UniProtKB - Q10741 (ADA10_BOVIN)
Disintegrin and metalloproteinase domain-containing protein 10
ADAM10
Functioni
Catalytic activityi
- Endopeptidase of broad specificity.By similarity EC:3.4.24.81
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 173 | Zinc; in inhibited formBy similarity | 1 | |
Metal bindingi | 383 | Zinc; via tele nitrogen; catalyticBy similarity | 1 | |
Active sitei | 384 | 1 Publication | 1 | |
Metal bindingi | 387 | Zinc; via tele nitrogen; catalyticBy similarity | 1 | |
Metal bindingi | 393 | Zinc; via tele nitrogen; catalyticBy similarity | 1 |
GO - Molecular functioni
- endopeptidase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- metalloendopeptidase activity Source: UniProtKB
- metalloendopeptidase activity involved in amyloid precursor protein catabolic process Source: GO_Central
- metallopeptidase activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- protein kinase binding Source: UniProtKB
- SH3 domain binding Source: UniProtKB-KW
GO - Biological processi
- adherens junction organization Source: Ensembl
- cochlea development Source: Ensembl
- constitutive protein ectodomain proteolysis Source: Ensembl
- in utero embryonic development Source: UniProtKB
- membrane protein ectodomain proteolysis Source: UniProtKB
- monocyte activation Source: Ensembl
- negative regulation of cell adhesion Source: UniProtKB
- negative regulation of gene expression Source: Ensembl
- Notch signaling pathway Source: UniProtKB
- pore complex assembly Source: Ensembl
- positive regulation of cell growth Source: Ensembl
- positive regulation of cell population proliferation Source: Ensembl
- positive regulation of T cell chemotaxis Source: Ensembl
- postsynapse organization Source: Ensembl
- protein phosphorylation Source: UniProtKB
- protein processing Source: Ensembl
- regulation of neurotransmitter receptor localization to postsynaptic specialization membrane Source: Ensembl
- regulation of Notch signaling pathway Source: Ensembl
- regulation of vasculature development Source: Ensembl
- response to tumor necrosis factor Source: Ensembl
- toxin transport Source: Ensembl
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Biological process | Notch signaling pathway |
Ligand | Metal-binding, Zinc |
Protein family/group databases
MEROPSi | M12.210 |
Names & Taxonomyi
Protein namesi | Recommended name: Disintegrin and metalloproteinase domain-containing protein 10 (EC:3.4.24.81By similarity)Short name: ADAM 10 Alternative name(s): Kuzbanian protein homolog Mammalian disintegrin-metalloprotease Myelin-associated metalloproteinase CD_antigen: CD156c |
Gene namesi | Name:ADAM10 Synonyms:MADM |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:25604, ADAM10 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Single-pass type I membrane protein Curated
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Single-pass type I membrane protein Curated
Other locations
- clathrin-coated vesicle By similarity
- axon By similarity
- dendrite By similarity
- adherens junction By similarity
- Cytoplasm By similarity
Note: Is localized in the plasma membrane but is also expressed in the Golgi apparatus and in clathrin-coated vesicles derived likely from the Golgi (By similarity). During long term depression, it is recruited to the cell membrane by DLG1 (By similarity). The immature form is mainly located near cytoplasmic fibrillar structures, while the mature form is predominantly located at zonula adherens and the cell membrane (By similarity). The localization and clustering of mature ADAM10 to zonula adherens is regulated by AFDN, TSPAN33, PLEKHA7 and PDZD11 (By similarity).By similarity
Endoplasmic reticulum
- perinuclear endoplasmic reticulum Source: Ensembl
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: GO_Central
- synaptic membrane Source: GO_Central
- tetraspanin-enriched microdomain Source: Ensembl
Other locations
- adherens junction Source: UniProtKB-SubCell
- axon Source: UniProtKB-SubCell
- cell surface Source: UniProtKB
- clathrin-coated vesicle Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB
