UniProtKB - Q10572 (FOX1_CAEEL)
Sex determination protein fox-1
fox-1
Functioni
RNA-binding protein that regulates tissue-specific alternative splicing events by binding to 5'-UGCAUG-3' and 5'-GCACG-3' elements (PubMed:17060915, PubMed:17923701, PubMed:23468662, PubMed:33372658).
Also binds to poly(A), poly(G), poly(C), or poly(U) stretches of RNA (PubMed:9927456).
Plays a role in the sex determination pathway and X chromosome dosage compensation, and together with sex-1 is involved in making the distinction between one and two X-chromosomes (PubMed:7821230, PubMed:21471153, PubMed:23666922, PubMed:9217163, PubMed:9927456, PubMed:16139225, PubMed:17720939, PubMed:33372658).
Binds to 5'-GCAUG-3' and 5'-GCACG-3' elements in intron 6 of the pre-mRNA of the sex-determining factor xol-1 to promote its alternative splicing and together with sex-1 negatively regulates the expression of xol-1 to promote hermaphrodite development (PubMed:9217163, PubMed:33372658).
Negatively regulates the expression of the active isoform of xol-1 (isoform b) by promoting intron 6 retention and the deletion of exon 7 coding sequences in hermaphrodite embryos (PubMed:33372658).
Furthermore, binding to the pre-mRNA of xol-1 can also direct the use of an alternative 3' splice site enabling the xol-1 transcript to be trans-spliced to unrelated genes on chromosome 2, which also leads to xol-1 exon 7 deletion (PubMed:33372658).
Does not seem to regulate the retention of introns 1 to 5 of xol-1 pre-mRNA (PubMed:33372658).
Plays a role in the association of the dosage compensation complex proteins dpy-27 and sdc-3 with the hermaphrodite X chromosomes (PubMed:16139225, PubMed:17720939).
Binds to 5'-UGCAUG-3' elements in intron 7 of the pre-mRNA of unc-32 to promote its alternative splicing in neuronal tissues (PubMed:23468662).
Binds to 5'-UGCAUG-3' elements in intron 4 of the pre-mRNA of egl-15 to promote its alternative splicing in body wall muscle tissues (PubMed:17060915).
Promotes binding of RNA-binding protein sup-12 to target RNA (PubMed:17923701).
Plays a role in male mating behavior (PubMed:9927456).
11 PublicationsGO - Molecular functioni
- mRNA binding Source: GO_Central
- poly(A) binding Source: WormBase
- poly(G) binding Source: WormBase
- poly(U) RNA binding Source: WormBase
- pre-mRNA intronic binding Source: UniProtKB
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- dosage compensation by hypoactivation of X chromosome Source: UniProtKB
- intergenic mRNA trans splicing Source: UniProtKB
- mating behavior Source: WormBase
- mRNA metabolic process Source: WormBase
- negative regulation of gene expression Source: UniProtKB
- nervous system development Source: GO_Central
- primary sex determination Source: UniProtKB
- regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
- regulation of gene expression Source: UniProtKB
- sex differentiation Source: UniProtKB-KW
Keywordsi
Molecular function | RNA-binding |
Biological process | Differentiation, Sexual differentiation, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Sex determination protein fox-1Alternative name(s): Feminizing locus on X protein 1 |
Gene namesi | Name:fox-1Imported ORF Names:T07D1.4Imported |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | T07D1.4a ; CE51693 ; WBGene00001484 ; fox-1 T07D1.4b ; CE51620 ; WBGene00001484 ; fox-1 T07D1.4d ; CE51563 ; WBGene00001484 ; fox-1 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication2 Publications
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 423 – 429 | Missing : Disrupts nuclear localization. 1 Publication | 7 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081589 | 1 – 429 | Sex determination protein fox-1Add BLAST | 429 |
Proteomic databases
EPDi | Q10572 |
PaxDbi | Q10572 |
PeptideAtlasi | Q10572 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | WBGene00001484, Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues |
ExpressionAtlasi | Q10572, baseline and differential |
Interactioni
Subunit structurei
Interacts with sup-12.
