential
GenevisibleiQ11011 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi202409, 2 interactors
IntActiQ11011, 3 interactors
MINTiQ11011
STRINGi10090.ENSMUSP00000001480

Structurei

3D structure databases

ProteinModelPortaliQ11011
SMRiQ11011
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni317 – 321Substrate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi727 – 731Nuclear localization signalSequence analysis5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiKOG1046 Eukaryota
COG0308 LUCA
GeneTreeiENSGT00760000119082
HOGENOMiHOG000106482
HOVERGENiHBG106325
InParanoidiQ11011
KOiK08776
OMAiMMEYVAI
OrthoDBiEOG091G01GH
PhylomeDBiQ11011
TreeFamiTF300395

Family and domain databases

CDDicd09601 M1_APN_2, 1 hit
InterProiView protein in InterPro
IPR024571 ERAP1-like_C_dom
IPR034016 M1_APN-typ
IPR001930 Peptidase_M1
IPR014782 Peptidase_M1_N
PANTHERiPTHR11533 PTHR11533, 1 hit
PfamiView protein in Pfam
PF11838 ERAP1_C, 1 hit
PF01433 Peptidase_M1, 1 hit
PRINTSiPR00756 ALADIPTASE
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q11011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLAAAVPSL ARRLLLLGPP PPPLLLLLSR SSRRRRRLHS LGLAAMPEKR
60 70 80 90 100
PFERLPAEVS PINYSLCLKP DLLDFTFEGK LEAAAQVRQA TNQIVMNCAD
110 120 130 140 150
IDIITASYAP EGDEEIHATG FNYQNEDEKV TLSFPSTLQT GTGTLKIDFV
160 170 180 190 200
GELNDKMKGF YRSRYTTPAG EVRYAAVTQF EATDARRAFP CWDEPAIKAT
210 220 230 240 250
FDISLVVPKD RVALSNMNVI DRKPYPDDEN LVEVKFARTP VMSTYLVAFV
260 270 280 290 300
VGEYDFVETR SKDGVCVRVY TPVGKAEQGK FALEVAAKTL PFYKDYFNVP
310 320 330 340 350
YPLPKIDLIA IADFAAGAME NWGLVTYRET ALLIDPKNSC SSSRQWVALV
360 370 380 390 400
VGHELAHQWF GNLVTMEWWT HLWLNEGFAS WIEYLCVDHC FPEYDIWTQF
410 420 430 440 450
VSADYTRAQE LDALDNSHPI EVSVGHPSEV DEIFDAISYS KGASVIRMLH
460 470 480 490 500
DYIGDKDFKK GMNMYLTKFQ QKNAATEDLW ESLESASGKP IAAVMNTWTK
510 520 530 540 550
QMGFPLIYVE AEQVEDDRVL KLSQKKFCAS GPYGGEDCPQ WMVPITISTS
560 570 580 590 600
EDPNQAKLKI LMDKPEMSVV LKNVKPDQWV KLNLGTVGFY RTQYSSAMLE
610 620 630 640 650
SLLPGIRDLS LPPVDRLGLQ NDLFSLARAG IISTVEVLKV MEAFVNEPNY
660 670 680 690 700
TVWSDLSCNL GILSTLLSHT DFYEEIQEFV KDVFSPIGER LGWDPKPGEG
710 720 730 740 750
HLDALLRGLV LGKLGKAGHK ATLEEARRRF KEHVEGKQIL SADLRSPVYL
760 770 780 790 800
TVLKHGDGAT LDIMLKLHKQ ADMQEEKNRI ERVLGATLSP ELIQKVLTFA
810 820 830 840 850
LSEEVRPQDT VSVIGGVAGG SKHGRKAAWK FIKDNWEELH NRYQGGFLIS
860 870 880 890 900
RLIKLSVEGF AVDKMAGEVK AFFESHPAPS AERTIQQCCE NILLNAAWLK
910 920
RDADSIHQYL LQRKTSPPSV
Length:920
Mass (Da):103,325
Last modified:July 13, 2010 - v2
Checksum:iBECF4139F074A356
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185A → P in AAC52409 (PubMed:7592939).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35646 mRNA Translation: AAC52409.1
AL627445 Genomic DNA Translation: CAM26998.1
CH466556 Genomic DNA Translation: EDL16075.1
BC009653 mRNA Translation: AAH09653.1
BC086798 mRNA Translation: AAH86798.1
BC098212 mRNA Translation: AAH98212.1
AK133898 mRNA Translation: BAE21917.1
CCDSiCCDS25317.1
PIRiT10052
RefSeqiNP_032968.2, NM_008942.2
UniGeneiMm.29824

Genome annotation databases

EnsembliENSMUST00000001480; ENSMUSP00000001480; ENSMUSG00000001441
GeneIDi19155
KEGGimmu:19155
UCSCiuc007ldv.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPSA_MOUSE
AccessioniPrimary (citable) accession number: Q11011
Secondary accession number(s): Q3UZE0, Q5PR74, Q91VJ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 13, 2010
Last modified: June 20, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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