- dendrite Source: UniProtKB-SubCell
- glutamatergic synapse Source: Ensembl
- Golgi-associated vesicle Source: UniProtKB
- pore complex Source: Ensembl
- postsynaptic density Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 20 – 672 | ExtracellularSequence analysisAdd BLAST | 653 | |
Transmembranei | 673 – 696 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 697 – 748 | CytoplasmicSequence analysisAdd BLAST | 52 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 210 – 213 | RKKR → NAQA: Abolishes furin cleavage site, leading to defects in pro. 1 Publication | 4 | |
Mutagenesisi | 384 | E → A: Decreased stimulated and constitutive secretion of APP. 1 Publication | 1 | |
Mutagenesisi | 573 | E → A: Abrogates EFNA5 cleavage; when associated with Ala-578 and 579. 1 Publication | 1 | |
Mutagenesisi | 578 | E → A: Abrogates EFNA5 cleavage; when associated with Ala-573 and 579. 1 Publication | 1 | |
Mutagenesisi | 579 | E → A: Abrogates EFNA5 cleavage; when associated with Ala-573 and 578. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
PropeptideiPRO_0000029064 | 20 – 213 | 2 PublicationsAdd BLAST | 194 | |
ChainiPRO_0000029065 | 214 – 748 | Disintegrin and metalloproteinase domain-containing protein 10Add BLAST | 535 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 267 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 278 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 344 ↔ 451 | By similarity | ||
Disulfide bondi | 399 ↔ 435 | By similarity | ||
Glycosylationi | 439 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 460 ↔ 495 | Combined sources1 Publication | ||
Disulfide bondi | 471 ↔ 484 | Combined sources1 Publication | ||
Disulfide bondi | 473 ↔ 479 | Combined sources1 Publication | ||
Disulfide bondi | 483 ↔ 515 | Combined sources1 Publication | ||
Disulfide bondi | 503 ↔ 511 | Combined sources2 Publications | ||
Disulfide bondi | 510 ↔ 536 | Combined sources2 Publications | ||
Disulfide bondi | 524 ↔ 543 | Combined sources2 Publications | ||
Disulfide bondi | 530 ↔ 562 | Combined sources2 Publications | ||
Glycosylationi | 551 | N-linked (GlcNAc...) asparagineCombined sources | 1 | |
Disulfide bondi | 555 ↔ 567 | Combined sources2 Publications | ||
Disulfide bondi | 572 ↔ 598 | Combined sources2 Publications | ||
Disulfide bondi | 580 ↔ 607 | Combined sources2 Publications | ||
Disulfide bondi | 582 ↔ 597 | Combined sources2 Publications | ||
Disulfide bondi | 594 ↔ 639 | Combined sources2 Publications | ||
Disulfide bondi | 632 ↔ 645 | Combined sources2 Publications | ||
Modified residuei | 719 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 213 – 214 | Cleavage; by furin and PCSK71 Publication | 2 |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Phosphoprotein, ZymogenProteomic databases
PaxDbi | Q10741 |
PeptideAtlasi | Q10741 |
PRIDEi | Q10741 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSBTAG00000005481, Expressed in conceptus and 19 other tissues |
ExpressionAtlasi | Q10741, baseline and differential |
Interactioni
Subunit structurei
Forms a ternary EFNA5-EPHA3-ADAM10 complex mediating EFNA5 extracellular domain shedding by ADAM10 which regulates the EFNA5-EPHA3 complex internalization and function (PubMed:16239146).
Interacts with the clathrin adapter AP2 complex subunits AP2A1, AP2A2, AP2B1, and AP2M1; this interaction facilitates ADAM10 endocytosis from the plasma membrane during long-term potentiation in hippocampal neurons (By similarity).
Interacts with EPHA2 (By similarity).
Interacts (via extracellular domain) with TSPAN33 (via extracellular domain) and (via cytoplasmic domain) with AFDN; interaction with TSPAN33 allows the docking of ADAM10 to zonula adherens through a PDZ11-dependent interaction between TSPAN33 and PLEKHA7 while interaction with AFDN locks ADAM10 at zonula adherens (By similarity).
Forms a ternary complex composed of ADAM10, EPHA4 and CADH1; within the complex, ADAM10 cleaves CADH1 which disrupts adherens junctions (By similarity).
Interacts with NGF in a divalent cation-dependent manner (By similarity).
Interacts with TSPAN14; the interaction promotes ADAM10 maturation and cell surface expression (PubMed:23035126).
Interacts with TSPAN5, TSPAN10, TSPAN15, TSPAN17 and TSPAN33; these interactions regulate ADAM10 substrate specificity (PubMed:23035126).
Interacts with DLG1; this interaction recruits ADAM10 to the cell membrane during long-term depression in hippocampal neurons (By similarity).
Interacts (via extracellular domain) with BACE1 (via extracellular domain) (By similarity).
Interacts with FAM171A1 (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
- protein kinase binding Source: UniProtKB
- SH3 domain binding Source: UniProtKB-KW
Protein-protein interaction databases
DIPi | DIP-48422N |
IntActi | Q10741, 5 interactors |
STRINGi | 9913.ENSBTAP00000042110 |
Structurei
Secondary structure
3D structure databases
SMRi | Q10741 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q10741 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 220 – 456 | Peptidase M12BPROSITE-ProRule annotationAdd BLAST | 237 | |
Domaini | 457 – 551 | DisintegrinPROSITE-ProRule annotationAdd BLAST | 95 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 734 – 748 | Interaction with AP2A1, AP2A2 and AP2M1By similarityAdd BLAST | 15 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 171 – 178 | Cysteine switch | 8 | |
Motifi | 708 – 715 | SH3-bindingSequence analysis | 8 | |
Motifi | 722 – 728 | SH3-bindingSequence analysis | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 555 – 595 | Cys-richCuratedAdd BLAST | 41 |
Domaini
Keywords - Domaini
SH3-binding, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3658, Eukaryota |
GeneTreei | ENSGT00940000160579 |
HOGENOMi | CLU_004602_0_0_1 |
InParanoidi | Q10741 |
OMAi | YTGHIYV |
OrthoDBi | 162519at2759 |
TreeFami | TF352021 |
Family and domain databases
CDDi | cd04270, ZnMc_TACE_like, 1 hit |
Gene3Di | 3.40.390.10, 1 hit 4.10.70.10, 1 hit |
InterProi | View protein in InterPro IPR034025, ADAM10_ADAM17 IPR027053, ADAM_10 IPR001762, Disintegrin_dom IPR036436, Disintegrin_dom_sf IPR024079, MetalloPept_cat_dom_sf IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N |
PANTHERi | PTHR45702:SF4, PTHR45702:SF4, 1 hit |
Pfami | View protein in Pfam PF00200, Disintegrin, 1 hit PF01562, Pep_M12B_propep, 1 hit |
SMARTi | View protein in SMART SM00050, DISIN, 1 hit |
SUPFAMi | SSF57552, SSF57552, 1 hit |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50214, DISINTEGRIN_2, 1 hit PS00142, ZINC_PROTEASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MVLLRVLILL LSWVAGLGGQ YGNPLNKYIR HYEGLSYDVD SLHQKHQRAK
60 70 80 90 100
RAVSHEDQFL RLDFHAHGRH FNLRMKRDTS LFSEEFRVET SNAVLDYDTS
110 120 130 140 150
HIYTGHIYGE EGSFSHGSVI DGRFEGFIQT HGGTFYVEPA ERYIKDRTLP
160 170 180 190 200
FHSVIYHEDD IKYPHKYGPQ GGCADHSVFE RMRKYQMTGV EEVTQTPQEK
210 220 230 240 250
HAINGPELLR KKRTTVAEKN TCQLYIQTDH LFFKYYGTRE AVIAQISSHV
260 270 280 290 300
KAIDTIYQTT DFSGIRNISF MVKRIRINTT ADEKDPTNPF RFPNIGVEKF
310 320 330 340 350
LELNSEQNHD DYCLAYVFTD RDFDDGVLGL AWVGAPSGSS GGICEKSKLY
360 370 380 390 400
SDGKKKSLNT GIITVQNYGS HVPPKVSHIT FAHEVGHNFG SPHDSGTECT
410 420 430 440 450
PGESKNLGQK ENGNYIMYAR ATSGDKLNNN KFSLCSIRNI SQVLEKKRNN