1 PublicationProtein-protein interaction databases
BioGRIDi | 45493, 3 interactors |
IntActi | Q10572, 2 interactors |
STRINGi | 6239.T07D1.4 |
Structurei
3D structure databases
AlphaFoldDBi | Q10572 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 183 – 259 | RRMPROSITE-ProRule annotationAdd BLAST | 77 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 156 – 188 | DisorderedSequence analysisAdd BLAST | 33 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 156 – 177 | Polar residuesSequence analysisAdd BLAST | 22 |
Phylogenomic databases
eggNOGi | KOG0125, Eukaryota |
GeneTreei | ENSGT00940000168999 |
HOGENOMi | CLU_662647_0_0_1 |
InParanoidi | Q10572 |
OrthoDBi | 871288at2759 |
Family and domain databases
CDDi | cd12407, RRM_FOX1_like, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR034237, FOX1_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAELIEEVST ATHASLNACD PIVVNSEALP MQALYQLSAT GAQQQNQQIP
60 70 80 90 100
IGLSNSLLYQ QLAAHQQIAA QQHQQQLAVS AAHQTQNNIM LATSAPSLIN
110 120 130 140 150
HMENSTDGKV KDDPNSDYDL QLSIQQQLAA AAQAAQMGQT QIGPQIVGQQ
160 170 180 190 200
GQPVVATTAG STNGSAAVTQ PDPSTSSGPD GPKRLHVSNI PFRFRDPDLK
210 220 230 240 250
TMFEKFGVVS DVEIIFNERG SKGFGFVTME RPQDAERARQ ELHGSMIEGR
260 270 280 290 300
KIEVNCATAR VHSKKVKPTG GILDQMNPLM AQSALAAQAQ MNRALLLRSP
310 320 330 340 350
LVAQSLLGRG AALIPGMQQP AFQLQAALAG NPLAQLQGQP LLFNAAALQT
360 370 380 390 400
NALQQSAFGM DPAAVQAALL ANEQARFQLA AAAAQGRIPS SGNASAFGEQ
410 420
YLSNALATAS LPSYQMNPAL RTLNRFTPY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 127 | Q → R in AAA67723 (PubMed:7821230).Curated | 1 | |
Sequence conflicti | 311 | A → P in AAA67723 (PubMed:7821230).Curated | 1 | |
Sequence conflicti | 366 | Q → L in AAA67723 (PubMed:7821230).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060629 | 1 – 89 | Missing in isoform b. CuratedAdd BLAST | 89 | |
Alternative sequenceiVSP_060630 | 1 – 30 | Missing in isoform a. CuratedAdd BLAST | 30 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14946 mRNA Translation: AAA67723.1 BX284606 Genomic DNA Translation: CCD67194.1 BX284606 Genomic DNA Translation: SAP35625.1 BX284606 Genomic DNA Translation: SAP35629.1 |
PIRi | T16825 |
RefSeqi | NP_001317738.1, NM_001330895.1 [Q10572-2] NP_001317857.1, NM_001330894.1 [Q10572-1] NP_001317861.1, NM_001330896.1 [Q10572-3] |
Genome annotation databases
EnsemblMetazoai | T07D1.4a.1; T07D1.4a.1; WBGene00001484 [Q10572-2] T07D1.4b.1; T07D1.4b.1; WBGene00001484 [Q10572-3] T07D1.4d.1; T07D1.4d.1; WBGene00001484 [Q10572-1] |
GeneIDi | 180549 |
KEGGi | cel:CELE_T07D1.4 |
UCSCi | T07D1.4.2, c. elegans [Q10572-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14946 mRNA Translation: AAA67723.1 BX284606 Genomic DNA Translation: CCD67194.1 BX284606 Genomic DNA Translation: SAP35625.1 BX284606 Genomic DNA Translation: SAP35629.1 |
PIRi | T16825 |
RefSeqi | NP_001317738.1, NM_001330895.1 [Q10572-2] NP_001317857.1, NM_001330894.1 [Q10572-1] NP_001317861.1, NM_001330896.1 [Q10572-3] |
3D structure databases
AlphaFoldDBi | Q10572 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 45493, 3 interactors |
IntActi | Q10572, 2 interactors |
STRINGi | 6239.T07D1.4 |
Proteomic databases
EPDi | Q10572 |
PaxDbi | Q10572 |
PeptideAtlasi | Q10572 |
Genome annotation databases
EnsemblMetazoai | T07D1.4a.1; T07D1.4a.1; WBGene00001484 [Q10572-2] T07D1.4b.1; T07D1.4b.1; WBGene00001484 [Q10572-3] T07D1.4d.1; T07D1.4d.1; WBGene00001484 [Q10572-1] |
GeneIDi | 180549 |
KEGGi | cel:CELE_T07D1.4 |
UCSCi | T07D1.4.2, c. elegans [Q10572-1] |
Organism-specific databases
CTDi | 180549 |
WormBasei | T07D1.4a ; CE51693 ; WBGene00001484 ; fox-1 T07D1.4b ; CE51620 ; WBGene00001484 ; fox-1 T07D1.4d ; CE51563 ; WBGene00001484 ; fox-1 |
Phylogenomic databases
eggNOGi | KOG0125, Eukaryota |
GeneTreei | ENSGT00940000168999 |
HOGENOMi | CLU_662647_0_0_1 |
InParanoidi | Q10572 |
OrthoDBi | 871288at2759 |
Miscellaneous databases
PROi | PR:Q10572 |
Gene expression databases
Bgeei | WBGene00001484, Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues |
ExpressionAtlasi | Q10572, baseline and differential |
Family and domain databases
CDDi | cd12407, RRM_FOX1_like, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR034237, FOX1_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FOX1_CAEEL | |
Accessioni | Q10572Primary (citable) accession number: Q10572 Secondary accession number(s): A0A168HBS9, A0A168HBX9, Q22304 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2002 |
Last sequence update: | August 12, 2020 | |
Last modified: | May 25, 2022 | |
This is version 137 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase