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Protein

Galactoside 2-alpha-L-fucosyltransferase 2

Gene

Fut2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the transfer of fucose to the terminal galactose on glycan chains of cell surface glycoproteins and glycolipids (PubMed:11341836, PubMed:11179967). The resulting epitope plays a role in cell-cell interaction including host-microbe interaction (By similarity). Mediates interaction with intestinal microbiota influencing its composition (By similarity). Creates a membrane-associated precursor oligosaccharide Fuc-alpha((1,2)Gal-beta-) called the H antigen which is an essential substrate for the final step in the membrane-associated blood group antigen synthesis pathway (PubMed:11179967).By similarity2 Publications

Miscellaneous

In rat, there are three genes (Fut1/Fta, Fut2/Ftb and Ftc) which encode galactoside 2-L-fucosyltransferase. They are expressed in a tissue-specific manner and Ftc may have no enzymatic activity.

Catalytic activityi

GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R.2 Publications
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R.2 Publications

Kineticsi

  1. KM=33 mM for phenyl-beta-galactose1 Publication
  2. KM=20 mM for N-acetyllactosamine1 Publication
  3. KM=8.3 mM for lacto-N-biose1 Publication
  4. KM=3.8 mM for galacto-N-biose1 Publication
  5. KM=24 µM for GDP-fucose1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.2 Publications
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    GO - Molecular functioni

    • galactoside 2-alpha-L-fucosyltransferase activity Source: RGD

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.69 5301
    UniPathwayi
    UPA00378

    Protein family/group databases

    CAZyiGT11 Glycosyltransferase Family 11

    Chemistry databases

    SwissLipidsiSLP:000000796

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Galactoside 2-alpha-L-fucosyltransferase 2 (EC:2.4.1.3442 Publications, EC:2.4.1.692 Publications)
    Alternative name(s):
    Alpha 1,2-fucosyltransferase
    Alpha 1,2-fucosyltransferase B
    Alpha 1-2 fucosyltransferase
    Alpha(1,2)FT 2
    Fucosyltransferase 2
    GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2
    Secretor blood group alpha-2-fucosyltransferase
    Gene namesi
    Name:Fut2
    Synonyms:Ftb, Sec1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Unplaced

    Organism-specific databases

    RGDi2639 Fut2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 5CytoplasmicSequence analysis5
    Transmembranei6 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini27 – 354LumenalSequence analysisAdd BLAST328

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001491141 – 354Galactoside 2-alpha-L-fucosyltransferase 2Add BLAST354

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi199N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ10984
    PRIDEiQ10984

    Expressioni

    Tissue specificityi

    Specifically expressed in gut.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000028519

    Structurei

    3D structure databases

    SMRiQ10984
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 11 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IFKR Eukaryota
    ENOG4111HG3 LUCA
    HOGENOMiHOG000261621
    HOVERGENiHBG004338
    InParanoidiQ10984
    KOiK00718
    PhylomeDBiQ10984
    TreeFamiTF315810

    Family and domain databases

    InterProiView protein in InterPro
    IPR002516 Glyco_trans_11
    PANTHERiPTHR11927 PTHR11927, 1 hit
    PfamiView protein in Pfam
    PF01531 Glyco_transf_11, 1 hit

    Sequences (2+)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

    Isoform 1 (identifier: Q10984-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MASAQVPFSF PLAHFLIFVF VTSTITHLQQ RIVKLQPLSE KELPMTTQMS
    60 70 80 90 100
    SGNTESPEMR RDSEQHGNGE LRGMFTINSI GRLGNQMGEY ATLFALARMN
    110 120 130 140 150
    GRLAFIPASM HNALAPIFRI SLPVLHSDTA KKIPWQNYHL NDWMEERYRH
    160 170 180 190 200
    IPGHFVRFTG YPCSWTFYHH LRPEILKEFT LHDHVREEAQ AFLRGLRVNG
    210 220 230 240 250
    SQPSTFVGVH VRRGDYVHVM PNVWKGVVAD RGYLEKALDM FRARYSSPVF
    260 270 280 290 300
    VVTSNGMAWC RENINASRGD VVFAGNGIEG SPAKDFALLT QCNHTIMTIG
    310 320 330 340 350
    TFGIWAAYLA GGDTIYLANY TLPDSPFLKV FKPEAAFLPE WVGIPADLSP

    LLKH
    Length:354
    Mass (Da):39,983
    Last modified:December 6, 2005 - v2
    Checksum:i123E8C8379E8559E
    GO
    Isoform 2 (identifier: Q10984-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         354-354: H → ALTPACPRSHFHLKAKGVTCYVAGRAF

    Show »
    Length:380
    Mass (Da):42,730
    Checksum:i8183786AE8212EBE
    GO

    Computationally mapped potential isoform sequencesi

    There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    A0A0G2JSS7A0A0G2JSS7_RAT
    L-Fucosyltransferase
    Fut2 rCG_53914
    354Annotation score:

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti26T → I (PubMed:11341836).Curated1
    Sequence conflicti26T → I (Ref. 3) Curated1
    Sequence conflicti271V → G in AAB41515 (PubMed:8010942).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_016526354H → ALTPACPRSHFHLKAKGVTC YVAGRAF in isoform 2. 2 Publications1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF264005 mRNA Translation: AAF72200.1
    AF131238 Genomic DNA Translation: AAD24469.1
    AB006138 mRNA Translation: BAA21742.1
    L26010 mRNA Translation: AAB41515.1
    PIRiS46494
    RefSeqiNP_113823.1, NM_031635.1
    XP_006229197.1, XM_006229135.3
    UniGeneiRn.10678

    Genome annotation databases

    GeneIDi58924
    KEGGirno:58924
    UCSCiRGD:2639 rat [Q10984-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF264005 mRNA Translation: AAF72200.1
    AF131238 Genomic DNA Translation: AAD24469.1
    AB006138 mRNA Translation: BAA21742.1
    L26010 mRNA Translation: AAB41515.1
    PIRiS46494
    RefSeqiNP_113823.1, NM_031635.1
    XP_006229197.1, XM_006229135.3
    UniGeneiRn.10678

    3D structure databases

    SMRiQ10984
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000028519

    Chemistry databases

    SwissLipidsiSLP:000000796

    Protein family/group databases

    CAZyiGT11 Glycosyltransferase Family 11

    Proteomic databases

    PaxDbiQ10984
    PRIDEiQ10984

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi58924
    KEGGirno:58924
    UCSCiRGD:2639 rat [Q10984-1]

    Organism-specific databases

    CTDi2524
    RGDi2639 Fut2

    Phylogenomic databases

    eggNOGiENOG410IFKR Eukaryota
    ENOG4111HG3 LUCA
    HOGENOMiHOG000261621
    HOVERGENiHBG004338
    InParanoidiQ10984
    KOiK00718
    PhylomeDBiQ10984
    TreeFamiTF315810

    Enzyme and pathway databases

    UniPathwayi
    UPA00378

    BRENDAi2.4.1.69 5301

    Miscellaneous databases

    PROiPR:Q10984

    Family and domain databases

    InterProiView protein in InterPro
    IPR002516 Glyco_trans_11
    PANTHERiPTHR11927 PTHR11927, 1 hit
    PfamiView protein in Pfam
    PF01531 Glyco_transf_11, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiFUT2_RAT
    AccessioniPrimary (citable) accession number: Q10984
    Secondary accession number(s): O35087, Q9JK44, Q9R275
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: December 6, 2005
    Last modified: May 23, 2018
    This is version 123 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
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