460 470 480 490 500
CFVESGQPIC GNGMVEQGEE CDCGYSDQCK DECCYDANQP EGKKCKLKPG
510 520 530 540 550
KQCSPSQGPC CTAHCAFKSK TEKCRDDSDC AKEGICNGIT ALCPASDPKP
560 570 580 590 600
NFTDCNRHTQ VCINGQCAGS ICEKHGLEEC TCASSDGKDD KELCHVCCMK
610 620 630 640 650
KMEPSTCAST GSVQWNKYFL GRTITLQPGS PCNDFRGYCD VFMRCRLVDA
660 670 680 690 700
DGPLARLKKA IFSPELYENI AEWIVAYWWA VLLMGIALIM LMAGFIKICS
710 720 730 740
VHTPSSNPKL PPPKPLPGTL KRRRPPQPIQ QPQRQRPRES YQMGHMRR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A452DJA5 | A0A452DJA5_BOVIN | Disintegrin and metalloproteinase d... | ADAM10 | 680 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z21961 mRNA Translation: CAA79973.1 BC153863 mRNA Translation: AAI53864.1 |
PIRi | S66129 |
RefSeqi | NP_776921.1, NM_174496.2 |
Genome annotation databases
Ensembli | ENSBTAT00000075105; ENSBTAP00000056919; ENSBTAG00000005481 |
GeneIDi | 282132 |
KEGGi | bta:282132 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z21961 mRNA Translation: CAA79973.1 BC153863 mRNA Translation: AAI53864.1 |
PIRi | S66129 |
RefSeqi | NP_776921.1, NM_174496.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2AO7 | X-ray | 2.90 | A | 455-646 | [»] | |
5L0Q | X-ray | 2.76 | A/D | 455-646 | [»] | |
SMRi | Q10741 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-48422N |
IntActi | Q10741, 5 interactors |
STRINGi | 9913.ENSBTAP00000042110 |
Protein family/group databases
MEROPSi | M12.210 |
Proteomic databases
PaxDbi | Q10741 |
PeptideAtlasi | Q10741 |
PRIDEi | Q10741 |
Protocols and materials databases
ABCDi | Q10741, 1 sequenced antibody |
Genome annotation databases
Ensembli | ENSBTAT00000075105; ENSBTAP00000056919; ENSBTAG00000005481 |
GeneIDi | 282132 |
KEGGi | bta:282132 |
Organism-specific databases
CTDi | 102 |
VGNCi | VGNC:25604, ADAM10 |
Phylogenomic databases
eggNOGi | KOG3658, Eukaryota |
GeneTreei | ENSGT00940000160579 |
HOGENOMi | CLU_004602_0_0_1 |
InParanoidi | Q10741 |
OMAi | YTGHIYV |
OrthoDBi | 162519at2759 |
TreeFami | TF352021 |
Miscellaneous databases
EvolutionaryTracei | Q10741 |
Gene expression databases
Bgeei | ENSBTAG00000005481, Expressed in conceptus and 19 other tissues |
ExpressionAtlasi | Q10741, baseline and differential |
Family and domain databases
CDDi | cd04270, ZnMc_TACE_like, 1 hit |
Gene3Di | 3.40.390.10, 1 hit 4.10.70.10, 1 hit |
InterProi | View protein in InterPro IPR034025, ADAM10_ADAM17 IPR027053, ADAM_10 IPR001762, Disintegrin_dom IPR036436, Disintegrin_dom_sf IPR024079, MetalloPept_cat_dom_sf IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N |
PANTHERi | PTHR45702:SF4, PTHR45702:SF4, 1 hit |
Pfami | View protein in Pfam PF00200, Disintegrin, 1 hit PF01562, Pep_M12B_propep, 1 hit |
SMARTi | View protein in SMART SM00050, DISIN, 1 hit |
SUPFAMi | SSF57552, SSF57552, 1 hit |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50214, DISINTEGRIN_2, 1 hit PS00142, ZINC_PROTEASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADA10_BOVIN | |
Accessioni | Q10741Primary (citable) accession number: Q10741 Secondary accession number(s): A8E663 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 28, 2003 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references